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ID   PMC1871    preliminary; circular DNA; SYN; 7476 BP.
XX
AC   L08936; VB0127; IG1151; K01450;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMC1871 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCV1 from pBR322 & linker
RC   pSKS101 from pUC7 & Tn5
RC   pSKS114 from pUC7 & Tn9
RC   pMC1827, pMC1828 from pMC1403 & pSKS101
RC   pMC1843 from pMC1827
RC   pMLB1034 from pMC1403
RC   pMC1844 from pMLB1034 & pUC7
RC   pSKS104 from pMC1403 & pUC7
RC   pSKS105 from pMC1403 & pUC8
RC   pSKS106 from pMC1403 & pUC9
RC   pSKS107 from pSKS105
RC   pFR10 from pSKS106 & pCV1
RC   pFR97 from pSKS104 & pFR10
RC   pFR98 from pSKS105 & pFR10
RC   pFR109 from pSKS106 & pFR10
RC   plasmid from pMC279 & pMC1403
RC   plasmid2 from plasmid & pSKS101
RC   pMC1871 from plasmid2 & pMC1843
RA   Shapira S.K., Chou J., Richaud F.V., Casadaban M.J.;
RT   "New versatile plasmid vectors for expression of hybrid proteins
RT   coded by a cloned gene fused to lacZ gene sequences encoding an
RT   enzymatically active carboxy-terminal portion of beta-galactosidase";
RL   Gene 25:71-82(1983).
XX
RN   [2]
RC   pMC1513 from pMC1403 & pKB252, lacUV5 promoter
RC   pSKS104 from pMC1403 & M13mp7
RC   pSKS105 from pMC1403 & M13mp8
RC   pSKS106 from pMC1403 & M13mp9
RC   pMC1585 from pMC1403 & yeast 2 micron & URA3 gene
RC   pMC1587 from pMC1403 & yeast 2 micron & LEU2 gene
RC   pMC1790 from pMC1403 & ARS1 & TRP1 gene
RC   pMC2010 from pMC1403 & yeast 2 micron & TRP1 gene
RC   pMC931 from pMC1403 & pACYC177
RC   pMC1871 from pMC1403 & M13mp7
RC   [pMC1364 from pMC series]
RC   [pMC1396 from pMC series]
RC   [pMC1511 from pMC series]
RC   [pMC1571 from pMC1511]
RC   [pMC1981 from pMC1871]
RA   Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J.;
RT   "Beta-galactosidase gene fusions for analyzing gene expression in
RT   Escherichia coli and yeast";
RL   Meth. Enzymol. 100:293-308(1983).
XX
RN   [3]
RC   pDN9 from pMC1403 & lambda rI2 or lambda rI22
RC   pSV2beta-globin from pSV2
RC   pMC1807 from pMC931 & pSV2beta-globin
RC   pMC1924 from pMC1871 & pMC1807
RC   pDN26 from pDN9 & pMC1924 & pBR322
RA   Nielsen D.A., Chou J., MacKrell A.J., Casadaban M.J., Steiner D.F.;
RT   "Expression of a preproinsulin-beta-galactosidase gene fusion in
RT   mammalian cells";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:5198-5202(1983).
XX
RN   [4]
RP   1-7478
RC   pMC1871
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
RN   [5]
RC   lambda KB1 from lambda & lac promoter
RC   pKB265 from pKB252
RC   pKB268 from pMB9 & lacUV5 promoter & lambda, cI gene
RC   pKB280 from pMB9 & lacUV5 promoter & lambda, cI gene
RA   Backman K., Ptashne M.;
RT   "Maximizing gene expression on a plasmid using recombination in
RT   vitro";
RL   Cell 13:65-71(1978).
XX
RN   [6]
RC   lambda rI1 or lambda rI1 from Charon 4A & rat preproinsulin gene
RC   lambda rI2 or lambda rI2 from Charon 4A & rat preproinsulin gene
RC   lambda rI22 or lambda rI22 from Charon 4A & rat preproinsulin gene
RA   Lomedico P., Rosenthal N., Efstratiadis A., Gilbert W., Kolodner R.,
RA   Tizard R.;
RT   "The structure and evolution of the two nonallelic rat
RT   preproinsulin genes";
RL   Cell 18:545-558(1979).
XX
RN   [7]
RC   pKB166 from pKB252
RA   Backman K.;
RT   "Molecular cloning of the lambda repressor gene (cI)";
RL   Thesis [Harvard University] 0:0-0(1977).
XX
RN   [8]
RC   pMC1871
RA   Enger-Valk B.E.;
RT   ;
RL   Submitted (00-XXX-1985) on floppy disc by:
RL   Enger-Valk B.E., .
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Revised according to sequence of pBR322 by F. Pfeiffer
CC   The ampicillin coding region has been separated from the
CC   promoter. Thus
CC   this vector does not confer ampicillin resistance.
CC   The polylinker sites are not unique in this vector!
CC   The GenBank entry is not current with the Pharmacia entry (1993).
