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ID   PMDSG      preliminary; circular DNA; SYN; 7626 BP.
XX
AC   IG0091;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMDSG - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSV2-dhfr from SV40 & pBR322 & mouse DHFR gene
RC   pSV0-dhfr from pSV2-dhfr
RC   pSVMdhfr from pSV2-dhfr & MMTV
RC   pMTVdhfr from pSVMdhfr
RC   pMSG or pMDSG from pMTVdhfr & E.coli gpt gene
RA   Lee F., Mulligan R.C., Berg P., Ringold G.M.;
RT   "Glucocorticoids regulate expression of dihydrofolate reductase
RT   cDNA in mouse mammary tumour virus chimaeric plasmids";
RL   Nature 294:228-232(1981).
XX
RN   [2]
RC   pSVMgpt from pSV2-gpt & MMTV LTR
RA   Chapman A.B., Costello M.A., Lee F., Ringold G.M.;
RT   "Amplification and hormone-regulated expression of a mouse mammary
RT   tumor virus-Eco gpt fusion plasmid in mouse 3T6 cells";
RL   Mol. Cell. Biol. 3:1421-1429(1983).
XX
RN   [3]
RC   pLC2 from MMTV LTR & pLC1
RC   pGTC1 from MMTV LTR & pLC1
RC   pMC1 from MMTV LTR & pLC1
RC   pMC2 from pLC1
RC   pGMC1.5A/B, pGMC1.3A/B from pMC1 & GRE
RC   pNMC1 from pMC1 & linker
RC   pNMC2, pNMC3 from pMC1 & linker
RA   Morley K.L., Toohey M.G., Peterson D.O.;
RT   "Transcriptional repression of a hormone-responsive promoter";
RL   Nucleic Acids Res. 15:6973-6989(1987).
XX
RN   [4]
RC   pSV01 from pBR322 & SV40 ori & linker
RC   pSV0d from pML-RIIG & pSV01
RC   pSV0alpha series from pSV0d & human alpha-globin gene
RA   Mellon P., Parker V., Gluzman Y., Maniatis T.;
RT   "Identification of DNA sequences required for transcription of the
RT   human alpha 1-globin gene in a new SV40 host-vector system";
RL   Cell 27:279-288(1981).
XX
RN   [5]
RC   plasmid from pSV0d & linker
RC   pMT1 from plasmid & pZ152
RC   plasmid2 from pMT1 & pSV2-cat
RC   pLC1 from plasmid2 & MMTV LTR
RC   pLC2 from pLC1 & pUC18
RC   pLCL1 from pLC1 & MMTV LTR
RC   p23LC1 from pMTV1 & pLC1
RA   Toohey M.G., Morley K.L., Peterson D.O.;
RT   "Multiple hormone-inducible enhancers as mediators of differential
RT   transcription";
RL   Mol. Cell. Biol. 6:4526-4538(1986).
XX
RN   [6]
RC   pMTV1 from MMTV
RA   Ucker D.S., Ross S.R., Yamamoto K.R.;
RT   "Mammary tumor virus DNA contains sequences required for its
RT   hormone regulated transcription";
RL   Cell 27:257-266(1981).
XX
CC   mouse mammary tumor virus long terminal repeat is a glucocorticoid
CC   -inducible promoter, e.g., by dexamethasone.
CC   uses first ATG in inserted DNA.
CC   NM (pMDSG)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli HB101)(hormonally responsive mammal)
CC   HO (Chinese hamster ovary CHO)(human HeLa)(mouse Ltk-)(mouse 3T6)
CC   CP ()
CC   FN (expression shuttle)(expression inducible)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF (pMSG-CAT)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. SV40, ori/early splice/polyA
FT                   3. mouse, DHFR gene
FT                   -> pSV2-dhfr [unique HindIII]
FT                   1. pSV2-dhfr
FT                   -> pSV0-dhfr
FT                   1. pSV2-dhfr HindIII
FT                   2. MMTV PstI-PstI 1450bp, MMTV LTR
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   HindIII linker 6bp aagctt:HindIII linker 6bp aagctt
FT                   HindIII-HindIII
FT                   -> pSVMdhfr
FT                   1. pSV2-dhfr remove PvuII-HindIII, SV40 early promoter
FT                   HindIII linker 6bp aagctt:
FT                   2. MMTV PstI-PstI 1450bp, MMTV LTR
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   HindIII linker 6bp aagctt:HindIII linker 6bp aagctt
FT                   HindIII-HindIII
FT                   -> pMTVdhfr
FT                   1. pMTVdhfr BamHI
FT                   2. pSV2-gpt PvuII-BamHI 2200bp, SV40 ori/early/gpt
FT                   BamHI linker 6bp ggatcc:
FT                   -> pMDSG 7626bp"
FT   misc_binding    1..23
FT                   /note="MCS unique NheI-SmaI-SalI-XhoI"
FT   misc_feature    >23..<877
FT                   /note="SPL SV40 early genes"
FT   polyA_signal    >23..<877
FT                   /note="PLA SV40 early genes"
FT   rep_origin      871..1217
FT                   /note="ORI SV40"
FT   promoter        871..1217
FT                   /note="PRO SV40 early genes"
FT   CDS             1217..<2988
FT                   /note="GEN E. coli xanthine-guanine
FT                   phosphoribosyl-transferase (gpt) gene"
FT   misc_binding    1237..1237
FT                   /note="SIT unique BglII"
FT   misc_binding    1535..1535
FT                   /note="SIT unique KpnI"
FT   misc_feature    0..0
FT                   /note="SPL SV40 early genes"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   misc_binding    3872..3872
FT                   /note="SIT unique EcoRI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    4498..4498
FT                   /note="SIT unique PvuI"
FT   rep_origin      >4700..<6011
