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ID   PMC1844    preliminary; circular DNA; SYN; 6230 BP.
XX
AC   IG0088; K01450;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMC1844 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCV1 from pBR322 & linker
RC   pSKS101 from pUC7 & Tn5
RC   pSKS114 from pUC7 & Tn9
RC   pMC1827, pMC1828 from pMC1403 & pSKS101
RC   pMC1843 from pMC1827
RC   pMLB1034 from pMC1403
RC   pMC1844 from pMLB1034 & pUC7
RC   pSKS104 from pMC1403 & pUC7
RC   pSKS105 from pMC1403 & pUC8
RC   pSKS106 from pMC1403 & pUC9
RC   pSKS107 from pSKS105
RC   pFR10 from pSKS106 & pCV1
RC   pFR97 from pSKS104 & pFR10
RC   pFR98 from pSKS105 & pFR10
RC   pFR109 from pSKS106 & pFR10
RC   plasmid from pMC279 & pMC1403
RC   plasmid2 from plasmid & pSKS101
RC   pMC1871 from plasmid2 & pMC1843
RA   Shapira S.K., Chou J., Richaud F.V., Casadaban M.J.;
RT   "New versatile plasmid vectors for expression of hybrid proteins
RT   coded by a cloned gene fused to lacZ gene sequences encoding an
RT   enzymatically active carboxy-terminal portion of beta-galactosidase";
RL   Gene 25:71-82(1983).
XX
CC   pMC1844 lacks the lac promoter, operator, RBS, translational ATG,
CC   and the first seven codons of LacZ.
CC   NM (pMC1844)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (promoter analysis)
CC   SE (color blue/white)
CC   PA (pMC1403)
CC   BR (pMC1843)(pMLB1034)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC7 PstI 2674bp 256..256
FT                   2. Tn5 PstI-PstI 923bp 1732..2655, kan/neo gene
FT                   -> pSKS101 3597bp
FT                   1. pMLB1034 EcoRI 6189bp 2867..2867
FT                   2. pSKS101 EcoRI-EcoRI 965bp, MCS/kan/neo/MCS
FT                   \ pUC7 231..256..-..256..273
FT                   -> plasmid 7154bp
FT                   2. plasmid remove PstI-PstI 923bp, kan/neo gene
FT                   \ Tn5 1732..2655
FT                   -> pMC1844 6230bp [unique SalI]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2643..3503)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             3766..6815
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
XX
SQ   Sequence 6230 BP; 1466 A; 1656 C; 1642 G; 1466 T; 0 other;
     gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga
     ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt
     ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat
     cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca
     ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc
     acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc
     tcgtttcatc ggtatcatta cccccatgaa cagaaattcc cccttacacg gaggcatcaa
     gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta
     acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg
     cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt
     gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaattc cccggatccg
     tcgacctgca ggtcgacgga tccggggaat tccccgggat cccgtcgttt tacaacgtcg
     tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct
     ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg
     ttacgatgcg cccatctaca ccaacgtaac ctatcccatt acggtcaatc cgccgtttgt
     tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct
     acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc atctgtggtg
     caacgggcgc tgggtcggtt acggccagga cagtcgtttg ccgtctgaat ttgacctgag
     cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgtt ggagtgacgg
     cagttatctg gaagatcagg atatgtggcg gatgagcggc attttccgtg acgtctcgtt
     gctgcataaa ccgactacac aaatcagcga tttccatgtt gccactcgct ttaatgatga
     tttcagccgc gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc gtgactacct
     acgggtaaca gtttctttat ggcagggtga aacgcaggtc gccagcggca ccgcgccttt
     cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa
     cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg cggtggttga
     actgcacacc gccgacggca cgctgattga agcagaagcc tgcgatgtcg gtttccgcga
     ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt
     taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca
     ggatatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc attatccgaa
     ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa
     tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc gctggctacc
     ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat
     catctggtcg ctggggaatg aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg
     gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag ccgacaccac
     ggccaccgat attatttgcc cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc
     tgtgccgaaa tggtccatca aaaaatggct ttcgctacct ggagagacgc gcccgctgat
     cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta aatactggca
     ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc tgggactggg tggatcagtc
     gctgattaaa tatgatgaaa acggcaaccc gtggtcggct tacggcggtg attttggcga
     tacgccgaac gatcgccagt tctgtatgaa cggtctggtc tttgccgacc gcacgccgca
     tccagcgctg acggaagcaa aacaccagca gcagtttttc cagttccgtt tatccgggca
     aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc gataacgagc tcctgcactg
     gatggtggcg ctggatggta agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc
     acaaggtaaa cagttgattg aactgcctga actaccgcag ccggagagcg ccgggcaact
     ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat
     cagcgcctgg cagcagtggc gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc
     ccacgccatc ccgcatctga ccaccagcga aatggatttt tgcatcgagc tgggtaataa
     gcgttggcaa tttaaccgcc agtcaggctt tctttcacag atgtggattg gcgataaaaa
     acaactgctg acgccgctgc gcgatcagtt cacccgtgca ccgctggata acgacattgg
     cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg
     ccattaccag gccgaagcag cgttgttgca gtgcacggca gatacacttg ctgatgcggt
     gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta tcagccggaa
     aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg aagtggcgag
     cgatacaccg catccggcgc ggattggcct gaactgccag ctggcgcagg tagcagagcg
     ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta ctgccgcctg
     ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct tcccgagcga
     aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga
     cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca gccatcgcca
     tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata tggggattgg
     tggcgacgac tcctggagcc cgtcagtatc ggcggaattc cagctgagcg ccggtcgcta
     ccattaccag ttggtctggt gtcaaaaata ataataaccg ggcaggccat gtctgcccgt
     atttcgcgta aggaaatcca ttatgtacta tttaaaaaac acaaactttt ggatgttcgg
     tttattcttt ttcttttact tttttatcat gggagcctac ttcccgtttt tcccgatttg
     gctacatgac atcaaccata tcagcaaaag tgatacgggt attatttttg ccgctatttc
     tctgttctcg ctattattcc aaccgctgtt tggtctgctt tctgacaaac
//