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ID   PMMB190    preliminary; circular DNA; SYN; 9064 BP.
XX
AC   M37846; M28993; ATCC37807;
XX
DT   20-MAR-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Broad host range/E.coli plasmid vector pMMB190 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-9064
RC   [pMMB98 from pMMB series]
RC   pMMB190 from pUC8 & pMMB66EH
RC   pMMB206 from pUC8CM & pMMB190
RC   pMMB207 from pMMB66EH & pMMB206
RC   pMMB208 from pMMB66HE & pMMB206
RC   pMMB213 from pAC1 & pMMB206
RC   pMMB271 from pMMB98 & pMMB206
RC   pMMB274 from pCS13 & pMMB213
RC   pMMB276 from pCS13 & pMMB213
RC   pMMB277 from pMMB206
RC   pMMB278 from pMMB190
RC   pMMB311 from pMMB271
RC   pMMB313 from pMMB213 & pMMB311
RA   Morales V.M., Baeckman A., Bagdasarian M.;
RT   "A series of wide-host-range low-copy-number vectors that allow
RT   direct screening for recombinants";
RL   Gene 97:39-47(1991).
XX
RN   [2]
RC   pMMB190
RA   Morales V.M.;
RT   ;
RL   Submitted (21-AUG-1990) to GenBank on computer-readable media by:
RL   Morales V.M., Natl. Res. Council of Canada, Plant Biotech. Inst.,
RL   Saskatoon SK S7N OW9, Canada.
XX
RN   [3]
RC   pUC8CM from pUC8 & cat gene
RA   Buckley K.J.;
RT   "Regulation and expression of the phiX174 lysis gene";
RL   Thesis [U. California San Diego] 0:0-0(1985).
XX
RN   [4]
RC   pCS13 from pBR322 & ptsM gene
RC   pNW6 from pCS13 & pBR322
RC   pNW10 from pCS13 & pDR720
RC   pNW23 from pCS13 & pUC18
RC   pNW8, pNW15 from pCS13 & pUC19
RC   pNW18 from pCS13 & pUC8
RC   pNW24 from pCS13 & pUC18
RC   pNW11, pNW14 from pCS13 & pYEJ001
RA   Williams N., Fox D.K., Shea C., Roseman S.;
RT   "Pel, the protein that permits lambda DNA penetration of Escherichia
RT   coli, is encoded by a gene in ptsM and is required for mannose
RT   utilization by the phosphotransferase system";
RL   Proc. Natl. Acad. Sci. U.S.A. 83:8934-8938(1986).
XX
RN   [5]
RC   pAC1 from pBBo & placIq
RC   pAJC178, pAJC264, pAJC352 from pAC1
RA   Boulain J.C., Charbit A., Hofnung M.;
RT   "Mutagenesis by random linker insertion into the lamB gene of
RT   Escherichia coli K-12";
RL   Mol. Gen. Genet. 205:339-348(1986).
XX
RN   [6]
RC   F' hisG9424 from F' factor
RC   F' hisG9424::Tn10 from F' hisG9424 & Tn10
RC   pNK75 from pBR333 & hisOGD genes
RC   pNK81 from pNK75 & F' hisG9424::Tn10
RC   pNK217 from pNK81
RC   pNK289 from pNK81
RC   pNK474 from pBR333 & Tn10
RC   pNK569 from pBR333 & Tn10
RC   pNK579 from pBR333 & tet gene
RC   pNK861 from pBR333 & Tn10
RC   pNK862 from pBR333 & Tn10
RC   pNK1022 from pNK81
RC   lambda 370 from lambda & Tn10
RC   lambda 561 from lambda & Tn10
RC   lambda 634 from lambda & Tn10
RC   lambda 775 from lambda & Tn10
RC   lambda 809 from lambda & Tn10
RC   lambda 840 from lambda & Tn10
RC   lambda 939 from lambda & Tn10
RC   lambda 940 from lambda & Tn10
RC   lambda 1045 from lambda & Tn10
RC   lambda 1046 from lambda & Tn10
RC   lambda 1048 from lambda & Tn10
RC   lambda 1052 from lambda & Tn10
RC   lambda 1098 from lambda & Tn10
RC   lambda 1104 from lambda & Tn10
RC   lambda 1105 from lambda & Tn10
RC   from pOX38 & pNK569
RC   from pOX38 & pNK861
RC   from pOX38 & pNK862
RA   Way J.C., Davis M.A., Morisato D., Roberts D.E., Kleckner N.;
RT   "New Tn10 derivatives for transposon mutagenesis and for
RT   construction of lacZ operon fusions by transposition";
RL   Gene 32:369-379(1984).
