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ID   PML2       preliminary; circular DNA; SYN; 2970 BP.
XX
AC   IG0089;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pML2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pOT from pBR322 & SV40
RC   pJY1 from pBR322 & SV40
RC   pJYM from pBR322 & SV40
RC   pOK3 from pBR322 & SV40
RC   pDAx from pBR322 & SV40
RC   pSV01 from pBR322 & SV40
RC   pSV03 from pSV01
RC   pML, pML-1 from pJYM
RC   [pML2 from pML-1]
RC   pML-RIIG from pML-1 & SV40 ori
RC   pCAT2-polylinker from pSV2cat
RC   [pML-RIIG-ChTK from pML-RIIG & chicken tk gene]
RA   Lusky M., Botchan M.R.;
RT   "Inhibition of SV40 replication in simian cells by specific pBR322
RT   DNA sequences";
RL   Nature 293:79-81(1981).
XX
RN   [2]
RC   pTK2 from HSV tk gene
RC   pOT from pBR322 & SV40 early region
RC   pOT-TK from pOT & pTK2
RA   Hanahan D., Lane D., Lipsich L., Wigler M., Botchan M.;
RT   "Characteristics of an SV40-plasmid recombinant and its movement
RT   into and out of the genome of a murine cell";
RL   Cell 21:127-139(1980).
XX
RN   [3]
RC   pSV01 from pBR322 & SV40 ori/early promoter/T antigen
RC   pSV04 from pSV01 & pNB1
RC   DL1, DL4, DL5, DL12, DL22, DL23 from pSV04
RA   Myers R.M., Tjian R.;
RT   "Construction and analysis of simian virus 40 origins defective in
RT   tumor antigen binding and DNA replication";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:6491-6495(1980).
XX
CC   derived from pBR322 by deletion of poison sequences (1092..2484).
CC   NM (pML2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR (pML2)
CC   OF (pSB1[bacteria] from pML2 and pSV2-cat)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   2. SV40 BamHI 5243bp 2534..2534
FT                   -> pJY1 9604bp
FT                   1. pJY1 remove 1393bp, pBR322 1092..2485 poison seq
FT                   \ 8211bp [pBR322 1095..2485]
FT                   -> pJYM 8211bp
FT                   1. pJYM remove BamHI-BamHI 5243bp, SV40
FT                   -> pML-1 2968bp
FT                   1. pML-1 remove, 2968bp-
FT                   -> pML-2 2900bp [2971bp]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1902..2762)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2970 BP; 670 A; 807 C; 753 G; 740 T; 0 other;
     agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga
     gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     ggccctttcg tcttcaagaa ttctcatgtt tgacagctta tcatcgataa gctttaatgc
     ggtagtttat cacagttaaa ttgctaacgc
//