Return to this vector's summary.
ID PMDSG preliminary; circular DNA; SYN; 7626 BP.
XX
AC IG0091;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pMDSG - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pSV2-dhfr from SV40 & pBR322 & mouse DHFR gene
RC pSV0-dhfr from pSV2-dhfr
RC pSVMdhfr from pSV2-dhfr & MMTV
RC pMTVdhfr from pSVMdhfr
RC pMSG or pMDSG from pMTVdhfr & E.coli gpt gene
RA Lee F., Mulligan R.C., Berg P., Ringold G.M.;
RT "Glucocorticoids regulate expression of dihydrofolate reductase
RT cDNA in mouse mammary tumour virus chimaeric plasmids";
RL Nature 294:228-232(1981).
XX
RN [2]
RC pSVMgpt from pSV2-gpt & MMTV LTR
RA Chapman A.B., Costello M.A., Lee F., Ringold G.M.;
RT "Amplification and hormone-regulated expression of a mouse mammary
RT tumor virus-Eco gpt fusion plasmid in mouse 3T6 cells";
RL Mol. Cell. Biol. 3:1421-1429(1983).
XX
RN [3]
RC pLC2 from MMTV LTR & pLC1
RC pGTC1 from MMTV LTR & pLC1
RC pMC1 from MMTV LTR & pLC1
RC pMC2 from pLC1
RC pGMC1.5A/B, pGMC1.3A/B from pMC1 & GRE
RC pNMC1 from pMC1 & linker
RC pNMC2, pNMC3 from pMC1 & linker
RA Morley K.L., Toohey M.G., Peterson D.O.;
RT "Transcriptional repression of a hormone-responsive promoter";
RL Nucleic Acids Res. 15:6973-6989(1987).
XX
RN [4]
RC pSV01 from pBR322 & SV40 ori & linker
RC pSV0d from pML-RIIG & pSV01
RC pSV0alpha series from pSV0d & human alpha-globin gene
RA Mellon P., Parker V., Gluzman Y., Maniatis T.;
RT "Identification of DNA sequences required for transcription of the
RT human alpha 1-globin gene in a new SV40 host-vector system";
RL Cell 27:279-288(1981).
XX
RN [5]
RC plasmid from pSV0d & linker
RC pMT1 from plasmid & pZ152
RC plasmid2 from pMT1 & pSV2-cat
RC pLC1 from plasmid2 & MMTV LTR
RC pLC2 from pLC1 & pUC18
RC pLCL1 from pLC1 & MMTV LTR
RC p23LC1 from pMTV1 & pLC1
RA Toohey M.G., Morley K.L., Peterson D.O.;
RT "Multiple hormone-inducible enhancers as mediators of differential
RT transcription";
RL Mol. Cell. Biol. 6:4526-4538(1986).
XX
RN [6]
RC pMTV1 from MMTV
RA Ucker D.S., Ross S.R., Yamamoto K.R.;
RT "Mammary tumor virus DNA contains sequences required for its
RT hormone regulated transcription";
RL Cell 27:257-266(1981).
XX
CC mouse mammary tumor virus long terminal repeat is a glucocorticoid
CC -inducible promoter, e.g., by dexamethasone.
CC uses first ATG in inserted DNA.
CC NM (pMDSG)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Pharmacia)
CC HO (E.coli HB101)(hormonally responsive mammal)
CC HO (Chinese hamster ovary CHO)(human HeLa)(mouse Ltk-)(mouse 3T6)
CC CP ()
CC FN (expression shuttle)(expression inducible)
CC SE ()
CC PA (pBR322)
CC BR ()
CC OF (pMSG-CAT)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322, ori/amp gene
FT 2. SV40, ori/early splice/polyA
FT 3. mouse, DHFR gene
FT -> pSV2-dhfr [unique HindIII]
FT 1. pSV2-dhfr
FT -> pSV0-dhfr
FT 1. pSV2-dhfr HindIII
FT 2. MMTV PstI-PstI 1450bp, MMTV LTR
FT T4 DNA polymerase:T4 DNA polymerase
FT HindIII linker 6bp aagctt:HindIII linker 6bp aagctt
FT HindIII-HindIII
FT -> pSVMdhfr
FT 1. pSV2-dhfr remove PvuII-HindIII, SV40 early promoter
FT HindIII linker 6bp aagctt:
FT 2. MMTV PstI-PstI 1450bp, MMTV LTR
FT T4 DNA polymerase:T4 DNA polymerase
FT HindIII linker 6bp aagctt:HindIII linker 6bp aagctt
FT HindIII-HindIII
FT -> pMTVdhfr
FT 1. pMTVdhfr BamHI
FT 2. pSV2-gpt PvuII-BamHI 2200bp, SV40 ori/early/gpt
FT BamHI linker 6bp ggatcc:
FT -> pMDSG 7626bp"
FT misc_binding 1..23
FT /note="MCS unique NheI-SmaI-SalI-XhoI"
FT misc_feature >23..<877
FT /note="SPL SV40 early genes"
FT polyA_signal >23..<877
FT /note="PLA SV40 early genes"
FT rep_origin 871..1217
FT /note="ORI SV40"
FT promoter 871..1217
FT /note="PRO SV40 early genes"
FT CDS 1217..<2988
FT /note="GEN E. coli xanthine-guanine
FT phosphoribosyl-transferase (gpt) gene"
