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ID   PMMB206    preliminary; circular DNA; SYN; 9311 BP.
XX
AC   M37847; M28994; ATCC37808;
XX
DT   20-MAR-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Broad host range/E.coli plasmid vector pMMB206 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-9311
RC   [pMMB98 from pMMB series]
RC   pMMB190 from pUC8 & pMMB66EH
RC   pMMB206 from pUC8CM & pMMB190
RC   pMMB207 from pMMB66EH & pMMB206
RC   pMMB208 from pMMB66HE & pMMB206
RC   pMMB213 from pAC1 & pMMB206
RC   pMMB271 from pMMB98 & pMMB206
RC   pMMB274 from pCS13 & pMMB213
RC   pMMB276 from pCS13 & pMMB213
RC   pMMB277 from pMMB206
RC   pMMB278 from pMMB190
RC   pMMB311 from pMMB271
RC   pMMB313 from pMMB213 & pMMB311
RA   Morales V.M., Baeckman A., Bagdasarian M.;
RT   "A series of wide-host-range low-copy-number vectors that allow
RT   direct screening for recombinants";
RL   Gene 97:39-47(1991).
XX
RN   [2]
RC   pMMB206
RA   Morales V.M.;
RT   ;
RL   Submitted (21-AUG-1990) to GenBank on computer-readable media by:
RL   Morales V.M., Natl. Res. Council of Canada, Plant Biotech. Inst.,
RL   Saskatoon SK S7N OW9, Canada.
XX
CC   Contains sequences encoding lacIq, a tandem tac lacUV5 promoter
CC   region, and lacZalpha to allow direct screening by inactivation of
CC   alpha-complementation.  Shows a low level of expression without
CC   induction. [1]
CC   Sequencing demonstrated that there are 3 stop codons in frame with
CC   the truncated bla gene, terminating translation but permitting
CC   continued transcription and expression of the cat gene. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--9.3; EcoRV--5.8, 3.6; SspI--3.9, 1.8, 1.6, 1.2, 0.9;
CC   EcoRI/MluI--8.4, 0.9. (ATCC staff)
CC   Medium is 1675 LB plus chloramphenicol (10 ug/ml).
CC   NM (pMMB206)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli TB1)(E.coli DH5alphaF')(E.coli)(Gram-positive bacteria)
CC   HO (broad host range)
CC   CP (low)
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pMMB190)
CC   BR (pMMB66EH)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 2665bp, MCS
FT                   2. Tn9 EcoRI, cat gene/#V00622
FT                   mutagenesis, [no EcoRI in cat]
FT                   Tn9 TaqI-TaqI 766bp 215..-..988, modified cat gene
FT                   -> pUC8CM 3431bp
FT                   1. pMMB190 XmnI 9064bp 1694..1694
FT                   2. pUC8CM BanI-HaeII 1064bp 131..-..429, cat gene
FT                   blunt end:blunt end
FT                   -> pMMB206 9311bp"
FT   CDS             join(9295..9311,1..181)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')
FT                   alpha peptide"
FT   promoter        638..668
FT                   /note="PRO E. coli bla gene"
FT   CDS             708..941
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp); truncated"
FT   promoter        1168..1194
FT                   /note="PRO E. coli cat gene"
FT   CDS             1261..1920
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   rep_origin      2234..2647
FT                   /note="ORI E. coli IncQ plasmid origin of vegetative
FT                   replication oriV"
FT   CDS             complement(2673..2957)
FT                   /note="GEN E. coli mobC gene
FT                   (conjugational DNA transfer)"
FT   rep_origin      2910..3067
FT                   /note="ORI E. coli IncQ plasmid origin of conjugative
FT                   DNA transfer oriT"
FT   promoter        2968..2998
FT                   /note="PRO E. coli promoter P3"
FT   CDS             3156..5285
FT                   /note="GEN E. coli repB/mobA gene (primase) "
FT   CDS             3904..4317
FT                   /note="GEN E. coli mobB gene
