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ID   PMBL604    preliminary; circular DNA; SYN; 4372 BP.
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AC   L08934; VB0126;
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DT   04-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pMBL604 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-4372
RC   pMBL604
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [2]
RC   pMBL604
RA   Enger-Valk B.E.;
RT   ;
RL   Submitted (00-XXX-1991) on floppy disc by:
RL   Enger-Valk B.E., .
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Revised according to sequence of pBR322 by F. Pfeiffer
CC   This vector contains a fused Tet-trp promoter (p3016) with a
CC   synthetic oligonucleotide containing a ribosome binding site and an
CC   EcoRI site.
CC   The oligonucleotide sequence is ATAGGAATTC.
CC   NM (pMBL604)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(EcoTrp)(EcoGalK)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMBL1 remove, 5208bp-
FT                   2. oligo RBS-EcoRI 10bp ataggaattc, after trp prom
FT                   -> pMBL604 4372bp"
FT   misc_feature    12..43
FT                   /note="from pBR322"
FT   CDS             65..219
FT                   /note="GEN E. coli galactokinase gene (galK)"
FT   misc_feature    231..1438
FT                   /note="from EcoTrp"
FT   misc_feature    1439..4372
FT                   /note="from pBR322"
FT   CDS             complement(3307..4095)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    218..227
FT                   /note="synthetic oligonucleotide ATAGGAATTC"
FT   RBS             220..223
FT                   /note="RBS synthetic ribosome binding site"
XX
SQ   Sequence 4372 BP; 1089 A; 1136 C; 1057 G; 1090 T; 0 other;
     ttatggtacc atctcatgtt tgacagctta tcatcgataa gctaaccttt ccggggcaat
     aagggctgca cgcgcacttt tatccgcctc tgctgcgctc cgccaccgta cgtaaattta
     tggttggtta tgaaatgctg gcagagaccc agcgagacct gaccgcagaa caggcagcag
     agcgtttgcg cgcagtcagc gatatccatt ttcgcgaata ggaattccta aacctttccg
     gtcgcggcga taaagacatc ttcaccgttc acgatatttt gaaagcacga ggggaaatct
     gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag gcgcattcgt
     tcctttcgtc acgctcggtg atccgggcat tgagcagtca ttgaaaatta tcgatacgct
     aattgaagcc ggtgctgacg cgctggagtt aggtatcccc ttctccgacc cactggcgga
     tggcccgacg attcaaaacg ccactctgcg cgcctttgcg gcaggtgtga ctccggcaca
     atgttttgaa atgctggcac tgattcgcca gaaacacccg accattccca ttggcctgtt
     gatgtatgcc aatctggtgt ttaacaaagg cattgatgag ttttatgccc agtgcgaaaa
     agtcggcgtc gattcggtgc tggttgccga tgtgccagtt gaagagtccg cgcccttccg
     ccaggccgcg ttgcgtcaca acgtcgcacc tatcttcatc tgcccgccaa atgccgatga
     cgacctgctg cgccagatag cctcttacgg tcgtggttac acctatttgc tgtcacgagc
     aggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg ttgcgaagct
     gaaagagtac aacgctgcac ctccattgca gggatttggt atttccgccc cggatcaggt
     aaaagcagcg attgatgcag gagctgcggg cgcgatttct ggttcggcca ttgttaaaat
     catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag tttttgtaca
     accgatgaaa gcggcgacgc gcagttaatc ccacagccgc cagttccgct ggcggcattt
     taactttctt taatgaagcc ggaaaaatcc taaattcatt taatatttat ctttttaccg
     tttcgcttac cccggtcgat cgtggactta cgtcattttt ccgcccaaca gtaatataaa
     caaacaaatt aaacccgcaa cataacacca gtaaaatcaa taattttctc taagtcactt
     attcctcagg taattcttaa tatatccaga atgttcctca aaatatattt tccctctatc
     ttctcgttgc gcttaatttg actaattctc attagcgact aattttaatg agtgtcgagc
     agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta
     ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga
     agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc
     cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc
     tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc
     tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta
     ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc
     ctctctcgtt tcatcggtat cattaccccc atgaacagaa attccccctt acacggaggc
     atcaagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga
     cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg
     aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg
     acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aa
//