CC   NM (pMC1871)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli JM105)
CC   CP ()
CC   FN (expression)
CC   SE (color blue/white)(affinity)
CC   PA (pBR322)(pMC1843)(pMC1826)(EcoLac)
CC   BR (pMC1843)(pMC1826)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. E. coli, lac operon
FT                   -> pMC279
FT                   1. pMC279 remove SmaI-HindIII, 3' lacZ gene
FT                   \ pBR322 HindIII 30/SmaI in lac
FT                   -> pMC1764
FT                   1. pMC1764 remove small EcoRI-BamHI, 3' lacZ gene
FT                   2. pMC1403 EcoRI-BamHI 10bp 3640..3650,
FT                   \ MCS EcoRI-SmaI-BamHI
FT                   -> pMC1788 [unique SmaI]
FT                   1. pMC1788 remove small EcoRI-SmaI 3640..3647, MCS
FT                   2. pMC279 HaeIII-HaeIII 247bp, 3' lacZ gene
FT                   EcoRI-HaeIII
FT                   -> pMC1817
FT                   1. pUC7 PstI 2674bp 256..256
FT                   2. Tn5 PstI-PstI 923bp 1732..2655 , kan/neo gene
FT                   -> pSKS101 3597bp
FT                   1. pMC1817 BamHI, MCS
FT                   2. pSKS101 BamHI-BamHI 947bp, kan/neo
FT                   \ pUC7 240..256..-..256..264
FT                   -> pMC1826
FT                   1. pMC1826 PstI-SacI 1154bp 2892..-..3665, lacZ 3'
FT                   \ pMC1403 PstI = 2892 3665/pMC1403 SacI = 5626
FT                   2. pMC1843 PstI-SacI 1961bp 3665..5626, lacZ gene 5'
FT                   3. pBR322 PstI 4361bp 3612..3612, amp gene
FT                   -> pMC1871 7476bp"
FT   misc_binding    1..32
FT                   /note="MCS PstI-SalI-BamHI-EcoRI-SmaI-BamHI"
FT   misc_binding    27..27
FT                   /note="SIT unique SmaI"
FT   CDS             33..3103
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide, promoterless"
FT   misc_binding    1962..1962
FT                   /note="SIT unique SacI"
FT   misc_binding    3030..3030
FT                   /note="SIT EcoRI"
FT   misc_binding    3104..3116
FT                   /note="MCS BamHI-SalI-PstI"
FT   CDS             >3868..<5500
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    1..3615
FT                   /note="from pBR322"
FT   misc_feature    3609..3645
FT                   /note="MCS polylinker region I"
FT   misc_feature    3645..6709
FT                   /note="from EcoLac"
FT   misc_feature    6712..6729
FT                   /note="MCS polylinker region II"
FT   misc_feature    6724..7478
FT                   /note="from pBR322"
XX
SQ   Sequence 7476 BP; 1668 A; 2063 C; 2040 G; 1705 T; 0 other;
     ctgcaggtcg acggatccgg ggaattcccg gggatcccgt cgttttacaa cgtcgtgact
     gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt
     gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg
     atgcgcccat ctacaccaac gtaacctatc ccattacggt caatccgccg tttgttccca
     cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg
     aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg tggtgcaacg
     ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac ctgagcgcat
     ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgttggagt gacggcagtt
     atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc
     ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat gatgatttca
     gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac tacctacggg
     taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg
     gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg
     aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc
     acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc
     ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc
     gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg gtgcaggata
     tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc
     cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa gccaatattg
     aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga
     tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct
     ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca
     aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca
     ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc
     cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgatccttt
     gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt
     ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga
     ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt ggcgatacgc
     cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg ccgcatccag
     cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc gggcaaacca
     tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg cactggatgg
     tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag
     gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg caactctggc
     tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg cacatcagcg
     cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg
     ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt aataagcgtt
     ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat aaaaaacaac
     tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac attggcgtaa
     gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt
     accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat gcggtgctga
     ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc cggaaaacct
     accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg gcgagcgata
     caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca gagcgggtaa
     actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg
     accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg agcgaaaacg
     gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc ggcgacttcc
     agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat cgccatctgc
     tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg attggtggcg
     acgactcctg gagcccgtca gtatcggcgg aattccagct gagcgccggt cgctaccatt
     accagttggt ctggtgtcaa aaataataat aaccgggcag gggggatccg tcgacctgca
     ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
     cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     cgtatcacga ggccctttcg tcttcaagaa ttctcatgtt tgacagctta tcatcgataa
     gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa
     atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
     cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
     cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt
     tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg
     agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg
     acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
     cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
     ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
     tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
     cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
     ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc
     tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc
     ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct
     ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg
     cacctcgcta acggattcac cactccaaga attggagcca atcaattctt gcggagaact
     gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc
     cgcacgcggc gcatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc
     ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca
     ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca
     acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc
     tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct
     acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc
     atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca
     gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga
     aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc
     cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat
     gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc
     ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     cgccagttaa tagtttgcgc aacgttgttg ccattg
//