back Return to this vector's summary.
ID   PMK3       preliminary; circular DNA; SYN; 7213 BP.
XX
AC   ATCC37314;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Bacillus/E.coli plasmid vector pMK3 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pMK3 from pUB110 & pUC9
RC   pMK4 from pC194 & pUC9
RA   Sullivan M.A., Yasbin R.E., Young F.E.;
RT   "New shuttle vectors for Bacillus subtilis and Escherichia coli
RT   which allow rapid detection of inserted fragments";
RL   Gene 29:21-26(1984).
XX
RN   [2]
RC   pHV428 from pC194 & pUB110 & pBR322
RA   Ehrlich S.D., Niaudet B., Michel B.;
RT   "Use of plasmids from Staphylococcus aureus for cloning of DNA in
RT   Bacillus subtilis";
RL   Curr. Top. Microbiol. Immunol. 96:19-29(1981).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   A Bacillus subtilis/E.coli shuttle vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pMK3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli JM83)(Bacillus subtilis)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUB110)(pUC9)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUB110 BamHI 4548bp 789..789
FT                   2. pUC9 MboI/Sau3A 2665bp 254..254, MCS
FT                   -> pMK3 7213bp"
FT   -               1..253
FT                   /note="pUC9 1..253 253bp
FT                   BamHI =        G^GATCC
FT                   MboI = Sau3AI = ^GATC"
FT   -               254..4801
FT                   /note="pUB110 789..4548..788 4548bp
FT                   MboI = Sau3AI = ^GATC
FT                   BamHI =        G^GATCC"
FT   -               4802..7213
FT                   /note="pUC9 254..2665 2412bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-HindIII-SmaI-SalI-PstI-BglI-
FT                   TaqI-XbaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
XX
SQ   Sequence 7213 BP; 1961 A; 1679 C; 1295 G; 2278 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
     ctgcaggtcg acggatccag ttgctcaaaa aaatctcggt cagatgttac tagcaactca
     tttacaagaa cagcatcttt cctccgtttt tcttgtacct gttttttgtg attcaataat
     ttctttgaca cgttcgttgt aatcaatatt tttatcattt ttcaaatcat aattttcacg
     tgttcgctca tggtcaatat catcattcgt tctacttttt cgctctcttt gattatgaaa
     ttgcatgcct tttagtccag ctgatttcac tttttgcatt ctacaaactg cataactcat
     atgtaaatcg ctccttttta ggtggcacaa atgtgaggca ttttcgctct ttccggcaac
     cacttccaag taaagtataa cacactatac tttatattca taaagtgtgt gctctgcgag
     gctgtcggca gtgccgacca aaaccataaa acctttaaga cctttctttt ttttacgaga
     aaaaagaaac aaaaaaacct gccctctgcc acctcagcaa aggggggttt tgctctcgtg
     ctcgtttaaa aatcagcaag ggacaggtag tattttttga gaagatcact caaaaaatct
     ccacctttaa acccttgcca atttttattt tgtccgtttt gtctagctta ccgaaagcca
     gactcagcaa gaataaaatt tttattgtct ttcggttttc tagtgtaacg gacaaaacca
     ctcaaaataa aaaagataca agagaggtct ctcgtatctt ttattcagca atcgcgcccg
     attgctgaac agattaataa tagattttag ctttttattt gttgaaaaaa gctaatcaaa
     ttgttgtcgg gatcaattac tgcaaagtct cgttcatccc accactgatc ttttaatgat
     gtattggggt gcaaaatgcc caaaggctta atatgttgat ataattcatc aattccctct
     acttcaatgc ggcaactagc agtaccagca ataaacgact ccgcacctgt acaaaccggt
     gaatcattac tacgagagcg ccagcttcat cacttgcctc ccatagatga atccgaacct
     cattacacat tagaactgcg aatccatctt catggtgaac caaagtgaaa cctagtttat
     cgcaataaaa acctatactc tttttaatat ccccgactgg caatgccggg atagactgta
     acattctcac gcataaaatc ccctttcatt ttctaatgta aatctattac cttattatta
     attcaattcg ctcataatta atcctttttc ttattacgca aaatggcccg atttaagcac
     accctttatt ccgttaatgc gccatgacag ccatgataat tactaatact aggagaagtt
     aataaatacg taaccaacat gattaacaat tattagaggt catcgttcaa aatggtatgc
     gttttgacac atccactata tatccgtgtc gttctgtcca ctcctgaatc ccattccaga
