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ID   PMG280     preliminary; circular DNA; SYN; 4659 BP.
XX
AC   A15353; A15354;
XX
DT   01-FEB-1995 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid pMG820 - lactose region.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-4659
RC   pMG820 from pBR322
RA   De Vos W.M.;
RT   "Process for selecting and maintaining recombinant DNA in lactic
RT   acid bacteria";
RL   Unpublished (1986).
RL   European Patent No. 0355036-A 1 dated 21-FEB-1990.
RL   NEDERLANDS INSTITUUT VOOR ZUIVELONDERZOEK.
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CC   NCBI gi: 491900
CC   NM (pMG820)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   -> pMG820 4659bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 4659 BP; 1385 A; 855 C; 957 G; 1462 T; 0 other;
     tggctccaga tgacaaacat gatctcatat cagggacttg ttcgcacctt ccttaacaaa
     gttcttgtta aaacagctag tccatggact gataaagtat agtaaaaaca taaaacggag
     gatattgttg tgaacagaga agagatgact ctcttagggt ttgaaattgt tgcttatgct
     ggagatgctc gctctaagct tttagaagcg cttaaagcgg ctgaaaatgg tgatttcgct
     aaggcagata gtcttgtagt agaagcagga agctgtattg cagaggctca cagttctcag
     acaggtatgt tggctcgaga accttctggg gaggaacttc catacagtgt tactatgatg
     catggtcagg atcacttgat gactacgatc ttattaaaag atgtgattca tcacctcatc
     gaactttata aaagaggagc aaagtaatta atgcataaac tcattgaact tattgagaaa
     gggaaaccat tctttgagaa aatctctcga aacatctatc ttcgtgctat tcgtgatgga
     tttattgctg gtatgccagt catccttttc tcatctatct ttatccttat tgcctatgtc
     ccaaatgctt ggggattcca ctggtcaaaa gacattgaga ccttcttgat gactccttat
     agctattcga tgggtatcct agcattcttt gttggtggta ctaccgctaa agctttgacg
     gactcaaaga accgtgacct acctgcgacc aatcagatta acttcttatc tacgatgcta
     gcgtctatgg tagggttcct tttgatggca gctgagcctg caaaagaagg aggcttcttg
     acagccttca tgggaacaaa aggccttttg acagcattta tcgcagcctt tgtgacagtt
     aatgtttata aagtctgtgt aaaaaataat gttaccattc gtatgcctga agatgttcca
     ccaaatatct ctcaagtatt taaggacttg attccgttca ccgtatcagt tgttctccta
     tatggacttg aactccttgt taagggaact cttggtgtaa ctgttgcaga atcaatcggt
     acccttattg ctcctctttt ctcagctgca gatggttacc ttggaattac acttatcttt
     ggtgcttatg ccttcttctg gtttgttggt attcacggtc cttcaattgt cgaaccagca
     atcgctgcta tcacttatgc caatatcgat gtcaacttgc atcttatcca agctggacaa
     catgcagata aagttatcac ttctggtact caaatgttta ttgctaccat gggtggaaca
     ggagctacat tgattgttgc attcttgttc atgtggattt gtaaatcaga tcgtaaccgt
     gccatcggac gtgcctcagt tgttccaaca ttctttgggg ttaatgagcc aatcttattt
     ggtgcaccaa tcgtattgaa tccgattttc tttgtaccgt tcattttcgc tccgattgtc
     aacgtttgga tctttaaatt ctttgttgac acattgaaca tgaactcatt ctctgccaac
     cttccatagg ttactcctgg tccgttggga attgtactcg gtactaactt ccaagttcta
     tcatttatct tagccggact cttggtagtt gttgatacta tcatttatta cccatttgtt
     aaggtatacg atgaacaaat tcttgaagaa gaacgttctg gtaaaacaaa cgatgctctt
     aaagaaaaag tagctgcaaa cttcaatact gctaaagcag atgccgttct tggaaaagca
     gacattggta aagaagatat agcagcaaac aataatatca caaaagaaac taatgtcctt
     gttctttgtg caggtggagg tacaagtggc ttgcttgcca gtgccttgaa caaagcagca
     gcagaataca atattccagt gaaggcggca gcgggtggtt acggtgctca ccgtgaaatg
