Return to this vector's summary.
ID PMK3 preliminary; circular DNA; SYN; 7213 BP.
XX
AC ATCC37314;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Bacillus/E.coli plasmid vector pMK3 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pMK3 from pUB110 & pUC9
RC pMK4 from pC194 & pUC9
RA Sullivan M.A., Yasbin R.E., Young F.E.;
RT "New shuttle vectors for Bacillus subtilis and Escherichia coli
RT which allow rapid detection of inserted fragments";
RL Gene 29:21-26(1984).
XX
RN [2]
RC pHV428 from pC194 & pUB110 & pBR322
RA Ehrlich S.D., Niaudet B., Michel B.;
RT "Use of plasmids from Staphylococcus aureus for cloning of DNA in
RT Bacillus subtilis";
RL Curr. Top. Microbiol. Immunol. 96:19-29(1981).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC A Bacillus subtilis/E.coli shuttle vector.
CC Medium is 1227 LB plus ampicillin.
CC NM (pMK3)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli JM83)(Bacillus subtilis)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pUB110)(pUC9)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUB110 BamHI 4548bp 789..789
FT 2. pUC9 MboI/Sau3A 2665bp 254..254, MCS
FT -> pMK3 7213bp"
FT - 1..253
FT /note="pUC9 1..253 253bp
FT BamHI = G^GATCC
FT MboI = Sau3AI = ^GATC"
FT - 254..4801
FT /note="pUB110 789..4548..788 4548bp
FT MboI = Sau3AI = ^GATC
FT BamHI = G^GATCC"
FT - 4802..7213
FT /note="pUC9 254..2665 2412bp"
FT misc_binding 0..0
FT /note="MCS EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT misc_binding 0..0
FT /note="SIT unique BamHI-HindIII-SmaI-SalI-PstI-BglI-
FT TaqI-XbaI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ);
FT reporter gene"
FT CDS 0..0
FT /note="ANT E. coli kanamycin resistance gene (kan)"
XX
SQ Sequence 7213 BP; 1961 A; 1679 C; 1295 G; 2278 T; 0 other;
gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
ctgcaggtcg acggatccag ttgctcaaaa aaatctcggt cagatgttac tagcaactca
tttacaagaa cagcatcttt cctccgtttt tcttgtacct gttttttgtg attcaataat
ttctttgaca cgttcgttgt aatcaatatt tttatcattt ttcaaatcat aattttcacg
tgttcgctca tggtcaatat catcattcgt tctacttttt cgctctcttt gattatgaaa
ttgcatgcct tttagtccag ctgatttcac tttttgcatt ctacaaactg cataactcat
atgtaaatcg ctccttttta ggtggcacaa atgtgaggca ttttcgctct ttccggcaac
cacttccaag taaagtataa cacactatac tttatattca taaagtgtgt gctctgcgag
gctgtcggca gtgccgacca aaaccataaa acctttaaga cctttctttt ttttacgaga
aaaaagaaac aaaaaaacct gccctctgcc acctcagcaa aggggggttt tgctctcgtg
ctcgtttaaa aatcagcaag ggacaggtag tattttttga gaagatcact caaaaaatct
ccacctttaa acccttgcca atttttattt tgtccgtttt gtctagctta ccgaaagcca
gactcagcaa gaataaaatt tttattgtct ttcggttttc tagtgtaacg gacaaaacca
ctcaaaataa aaaagataca agagaggtct ctcgtatctt ttattcagca atcgcgcccg
attgctgaac agattaataa tagattttag ctttttattt gttgaaaaaa gctaatcaaa
ttgttgtcgg gatcaattac tgcaaagtct cgttcatccc accactgatc ttttaatgat
gtattggggt gcaaaatgcc caaaggctta atatgttgat ataattcatc aattccctct
acttcaatgc ggcaactagc agtaccagca ataaacgact ccgcacctgt acaaaccggt
gaatcattac tacgagagcg ccagcttcat cacttgcctc ccatagatga atccgaacct
cattacacat tagaactgcg aatccatctt catggtgaac caaagtgaaa cctagtttat
cgcaataaaa acctatactc tttttaatat ccccgactgg caatgccggg atagactgta
acattctcac gcataaaatc ccctttcatt ttctaatgta aatctattac cttattatta
attcaattcg ctcataatta atcctttttc ttattacgca aaatggcccg atttaagcac
accctttatt ccgttaatgc gccatgacag ccatgataat tactaatact aggagaagtt
aataaatacg taaccaacat gattaacaat tattagaggt catcgttcaa aatggtatgc
gttttgacac atccactata tatccgtgtc gttctgtcca ctcctgaatc ccattccaga
