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ID   PMK4       preliminary; circular DNA; SYN; 5575 BP.
XX
AC   ATCC37315;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/E.coli plasmid vector pMK4 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pMK3 from pUB110 & pUC9
RC   pMK4 from pC194 & pUC9
RA   Sullivan M.A., Yasbin R.E., Young F.E.;
RT   "New shuttle vectors for Bacillus subtilis and Escherichia coli
RT   which allow rapid detection of inserted fragments";
RL   Gene 29:21-26(1984).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   A Bacillus subtilis/E.coli shuttle vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pMK4)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli JM83)(Bacillus subtilis)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pC194)(pUC9)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pC194 Sau3A/MboI 2910bp 1..1, or 2005..2005
FT                   2. pUC9 MboI/Sau3A 2665bp 254..254, MCS
FT                   -> pMK4 5575bp"
FT   -               1..253
FT                   /note="pUC9 1..253 253bp
FT                   MboI = Sau3AI = ^GATC"
FT   -               254..3163
FT                   /note="pC194 1..2910 2910bp
FT                   MboI = Sau3AI = ^GATC"
FT   -               3164..5575bp
FT                   /note="pUC9 254..2665 2412bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-EcoRI-SmaI-SalI-PstI-Sau3A-
FT                   TaqI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
XX
SQ   Sequence 5575 BP; 1625 A; 1165 C; 1040 G; 1745 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
     ctgcaggtcg acggatcaat ttcttttaag taatctaaat ccccattttt taatttcttt
     ttagcctctt taaataatcc tgaataaact aatacctgtt tacctttaag tgatttataa
     aatgcatcaa agactttttg atttatttat taaataatca ctatctttac cagaatactt
     agccatttca tataattctt tattattatt ttgtcttatt ttttgaactt gaacttgtgt
     tatttctgaa atgcccgtta catcacgcca taaatctaac cattcttgtt ggctaatata
     atatctttta tctgtgaaat acgatttatt tactgcaatt aacacatgaa aatgaggatt
     ataatcatct ctttttttat tatatgtaat ctctaactta cgaacatatc cctttataac
     actacctact ttttttctct ttataagttt tctaaaagaa ttattataac gttttatttc
     attttctaat tcatcactca ttacattagg tgtagtcaaa gttaaaaaga taaactcctt
     tttctcttgc tgcttaatat attgcatcat caaagataaa cccaatgcat cttttctagc
     ttttctccaa gcacagacag gacaaaatcg atttttacaa gaattagctt tatataattt
     ctgtttttct aaagttttat cagctacaaa agacagaaat gtattgcaat cttcaactaa
     atccatttga ttctctccaa tatgacgttt aataaatttc tgaaatactt gatttctttg
     ttttttctca gtatactttt ccatgttata acacataaaa acaacttagt tttcacaaac
     tatgacaata aaaaaagttg ctttttcccc tttctatgta tgttttttac tagtcattta
     aaacgataca ttaataggta cgaaaaagca actttttttg cgcttaaaac cagtcatacc
     aataacttaa gggtaactag cctcgccggc aatagttacc cttattatca agataagaaa
     gaaaaggatt tttcgctacg ctcaaatcct ttaaaaaaac acaaaagacc acatttttta
     atgtggtctt tattcttcaa ctaaagcacc cattagttca acaaacgaaa attggataaa
     gtgggatatt tttaaaatat atatttatgt tacagtaata ttgactttta aaaaaggatt
     gattctaatg aagaaagcag acaagtaagc ctcctaaatt cactttagat aaaaatttag
     gaggcatatc aaatgaactt taataaaatt gatttagaca attggaagag aaaagagata
     tttaatcatt atttgaacca acaaacgact tttagtataa ccacagaaat tgatattagt
     gttttatacc gaaacataaa acaagaagga tataaatttt accctgcatt tattttctta
     gtgacaaggg tgataaactc aaatacagct tttagaactg gttacaatag cgacggagag
     ttaggttatt gggataagtt agagccactt tatacaattt ttgatggtgt atctaaaaca
     ttctctggta tttggactcc tgtaaagaat gacttcaaag agttttatga tttatacctt
     tctgatgtag agaaatataa tggttcgggg aaattgtttc ccaaaacacc tatacctgaa
     aatgcttttt ctctttctat tattccatgg acttcattta ctgggtttaa cttaaatatc
     aataataata gtaattacct tctacccatt attacagcag gaaaattcat taataaaggt
     aattcaatat atttaccgct atctttacag gtacatcatt ctgtttgtga tggttatcat
     gcaggattgt ttatgaactc tattcaggaa ttgtcagata ggcctaatga ctggctttta
     taatatgaga taatgccgac tgtacttttt acagtcggtt ttctaatgtc actaacctgc
     cccgttagtt gaagaaggtt tttatattac agctccagat ccatatcctt ctttttctga
     accgacttct cctttttcgc ttctttattc caattgcttt attgacgttg agcctcggaa
     cccttaacaa tcccaaaact tgtcgaatgg tcggcttaat agctcacgct atgccgacat
     tcgtctgcaa gtttagttaa gggttcttct caacgcacaa taaattttct cggcataaat
     gcgtggtcta atttttattt ttaataacct tgatagcaaa aaatgccatt ccaatacaaa
     accacatacc tataatcgat aaccacataa cagtcataaa accactcctt tttaacaaac
     tttatcacaa gaaatattta aattttaaat gcctttattt tgaattttaa ggggcatttt
     aaagatttag gggtaaatca tatagtttta tgcctaaaaa cctacagaag cttttaaaaa
     gcaaatatga gccaaataaa tatattctaa ttctacaaac aaaaatttga gcaaattcag
     tgtcgatttt ttaagacact gcccagttac atgcaaatta aaattttcat gattttttat
     agttcctaac agggttaaaa tttgtataac gaaagtataa tgtttatata acgttagtat
     aataaagcat tttaacatta tacttttgat aatcgtttat cgtcgtcatc acaataactt
     ttaaaatact cgtgcataat tcacgctgac ctcccaataa ctacatggtg ttatcgggag
     gtcagctgtt agcacttata ttttgttatt gttcttcctc gatttcgtct atcattttgt
     gattaatttc tcttttttct tgttctgtta agtcataaag ttcactagct aaatactctt
     tttgtttcca aatataaaaa atttgataga tatattacgg ttggatcccc gggaattcac
     tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaga
//