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ID   PMK20      preliminary; circular DNA; SYN; 4028 BP.
XX
AC   X13003;
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DT   20-OCT-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pMK20 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-4028
RC   pMK20
RA   Vezina G.;
RT   ;
RL   Submitted (24-SEP-1988) on tape to the EMBL Data Library by:
RL   Vezina G., Laval University, Dept. of Microbiology and Immunology,
RL   Faculty of Medicine, Quebec PQ G1K 7P4, Canada.
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RN   [2]
RC   pMK20 from ColE1
RA   Vezina G., Levesque R.C.;
RT   "Nucleotide sequence of pMK20";
RL   Nucleic Acids Res. 16:0-0(1988).
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RN   [3]
RC   from pMK16 or pTK16
RC   from pMK20
RC   from pMK2004
RC   from pDF41
RC   from pRK353
RC   pRK2501 from pRK248 & kan gene
RA   Kahn M., Kolter R., Thomas C., Figurski D., Meyer R., Remaut E.,
RA   Helinski D.R.;
RT   "Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and
RT   RK2";
RL   Meth. Enzymol. 68:268-280(1979).
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RN   [4]
RC   pMK16 or pTK16 from ColE1
RC   pMK16.1 from pMK16
RA   Helinski D.R.;
RT   ;
RL   Genetic Engineering for Nitrogen Fixation 0:19-19(1977).
RL   Hollaender A., ed., Plenum, New York.
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RN   [5]
RC   pDF1 from mini-F & ColE1
RC   pDF11 from F' lac
RC   pIF1 from F' lac
RC   pIF11 from F' lac
RC   pDF31 from F' lac
RC   pDF38 from F' lac
RC   pDF41 from F' lac
RC   pDF42 from ColE1 & F' lac
RC   pDF71 from F' lac
RA   Figurski D.;
RT   ;
RL   Unpublished (1977).
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RN   [6]
RC   pMK31 from amp gene
RC   pMK2005 from ColE1 & trpE gene
RA   Kahn M.;
RT   ;
RL   Unpublished (1977).
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RN   [7]
RC   pRK646 from R6K
RA   Kolter R.;
RT   ;
RL   Unpublished (1977).
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CC   NM (pMK20)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (ColE1)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. ColE1 remove 2618bp, 4028bp
FT                   -> pMK20 4028bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    complement(2799..4028,1..1009)
FT                   /note="HaeII A fragment, from ColE1"
FT   misc_feature    1010..1368
FT                   /note="HaeII E fragment, from ColE1"
FT   CDS             complement(1654..2472)
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo),
FT                   kanamycin resistance gene (kan) (AA 1-271)"
FT   misc_feature    complement(1369..2798)
FT                   /note="HaeII fragment, from Tn903,
FT                   E. coli kanamycin resistance gene"
XX
SQ   Sequence 4028 BP; 1181 A; 845 C; 926 G; 1076 T; 0 other;
     ttctatgctc ctatattgat aagaataaac ttaatactat aaatgaggtg ttagggattt
     aattattctt tattgatata aaaagtccta gcaatccaaa tgggattgct aggaccaaac
     aaagtagatt atatagcata aataggttta attttgctac gggggcgtta tttaggtttt
     ttcttctttc gaaaaaatct ttctttatga agttaaaagc tatgtattca atagcatatt
     ttgaatatgg acatagaata gtgcttatca ctattgcata tagcatctta tctgacacaa
     ggaaataata cccttcgctg ttttttgtta taaggtatat atatataagt gtgcagtaca
     ggccaaataa aatatttttt atgtagtatc ttaagctcat aaattaaacc tcgccatata
     ttcttttcat tttataagga tcgagttatg aggaaaagat tttttgtggg aatattcgcg
     ataaacctcc ttgttggatg tcaggctaac tatatacctg atgttcaggg agggaccatc
     gcaccatcct cctcttctaa actgacgggg atcgcggttc agtagaaaag attaaaggat
     cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     taccaacggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag tcgggccact
     acttcaagaa ctctgtagca ccgtttgtgc catcatcgct ctgctaatcc ggttaccagt
     ggctgctgcc agtggcgtta aggcgtgcct taccgggttg gactcaagac gatagttacc
     ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     aacgacctac accgaactga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc
     cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     gagggagctt ccagggggaa acgcctggta gctttatagt cctgtcgggt ttcgccacct
     ctgacttgag cgtctatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     ctgctacgtg gccttcttcc tgttcctggt cttttgctca catgttcttt ccggccttat
     cccctgattc tgtggataac tgtgttaccg tttttgtgtg agtcagtacc gctcgccgca
     gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc tgaggtctgc
     ctcgtgaaga aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga
     aagtgaggga gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga
     acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca
     actcagcaaa agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct
     ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg
     aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt
     atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat
     atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
     tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg
     ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc
     agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct acaacaaagc
     tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc aggcctggta
     tgagtcagca acaccttctt cacgaggcag acctcagcgc ctgaaggata tcgtgaatga
     ggctcttcgt cacaatgcct cacgtacgcc ttcagcaaca gagcttgctc atgctaataa
     tgcagctatg caggcggaag cagagcgttt gcgccttgcg aaagcagaag aaaaagcccg
     taaagaagcg gaagcagcag aaaaggcttt tcaggaagca gaacaacgac gtaaagagat
     tgaacgggag aaggctgaaa cagaacgcca gttgaaactg gctgaagctg aagagaaacg
     actggctgca ttgagtgaag aagctaaagc tgttgagatc gcccaaaaaa aactttctgc
     tgcacaatct gaagtggtga aaatggatgg agagattaag actctcaatt ctcgtttaag
     ctccagtatc catgcccgtg atgcagaaat gaaaacgctc gctggaaaac gaaatgaact
     ggctcaggca tccgctaaat ataaagaact ggatgagctg gtcaaaaaac tatcaccaag
     agccaatgat ccgcttcaga accgtccttt ttttgaagca accagacgac gggttggggc
     cggtaagatt agagaagaaa aacaaaaaca ggtaacagca tcagaaacac gtattaaccg
     gataaatgct gatataactc agatccagaa ggctatttct caggtcagta ataatcgtaa
     tgccggtatc gctcgtgttc atgaagctga agaaaatttg aaaaaagcac agaataatct
     ccttaattca cagattaagg atgctgttga tgcaacagtt agcttttatc aaacgctgac
     tgaaaaatat ggtgaaaaat attcgaaaat ggcacaggaa cttgctgata agtctaaagg
     taagaaaatc ggcaatgtga atgaagctct cgctgctttt gaaaaataca aggatgtttt
     aaataagaaa ttcagcaaag ccgatcgtga tgctattttt aatgcgttgg catcggtgaa
     gtatgatgac tgggctaaac atttagatca gtttgccaag tacttgaaga ttacggggca
     tgtttctttt ggatatgatg tggtatctga tatcctaaaa attaaggata caggtgactg
     gaagccacta tttcttacat tagagaagaa agctgcagat gcaggggtga gttatgttgt
     tgctttactt tttagcttgc ttgctggaac tacattaggt atttggggta ttgctattgt
     tacaggaa
//