XX
RN   [7]
RC   pBBo from lamB gene
RA   Bouges-Bocquet B., Villarroya H., Hofnung M.;
RT   "Linker mutagenesis in the gene of an outer membrane protein of
RT   Escherichia coli, LamB";
RL   J. Cell. Biochem. 24:217-228(1984).
XX
RN   [8]
RC   pBR333 from pBR322
RA   Backman K.;
RT   ;
RL   Unpublished (1984).
XX
CC   Contains sequences encoding lacIq, a tandem tac lacUV5 promoter
CC   region, and lacZalpha to allow direct screening by inactivation of
CC   alpha-complementation.  Shows a low level of expression without
CC   induction. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--9.1; EcoRV--5.3, 3.8; SspI--4.9, 1.8, 1.6, 1.0;
CC   EcoRI/MluI--8.2, 0.9. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pMMB190)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli TB1)(E.coli DH5alphaF')(E.coli)(Gram-positive bacteria)
CC   HO (broad host range)
CC   CP (low)
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA ()
CC   BR (pMMB66EH)
CC   OF (pMMB206)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 HaeII-HaeII 424bp 5..429, lacZalpha
FT                   mung bean nuclease:mung bean nuclease,408bp lacZalpha
FT                   HaeII-EcoRI 226bp 5..231, lacZalpha
FT                   2. pMMB66EH HindIII-EcoRI 8777bp 29..8806
FT                   blunt end:
FT                   -> pMMB190 9064bp"
FT   misc_feature    join(9047..9063,1..181)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')
FT                   alpha peptide"
FT   promoter        638..668
FT                   /note="PRO E. coli bla gene"
FT   CDS             708..1568
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      2006..2400
FT                   /note="ORI E. coli IncQ plasmid origin of vegetative
FT                   replication oriV"
FT   CDS             complement(2426..2710)
FT                   /note="GEN E. coli mobC gene
FT                   (conjugational DNA transfer)"
FT   rep_origin      2663..2820
FT                   /note="ORI E. coli IncQ plasmid origin of conjugative
FT                   DNA transfer oriT"
FT   promoter        2702..2732
FT                   /note="PRO E. coli promoter P1"
FT   promoter        2721..2751
FT                   /note="PRO E. coli promoter P3"
FT   CDS             2909..5038
FT                   /note="GEN E. coli repB/mobA gene (primase)"
FT   CDS             3657..4070
FT                   /note="GEN E. coli mobB gene
FT                   (conjugational DNA transfer)"
FT   CDS             4067..5038
FT                   /note="GEN E. coli repB' gene (primase)"
FT   promoter        5040..5071
FT                   /note="PRO E. coli promoter P4"
FT   CDS             5099..5311
FT                   /note="GEN E. coli E gene (function unknown)"
FT   CDS             5313..5519
FT                   /note="GEN E. coli cac gene
FT                   (regulates repA and repC gene expression)"
FT   CDS             5549..6388
FT                   /note="GEN E. coli repA gene (DNA helicase)"
FT   CDS             6375..7226
FT                   /note="GEN E. coli repC gene (replication initiator
FT                   protein)"
FT   promoter        7387..7417
FT                   /note="PRO E. coli promoter P5"
