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ID   PMEX6      preliminary; circular DNA; SYN; 3616 BP.
XX
AC   IG1091;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pMEX6 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pAX1 from pSS20*c & oligo
RC   pAX2 from pAX1 & pSS9
RC   pAX3+, pAX3- from pAX2 & pEMBL8+
RC   pAX4+ from pAX3+
RC   pAX4- from pAX3+
RC   pAX4a+ from pAX4+ & linker
RC   pAX4b+ from pAX4+ & linker
RC   pAX4c+ from pAX4+ & linker
RC   pAX4a- from pAX4- & linker
RC   pAX4b- from pAX4- & linker
RC   pAX4c- from pAX4- & linker
RC   pAR10 from pAX1 & E.coli HisRS
RC   pPM3220 from pAX4+ & ILS1 gene
RA   Markmeyer P., Ruhlmann A., Englisch U., Cramer F.;
RT   "The pAX plasmids: new gene-fusion vectors for sequencing,
RT   mutagenesis and expression of proteins in Escherichia coli";
RL   Gene 93:129-134(1990).
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CC   stop codons in all 3 reading frames.
CC   Use site-directed mutagenesis to shorten distance between
CC   promoter and RBS to 5-20 bases.
CC   NM (pMEX6)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (coding)
CC   TY (phagemid)
CC   SP (USB)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(expression)(sequencing)(mutagenesis)
CC   SE ()
CC   PA (pBR322)(f1)
CC   BR (pMEX5)(pMEX7)(pMEX8)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 remove HindIII-PstI 16bp 400..416,
FT                   \ MCS/2670bp
FT                   2. E. coli PstI-HindIII 800bp, rrnB operon T1T2
FT                   -> pMEX6 3616bp"
FT   promoter        >3433..3613
FT                   /note="PRO E. coli tac (trp and lac)"
FT   misc_binding    3614..50
FT                   /note="MCS unique EcoRI-AvaI-SmaI-SalI-BsaBI-EcoRV-
FT                   ClaI-BanIII-PstI-SphI-HindIII"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB operon T1"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB operon T2"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    832..832
FT                   /note="SIT unique BcgI"
FT   misc_binding    876..876
FT                   /note="SIT unique ScaI"
FT   misc_binding    987..987
FT                   /note="SIT unique PvuI"
FT   misc_binding    1134..1134
FT                   /note="SIT unique FspI"
FT   misc_binding    1235..1235
FT                   /note="SIT unique BglI"
FT   misc_binding    1833..1833
FT                   /note="SIT unique AlwNI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    2247..2247
FT                   /note="SIT unique AflIII"
FT   misc_binding    2273..2273
FT                   /note="SIT unique TfiI"
FT   misc_binding    2425..2425
FT                   /note="SIT unique NdeI"
FT   misc_binding    2500..2500
FT                   /note="SIT unique Tth111I"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    2887..2887
FT                   /note="SIT unique DraIII"
FT   misc_binding    3206..3206
FT                   /note="SIT unique EcoO109I"
FT   misc_binding    3236..3236
FT                   /note="SIT unique Eco47III"
FT   misc_binding    3358..3358
FT                   /note="SIT unique MroI"
FT   misc_binding    3433..3433
FT                   /note="SIT unique BspMI"
XX
SQ   Sequence 3616 BP; 869 A; 914 C; 926 G; 907 T; 0 other;
     ttcccgggga tccgtcgacg atatcgatct gcagcatgct gaatgaatga agcttctgtt
     ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc
     tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
     actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
     ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     ccgaaacgcg cgaggcagac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttaaatat
     ttgcttatac aatcttcctg tttttggggc ttttctgatt atcaaccggg gtcaaggaga
     tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
     tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
     cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcc
     ggagcttatc gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg
     tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactcgc
     gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga
     gctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
     cacacaggaa acagaa
//