Return to this vector's summary.
ID PMC1403 preliminary; circular DNA; SYN; 9820 BP.
XX
AC IG0086;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pMC1403 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pMC81 from ColE1 & lacZ gene
RC pMC489 from pMC series
RC pMC275 from pMC81 & pACYC177
RC [pMC279 from pBR322 & E. coli lac operon]
RC pMC874 from pMC275
RC pMC511 from pMB9 & pMC489
RC pMC871 from pMC874 & pMC511
RC pMC1364 from pBR322 & pLL10
RC pMC1396 from pMC1364 & pMC874
RC pMC1388 from pMC874 & lambda RS205
RC pMC1403 from pMC1396 & pMC1388
RC pMC903 from pMC series
RC pMC931 from p15A & lacZ
RA Casadaban M.J., Chou J., Cohen S.N.;
RT "In vitro gene fusions that join an enzymatically active
RT beta-galactosidase segment to amino-terminal fragments of exogenous
RT proteins: Escherichia coli plasmid vectors for the detection and
RT cloning of translational initiation signals";
RL J. Bacteriol. 143:971-980(1980).
XX
RN [2]
RC from pMC series
RA Silhavy T.J., Beckwith J.R.;
RT "Uses of lac fusions for the study of biological problems";
RL Microbiological Reviews 49:398-418(1985).
XX
RN [3]
RC pACYC999 from ColE1 & Tn3
RC pSC178 from pSC101 & Tn3
RC pMC874 from pACYC177
RC pMC931 from pMC874 & pACYC999
RC pMC934 from pMC874 & pACYC999
RC pMC945, pMC959 from Tn3 & pACYC999
RC pMC995 from pMC874 & pACYC999
RC pMC1050 from pACYC999
RC pMC1097 from pSC178 & Tn3 R49
RC pMC1082 from pMC874 & pMC1097
RC pMC1102, pMC1104 from pMC1097
RC pMC1103 from pMC1097 & pACYC999
RC pMC1082, pMC1141, pMC1153 from pMC1097 & pACYC999
RA Chou J., Lemaux P.G., Casadaban M.J., Cohen S.N.;
RT "Transposition protein of Tn3: identification and characterization
RT of an essential repressor-controlled gene product";
RL Nature 282:801-806(1979).
XX
RN [4]
RC pSC105::Tn3 from pSC105 & Tn3
RC pMC823 from pSC105::Tn3 & lacZ gene
RA Chou J., Casadaban M.J., Lemaux P.G., Cohen S.N.;
RT "Identification and characterization of a self-regulated repressor
RT of translocation of the Tn3 element";
RL Proc. Natl. Acad. Sci. U.S.A. 76:4020-4024(1979).
XX
RN [5]
RC pMC81 from ColE1 & lacZ gene
RA Casadaban M.J., Cohen S.N.;
RT "Analysis of gene control signals by DNA fusion and cloning in
RT Escherichia coli";
RL J. Mol. Biol. 138:179-207(1980).
XX
CC pMC1396 and pMC1403 are the same, except pMC1403 has lost the EcoRI
CC site in pMC1396.
CC NM (pMC1403)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE (color blue/white)
CC PA (pLL10)
CC BR (pMC1396)(pMC1364)(pMC1843)(pMC1844)(pMC1388)(pMC874)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. ColE1 6646bp
FT 2. E. coli, lacZ gene
FT -> pMC81
FT 1. pMC81
FT 2. pACYC177 3941bp
FT -> pMC275
FT 1. pMC275
FT -> pMC874 [kan, unique BamHI in lacZ]
FT 1. pMC874
FT recombination
FT 2. lambda RS205, lac with mutated EcoRI
FT -> pMC1388
FT 1. pMC1396 EcoRI-EcoRI 3008bp 3640..6648,
FT \ pLL10 lacZ region
FT 2. pMC1388 EcoRI-EcoRI 6812bp, lacZ region of pLL10
FT -> pMC1403 9820bp"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(2643..3503)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 3724..6773
FT /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT CDS 6825..8078
FT /note="GEN E. coli beta-galactosidase lacY gene"
FT CDS 8142..8753
FT /note="GEN E. coli beta-galactosidase lacA gene"
XX
SQ Sequence 9820 BP; 2299 A; 2631 C; 2568 G; 2322 T; 0 other;
ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc
aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attcccgggg atcccgtcgt
tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc
ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg
gcagatgcac ggttacgatg cgcccatcta caccaacgta acctatccca ttacggtcaa
tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga
tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt
tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga
atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg
ttggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg
tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg
ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt
gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg
caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac
actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg
tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt
cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct
gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca
gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc
gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt
ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc
gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca
cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc
gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg
agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca
gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac
gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc
taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg
ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg
tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga
ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg
tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga
gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct
ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag
cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga
agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct
ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga
gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat
tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga
taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg
gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact
tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt
tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt
tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca
ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct
tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt
cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca
gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac
cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca
tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat ttcagctgag
cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc
atgtctgccc gtatttcgcg taaggaaatc cattatgtac tatttaaaaa acacaaactt
ttggatgttc ggtttattct ttttctttta cttttttatc atgggagcct acttcccgtt
tttcccgatt tggctacatg acatcaacca tatcagcaaa agtgatacgg gtattatttt
tgccgctatt tctctgttct cgctattatt ccaaccgctg tttggtctgc tttctgacaa
actcgggctg cgcaaatacc tgctgtggat tattaccggc atgttagtga tgtttgcgcc
gttctttatt tttatcttcg ggccactgtt acaatacaac attttagtag gatcgattgt
tggtggtatt tatctaggct tttgttttaa cgccggtgcg ccagcagtag aggcatttat
tgagaaagtc agccgtcgca gtaatttcga atttggtcgc gcgcggatgt ttggctgtgt
tggctgggcg ctgtgtgcct cgattgtcgg catcatgttc accatcaata atcagtttgt
tttctggctg ggctctggct gtgcactcat cctcgccgtt ttactctttt tcgccaaaac
ggatgcgccc tcttctgcca cggttgccaa tgcggtaggt gccaaccatt cggcatttag
ccttaagctg gcactggaac tgttcagaca gccaaaactg tggtttttgt cactgtatgt
tattggcgtt tcctgcacct acgatgtttt tgaccaacag tttgctaatt tctttacttc
gttctttgct accggtgaac agggtacgcg ggtatttggc tacgtaacga caatgggcga
attacttaac gcctcgatta tgttctttgc gccactgatc attaatcgca tcggtgggaa
aaacgccctg ctgctggctg gcactattat gtctgtacgt attattggct catcgttcgc
cacctcagcg ctggaagtgg ttattctgaa aacgctgcat atgtttgaag taccgttcct
gctggtgggc tgctttaaat atattaccag ccagtttgaa gtgcgttttt cagcgacgat
ttatctggtc tgtttctgct tctttaagca actggcgatg atttttatgt ctgtactggc
gggcaatatg tatgaaagca tcggtttcca gggcgcttat ctggtgctgg gtctggtggc
gctgggcttc accttaattt ccgtgttcac gcttagcggc cccggcccgc tttccctgct
gcgtcgtcag gtgaatgaag tcgcttaagc aatcaatgtc ggatgcggcg cgacgcttat
ccgaccaaca tatcataacg gagtgatcgc attgaacatg ccaatgaccg aaagaataag
agcaggcaag ctatttaccg atatgtgcga aggcttaccg gaaaaaagac ttcgtgggaa
aacgttaatg tatgagttta atcactcgca tccatcagaa gttgaaaaaa gagaaagcct
gattaaagaa atgtttgcca cggtagggga aaacgcctgg gtagaaccgc ctgtctattt
ctcttacggt tccaacatcc atataggccg caatttttat gcaaatttca atttaaccat
tgtcgatgac tacacggtaa caatcggtga taacgtactg attgcaccca acgttactct
ttccgttacg ggacaccctg tacaccatga attgagaaaa aacggcgaga tgtactcttt
tccgataacg attggcaata acgtctggat cggaagtcat gtggttatta atccaggcgt
caccatcggg gataattctg ttattggcgc gggtagtatc gtcacaaaag acattccacc
aaacgtcgtg gcggctggcg ttccttgtcg ggttattcgc gaaataaacg accgggataa
gcactattat ttcaaagatt ataaagttga atcgtcagtt taaattataa aaattgcctg
atacgctgcg cttatcaggc ctacaagttc agcgatctac attagccgca tccggcatga
acaaagcgca ggaacaagcg tcgcatcatg cctctttgac ccacagctgc ggaaaacgta
ctggtgcaaa acgcagggtt atgatcatca gcccaacgac gcacagcgca tgaaatgccc
agtccatcag gtaattgccg ctgatactac gcagcacgcc agaaaaccac ggggcaagcc
cggcgatgat aaaaccgatt ccctgcataa acgccaccag cttgccagca atagccggtt
gcacagagtg atcgagcgcc agcagcaaac agagcggaaa cgcgccgccc agacctaacc
cacacaccat cgcccacaat accggcaatt gcatcggcag ccagataaag ccgcagaacc
ccaccagttg taacaccagc gccagcatta acagtttgcg ccgatcctga tggcgagcca
tagcaggcat cagcaaagct cctgcggctt gcccaagcgt catcaatgcc agtaaggaac
cgctgtactg cgcgctggca ccaatctcaa tatagaaagc gggtaaccag gcaatcaggc
tggcgtaacc gccgttaatc agaccgaagt aaacacccag cgtccacgcg cggggagtga
ataccacgcg aaccggagtg gttgttgtct tgtgggaaga ggcgacctcg cgggcgcttt
gccaccacca ggcaaagagc gcaacaacgg caggcagcgc caccaggcga gtgtttgata
ccaggtttcg ctatgttgaa ctaaccaggg cgttatggcg gcaccaagcc caccgccgcc
catcagagcc gcggaccaca gccccatcac cagtggcgtg cgctgctgaa accgccgttt
aatcaccgaa gcatcaccgc ctgaatgatg ccgatcccca ccccaccaag cagtgcgctg
ctaagcagca gcgcactttg cgggtaaagc tcacgcatca atgcaccgac ggcaatcagc
aacagactga tggcgacact gcgacgttcg ctgacatgct gatgaagcca gcttccggcc
agcgccagcc cgcccatggt aaccaccggc agagcggtaa
//