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ID   PMC1511    preliminary; circular DNA; SYN; 8637 BP.
XX
AC   L08935; VB0128;
XX
DT   04-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMC1511 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-8637
RC   pMC1511
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
RN   [2]
RC   pMC1511
RA   Enger-Valk B.E.;
RT   ;
RL   Submitted (21-JAN-1982) on floppy disc by:
RL   Enger-Valk B.E., .
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Revised according to sequence of pBR322 by F. Pfeiffer
CC   Inconsistent usage of mutant sequences.
CC   Pos 1237-38 is from strain UV5, while the sequences around pos
CC   1183 and around 1282 are from wild-type and not from UV5. Thus, this
CC   sequence is probably not correct.
CC   See GenBank:EcoLac for details on mutant sequences.
CC   NM (pMC1511)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(EcoLac)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. E. coli, lac promoter
FT                   -> pMC1511 8637bp"
FT   misc_feature    1..3713
FT                   /note="from pBR322"
FT   misc_feature    3716..3919
FT                   /note="from EcoLac"
FT   misc_feature    3924..4127
FT                   /note="from EcoLac"
FT   misc_feature    4142..8637
FT                   /note="from EcoLac"
FT   CDS             complement(2648..3436)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 8637 BP; 1961 A; 2276 C; 2280 G; 2120 T; 0 other;
     gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
     ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
     tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
     ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
     atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
     tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
     tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
     catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
     cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
     ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg
     cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
     aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg
     aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
     ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat
     ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
     ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt
     accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat
     cctctctcgt ttcatcggta tcattacccc catgaacaga aattccccct tacacggagg
     catcaagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag
     acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt
     gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat
     gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattccgat
     tcattaatcc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     aattaatgtg agttagctca ctcattaggc accccaggct ttacacattt atgcttccgg
     ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     atgattacgg attcactgga attccgattc attaatccag ctggcacgac aggtttcccg
     actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     cccaggcttt acacatttat gcttccggct cgtataatgt gtggaattgt gagcggataa
     caatttcaca caggaaacag ctatgaccat gattacggat tcactggaat tcccggggat
     cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
     gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc
     tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtaac ctatcccatt
     acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt
     aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac
     tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg
     ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg
     gtgctgcgtt ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc
     attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt
     gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc
     ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc
     gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat
     cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat
     ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc
     tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag
     ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg
     gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg
     cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg
     tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc
     gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat
     cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat
     cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa
     ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat
     gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct
     ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc
     ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc
     tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct
     tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc
     tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc
     cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc
     gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa
     gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag
     ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca
     tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt
     gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt
     tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag
     atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca
     ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc
     gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca
     gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa
     accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc
     gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag
     ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc
     gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc
     ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc
     ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg
     atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac
     ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattc
     cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg
     ggcaggccat gtctgcccgt atttcgcgta aggaaatcca ttatgtacta tttaaaaaac
     acaaactttt ggatgttcgg tttattcttt ttcttttact tttttatcat gggagcctac
     ttcccgtttt tcccgatttg gctacatgac atcaaccata tcagcaaaag tgatacgggt
     attatttttg ccgctatttc tctgttctcg ctattattcc aaccgctgtt tggtctgctt
     tctgacaaac tcgggctgcg caaatacctg ctgtggatta ttaccggcat gttagtgatg
     tttgcgccgt tctttatttt tatcttcggg ccactgttac aatacaacat tttagtagga
     tcgattgttg gtggtattta tctaggcttt tgttttaacg cgggtgcgcc agcagtagag
     gcatttattg agaaagtcag ccgtcgcagt aatttcgaat ttggtcgcgc gcggatgttt
     ggctgtgttg gctgggcgct gtgtgcctcg attgtcggca tcatgttcac catcaataat
     cagtttgttt tctggctggg ctctggctgt gcactcatcc tcgccgtttt actctttttc
     gccaaaacgg atgcgccctc ttctgccacg gttgccaatg cggtaggtgc caaccattcg
     gcatttagcc ttaagctggc actggaactg ttcagacagc caaaactgtg gtttttgtca
     ctgtatgtta ttggcgtttc ctgcacctac gatgtttttg accaacagtt tgctaatttc
     tttacttcgt tctttgctac cggtgaacag ggtacgcggg tatttggcta cgtaacgaca
     atgggcgaat tacttaacgc ctcgattatg ttctttgcgc cactgatcat taatcgcatc
     ggtgggaaaa acgccctgct gctggctggc actattatgt ctgtacgtat tattggctca
     tcgttcgcca cctcagcgct ggaagtggtt attctgaaaa cgctgcatat gtttgaagta
     ccgttcctgc tggtgggctg ctttaaatat attaccagcc agtttgaagt gcgtttttca
     gcgacgattt atctggtctg tttctgcttc tttaagcaac tggcgatgat ttttatgtct
     gtactggcgg gcaatatgta tgaaagcatc ggtttccagg gcgcttatct ggtgctgggt
     ctggtggcgc tgggcttcac cttaatttcc gtgttcacgc ttagcggccc cggcccgctt
     tccctgcgtc tgcgtcaggt gaatgaagtc gcttaagcaa tcaatgtcgg atgcggcgcg
     acgcttatcc gaccaacata tcataacgga gtgatcgcat tgaacatgcc aatgaccgaa
     agaataagag caggcaagct atttaccgat atgtgcgaag gcttaccgga aaaaaga
//