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ID   PMLB1034   preliminary; circular DNA; SYN; 6189 BP.
XX
AC   IG0090; ATCC37222; K01450;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMLB1034 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCV1 from pBR322 & linker
RC   pSKS101 from pUC7 & Tn5
RC   pSKS114 from pUC7 & Tn9
RC   pMC1827, pMC1828 from pMC1403 & pSKS101
RC   pMC1843 from pMC1827
RC   pMLB1034 from pMC1403
RC   pMC1844 from pMLB1034 & pUC7
RC   pSKS104 from pMC1403 & pUC7
RC   pSKS105 from pMC1403 & pUC8
RC   pSKS106 from pMC1403 & pUC9
RC   pSKS107 from pSKS105
RC   pFR10 from pSKS106 & pCV1
RC   pFR97 from pSKS104 & pFR10
RC   pFR98 from pSKS105 & pFR10
RC   pFR109 from pSKS106 & pFR10
RC   plasmid from pMC279 & pMC1403
RC   plasmid2 from plasmid & pSKS101
RC   pMC1871 from plasmid2 & pMC1843
RA   Shapira S.K., Chou J., Richaud F.V., Casadaban M.J.;
RT   "New versatile plasmid vectors for expression of hybrid proteins
RT   coded by a cloned gene fused to lacZ gene sequences encoding an
RT   enzymatically active carboxy-terminal portion of beta-galactosidase";
RL   Gene 25:71-82(1983).
XX
RN   [2]
RC   [pMLB1031 from pMC871 & pBR322]
RC   pMLB1034 from pMC871 & pBR322
RC   pORF5 from pMLB1034 & pBH20
RC   [lambda spc2 from lambda]
RC   pNF3041, pNF3033 from lambda spc2 & pUC8
RC   pNF3042 from pNF3033
RC   pNF3043 from pNF3041
RC   pJS600 from pNF3041
RC   pJS1800 from pNF3033
RC   pJS2200 from pNF3033
RC   pJS1000 from pNF3041 & pORF5
RA   Shultz J., Silhavy T.J., Berman M.L., Fiil N., Emr S.D.;
RT   "A previously unidentified gene in the spc operon of Escherichia
RT   coli K12 specifies a component of the protein export machinery";
RL   Cell 31:227-235(1982).
XX
RN   [3]
RC   pMLB1034 from pMC871 & pBR322; [6259 bp]
RA   Berman M.L.;
RT   "Vectors for constructing hybrid genes";
RL   Biotechniques 1:178-183(1983).
XX
RN   [4]
RC   [from none]
RA   Silhavy T.J., Berman M.L., Enquist L.W.;
RT   ;
RL   Experiments with Gene Fusion 0:0-0(1984).
RL   Silhavy T.J., Berman M.L., Enquist L.W.,
RL   Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
XX
CC   ATCC size is 7200 bp.
CC   The cloning sites in this vector are 5' to a promoterless
CC   beta-galactosidase gene.The insert must provide the promoter, the
CC   ribosome binding site, and/or the ATG translation initiation codon
CC   for activity to be expressed. [4]
CC   A plasmid vector for screening promoters by determining
CC   beta-galactosidase activity (lacZ). [4]
CC   Escherichia coli MC1000 = F- araD139 delta(ara, leu)7697
CC   delta(lac)chi74 galU- galK- rpsL.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pMLB1034)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli MC1000)(E.coli MC1061)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pMC1403)
CC   BR (pMC1843)(pMC1844)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. ColE1 6646bp
FT                   2. E. coli, lacZ
FT                   -> pMC81
FT                   1. pMC81
FT                   2. pACYC177 3941bp
FT                   -> pMC275
FT                   1. pMC275
FT                   -> pMC874
FT                   1. pBR322 4361bp
FT                   2. E. coli, lacZ
FT                   -> pMC489
FT                   1. pMB9 5300bp
FT                   2. pMC489
FT                   -> pMC511
FT                   1. pMC874
FT                   2. pMC511
FT                   -> pMC871
FT                   1. pMC871 EcoRI-AvaI 3317bp,
FT                   \ 9th codon of lacZ to 208 of lacY
FT                   2. pBR322 remove EcoRI-AvaI 1427bp 4360..4361..1426,
FT                   \ ori/amp 2934bp
FT                   -> pMLB1034 6189bp"
FT   misc_binding    85..85
FT                   /note="SIT AvaI"
FT   rep_origin      197..197
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1870..2730)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1269..1269
FT                   /note="SIT PstI"
FT   CDS             2951..6012
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   misc_binding    2951..2967
FT                   /note="MCS EcoRI-XmaI-AvaI-SmaI-BamHI"
FT   misc_binding    3778..3778
FT                   /note="SIT ClaI"
FT   misc_binding    4293..4293
FT                   /note="SIT AvaI"
FT   misc_binding    4301..4301
FT                   /note="SIT BclI"
FT   misc_binding    4891..4891
FT                   /note="SIT SstI"
FT   misc_binding    5736..5736
FT                   /note="SIT AvaI"
FT   misc_binding    5736..5736
FT                   /note="SIT AvaI"
FT   misc_feature    join(6013..6189,1..85)
FT                   /note="GEN E. coli lacY gene"
XX
SQ   Sequence 6189 BP; 1459 A; 1640 C; 1630 G; 1460 T; 0 other;
     ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
     actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
     tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
     tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
     attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
     cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
     ctcgtttcat cggtatcatt acccccatga acagaaattc ccccttacac ggaggcatca
     agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt
     aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc
     gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg
     tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt cccggggatc
     ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
     cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
     caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta
     cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
     atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact
     cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc
     cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg
     tgctgcgttg gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca
     ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg
     ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg
     gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg
     ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc
     gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc
     tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct
     gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc
     cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg
     atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc
     gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt
     atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg
     atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc
     gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc
     acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag
     gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg
     aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg
     gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg
     gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct
     gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt
     acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct
     ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc
     agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg
     ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag
     tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc
     cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat
     ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg
     tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt
     gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga
     tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac
     cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg
     aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag
     atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa
     ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
     ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc
     tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
     accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc
     cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc
     cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga
     tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg
     gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaatttc
     agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg
     gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca
     caaacttttg gatgttcggt ttattctttt tcttttactt ttttatcatg ggagcctact
     tcccgttttt cccgatttgg ctacatgaca tcaaccatat cagcaaaagt gatacgggta
     ttatttttgc cgctatttct ctgttctcgc tattattcca accgctgttt ggtctgcttt
     ctgacaaac
//