FT                   (conjugational DNA transfer)"
FT   CDS             4314..5285
FT                   /note="GEN E. coli repB' gene (primase)"
FT   promoter        5287..5318
FT                   /note="PRO E. coli promoter P4"
FT   CDS             5346..5558
FT                   /note="GEN E. coli E gene (function unknown)"
FT   CDS             5560..5766
FT                   /note="GEN E. coli cac gene
FT                   (regulates repA and repC gene expression)"
FT   CDS             5796..6635
FT                   /note="GEN E. coli repA gene (DNA helicase)"
FT   CDS             6622..7473
FT                   /note="GEN E. coli repC gene (replication initiator
FT                   protein)"
FT   promoter        7634..7664
FT                   /note="PRO E. coli promoter P5"
FT   CDS             complement(7750..8833)
FT                   /note="REP E. coli lacIq repressor gene
FT                   (contains stop codons)"
FT   promoter        9140..9168
FT                   /note="PRO E. coli tac (trp and lac)"
FT   promoter        9223..9251
FT                   /note="PRO E. coli lacUV5"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
XX
SQ   Sequence 9311 BP; 2042 A; 2692 C; 2726 G; 1851 T; 0 other;
     ttcccgggga tccgtcgacc tgcagccaag cttggcactg gccgtcgttt tacaacgtcg
     tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     cagctggcta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     aatggcgatg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
     gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
     cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
     ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
     gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
     gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgtgatg
     tccggcggtg cttttgccgt tacgcaccac cccgtcagta gctgaacagg agggacagct
     gatagaaaca gaagccactg gagcaagcac ctcaaaaaca ccatcataca ctaaatcagt
     aagttggcag catcacccga cgcactttgc gccgaataaa tacctgtgac ggaagatcac
     ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt
     tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata
     agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
     atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
     cacattcttg cccgcctgat gaatgctcat ccggaattac gtatggcaat gaaagacggt
     gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
     gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
     tttttttaag gcagttattg tcttcaaatt cccgttgcac atagcccggc aattcctttc
     cctgctctgc cataagcgca gcgaatgccg ggtaatactc gtcaacgatc tgatagagaa
     gggtttgctc gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg ctggccgtcc
     tggccgccac atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat acagtctatc
     gcttagcgga aagttctttt accctcagcc gaaatgcctg ccgttgctag acattgccag
     ccagtgcccg tcactcccgt actaactgtc acgaacccct gcaataactg tcacgccccc
     ctgcaataac tgtcacgaac ccctgcaata actgtcacgc ccccaaacct gcaaacccag
     caggggcggg ggctggcggg gtgttggaaa aatccatcca tgattatcta agaataatcc
     actaggcgcg gttatcagcg cccttgtggg gcgctgctgc ccttgcccaa tatgcccggc
     cagaggccgg atagctggtc tattcgctgc gctaggctac acaccgcccc accgctgcgc
     ggcaggggga aaggcgggca aagcccgcta aaccccacac caaaccccgc agaaatacgc
     tggagcgctt ttagccgctt tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt
     gcagccccgc agggcctgtc tcggtcgatc attcagcccg gctcatcctt ctggcgtggc
     ggcagaccga acaaggcgcg gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg
     agccgatcct ccggccactc gctgctgttc accttggcca aaatcatggc ccccaccagc
     accttgcgcc ttgtttcgtt cttgcgctct tgctgctgtt cccttgcccg ctcccgctga
     atttcggcat tgattcgcgc tcgttgttct tcgagcttgg ccagccgatc cgccgccttg
