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ID   PMC1827    preliminary; circular DNA; SYN; 10785 BP.
XX
AC   IG9937;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMC1827 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCV1 from pBR322 & linker
RC   pSKS101 from pUC7 & Tn5
RC   pSKS114 from pUC7 & Tn9
RC   pMC1827, pMC1828 from pMC1403 & pSKS101
RC   pMC1843 from pMC1827
RC   pMLB1034 from pMC1403
RC   pMC1844 from pMLB1034 & pUC7
RC   pSKS104 from pMC1403 & pUC7
RC   pSKS105 from pMC1403 & pUC8
RC   pSKS106 from pMC1403 & pUC9
RC   pSKS107 from pSKS105
RC   pFR10 from pSKS106 & pCV1
RC   pFR97 from pSKS104 & pFR10
RC   pFR98 from pSKS105 & pFR10
RC   pFR109 from pSKS106 & pFR10
RC   plasmid from pMC279 & pMC1403
RC   plasmid2 from plasmid & pSKS101
RC   pMC1871 from plasmid2 & pMC1843
RA   Shapira S.K., Chou J., Richaud F.V., Casadaban M.J.;
RT   "New versatile plasmid vectors for expression of hybrid proteins
RT   coded by a cloned gene fused to lacZ gene sequences encoding an
RT   enzymatically active carboxy-terminal portion of beta-galactosidase";
RL   Gene 25:71-82(1983).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pMC1827)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN ()
CC   SE ()
CC   PA (pUC7)(Tn5)(pMC1403)(pSKS101)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC7 PstI 2674bp 256..256
FT                   2. Tn5 PstI-PstI 923bp 1732..2655, kan/neo gene
FT                   -> pSKS101 3597bp
FT                   1. pMC1403 EcoRI 9820bp 3640..3640
FT                   2. pSKS101 EcoRI-EcoRI 965bp, MCS/kan/neo/MCS
FT                   \ pUC7 231..256..-..256..273
FT                   -> pMC1827 10785bp"
FT   -               1..3639
FT                   /note="pMC1403 1..3639 3639bp
FT                   EcoRI = G^AATTC"
FT   -               3640..3664
FT                   /note="pUC7 231..255 25bp
FT                   PstI = CTGCA^G"
FT   -               3665..4587
FT                   /note="Tn5 1732..2654 923bp
FT                   PstI = CTGCA^G"
FT   -               4588..4604
FT                   /note="pUC7 256..272 17bp
FT                   EcoRI = G^AATTC"
FT   -               4605..10785
FT                   /note="pMC1403 3640..9820 6181bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase lacY gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase lacA gene"
XX
SQ   Sequence 10785 BP; 2472 A; 2925 C; 2863 G; 2525 T; 0 other;
     ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
     tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
     tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
     atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
     ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
     ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
     atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
     ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
     ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
     catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attccccgga tccgtcgacc
     tgcaaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     tcgcccaccc cgggctcgat cccctcgcga gttggttcag ctgctgcctg aggctggacg
     acctcgcgga gttctaccgg cagtgcaaat ccgtcggcat ccaggaaacc agcagcggct
     atccgcgcat ccatgccccc gaactgcggt cgacggatcc ggggaattcc cggggatccc
     gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg
     gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca
     aactggcaga tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg
     gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat
     gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg
     gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg
     tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg
     ctgcgttgga gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt
     ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc
     actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc
     gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc
     agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc
     gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc
     tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc
     gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg
     ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat
     gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc
     tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat
     gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat
     gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt
     aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac
     gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc
     ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa
     gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga
     gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt
     ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg
     gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac
     ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt
     gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag
     ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat
     aacgagctcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg
     cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg
     gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg
     tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg
     acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc
     atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg
     tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg
     ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa
     cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat
     acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc
     ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt
     gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg
     gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac
     cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg
     tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca
     caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg
     gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt
     ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaatttcag
     ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc
     aggccatgtc tgcccgtatt tcgcgtaagg aaatccatta tgtactattt aaaaaacaca
     aacttttgga tgttcggttt attctttttc ttttactttt ttatcatggg agcctacttc
     ccgtttttcc cgatttggct acatgacatc aaccatatca gcaaaagtga tacgggtatt
     atttttgccg ctatttctct gttctcgcta ttattccaac cgctgtttgg tctgctttct
     gacaaactcg ggctgcgcaa atacctgctg tggattatta ccggcatgtt agtgatgttt
     gcgccgttct ttatttttat cttcgggcca ctgttacaat acaacatttt agtaggatcg
     attgttggtg gtatttatct aggcttttgt tttaacgccg gtgcgccagc agtagaggca
     tttattgaga aagtcagccg tcgcagtaat ttcgaatttg gtcgcgcgcg gatgtttggc
     tgtgttggct gggcgctgtg tgcctcgatt gtcggcatca tgttcaccat caataatcag
     tttgttttct ggctgggctc tggctgtgca ctcatcctcg ccgttttact ctttttcgcc
     aaaacggatg cgccctcttc tgccacggtt gccaatgcgg taggtgccaa ccattcggca
     tttagcctta agctggcact ggaactgttc agacagccaa aactgtggtt tttgtcactg
     tatgttattg gcgtttcctg cacctacgat gtttttgacc aacagtttgc taatttcttt
     acttcgttct ttgctaccgg tgaacagggt acgcgggtat ttggctacgt aacgacaatg
     ggcgaattac ttaacgcctc gattatgttc tttgcgccac tgatcattaa tcgcatcggt
     gggaaaaacg ccctgctgct ggctggcact attatgtctg tacgtattat tggctcatcg
     ttcgccacct cagcgctgga agtggttatt ctgaaaacgc tgcatatgtt tgaagtaccg
     ttcctgctgg tgggctgctt taaatatatt accagccagt ttgaagtgcg tttttcagcg
     acgatttatc tggtctgttt ctgcttcttt aagcaactgg cgatgatttt tatgtctgta
     ctggcgggca atatgtatga aagcatcggt ttccagggcg cttatctggt gctgggtctg
     gtggcgctgg gcttcacctt aatttccgtg ttcacgctta gcggccccgg cccgctttcc
     ctgctgcgtc gtcaggtgaa tgaagtcgct taagcaatca atgtcggatg cggcgcgacg
     cttatccgac caacatatca taacggagtg atcgcattga acatgccaat gaccgaaaga
     ataagagcag gcaagctatt taccgatatg tgcgaaggct taccggaaaa aagacttcgt
     gggaaaacgt taatgtatga gtttaatcac tcgcatccat cagaagttga aaaaagagaa
     agcctgatta aagaaatgtt tgccacggta ggggaaaacg cctgggtaga accgcctgtc
     tatttctctt acggttccaa catccatata ggccgcaatt tttatgcaaa tttcaattta
     accattgtcg atgactacac ggtaacaatc ggtgataacg tactgattgc acccaacgtt
     actctttccg ttacgggaca ccctgtacac catgaattga gaaaaaacgg cgagatgtac
     tcttttccga taacgattgg caataacgtc tggatcggaa gtcatgtggt tattaatcca
     ggcgtcacca tcggggataa ttctgttatt ggcgcgggta gtatcgtcac aaaagacatt
     ccaccaaacg tcgtggcggc tggcgttcct tgtcgggtta ttcgcgaaat aaacgaccgg
     gataagcact attatttcaa agattataaa gttgaatcgt cagtttaaat tataaaaatt
     gcctgatacg ctgcgcttat caggcctaca agttcagcga tctacattag ccgcatccgg
     catgaacaaa gcgcaggaac aagcgtcgca tcatgcctct ttgacccaca gctgcggaaa
     acgtactggt gcaaaacgca gggttatgat catcagccca acgacgcaca gcgcatgaaa
     tgcccagtcc atcaggtaat tgccgctgat actacgcagc acgccagaaa accacggggc
     aagcccggcg atgataaaac cgattccctg cataaacgcc accagcttgc cagcaatagc
     cggttgcaca gagtgatcga gcgccagcag caaacagagc ggaaacgcgc cgcccagacc
     taacccacac accatcgccc acaataccgg caattgcatc ggcagccaga taaagccgca
     gaaccccacc agttgtaaca ccagcgccag cattaacagt ttgcgccgat cctgatggcg
     agccatagca ggcatcagca aagctcctgc ggcttgccca agcgtcatca atgccagtaa
     ggaaccgctg tactgcgcgc tggcaccaat ctcaatatag aaagcgggta accaggcaat
     caggctggcg taaccgccgt taatcagacc gaagtaaaca cccagcgtcc acgcgcgggg
     agtgaatacc acgcgaaccg gagtggttgt tgtcttgtgg gaagaggcga cctcgcgggc
     gctttgccac caccaggcaa agagcgcaac aacggcaggc agcgccacca ggcgagtgtt
     tgataccagg tttcgctatg ttgaactaac cagggcgtta tggcggcacc aagcccaccg
     ccgcccatca gagccgcgga ccacagcccc atcaccagtg gcgtgcgctg ctgaaaccgc
     cgtttaatca ccgaagcatc accgcctgaa tgatgccgat ccccacccca ccaagcagtg
     cgctgctaag cagcagcgca ctttgcgggt aaagctcacg catcaatgca ccgacggcaa
     tcagcaacag actgatggcg acactgcgac gttcgctgac atgctgatga agccagcttc
     cggccagcgc cagcccgccc atggtaacca ccggcagagc ggtaa
//