FT   CDS             complement(7503..8586)
FT                   /note="REP E. coli lacI repressor gene
FT                   (contains stop codons)"
FT   promoter        8620..8649
FT                   /note="PRO E. coli lacIq gene"
FT   promoter        8893..8921
FT                   /note="PRO E. coli tac (trp and lac)"
FT   promoter        8975..9004
FT                   /note="PRO E. coli lacUV5"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
XX
SQ   Sequence 9064 BP; 1962 A; 2653 C; 2692 G; 1757 T; 0 other;
     ttcccgggga tccgtcgacc tgcagccaag cttggcactg gccgtcgttt tacaacgtcg
     tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     cagctggcta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     aatggcgatg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
     gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
     cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
     ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
     gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
     gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     attggtaact gtcagaccaa gtttactcat atatacttta gattgatttc tgaaagcgac
     caggtgctcg gcgtggcaag actcgcagcg aacccgtaga aagccatgct ccagccgccc
     gcattggaga aattcttcaa attcccgttg cacatagccc ggcaattcct ttccctgctc
     tgccataagc gcagcgaatg ccgggtaata ctcgtcaacg atctgataga gaagggtttg
     ctcgggtcgg tggctctggt aacgaccagt atcccgatcc cggctggccg tcctggccgc
     cacatgaggc atgttccgcg tccttgcaat actgtgttta catacagtct atcgcttagc
     ggaaagttct tttaccctca gccgaaatgc ctgccgttgc tagacattgc cagccagtgc
     ccgtcactcc cgtactaact gtcacgaacc cctgcaataa ctgtcacgcc cccctgcaat
     aactgtcacg aacccctgca ataactgtca cgcccccaaa cctgcaaacc cagcaggggc
     gggggctggc ggggtgttgg aaaaatccat ccatgattat ctaagaataa tccactaggc
     gcggttatca gcgcccttgt ggggcgctgc tgcccttgcc caatatgccc ggccagaggc
     cggatagctg gtctattcgc tgcgctaggc tacacaccgc cccaccgctg cgcggcaggg
     ggaaaggcgg gcaaagcccg ctaaacccca caccaaaccc cgcagaaata cgctggagcg
     cttttagccg ctttagcggc ctttccccct acccgaaggg tgggggcgcg tgtgcagccc
     cgcagggcct gtctcggtcg atcattcagc ccggctcatc cttctggcgt ggcggcagac
     cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc cattgccgcc atgagccgat
     cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat ggcccccacc agcaccttgc
     gccttgtttc gttcttgcgc tcttgctgct gttcccttgc ccgctcccgc tgaatttcgg
     cattgattcg cgctcgttgt tcttcgagct tggccagccg atccgccgcc ttgttgctcc
     ccttaaccat cttgacaccc cattgttaat gtgctgtctc gtaggctatc atggaggcac
     agcggcggca atcccgaccc tactttgtag gggagggcgc acttaccggt ttctcttcga
     gaaactggcc taacggccac ccttcgggcg gtgcgctctc cgagggccat tgcatggagc
     cgaaaagcaa aagcaacagc gaggcagcat ggcgatttat caccttacgg cgaaaaccgg
     cagcaggtcg ggcggccaat cggccagggc caaggccgac tacatccagc gcgaaggcaa
     gtatgcccgc gacatggatg aagtcttgca cgccgaatcc gggcacatgc cggagttcgt
     cgagcggccc gccgactact gggatgctgc cgacctgtat gaacgcgcca atgggcggct
     gttcaaggag gtcgaatttg ccctgccggt cgagctgacc ctcgaccagc agaaggcgct
     ggcgtccgag ttcgcccagc acctgaccgg tgccgagcgc ctgccgtata cgctggccat
     ccatgccggt ggcggcgaga acccgcactg ccacctgatg atctccgagc ggatcaatga
