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ID   PMC1364    preliminary; circular DNA; SYN; 3926 BP.
XX
AC   IG0084;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMC1364 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pMC81 from ColE1 & lacZ gene
RC   pMC489 from pMC series
RC   pMC275 from pMC81 & pACYC177
RC   [pMC279 from pBR322 & E. coli lac operon]
RC   pMC874 from pMC275
RC   pMC511 from pMB9 & pMC489
RC   pMC871 from pMC874 & pMC511
RC   pMC1364 from pBR322 & pLL10
RC   pMC1396 from pMC1364 & pMC874
RC   pMC1388 from pMC874 & lambda RS205
RC   pMC1403 from pMC1396 & pMC1388
RC   pMC903 from pMC series
RC   pMC931 from p15A & lacZ
RA   Casadaban M.J., Chou J., Cohen S.N.;
RT   "In vitro gene fusions that join an enzymatically active
RT   beta-galactosidase segment to amino-terminal fragments of exogenous
RT   proteins: Escherichia coli plasmid vectors for the detection and
RT   cloning of translational initiation signals";
RL   J. Bacteriol. 143:971-980(1980).
XX
RN   [2]
RC   pLL10 from lambda
RA   Lau L.F., Wu R.;
RT   ;
RL   Unpublished (1980).
XX
CC   NM (pMC1364)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pLL10)
CC   BR (pMC1396)(pMC1403)(pMC1843)(pMC1844)(pMC1388)(pMC874)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pLL10 remove SmaI-EcoRI-SmaI 10bp 313..323,
FT                   \ 4313bp
FT                   -> pMC1364 3926bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   CDS             complement(2928..3788)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3926 BP; 904 A; 1076 C; 1022 G; 924 T; 0 other;
     cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     gcttcctaat gcaggagtcg cataagggag agcgtcgacc gatgcccttg agagccttca
     acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
     aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
     tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
     aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga
     cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
     ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
     gatcgctcgc ggctcttacc agcctaactt cgatcactgg accgctgatc gtcacggcga
     tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat
     accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa
     tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt
     cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc
     catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg
     catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta
     gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc
     gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg
     gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc
     ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct
     ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc
     atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac
     ccccatgaac agaaattccc ccttacacgg aggcatcaag tgaccaaaca ggaaaaaacc
     gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac
     gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag
     ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     tcacgaggcc ctttcgtctt caagaa
//