     ttgctcccct taaccatctt gacaccccat tgttaatgtg ctgtctcgta ggctatcatg
     gaggcacagc ggcggcaatc ccgaccctac tttgtagggg agggcgcact taccggtttc
     tcttcgagaa actggcctaa cggccaccct tcgggcggtg cgctctccga gggccattgc
     atggagccga aaagcaaaag caacagcgag gcagcatggc gatttatcac cttacggcga
     aaaccggcag caggtcgggc ggccaatcgg ccagggccaa ggccgactac atccagcgcg
     aaggcaagta tgcccgcgac atggatgaag tcttgcacgc cgaatccggg cacatgccgg
     agttcgtcga gcggcccgcc gactactggg atgctgccga cctgtatgaa cgcgccaatg
     ggcggctgtt caaggaggtc gaatttgccc tgccggtcga gctgaccctc gaccagcaga
     aggcgctggc gtccgagttc gcccagcacc tgaccggtgc cgagcgcctg ccgtatacgc
     tggccatcca tgccggtggc ggcgagaacc cgcactgcca cctgatgatc tccgagcgga
     tcaatgacgg catcgagcgg cccgccgctc agtggttcaa gcggtacaac ggcaagaccc
     cggagaaggg cggggcacag aagaccgaag cgctcaagcc caaggcatgg cttgagcaga
     cccgcgaggc atgggccgac catgccaacc gggcattaga gcgggctggc cacgacgccc
     gcattgacca cagaacactt gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg
     ggccgaacgt ggtggagatg gaaggccggg gcatccgcac cgaccgggca gacgtggccc
     tgaacatcga caccgccaac gcccagatca tcgacttaca ggaataccgg gaggcaatag
     accatgaacg caatcgacag agtgaagaaa tccagaggca tcaacgagtt agcggagcag
     atcgaaccgc tggcccagag catggcgaca ctggccgacg aagcccggca ggtcatgagc
     cagaccaagc aggccagcga ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca
     ggggcggcat gggtggagct ggccaaagag ttgcgggagg tagccgccga ggtgagcagc
     gccgcgcaga gcgcccggag cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg
     atgctggctt ccatgatgcc tacggtggtg ctgctgatcg catcgttgct cttgctcgac
     ctgacgccac tgacaaccga ggacggctcg atctggctgc gcttggtggc ccgatgaaga
     acgacaggac tttgcaggcc ataggccgac agctcaaggc catgggctgt gagcgcttcg
     atatcggcgt cagggacgca cccaccggcc agatgatgaa ccgggaatgg tcagccgccg
     aagtgctcca gaacacgcca tggctcaagc ggatgaatgc ccagggcaat gacgtgtata
     tcaggcccgc cgagcaggag cggcatggtc tggtgctggt ggacgacctc agcgagtttg
     acctggatga catgaaagcc gagggccggg agcctgccct ggtagtggaa accagcccga
     agaactatca ggcatgggtc aaggtggccg acgccgcagg cggtgaactt cgggggcaga
     ttgcccggac gctggccagc gagtacgacg ccgacccggc cagcgccgac agccgccact
     atggccgctt ggcgggcttc accaaccgca aggacaagca caccacccgc gccggttatc
     agccgtgggt gctgctgcgt gaatccaagg gcaagaccgc caccgctggc ccggcgctgg
     tgcagcaggc tggccagcag atcgagcagg cccagcggca gcaggagaag gcccgcaggc
     tggccagcct cgaactgccc gagcggcagc ttagccgcca ccggcgcacg gcgctggacg
     agtaccgcag cgagatggcc gggctggtca agcgcttcgg tcatgacctc agcaagtgcg
     actttatcgc cgcgcagaag ctggccagcc ggggccgcag tgccgaggaa atcggcaagg
     ccatggccga ggccagccca gcgctggcag agcgcaagcc cggccacgaa gcggattaca
     tcgagcgcac cgtcagcaag gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc
     tggcacgggc accggcaccc cgccagcgag gcatggacag gggcgggcca gatttcagca
     tgtagtgctt gcgttggtac tcacgcctgt tatactatga gtactcacgc acagaagggg
     gttttatgga atacgaaaaa agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg
     gctattggtt gcccggtggc tttggttata cgtcaaacaa ggccgaggct ggccgctttt
     cagtcgctga tatggccagc cttaaccttg acggctgcac cttgtccttg ttccgcgaag
     acaagccttt cggccccggc aagtttctcg gtgactgata tgaaagacca aaaggacaag
     cagaccggcg acctgctggc cagccctgac gctgtacgcc aagcgcgata tgccgagcgc
     atgaaggcca aagggatgcg tcagcgcaag ttctggctga ccgacgacga atacgaggcg
     ctgcgcgagt gcctggaaga actcagagcg gcgcagggcg ggggtagtga ccccgccagc
     gcctaaccac caactgcctg caaaggaggc aatcaatggc tacccataag cctatcaata
     ttctggaggc gttcgcagca gcgccgccac cgctggacta cgttttgccc aacatggtgg
     ccggtacggt cggggcgctg gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc
     aactggccgc acagattgca ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg
     gcccggtgat ctacctgccc gccgaagacc cgcccaccgc cattcatcac cgcctgcacg
     cccttggggc gcacctcagc gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc
     agccgctgat cggcagcctg cccaacatca tggccccgga gtggttcgac ggcctcaagc
     gcgccgccga gggccgccgc ctgatggtgc tggacacgct gcgccggttc cacatcgagg
     aagaaaacgc cagcggcccc atggcccagg tcatcggtcg catggaggcc atcgccgccg
     ataccgggtg ctctatcgtg ttcctgcacc atgccagcaa gggcgcggcc atgatgggcg
     caggcgacca gcagcaggcc agccggggca gctcggtact ggtcgataac atccgctggc
     agtcctacct gtcgagcatg accagcgccg aggccgagga atggggtgtg gacgacgacc
     agcgccggtt cttcgtccgc ttcggtgtga gcaaggccaa ctatggcgca ccgttcgctg
     atcggtggtt caggcggcat gacggcgggg tgctcaagcc cgccgtgctg gagaggcagc
     gcaagagcaa gggggtgccc cgtggtgaag cctaagaaca agcacagcct cagccacgtc
     cggcacgacc cggcgcactg tctggccccc ggcctgttcc gtgccctcaa gcggggcgag
     cgcaagcgca gcaagctgga cgtgacgtat gactacggcg acggcaagcg gatcgagttc
     agcggcccgg agccgctggg cgctgatgat ctgcgcatcc tgcaagggct ggtggccatg
     gctgggccta atggcctagt gcttggcccg gaacccaaga ccgaaggcgg acggcagctc
     cggctgttcc tggaacccaa gtgggaggcc gtcaccgctg aatgccatgt ggtcaaaggt
     agctatcggg cgctggcaaa ggaaatcggg gcagaggtcg atagtggtgg ggcgctcaag
     cacatacagg actgcatcga gcgcctttgg aaggtatcca tcatcgccca gaatggccgc
     aagcggcagg ggtttcggct gctgtcggag tacgccagcg acgaggcgga cgggcgcctg
     tacgtggccc tgaacccctt gatcgcgcag gccgtcatgg gtggcggcca gcatgtgcgc
     atcagcatgg acgaggtgcg ggcgctggac agcgaaaccg cccgcctgct gcaccagcgg
     ctgtgtggct ggatcgaccc cggcaaaacc ggcaaggctt ccatagatac cttgtgcggc
     tatgtctggc cgtcagaggc cagtggttcg accatgcgca agcgccgcaa gcgggtgcgc
     gaggcgttgc cggagctggt cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag
     tacgacatca cccggcccaa ggcggcaggc tgaccccccc cactctattg taaacaagac
     atttttatct tttatattca atggcttatt ttcctgctaa ttggtaatac catgaaaaat
     accatgctca gaaaaggctt aacaatattt tgaaaaattg cctactgagc gctgccgcac
     agctccatag gccgctttcc tggctttgct tccagatgta tgctcttctg ctcccgaacg
     ccagcaagac gtagcccagc gcgtcggcca gcttgcaatt cgcgctaact tacattaatt
     gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt
     caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
     caagcggtcc acgtggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
     gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca
     acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
     accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
     gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
     tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
     agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
     tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
     ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
     atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
     acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
     ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
     atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
     tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
     accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
     actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
     cgaaaggttt tgcaccattc gatggtgtca acgtaaatgc cgcttcgcct tcgcgcgcga
     attgcaagct gatccgggct tatcgactgc acggtgcacc aatgcttctg gcgtcaggca
     gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct
     caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa
     atattctgaa atgagctgtt gacaattaat catcggctcg tataatgtgt ggaattgtga
     gcggataaca atttcacaca ggaaactagg caccccaggc tttacacttt atgcttccgg
     ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     atgattacga a
//