FT misc_binding 1237..1237
FT /note="SIT unique BglII"
FT misc_binding 1535..1535
FT /note="SIT unique KpnI"
FT misc_feature 0..0
FT /note="SPL SV40 early genes"
FT polyA_signal 0..0
FT /note="PLA SV40 early genes"
FT misc_binding 3872..3872
FT /note="SIT unique EcoRI"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 4498..4498
FT /note="SIT unique PvuI"
FT rep_origin >4700..<6011
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_binding 6011..6011
FT /note="SIT unique Tth111I"
FT misc_binding 6171..6171
FT /note="SIT HindIII"
FT promoter 6171..7626
FT /note="PRO mouse mammary tumor virus (MMTV)
FT long terminal repeat (LTR), PstI-PstI fragment"
FT misc_binding 6498..6498
FT /note="SIT unique ClaI"
FT misc_binding 7251..7251
FT /note="SIT unique SacI"
XX
SQ Sequence 7626 BP; 2081 A; 1735 C; 1694 G; 2116 T; 0 other;
gctagcccgg ggtcgacctc gagggatctt tgtgaaggaa ccttacttct gtggtgtgac
ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
ccaaactcat caatgtatct tatcatgtct ggatccgctg tggaatgtgt gtcagttagg
gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
ggaaaggcat tattgccgta agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg
aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt
tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
ggatccccag gaagctcctc tgtgtcctca taaaccctaa cctcctctac ttgagaggac
attccaatca taggctgccc atccaccctc tgtgtcctcc tgttaattag gtcacttaac
aaaaaggaaa ttgggtaggg gtttttcaca gaccgctttc taagggtaat tttaaaatat
ctgggaagtc ccttccactg ctgtgttcca gaagtgttgg taaacagccc acaaatgtca
acagcagaaa catacaagct gtcagctttg cacaagggcc caacaccctg ctcatcaaga
agcactgtgg ttgctgtgtt agtaatgtgc aaaacaggag gcacattttc cccacctgtg
taggttccaa aatatctagt gttttcattt ttacttggat caggaaccca gcactccact
ggataagcat tatccttatc caaaacagcc ttgtggtcag tgttcatctg ctgactgtca
actgtagcat tttttggggt tacagtttga gcaggatatt tggtcctgta gtttgctaac
acaccctgca gctccaaagg ttccccacca acagcaaaaa aatgaaaatt tgacccttga
atgggttttc cagcaccatt ttcatgagtt ttttgtgtcc ctgaatgcaa gtttaacata
gcagttaccc caataacctc agttttaaca gtaacagctt cccacatcaa aatatttcca
caggttaagt cctcatttaa attaggcaaa ggaattcttg aagacgaaag ggcctcgtga
tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc aagcttggca
gaaatggttg aactcccgag agtgtcctac acctagggga gaagcagcca aggggttgtt
tcccaccaag gacgacccgt ctgcgcacaa acggatgagc ccatcagaca aagacatatt
cattctctgc tgcaaacttg gcatagctct gctttgcctg gggctattgg gggaagttgc
ggttcgtgct cgcagggctc tcacccttga ctctttcaat aataactctt ctgtgcaaga
ttacaatcta aacaattcgg agaactcgac cttcctcctg aggcaaggac cacagccaac
ttcctcttac aagccgcatc gattttgtcc ttcagaaata gaaataagaa tgcttgctaa
aaattatatt tttaccaata agaccaatcc aataggtaga ttattagtta ctatgttaag
aaatgaatca ttatctttta gtactatttt tactcaaatt cagaagttag aaatgggaat
agaaaataga aagagacgct caacctcaat tgaagaacag gtgcaaggac tattgaccac
aggcctagaa gtaaaaaagg gaaaaaagag tgtttttgtc aaaataggag acaggtggtg
gcaaccaggg acttataggg gaccttacat ctacagacca acagatgccc ccttaccata
tacaggaaga tatgacttaa attgggatag gtgggttaca gtcaatggct ataaagtgtt
atatagatcc ctcccctttc gtgaaagact cgccagagct agacctcctt ggtgtatgtt
gtctcaagaa aagaaagacg acatgaaaca acaggtacat gattatattt atctaggaac
aggaatgcac ttttggggaa agattttcca taccaaggag gggacagtgg ctggactaat
agaacattat tctgcaaaaa cttatggcat gagttattat gattagcctt gatttgccca
accttgcggt tcccaaggct taagtaagtt tttggttaca aactgttctt aaaacaagga
tgtgagacaa gtggtttcct gacttggttt ggtatcaaag gttctgatct gagctctgag
tgttctattt tcctatgttc ttttggaatt tatccaaatc ttatgtaaat gcttatgtaa
accaagatat aaaagagtgc tgattttttg agtaaacttg caacagtcct aacattcacc
tcttgtgtgt ttgtgtctgt tcgccatccc gtctccgctc gtcacttatc cttcactttc
cagagggtcc ccccgcagac cccggcgacc ctcaggtcgg ccgactgcgg cagctggcgc
ccgaacaggg accctcggat aagtgaccct tgtctttatt tctactattt tgtgttcgtc
ttgttttgtc tctatcttgt ctggctatca tcacaagagc ggaacggact cacctcaggg
aaccaa
//