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ID   PMC1843    preliminary; circular DNA; SYN; 9862 BP.
XX
AC   IG0087;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMC1843 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCV1 from pBR322 & linker
RC   pSKS101 from pUC7 & Tn5
RC   pSKS114 from pUC7 & Tn9
RC   pMC1827, pMC1828 from pMC1403 & pSKS101
RC   pMC1843 from pMC1827
RC   pMLB1034 from pMC1403
RC   pMC1844 from pMLB1034 & pUC7
RC   pSKS104 from pMC1403 & pUC7
RC   pSKS105 from pMC1403 & pUC8
RC   pSKS106 from pMC1403 & pUC9
RC   pSKS107 from pSKS105
RC   pFR10 from pSKS106 & pCV1
RC   pFR97 from pSKS104 & pFR10
RC   pFR98 from pSKS105 & pFR10
RC   pFR109 from pSKS106 & pFR10
RC   plasmid from pMC279 & pMC1403
RC   plasmid2 from plasmid & pSKS101
RC   pMC1871 from plasmid2 & pMC1843
RA   Shapira S.K., Chou J., Richaud F.V., Casadaban M.J.;
RT   "New versatile plasmid vectors for expression of hybrid proteins
RT   coded by a cloned gene fused to lacZ gene sequences encoding an
RT   enzymatically active carboxy-terminal portion of beta-galactosidase";
RL   Gene 25:71-82(1983).
XX
CC   pMC1843 lacks the lac promoter, operator, RBS, translational ATG,
CC   and the first seven codons of lacZ.
CC   NM (pMC1843)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (promoter analysis)
CC   SE (color blue/white)
CC   PA (pMC1403)
CC   BR (pMC1844)(pMLB1034)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC7 PstI 2674bp 256..256
FT                   2. Tn5 PstI-PstI 923bp 1732..2655, kan/neo gene
FT                   -> pSKS101 3597bp
FT                   1. pMC1403 EcoRI 9820bp 3640..3640
FT                   2. pSKS101 EcoRI-EcoRI 965bp, MCS/kan/neo/MCS
FT                   \ pUC7 231..256..-..256..273
FT                   -> pMC1827 10785bp
FT                   1. pMC1827 remove PstI-PstI 923bp, kan/neo gene
FT                   \ Tn5 1732..2655
FT                   -> pMC1843 9862bp [PstI, SalI]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2643..3503)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             3768..6816
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   CDS             6867..8121
FT                   /note="GEN E. coli beta-galactosidase lacY gene"
FT   CDS             8184..8796
FT                   /note="GEN E. coli beta-galactosidase lacA gene"
XX
SQ   Sequence 9862 BP; 2306 A; 2647 C; 2581 G; 2328 T; 0 other;
     ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
     tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
     tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
     atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
     ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
     ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
     atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
     ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
     ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
     catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attccccgga tccgtcgacc
     tgcaggtcga cggatccggg gaattccccg ggatcccgtc gttttacaac gtcgtgactg
     ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg
     cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga
     tgcgcccatc tacaccaacg taacctatcc cattacggtc aatccgccgt ttgttcccac
     ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga
     aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg
     gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt
     tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgttggagtg acggcagtta
     tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca
     taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag
     ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt
     aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg
     tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga
     aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca
     caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg
     gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg
     tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat
     cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc
     gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga
     aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat
     gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg
     gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa
     atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac
     cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc
     gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg
     cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt
     tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat
     taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc
     gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc
     gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat
     cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt
     ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg
     taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct
     cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc
     ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc
     catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg
     gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact
     gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag
     tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta
     ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat
     tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta
     ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac
     accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa
     ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga
     ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg
     tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca
     gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct
     gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga
     cgactcctgg agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta
     ccagttggtc tggtgtcaaa aataataata accgggcagg ccatgtctgc ccgtatttcg
     cgtaaggaaa tccattatgt actatttaaa aaacacaaac ttttggatgt tcggtttatt
     ctttttcttt tactttttta tcatgggagc ctacttcccg tttttcccga tttggctaca
     tgacatcaac catatcagca aaagtgatac gggtattatt tttgccgcta tttctctgtt
     ctcgctatta ttccaaccgc tgtttggtct gctttctgac aaactcgggc tgcgcaaata
     cctgctgtgg attattaccg gcatgttagt gatgtttgcg ccgttcttta tttttatctt
     cgggccactg ttacaataca acattttagt aggatcgatt gttggtggta tttatctagg
     cttttgtttt aacgccggtg cgccagcagt agaggcattt attgagaaag tcagccgtcg
     cagtaatttc gaatttggtc gcgcgcggat gtttggctgt gttggctggg cgctgtgtgc
     ctcgattgtc ggcatcatgt tcaccatcaa taatcagttt gttttctggc tgggctctgg
     ctgtgcactc atcctcgccg ttttactctt tttcgccaaa acggatgcgc cctcttctgc
     cacggttgcc aatgcggtag gtgccaacca ttcggcattt agccttaagc tggcactgga
     actgttcaga cagccaaaac tgtggttttt gtcactgtat gttattggcg tttcctgcac
     ctacgatgtt tttgaccaac agtttgctaa tttctttact tcgttctttg ctaccggtga
     acagggtacg cgggtatttg gctacgtaac gacaatgggc gaattactta acgcctcgat
     tatgttcttt gcgccactga tcattaatcg catcggtggg aaaaacgccc tgctgctggc
     tggcactatt atgtctgtac gtattattgg ctcatcgttc gccacctcag cgctggaagt
     ggttattctg aaaacgctgc atatgtttga agtaccgttc ctgctggtgg gctgctttaa
     atatattacc agccagtttg aagtgcgttt ttcagcgacg atttatctgg tctgtttctg
     cttctttaag caactggcga tgatttttat gtctgtactg gcgggcaata tgtatgaaag
     catcggtttc cagggcgctt atctggtgct gggtctggtg gcgctgggct tcaccttaat
     ttccgtgttc acgcttagcg gccccggccc gctttccctg ctgcgtcgtc aggtgaatga
     agtcgcttaa gcaatcaatg tcggatgcgg cgcgacgctt atccgaccaa catatcataa
     cggagtgatc gcattgaaca tgccaatgac cgaaagaata agagcaggca agctatttac
     cgatatgtgc gaaggcttac cggaaaaaag acttcgtggg aaaacgttaa tgtatgagtt
     taatcactcg catccatcag aagttgaaaa aagagaaagc ctgattaaag aaatgtttgc
     cacggtaggg gaaaacgcct gggtagaacc gcctgtctat ttctcttacg gttccaacat
     ccatataggc cgcaattttt atgcaaattt caatttaacc attgtcgatg actacacggt
     aacaatcggt gataacgtac tgattgcacc caacgttact ctttccgtta cgggacaccc
     tgtacaccat gaattgagaa aaaacggcga gatgtactct tttccgataa cgattggcaa
     taacgtctgg atcggaagtc atgtggttat taatccaggc gtcaccatcg gggataattc
     tgttattggc gcgggtagta tcgtcacaaa agacattcca ccaaacgtcg tggcggctgg
     cgttccttgt cgggttattc gcgaaataaa cgaccgggat aagcactatt atttcaaaga
     ttataaagtt gaatcgtcag tttaaattat aaaaattgcc tgatacgctg cgcttatcag
     gcctacaagt tcagcgatct acattagccg catccggcat gaacaaagcg caggaacaag
     cgtcgcatca tgcctctttg acccacagct gcggaaaacg tactggtgca aaacgcaggg
     ttatgatcat cagcccaacg acgcacagcg catgaaatgc ccagtccatc aggtaattgc
     cgctgatact acgcagcacg ccagaaaacc acggggcaag cccggcgatg ataaaaccga
     ttccctgcat aaacgccacc agcttgccag caatagccgg ttgcacagag tgatcgagcg
     ccagcagcaa acagagcgga aacgcgccgc ccagacctaa cccacacacc atcgcccaca
     ataccggcaa ttgcatcggc agccagataa agccgcagaa ccccaccagt tgtaacacca
     gcgccagcat taacagtttg cgccgatcct gatggcgagc catagcaggc atcagcaaag
     ctcctgcggc ttgcccaagc gtcatcaatg ccagtaagga accgctgtac tgcgcgctgg
     caccaatctc aatatagaaa gcgggtaacc aggcaatcag gctggcgtaa ccgccgttaa
     tcagaccgaa gtaaacaccc agcgtccacg cgcggggagt gaataccacg cgaaccggag
     tggttgttgt cttgtgggaa gaggcgacct cgcgggcgct ttgccaccac caggcaaaga
     gcgcaacaac ggcaggcagc gccaccaggc gagtgtttga taccaggttt cgctatgttg
     aactaaccag ggcgttatgg cggcaccaag cccaccgccg cccatcagag ccgcggacca
     cagccccatc accagtggcg tgcgctgctg aaaccgccgt ttaatcaccg aagcatcacc
     gcctgaatga tgccgatccc caccccacca agcagtgcgc tgctaagcag cagcgcactt
     tgcgggtaaa gctcacgcat caatgcaccg acggcaatca gcaacagact gatggcgaca
     ctgcgacgtt cgctgacatg ctgatgaagc cagcttccgg ccagcgccag cccgcccatg
     gtaaccaccg gcagagcggt aa
//