aattctctag cgattccaga agtttctcag agtcggaaag ttgaccagac attacgaact
ggcacagatg gtcataacct gaaggaagat ctgattgctt aactgcttca gttaagaccg
aagcgctcgt cgtataacag atgcgatgat gcagaccaat caacatggca cctgccattg
ctacctgtac agtcaaggat ggtagaaatg ttgtcggtcc ttgcacacga atattacgcc
atttgcctgc atattcaaac agctcttcta cgataagggc acaaatcgca tcgtggaacg
tttgggcttc taccgattta gcagtttgat acactttctc taagtatcca cctgaatcat
aaatcggcaa aatagagaaa aattgaccat gtgtaagcgg ccaatctgat tccacctgag
atgcataatc tagtagaatc tcttcgctat caaaattcac ttccaccttc cactcaccgg
ttgtccattc atggctgaac tctgcttcct ctgttgacat gacacacatc atctcaatat
ccgaataggg cccatcagtc tgacgaccaa gagagccata aacaccaata gccttaacat
catccccata tttatccaat attcgttcct taatttcatg aacaatcttc attctttctt
ctctagtcat tattattggt ccattcacta ttctcattcc cttttcagat aattttagat
ttgcttttct aaataagaat atttggagag caccgttctt attcagctat taataactcg
tcttcctaag catccttcaa tccttttaat aacaattata gcatctaatc ttcaacaaac
tggcccgttt gttgaactac tctttaataa aataattttt ccgttcccaa ttccacattg
caataataga aaatccatct tcatcggctt tttcgtcatc atctgtatga atcaaatcgc
cttcttctgt gtcatcaagg tttaattttt tatgtatttc ttttaacaaa ccaccatagg
agattaacct tttacggtgt aaaccttcct ccaaatcaga caaacgtttc aaattctttt
cttcatcatc ggtcataaaa tccgtatcct ttacaggata ttttgcagtt tcgtcaattg
ccgattgtat atccgattta tatttatttt tcggtcgaat catttgaact tttacatttg
gatcatagtc taatttcatt gcctttttcc aaaattgaat ccattgtttt tgattcacgt
agttttctgt attcttaaaa taagttggtt ccacacatac caatacatgc atgtgctgat
tataagaatt atctttatta tttattgtca cttccgttgc acgcataaaa ccaacaagat
ttttattaat ttttttatat tgcatcattc ggcgaaatcc ttgagccata tctgacaaac
tcttatttaa ttcttcgcca tcataaacat ttttaactgt taatgtgaga aacaaccaac
gaactgttgg cttttgttta ataacttcag caacaacctt ttgtgactga atgccatgtt
tcattgctct cctccagttg cacattggac aaagcctgga tttacaaaac cacactcgat
acaactttct ttcgcctgtt tcacgatttt gtttatactc taatatttca gcacaatctt
ttactctttc agccttttta aattcaagaa tatgcagaag ttcaaagtaa tcaacattag
cgattttctt ttctctccat ggtctcactt ttccactttt tgtcttgtcc actaaaaccc
ttgatttttc atctgaataa atgctactat taggacacat aatattaaaa gaaaccccca
tctatttagt tatttgttta gtcacttata actttaacag atggggtttt tctgtgcaac
caattttaag ggttttcaat actttaaaac acatacatac caacacttca acgcaccttt
cagcaactaa aataaaaatg acgttatttc tatatgtatc aagataagaa agaacaagtt
caaaaccatc aaaaaaagac accttttcag gtgctttttt tattttataa actcattccc
tgatctcgac ttcgttcttt ttttacctct cggttatgag ttagttcaaa ttcgttcttt
ttaggttcta aatcgtgttt ttcttggaat tgtgctgttt tatcctttac cttgtctaca
aaccccttaa aaacgttttt aaaggctttt aagccgtctg tacgttcctt aaggaattcc
ttagtgcttt catagattaa actcacatca cgctttaaat cgcttatttt agactttaaa
gacttgtttt cttcaagcaa ctcattataa tcatttacat tttcattaaa tcgctctaca
agaccactat atttttcttt aacttgccca tgttctttac ttaatttttt atattctctc
gccatatcag tactcatgag atttctaaca tgctgtttta acctatcgtt atctctcgca
gcagtcacta agtttttata atcacgctcc gatataacaa catttttggt tggtttcttt
tctgttttca ttatttcttt tcccaaacca aacatggact tttcacccgt tggcacttca
acacttttca tgtgtcgttt cgctggtact tctaaatctg atttaacttt atcgctataa
gcagtccatt catctttttt aactgctaaa tttttttcta gaaaatcaat ctctttttcc
aaagtttgtt ttttaaattt agctgtctca atatgtttac ggtcagagcc acgttcacca
cgcttcaact caaaaccctg ttttttcata tgctcgggga atttatcttg tagccataac
agttcttgac gattaaacac attttttcct tgcagttttc catcacgcat aggcacaaca
cctaaatgca tgtgaggggt ttgctcatca ttatgaactg ttgcataagc aatattttgc
ttgccatatc gttcggaaaa taatttataa ctttcctcaa aaaatcgttt ttgttctcct
ggatccccgg gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
aggaagcgga aga
//