     aattctctag cgattccaga agtttctcag agtcggaaag ttgaccagac attacgaact
     ggcacagatg gtcataacct gaaggaagat ctgattgctt aactgcttca gttaagaccg
     aagcgctcgt cgtataacag atgcgatgat gcagaccaat caacatggca cctgccattg
     ctacctgtac agtcaaggat ggtagaaatg ttgtcggtcc ttgcacacga atattacgcc
     atttgcctgc atattcaaac agctcttcta cgataagggc acaaatcgca tcgtggaacg
     tttgggcttc taccgattta gcagtttgat acactttctc taagtatcca cctgaatcat
     aaatcggcaa aatagagaaa aattgaccat gtgtaagcgg ccaatctgat tccacctgag
     atgcataatc tagtagaatc tcttcgctat caaaattcac ttccaccttc cactcaccgg
     ttgtccattc atggctgaac tctgcttcct ctgttgacat gacacacatc atctcaatat
     ccgaataggg cccatcagtc tgacgaccaa gagagccata aacaccaata gccttaacat
     catccccata tttatccaat attcgttcct taatttcatg aacaatcttc attctttctt
     ctctagtcat tattattggt ccattcacta ttctcattcc cttttcagat aattttagat
     ttgcttttct aaataagaat atttggagag caccgttctt attcagctat taataactcg
     tcttcctaag catccttcaa tccttttaat aacaattata gcatctaatc ttcaacaaac
     tggcccgttt gttgaactac tctttaataa aataattttt ccgttcccaa ttccacattg
     caataataga aaatccatct tcatcggctt tttcgtcatc atctgtatga atcaaatcgc
     cttcttctgt gtcatcaagg tttaattttt tatgtatttc ttttaacaaa ccaccatagg
     agattaacct tttacggtgt aaaccttcct ccaaatcaga caaacgtttc aaattctttt
     cttcatcatc ggtcataaaa tccgtatcct ttacaggata ttttgcagtt tcgtcaattg
     ccgattgtat atccgattta tatttatttt tcggtcgaat catttgaact tttacatttg
     gatcatagtc taatttcatt gcctttttcc aaaattgaat ccattgtttt tgattcacgt
     agttttctgt attcttaaaa taagttggtt ccacacatac caatacatgc atgtgctgat
     tataagaatt atctttatta tttattgtca cttccgttgc acgcataaaa ccaacaagat
     ttttattaat ttttttatat tgcatcattc ggcgaaatcc ttgagccata tctgacaaac
     tcttatttaa ttcttcgcca tcataaacat ttttaactgt taatgtgaga aacaaccaac
     gaactgttgg cttttgttta ataacttcag caacaacctt ttgtgactga atgccatgtt
     tcattgctct cctccagttg cacattggac aaagcctgga tttacaaaac cacactcgat
     acaactttct ttcgcctgtt tcacgatttt gtttatactc taatatttca gcacaatctt
     ttactctttc agccttttta aattcaagaa tatgcagaag ttcaaagtaa tcaacattag
     cgattttctt ttctctccat ggtctcactt ttccactttt tgtcttgtcc actaaaaccc
     ttgatttttc atctgaataa atgctactat taggacacat aatattaaaa gaaaccccca
     tctatttagt tatttgttta gtcacttata actttaacag atggggtttt tctgtgcaac
     caattttaag ggttttcaat actttaaaac acatacatac caacacttca acgcaccttt
     cagcaactaa aataaaaatg acgttatttc tatatgtatc aagataagaa agaacaagtt
     caaaaccatc aaaaaaagac accttttcag gtgctttttt tattttataa actcattccc
     tgatctcgac ttcgttcttt ttttacctct cggttatgag ttagttcaaa ttcgttcttt
     ttaggttcta aatcgtgttt ttcttggaat tgtgctgttt tatcctttac cttgtctaca
     aaccccttaa aaacgttttt aaaggctttt aagccgtctg tacgttcctt aaggaattcc
     ttagtgcttt catagattaa actcacatca cgctttaaat cgcttatttt agactttaaa
     gacttgtttt cttcaagcaa ctcattataa tcatttacat tttcattaaa tcgctctaca
     agaccactat atttttcttt aacttgccca tgttctttac ttaatttttt atattctctc
     gccatatcag tactcatgag atttctaaca tgctgtttta acctatcgtt atctctcgca
     gcagtcacta agtttttata atcacgctcc gatataacaa catttttggt tggtttcttt
     tctgttttca ttatttcttt tcccaaacca aacatggact tttcacccgt tggcacttca
     acacttttca tgtgtcgttt cgctggtact tctaaatctg atttaacttt atcgctataa
     gcagtccatt catctttttt aactgctaaa tttttttcta gaaaatcaat ctctttttcc
     aaagtttgtt ttttaaattt agctgtctca atatgtttac ggtcagagcc acgttcacca
     cgcttcaact caaaaccctg ttttttcata tgctcgggga atttatcttg tagccataac
     agttcttgac gattaaacac attttttcct tgcagttttc catcacgcat aggcacaaca
     cctaaatgca tgtgaggggt ttgctcatca ttatgaactg ttgcataagc aatattttgc
     ttgccatatc gttcggaaaa taatttataa ctttcctcaa aaaatcgttt ttgttctcct
     ggatccccgg gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     aggaagcgga aga
//