back Return to this vector's summary.
ID   PMEX7      preliminary; circular DNA; SYN; 3631 BP.
XX
AC   IG1092;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pMEX7 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pAX1 from pSS20*c & oligo
RC   pAX2 from pAX1 & pSS9
RC   pAX3+, pAX3- from pAX2 & pEMBL8+
RC   pAX4+ from pAX3+
RC   pAX4- from pAX3+
RC   pAX4a+ from pAX4+ & linker
RC   pAX4b+ from pAX4+ & linker
RC   pAX4c+ from pAX4+ & linker
RC   pAX4a- from pAX4- & linker
RC   pAX4b- from pAX4- & linker
RC   pAX4c- from pAX4- & linker
RC   pAR10 from pAX1 & E.coli HisRS
RC   pPM3220 from pAX4+ & ILS1 gene
RA   Markmeyer P., Ruhlmann A., Englisch U., Cramer F.;
RT   "The pAX plasmids: new gene-fusion vectors for sequencing,
RT   mutagenesis and expression of proteins in Escherichia coli";
RL   Gene 93:129-134(1990).
XX
RN   [2]
RC   pMEX7, pMEX8 from phage f1 & ptac series & pBR322
RA   Buttcher V., Ruhlmann A., Cramer F.;
RT   "Improved single-stranded DNA producing expression vectors for
RT   protein manipulation in Escherichia coli";
RL   Nucleic Acids Res. 18:1075-1075(1990).
XX
CC   stop codons in all 3 reading frames.
CC   Use site-directed mutagenesis to shorten distance between
CC   promoter and RBS to 5-20 bases.
CC   NM (pMEX7)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (non-coding)
CC   TY (phagemid)
CC   SP (USB)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(expression)(sequencing)(mutagenesis)
CC   SE ()
CC   PA (pBR322)(f1)
CC   BR (pMEX6)(pMEX5)(pMEX8)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 remove HindIII-PstI 16bp 400..416,
FT                   \ MCS/2670bp
FT                   2. E. coli PstI-HindIII 800bp, rrnB operon T1T2
FT                   -> plasmid 3500bp
FT                   1. plasmid 3500bp
FT                   2. E. coli tac promoter
FT                   -> pMEX7 3631bp"
FT   promoter        >3448..3628
FT                   /note="PRO E. coli tac (trp and lac)"
FT   misc_binding    3629..65
FT                   /note="MCS unique EcoRI-StyI-Asp718-KpnI-ApaI-AvaI-
FT                   SmaI-BstEII-SalI-EcoRV-ClaI-BanIII-EcoT22I-AvaIII-
FT                   SphI-PstI-HindIII"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB operon T1"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB operon T2"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    891..891
FT                   /note="SIT unique ScaI"
FT   misc_binding    1002..1002
FT                   /note="SIT unique PvuI"
FT   misc_binding    1149..1149
FT                   /note="SIT unique FspI"
FT   misc_binding    1250..1250
FT                   /note="SIT unique BglI"
FT   misc_binding    1848..1848
FT                   /note="SIT unique AlwNI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    2262..2262
FT                   /note="SIT unique AflIII"
FT   misc_binding    2288..2288
FT                   /note="SIT unique TfiI"
FT   misc_binding    2440..2440
FT                   /note="SIT unique NdeI"
FT   misc_binding    2515..2515
FT                   /note="SIT unique Tth111I"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    2951..2951
FT                   /note="SIT unique DraIII"
FT   misc_binding    3251..3251
FT                   /note="SIT unique Eco47III"
FT   misc_binding    3370..3370
FT                   /note="SIT unique BamHI"
FT   misc_binding    3373..3373
FT                   /note="SIT unique MroI"
FT   misc_binding    3448..3448
FT                   /note="SIT unique BspMI"
XX
SQ   Sequence 3631 BP; 927 A; 898 C; 951 G; 855 T; 0 other;
     ttccatgggt accagggccc gggttaccgt cgacgatatc gatgcatgct gcagtgaatg
     aatgaagctt ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag
     aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
     ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc
     cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
     tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg
     ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
     tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     ttttcaccgt catcaccgaa acgcgcgagg cagaccccgg ttgataatca gaaaagcccc
     aaaaacagga agattgtata agcaaatatt taaattgtaa acgttaatat tttgttaaaa
     ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     ggcgcgtcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     cggcgtagag gatccggagc ttatcgactg cacggtgcac caatgcttct ggcgtcaggc
     agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc
     tcaaggcgca ctcgcgttct ggataatgtt ttttgcgccg acatcataac ggttctggca
     aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg
     agcggataac aatttcacac aggaaacaga a
//