     cggcatcgag cggcccgccg ctcagtggtt caagcggtac aacggcaaga ccccggagaa
     gggcggggca cagaagaccg aagcgctcaa gcccaaggca tggcttgagc agacccgcga
     ggcatgggcc gaccatgcca accgggcatt agagcgggct ggccacgacg cccgcattga
     ccacagaaca cttgaggcgc agggcatcga gcgcctgccc ggtgttcacc tggggccgaa
     cgtggtggag atggaaggcc ggggcatccg caccgaccgg gcagacgtgg ccctgaacat
     cgacaccgcc aacgcccaga tcatcgactt acaggaatac cgggaggcaa tagaccatga
     acgcaatcga cagagtgaag aaatccagag gcatcaacga gttagcggag cagatcgaac
     cgctggccca gagcatggcg acactggccg acgaagcccg gcaggtcatg agccagacca
     agcaggccag cgaggcgcag gcggcggagt ggctgaaagc ccagcgccag acaggggcgg
     catgggtgga gctggccaaa gagttgcggg aggtagccgc cgaggtgagc agcgccgcgc
     agagcgcccg gagcgcgtcg cgggggtggc actggaagct atggctaacc gtgatgctgg
     cttccatgat gcctacggtg gtgctgctga tcgcatcgtt gctcttgctc gacctgacgc
     cactgacaac cgaggacggc tcgatctggc tgcgcttggt ggcccgatga agaacgacag
     gactttgcag gccataggcc gacagctcaa ggccatgggc tgtgagcgct tcgatatcgg
     cgtcagggac gcacccaccg gccagatgat gaaccgggaa tggtcagccg ccgaagtgct
     ccagaacacg ccatggctca agcggatgaa tgcccagggc aatgacgtgt atatcaggcc
     cgccgagcag gagcggcatg gtctggtgct ggtggacgac ctcagcgagt ttgacctgga
     tgacatgaaa gccgagggcc gggagcctgc cctggtagtg gaaaccagcc cgaagaacta
     tcaggcatgg gtcaaggtgg ccgacgccgc aggcggtgaa cttcgggggc agattgcccg
     gacgctggcc agcgagtacg acgccgaccc ggccagcgcc gacagccgcc actatggccg
     cttggcgggc ttcaccaacc gcaaggacaa gcacaccacc cgcgccggtt atcagccgtg
     ggtgctgctg cgtgaatcca agggcaagac cgccaccgct ggcccggcgc tggtgcagca
     ggctggccag cagatcgagc aggcccagcg gcagcaggag aaggcccgca ggctggccag
     cctcgaactg cccgagcggc agcttagccg ccaccggcgc acggcgctgg acgagtaccg
     cagcgagatg gccgggctgg tcaagcgctt cggtcatgac ctcagcaagt gcgactttat
     cgccgcgcag aagctggcca gccggggccg cagtgccgag gaaatcggca aggccatggc
     cgaggccagc ccagcgctgg cagagcgcaa gcccggccac gaagcggatt acatcgagcg
     caccgtcagc aaggtcatgg gtctgcccag cgtccagctt gcgcgggccg agctggcacg
     ggcaccggca ccccgccagc gaggcatgga caggggcggg ccagatttca gcatgtagtg
     cttgcgttgg tactcacgcc tgttatacta tgagtactca cgcacagaag ggggttttat
     ggaatacgaa aaaagcgctt cagggtcggt ctacctgatc aaaagtgaca agggctattg
     gttgcccggt ggctttggtt atacgtcaaa caaggccgag gctggccgct tttcagtcgc
     tgatatggcc agccttaacc ttgacggctg caccttgtcc ttgttccgcg aagacaagcc
     tttcggcccc ggcaagtttc tcggtgactg atatgaaaga ccaaaaggac aagcagaccg
     gcgacctgct ggccagccct gacgctgtac gccaagcgcg atatgccgag cgcatgaagg
     ccaaagggat gcgtcagcgc aagttctggc tgaccgacga cgaatacgag gcgctgcgcg
     agtgcctgga agaactcaga gcggcgcagg gcgggggtag tgaccccgcc agcgcctaac
     caccaactgc ctgcaaagga ggcaatcaat ggctacccat aagcctatca atattctgga
     ggcgttcgca gcagcgccgc caccgctgga ctacgttttg cccaacatgg tggccggtac
     ggtcggggcg ctggtgtcgc ccggtggtgc cggtaaatcc atgctggccc tgcaactggc
     cgcacagatt gcaggcgggc cggatctgct ggaggtgggc gaactgccca ccggcccggt
     gatctacctg cccgccgaag acccgcccac cgccattcat caccgcctgc acgcccttgg
     ggcgcacctc agcgccgagg aacggcaagc cgtggctgac ggcctgctga tccagccgct
     gatcggcagc ctgcccaaca tcatggcccc ggagtggttc gacggcctca agcgcgccgc
     cgagggccgc cgcctgatgg tgctggacac gctgcgccgg ttccacatcg aggaagaaaa
     cgccagcggc cccatggccc aggtcatcgg tcgcatggag gccatcgccg ccgataccgg
