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ID   PML3LACP29 preliminary; circular DNA; SYN; 6862 BP.
XX
AC   A10361;
XX
DT   01-FEB-1995 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid pML3/lacOP29 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6862
RC   pML3 from pBR322
RC   pML3/lacOP29 from pML3 & lacOP
RA   Bujard H., Lanzer M.;
RT   "High repressible sequence for control of expression";
RL   Unpublished (1986).
RL   European Patent No. 0303925-A 8 dated 22-FEB-1989.
RL   F. HOFFMANN-LA ROCHE AG.
XX
CC   NCBI gi: 490683
CC   NM (pML3/lacOP29)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   -> pML3/lacOP29 6862bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 6862 BP; 1589 A; 1882 C; 1801 G; 1590 T; 0 other;
     aaatagtaca taatggattt ccttacgcga aatacgggca gacatggcct gcccggttat
     tattattttt gacaccagac caactggtaa tggtagcgac cggcgctcag ctggaattcc
     gccgatactg acgggctcca ggagtcgtcg ccaccaatcc ccatatggaa accgtcgata
     ttcagccatg tgccttcttc cgcgtgcagc agatggcgat ggctggtttc catcagttgc
     tgttgactgt agcggctgat gttgaactgg aagtcgccgc gccactggtg tgggccataa
     ttcaattcgc gcgtcccgca gcgcagaccg ttttcgctcg ggaagacgta cggggtatac
     atgtctgaca atggcagatc ccagcggtca aaacaggcgg cagtaaggcg gtcgggatag
     ttttcttgcg gccctaatcc gagccagttt acccgctctg ctacctgcgc cagctggcag
     ttcaggccaa tccgcgccgg atgcggtgta tcgctcgcca cttcaacatc aacggtaatc
     gccatttgac cactaccatc aatccggtag gttttccggc tgataaataa ggttttcccc
     tgatgctgcc acgcgtgagc ggtcgtaatc agcaccgcat cagcaagtgt atctgccgtg
     cactgcaaca acgctgcttc ggcctggtaa tggcccgccg ccttccagcg ttcgacccag
     gcgttagggt caatgcgggt cgcttcactt acgccaatgt cgttatccag cggtgcacgg
     gtgaactgat cgcgcagcgg cgtcagcagt tgttttttat cgccaatcca catctgtgaa
     agaaagcctg actggcggtt aaattgccaa cgcttattac ccagctcgat gcaaaaatcc
     atttcgctgg tggtcagatg cgggatggcg tgggacgcgg cggggagcgt cacactgagg
     ttttccgcca gacgccactg ctgccaggcg ctgatgtgcc cggcttctga ccatgcggtc
     gcgttcggtt gcactacgcg tactgtgagc cagagttgcc cggcgctctc cggctgcggt
     agttcaggca gttcaatcaa ctgtttacct tgtggagcga catccagagg cacttcaccg
     cttgccagcg gcttaccatc cagcgccacc atccagtgca ggagctcgtt atcgctatga
     cggaacaggt attcgctggt cacttcgatg gtttgcccgg ataaacggaa ctggaaaaac
     tgctgctggt gttttgcttc cgtcagcgct ggatgcggcg tgcggtcggc aaagaccaga
     ccgttcatac agaactggcg atcgttcggc gtatcgccaa aatcaccgcc gtaagccgac
     cacgggttgc cgttttcatc atatttaatc agcgactgat ccacccagtc ccagacgaag
     ccgccctgta aacggggata ctgacgaaac gcctgccagt atttagcgaa accgccaaga
     ctgttaccca tcgcgtgggc gtattcgcaa aggatcagcg ggcgcgtctc tccaggtagc
     gaaagccatt ttttgatgga ccatttcggc acagccggga agggctggtc ttcatccacg
     cgcgcgtaca tcgggcaaat aatatcggtg gccgtggtgt cggctccgcc gccttcatac
     tgcaccgggc gggaaggatc gacagatttg atccagcgat acagcgcgtc gtgattagcg
     ccgtggcctg attcattccc cagcgaccag atgatcacac tcgggtgatt acgatcgcgc
     tgcaccattc gcgttacgcg ttcgctcatc gccggtagcc agcgcggatc atcggtcaga
     cgattcattg gcaccatgcc gtgggtttca atattggctt catccaccac atacaggccg
     tagcggtcgc acagcgtgta ccacagcgga tggttcggat aatgcgaaca gcgcacggcg
     ttaaagttgt tctgcttcat cagcaggata tcctgcacca tcgtctgctc atccatgacc
     tgaccatgca gaggatgatg ctcgtgacgg ttaacgcctc gaatcagcaa cggcttgccg
     ttcagcagca gcagaccatt ttcaatccgc acctcgcgga aaccgacatc gcaggcttct
     gcttcaatca gcgtgccgtc ggcggtgtgc agttcaacca ccgcacgata gagattcggg
     atttcggcgc tccacagttt cgggttttcg acgttcagac gtagtgtgac gcgatcggca
     taaccaccac gctcatcgat aatttcaccg ccgaaaggcg cggtgccgct ggcgacctgc
     gtttcaccct gccataaaga aactgttacc cgtaggtagt cacgcaactc gccgcacatc
     tgaacttcag cctccagtac agcgcggctg aaatcatcat taaagcgagt ggcaacatgg
     aaatcgctga tttgtgtagt cggtttatgc agcaacgaga cgtcacggaa aatgccgctc
     atccgccaca tatcctgatc ttccagataa ctgccgtcac tccaacgcag caccatcacc
     gcgaggcggt tttctccggc gcgtaaaaat gcgctcaggt caaattcaga cggcaaacga
     ctgtcctggc cgtaaccgac ccagcgcccg ttgcaccaca gatgaaacgc cgagttaacg
     ccatcaaaaa taattcgcgt ctggccttcc tgtagccagc tttcatcaac attaaatgtg
     agcgagtaac aacccgtcgg attctccgtg ggaacaaacg gcggattgac cgtaatggga
     taggttacgt tggtgtagat gggcgcatcg taaccgtgca tctgccagtt tgaggggacg
     acgacagtat cggcctcagg aagatcgcac tccagccagc tttccggcac cgcttctggt
     gccggaaacc aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     tccgtaatca tggtcatagc tgtttcctgt gtgaattctg tgtgaaattg ttatccgctc
     acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     gtgagctaac tcacattaat tgcgttgcct cgaggtacct tataaacgca gaaaggccca
     cccgaaggtg agccagttaa cgtcgacaat tcgcgctaac ttacattaat tgcgttgcgc
     tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
     gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
     cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
     acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
     cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
     cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
     actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
     ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
     ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
     gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
     agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
     cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
     tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
     cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
     cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
     cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     tttgcaccat tcgatggtgt caacgtaaat gcatgccgct tcgccttcgc gcgcgaattg
     tcgactagag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
     cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     cacgaggccc tttcgtcttc aa
//