     ttgccagagt ttgacttggt aatcttggct ccacaggtcg cttcaaactt cgatgatatg
     aaggctgaaa cagataaatt gggcattaaa cttgtaaaaa ctgaaggagc tcaatatatc
     aaattgacac gtgacgcaca aggtgctctt gcatttgttc aacaacagtt tgattaataa
     atttgtaaaa gttatttgag acgagatcac tcattccatt tccttggttt tcagaacaca
     gaatctcgtc tcaaatgctt tttttgaaag gacttacact tatgactaaa acacttccta
     aagattttat ttttggtggt gctacagctg cttatcaagc agaaggtgca actcacacag
     atggtaaggg cccagtagca tgggataagt atcttgaaga caattattgg tatacagcag
     aacctgcaag taatttttac cataaatacc cagttgattt agaattagct gaagaatatg
     gtgttaatgg tattcgtatt tctattgcct ggtctcgtat tttcccaacg ggttacggtg
     aagtaaatga aaaaggtgtt gaattctacc ataaactatt tgctgaatgc cacaagcgtc
     atgttgaacc atttgtgact ttgcatcatt ttgacacacc agaagctctt cattcaaatg
     gagatttctt aaaccgcgaa aatatagaac actttataga ttatgccgct ttctgttttg
     aagagtttcc agaagtaaac tactggacaa ctttcaatga aattggccca attggtgatg
     gtcaatactt ggttggtaaa ttccctccag gcattaaata tgatcttgcc aaagtcttcc
     aatcacacca caacatgatg gtctctcatg cacgtgctgt aaaattgtat aaggataaag
     gttataaagg tgaaattggt gttgttcatg ctttaccaac taaataccct tatgatccgg
     aaaatccagc agatgttcgt gctgctgaac ttgaagacat catccataac aaatttatct
     tggatgcaac ctatcttggg cactattcag ataaaacaat ggaaggtgtc aaccatatcc
     tagctgagaa tggtggagaa cttgatcttc gtgatgaaga cttccaagct cttgacgcag
     ctaaagattt gaatgatttc cttggtatca actattacat gagtgattgg atgcaagctt
     ttgatggtga gactgaaatc attcacaatg gtaaggctga aaaaggaagc tctaagtacc
     aaattaaggg tgttggtcgt cgagtagctc ctgactatgt tccgcgcaca gactgggatt
     ggattattta tcctgaaggc ttgtatgacc aaatcatggg agtgaaaaat gattatccga
     attacaagaa gatttacatc actgaaaacg gtctcggata caaagatgag tttgtagata
     atactgttta tgatgatggt cgtattgatt acgtgaagca acacttggaa gttttatcag
     acgcgattgc ggatggtgca aatgttaaag gttacttcat ttggtcactt atggacgttt
     tctcatggtc aaatggttat gaaaaacgtt atggattgtt ctatgtagac tttgatacgc
     aagaacgcta tcctaagaaa tcagcacatt ggtataagaa attagcagaa actcaagtga
     tagagtaata tcacctgaga atgtaataat ttgttatctg atttttagat caggctttga
     aaaggtatat attattaatt tcgaagattg tcctataatt cctgagcttg aagctacctc
     actacaaaat gtagtaactg gtatgtttca cctggaaaag aaaggatttg ccattctgat
     ttagcattat ccgaatttga atgggaagtt ttacaattac tcgatgtagc aatatgtgag
     actgttcact tcgaggtaga ttatttgcct ttccaataac aaaaagaaag gaattgactg
     tgactatcga attaaaaaac gagtatctta cggttcagtt taaaacactt ggtggtcaac
     tgacttcgat taaagacaaa gatgggttag aatatctctg gcaagctgat ccagagtatt
     ggaatggaca ggcaccaatt ctatttccta tctgtggtag tttacgtaat gattgggcta
     tttatagacc tcaagaaaga ccttttttta cagctctaat tcgtagacat ggttttgttc
     gaaaagaaga atttactctt gaagaagtta atgaaaatag cgtgactttc agtattaaac
     caaatgctga gatgcttgat aattaccttt atcagtttga actaagagtt gtttatacct
     taaatgggaa atccattaga accgagtttc aggtaacgaa cttggaaact gaaaaaacga
     tgccttactt cattggagcc catccagcat tcaattgtcc tttagtagaa ggcgaaaagt
     atgaggacta ttctcttgaa tttagtgagg ttgagtcttg ttcgatacca aagagtttcc
     ctgagacagg gttattagat ttacaagatc ggacgccatt tttggaaaac caaaagtctc
     ttgatttgga ttactcactt ttttctcatg acgccattac acttgaccga ctgaaatctc
     gcagcgtgac acttcgctcg accgatgccc ttgagagcc
//