     gtgctctatc gtgttcctgc accatgccag caagggcgcg gccatgatgg gcgcaggcga
     ccagcagcag gccagccggg gcagctcggt actggtcgat aacatccgct ggcagtccta
     cctgtcgagc atgaccagcg ccgaggccga ggaatggggt gtggacgacg accagcgccg
     gttcttcgtc cgcttcggtg tgagcaaggc caactatggc gcaccgttcg ctgatcggtg
     gttcaggcgg catgacggcg gggtgctcaa gcccgccgtg ctggagaggc agcgcaagag
     caagggggtg ccccgtggtg aagcctaaga acaagcacag cctcagccac gtccggcacg
     acccggcgca ctgtctggcc cccggcctgt tccgtgccct caagcggggc gagcgcaagc
     gcagcaagct ggacgtgacg tatgactacg gcgacggcaa gcggatcgag ttcagcggcc
     cggagccgct gggcgctgat gatctgcgca tcctgcaagg gctggtggcc atggctgggc
     ctaatggcct agtgcttggc ccggaaccca agaccgaagg cggacggcag ctccggctgt
     tcctggaacc caagtgggag gccgtcaccg ctgaatgcca tgtggtcaaa ggtagctatc
     gggcgctggc aaaggaaatc ggggcagagg tcgatagtgg tggggcgctc aagcacatac
     aggactgcat cgagcgcctt tggaaggtat ccatcatcgc ccagaatggc cgcaagcggc
     aggggtttcg gctgctgtcg gagtacgcca gcgacgaggc ggacgggcgc ctgtacgtgg
     ccctgaaccc cttgatcgcg caggccgtca tgggtggcgg ccagcatgtg cgcatcagca
     tggacgaggt gcgggcgctg gacagcgaaa ccgcccgcct gctgcaccag cggctgtgtg
     gctggatcga ccccggcaaa accggcaagg cttccataga taccttgtgc ggctatgtct
     ggccgtcaga ggccagtggt tcgaccatgc gcaagcgccg caagcgggtg cgcgaggcgt
     tgccggagct ggtcgcgctg ggctggacgg taaccgagtt cgcggcgggc aagtacgaca
     tcacccggcc caaggcggca ggctgacccc ccccactcta ttgtaaacaa gacattttta
     tcttttatat tcaatggctt attttcctgc taattggtaa taccatgaaa aataccatgc
     tcagaaaagg cttaacaata ttttgaaaaa ttgcctactg agcgctgccg cacagctcca
     taggccgctt tcctggcttt gcttccagat gtatgctctt ctgctcccga acgccagcaa
     gacgtagccc agcgcgtcgg ccagcttgca attcgcgcta acttacatta attgcgttgc
     gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
     gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
     tccacgtggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
     aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca
     gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
     tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
     cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
     gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
     tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg
     agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
     tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
     gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
     ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
     ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
     acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
     tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     ttttgcacca ttcgatggtg tcaacgtaaa tgccgcttcg ccttcgcgcg cgaattgcaa
     gctgatccgg gcttatcgac tgcacggtgc accaatgctt ctggcgtcag gctgccatcg
     gaagctgtgg tatggctgtg caggtcgtaa atcactgcat aattcgtgtc gctcaaggcg
     cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg caaatattct
     gaaatgagct gttgacaatt aatcatcggc tcgtataatg tgtggaattg tgagcggata
     acaatttcac acaggaaact aggcacccca ggctttacac tttatgcttc cggctcgtat
     gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     cgaa
//