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    6011..6011
FT                   /note="SIT unique Tth111I"
FT   misc_binding    6171..6171
FT                   /note="SIT HindIII"
FT   promoter        6171..7626
FT                   /note="PRO mouse mammary tumor virus (MMTV)
FT                   long terminal repeat (LTR), PstI-PstI fragment"
FT   misc_binding    6498..6498
FT                   /note="SIT unique ClaI"
FT   misc_binding    7251..7251
FT                   /note="SIT unique SacI"
XX
SQ   Sequence 7626 BP; 2081 A; 1735 C; 1694 G; 2116 T; 0 other;
     gctagcccgg ggtcgacctc gagggatctt tgtgaaggaa ccttacttct gtggtgtgac
     ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
     taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
     tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
     taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
     tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     ccaaactcat caatgtatct tatcatgtct ggatccgctg tggaatgtgt gtcagttagg
     gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
     cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     ggaaaggcat tattgccgta agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg
     aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
     gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
     acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
     ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
     tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
     tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
     ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
     ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
     cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
     cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
     atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
     tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt
     tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
     tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
     ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
     tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     ggatccccag gaagctcctc tgtgtcctca taaaccctaa cctcctctac ttgagaggac
     attccaatca taggctgccc atccaccctc tgtgtcctcc tgttaattag gtcacttaac
     aaaaaggaaa ttgggtaggg gtttttcaca gaccgctttc taagggtaat tttaaaatat
     ctgggaagtc ccttccactg ctgtgttcca gaagtgttgg taaacagccc acaaatgtca
     acagcagaaa catacaagct gtcagctttg cacaagggcc caacaccctg ctcatcaaga
     agcactgtgg ttgctgtgtt agtaatgtgc aaaacaggag gcacattttc cccacctgtg
     taggttccaa aatatctagt gttttcattt ttacttggat caggaaccca gcactccact
     ggataagcat tatccttatc caaaacagcc ttgtggtcag tgttcatctg ctgactgtca
     actgtagcat tttttggggt tacagtttga gcaggatatt tggtcctgta gtttgctaac
     acaccctgca gctccaaagg ttccccacca acagcaaaaa aatgaaaatt tgacccttga
     atgggttttc cagcaccatt ttcatgagtt ttttgtgtcc ctgaatgcaa gtttaacata
     gcagttaccc caataacctc agttttaaca gtaacagctt cccacatcaa aatatttcca
     caggttaagt cctcatttaa attaggcaaa ggaattcttg aagacgaaag ggcctcgtga
     tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc aagcttggca
     gaaatggttg aactcccgag agtgtcctac acctagggga gaagcagcca aggggttgtt
     tcccaccaag gacgacccgt ctgcgcacaa acggatgagc ccatcagaca aagacatatt
     cattctctgc tgcaaacttg gcatagctct gctttgcctg gggctattgg gggaagttgc
     ggttcgtgct cgcagggctc tcacccttga ctctttcaat aataactctt ctgtgcaaga
     ttacaatcta aacaattcgg agaactcgac cttcctcctg aggcaaggac cacagccaac
     ttcctcttac aagccgcatc gattttgtcc ttcagaaata gaaataagaa tgcttgctaa
     aaattatatt tttaccaata agaccaatcc aataggtaga ttattagtta ctatgttaag
     aaatgaatca ttatctttta gtactatttt tactcaaatt cagaagttag aaatgggaat
     agaaaataga aagagacgct caacctcaat tgaagaacag gtgcaaggac tattgaccac
     aggcctagaa gtaaaaaagg gaaaaaagag tgtttttgtc aaaataggag acaggtggtg
     gcaaccaggg acttataggg gaccttacat ctacagacca acagatgccc ccttaccata
     tacaggaaga tatgacttaa attgggatag gtgggttaca gtcaatggct ataaagtgtt
     atatagatcc ctcccctttc gtgaaagact cgccagagct agacctcctt ggtgtatgtt
     gtctcaagaa aagaaagacg acatgaaaca acaggtacat gattatattt atctaggaac
     aggaatgcac ttttggggaa agattttcca taccaaggag gggacagtgg ctggactaat
     agaacattat tctgcaaaaa cttatggcat gagttattat gattagcctt gatttgccca
     accttgcggt tcccaaggct taagtaagtt tttggttaca aactgttctt aaaacaagga
     tgtgagacaa gtggtttcct gacttggttt ggtatcaaag gttctgatct gagctctgag
     tgttctattt tcctatgttc ttttggaatt tatccaaatc ttatgtaaat gcttatgtaa
     accaagatat aaaagagtgc tgattttttg agtaaacttg caacagtcct aacattcacc
     tcttgtgtgt ttgtgtctgt tcgccatccc gtctccgctc gtcacttatc cttcactttc
     cagagggtcc ccccgcagac cccggcgacc ctcaggtcgg ccgactgcgg cagctggcgc
     ccgaacaggg accctcggat aagtgaccct tgtctttatt tctactattt tgtgttcgtc
     ttgttttgtc tctatcttgt ctggctatca tcacaagagc ggaacggact cacctcaggg
     aaccaa
//