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ID   PMC1403    preliminary; circular DNA; SYN; 9820 BP.
XX
AC   IG0086;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMC1403 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pMC81 from ColE1 & lacZ gene
RC   pMC489 from pMC series
RC   pMC275 from pMC81 & pACYC177
RC   [pMC279 from pBR322 & E. coli lac operon]
RC   pMC874 from pMC275
RC   pMC511 from pMB9 & pMC489
RC   pMC871 from pMC874 & pMC511
RC   pMC1364 from pBR322 & pLL10
RC   pMC1396 from pMC1364 & pMC874
RC   pMC1388 from pMC874 & lambda RS205
RC   pMC1403 from pMC1396 & pMC1388
RC   pMC903 from pMC series
RC   pMC931 from p15A & lacZ
RA   Casadaban M.J., Chou J., Cohen S.N.;
RT   "In vitro gene fusions that join an enzymatically active
RT   beta-galactosidase segment to amino-terminal fragments of exogenous
RT   proteins: Escherichia coli plasmid vectors for the detection and
RT   cloning of translational initiation signals";
RL   J. Bacteriol. 143:971-980(1980).
XX
RN   [2]
RC   from pMC series
RA   Silhavy T.J., Beckwith J.R.;
RT   "Uses of lac fusions for the study of biological problems";
RL   Microbiological Reviews 49:398-418(1985).
XX
RN   [3]
RC   pACYC999 from ColE1 & Tn3
RC   pSC178 from pSC101 & Tn3
RC   pMC874 from pACYC177
RC   pMC931 from pMC874 & pACYC999
RC   pMC934 from pMC874 & pACYC999
RC   pMC945, pMC959 from Tn3 & pACYC999
RC   pMC995 from pMC874 & pACYC999
RC   pMC1050 from pACYC999
RC   pMC1097 from pSC178 & Tn3 R49
RC   pMC1082 from pMC874 & pMC1097
RC   pMC1102, pMC1104 from pMC1097
RC   pMC1103 from pMC1097 & pACYC999
RC   pMC1082, pMC1141, pMC1153 from pMC1097 & pACYC999
RA   Chou J., Lemaux P.G., Casadaban M.J., Cohen S.N.;
RT   "Transposition protein of Tn3: identification and characterization
RT   of an essential repressor-controlled gene product";
RL   Nature 282:801-806(1979).
XX
RN   [4]
RC   pSC105::Tn3 from pSC105 & Tn3
RC   pMC823 from pSC105::Tn3 & lacZ gene
RA   Chou J., Casadaban M.J., Lemaux P.G., Cohen S.N.;
RT   "Identification and characterization of a self-regulated repressor
RT   of translocation of the Tn3 element";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:4020-4024(1979).
XX
RN   [5]
RC   pMC81 from ColE1 & lacZ gene
RA   Casadaban M.J., Cohen S.N.;
RT   "Analysis of gene control signals by DNA fusion and cloning in
RT   Escherichia coli";
RL   J. Mol. Biol. 138:179-207(1980).
XX
CC   pMC1396 and pMC1403 are the same, except pMC1403 has lost the EcoRI
CC   site in pMC1396.
CC   NM (pMC1403)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pLL10)
CC   BR (pMC1396)(pMC1364)(pMC1843)(pMC1844)(pMC1388)(pMC874)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. ColE1 6646bp
FT                   2. E. coli, lacZ gene
FT                   -> pMC81
FT                   1. pMC81
FT                   2. pACYC177 3941bp
FT                   -> pMC275
FT                   1. pMC275
FT                   -> pMC874 [kan, unique BamHI in lacZ]
FT                   1. pMC874
FT                   recombination
FT                   2. lambda RS205, lac with mutated EcoRI
FT                   -> pMC1388
FT                   1. pMC1396 EcoRI-EcoRI 3008bp 3640..6648,
FT                   \ pLL10 lacZ region
FT                   2. pMC1388 EcoRI-EcoRI 6812bp, lacZ region of pLL10
FT                   -> pMC1403 9820bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2643..3503)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             3724..6773
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   CDS             6825..8078
FT                   /note="GEN E. coli beta-galactosidase lacY gene"
FT   CDS             8142..8753
FT                   /note="GEN E. coli beta-galactosidase lacA gene"
XX
SQ   Sequence 9820 BP; 2299 A; 2631 C; 2568 G; 2322 T; 0 other;
     ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
     tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
     tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
     atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
     ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
     ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
     atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
     ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
     ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
     catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attcccgggg atcccgtcgt
     tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc
     ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg
     gcagatgcac ggttacgatg cgcccatcta caccaacgta acctatccca ttacggtcaa
     tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga
     tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt
     tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga
     atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg
     ttggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg
     tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg
     ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt
     gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg
     caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac
     actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg
     tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt
     cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct
     gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca
     gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc
     gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt
     ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc
     gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca
     cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc
     gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg
     agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca
     gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac
     gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc
     taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg
     ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg
     tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga
     ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg
     tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga
     gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct
     ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag
     cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga
     agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct
     ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga
     gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat
     tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga
     taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg
     gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact
     tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt
     tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt
     tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca
     ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct
     tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt
     cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca
     gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac
     cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca
     tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat ttcagctgag
     cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc
     atgtctgccc gtatttcgcg taaggaaatc cattatgtac tatttaaaaa acacaaactt
     ttggatgttc ggtttattct ttttctttta cttttttatc atgggagcct acttcccgtt
     tttcccgatt tggctacatg acatcaacca tatcagcaaa agtgatacgg gtattatttt
     tgccgctatt tctctgttct cgctattatt ccaaccgctg tttggtctgc tttctgacaa
     actcgggctg cgcaaatacc tgctgtggat tattaccggc atgttagtga tgtttgcgcc
     gttctttatt tttatcttcg ggccactgtt acaatacaac attttagtag gatcgattgt
     tggtggtatt tatctaggct tttgttttaa cgccggtgcg ccagcagtag aggcatttat
     tgagaaagtc agccgtcgca gtaatttcga atttggtcgc gcgcggatgt ttggctgtgt
     tggctgggcg ctgtgtgcct cgattgtcgg catcatgttc accatcaata atcagtttgt
     tttctggctg ggctctggct gtgcactcat cctcgccgtt ttactctttt tcgccaaaac
     ggatgcgccc tcttctgcca cggttgccaa tgcggtaggt gccaaccatt cggcatttag
     ccttaagctg gcactggaac tgttcagaca gccaaaactg tggtttttgt cactgtatgt
     tattggcgtt tcctgcacct acgatgtttt tgaccaacag tttgctaatt tctttacttc
     gttctttgct accggtgaac agggtacgcg ggtatttggc tacgtaacga caatgggcga
     attacttaac gcctcgatta tgttctttgc gccactgatc attaatcgca tcggtgggaa
     aaacgccctg ctgctggctg gcactattat gtctgtacgt attattggct catcgttcgc
     cacctcagcg ctggaagtgg ttattctgaa aacgctgcat atgtttgaag taccgttcct
     gctggtgggc tgctttaaat atattaccag ccagtttgaa gtgcgttttt cagcgacgat
     ttatctggtc tgtttctgct tctttaagca actggcgatg atttttatgt ctgtactggc
     gggcaatatg tatgaaagca tcggtttcca gggcgcttat ctggtgctgg gtctggtggc
     gctgggcttc accttaattt ccgtgttcac gcttagcggc cccggcccgc tttccctgct
     gcgtcgtcag gtgaatgaag tcgcttaagc aatcaatgtc ggatgcggcg cgacgcttat
     ccgaccaaca tatcataacg gagtgatcgc attgaacatg ccaatgaccg aaagaataag
     agcaggcaag ctatttaccg atatgtgcga aggcttaccg gaaaaaagac ttcgtgggaa
     aacgttaatg tatgagttta atcactcgca tccatcagaa gttgaaaaaa gagaaagcct
     gattaaagaa atgtttgcca cggtagggga aaacgcctgg gtagaaccgc ctgtctattt
     ctcttacggt tccaacatcc atataggccg caatttttat gcaaatttca atttaaccat
     tgtcgatgac tacacggtaa caatcggtga taacgtactg attgcaccca acgttactct
     ttccgttacg ggacaccctg tacaccatga attgagaaaa aacggcgaga tgtactcttt
     tccgataacg attggcaata acgtctggat cggaagtcat gtggttatta atccaggcgt
     caccatcggg gataattctg ttattggcgc gggtagtatc gtcacaaaag acattccacc
     aaacgtcgtg gcggctggcg ttccttgtcg ggttattcgc gaaataaacg accgggataa
     gcactattat ttcaaagatt ataaagttga atcgtcagtt taaattataa aaattgcctg
     atacgctgcg cttatcaggc ctacaagttc agcgatctac attagccgca tccggcatga
     acaaagcgca ggaacaagcg tcgcatcatg cctctttgac ccacagctgc ggaaaacgta
     ctggtgcaaa acgcagggtt atgatcatca gcccaacgac gcacagcgca tgaaatgccc
     agtccatcag gtaattgccg ctgatactac gcagcacgcc agaaaaccac ggggcaagcc
     cggcgatgat aaaaccgatt ccctgcataa acgccaccag cttgccagca atagccggtt
     gcacagagtg atcgagcgcc agcagcaaac agagcggaaa cgcgccgccc agacctaacc
     cacacaccat cgcccacaat accggcaatt gcatcggcag ccagataaag ccgcagaacc
     ccaccagttg taacaccagc gccagcatta acagtttgcg ccgatcctga tggcgagcca
     tagcaggcat cagcaaagct cctgcggctt gcccaagcgt catcaatgcc agtaaggaac
     cgctgtactg cgcgctggca ccaatctcaa tatagaaagc gggtaaccag gcaatcaggc
     tggcgtaacc gccgttaatc agaccgaagt aaacacccag cgtccacgcg cggggagtga
     ataccacgcg aaccggagtg gttgttgtct tgtgggaaga ggcgacctcg cgggcgcttt
     gccaccacca ggcaaagagc gcaacaacgg caggcagcgc caccaggcga gtgtttgata
     ccaggtttcg ctatgttgaa ctaaccaggg cgttatggcg gcaccaagcc caccgccgcc
     catcagagcc gcggaccaca gccccatcac cagtggcgtg cgctgctgaa accgccgttt
     aatcaccgaa gcatcaccgc ctgaatgatg ccgatcccca ccccaccaag cagtgcgctg
     ctaagcagca gcgcactttg cgggtaaagc tcacgcatca atgcaccgac ggcaatcagc
     aacagactga tggcgacact gcgacgttcg ctgacatgct gatgaagcca gcttccggcc
     agcgccagcc cgcccatggt aaccaccggc agagcggtaa
//