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ID   PMMB207    preliminary; circular DNA; SYN; 9075 BP.
XX
AC   M37847; M28994; M37852; M37846; M28993; M37851; M37850; M37849;
AC   M37848; ATCC37809;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Broad host range/E.coli plasmid vector pMMB207 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pMMB98 from pMMB series]
RC   pMMB190 from pUC8 & pMMB66EH
RC   pMMB206 from pUC8CM & pMMB190
RC   pMMB207 from pMMB66EH & pMMB206
RC   pMMB208 from pMMB66HE & pMMB206
RC   pMMB213 from pAC1 & pMMB206
RC   pMMB271 from pMMB98 & pMMB206
RC   pMMB274 from pCS13 & pMMB213
RC   pMMB276 from pCS13 & pMMB213
RC   pMMB277 from pMMB206
RC   pMMB278 from pMMB190
RC   pMMB311 from pMMB271
RC   pMMB313 from pMMB213 & pMMB311
RA   Morales V.M., Baeckman A., Bagdasarian M.;
RT   "A series of wide-host-range low-copy-number vectors that allow
RT   direct screening for recombinants";
RL   Gene 97:39-47(1991).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Constructed by replacing the MluI/XmnI fragment of pMMB206 (taclacUV5
CC   promoter, M13mp8 polylinker, lacZalpha) with the MluI/XmnI fragment of
CC   pMMB67EH (tac promoter, M13mp18 polylinker without lacZalpha).
CC   pMMB207 (ATCC 37809) and pMMB208 (ATCC 37810) differ in the
CC   orientation of the multiple cloning site with pMMB207 having the EcoRI
CC   site near the promoter and pMMB208 having the HindIII site nearer.
CC   Does not have a start codon at a suitable distance after the
CC   ribosome-binding site, so translation will be efficient only if the
CC   insert contains an RBS followed appropriately by a start codon.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--9.1; EcoRV--5.3, 3.9; SspI--4.1, 1.8, 1.6, 1.0, 0.7;
CC   EcoRI/MluI--8.3, 0.8. (ATCC staff)
CC   Medium is 1675 LB plus chloramphenicol (10 ug/ml).
CC   NM (pMMB207)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli TB1)(broad host range)(E.coli)(E.coli DH5alphaF')
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pMMB67EH)(pMMB206)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMMB67EH MluI-XmnI 813bp
FT                   \ 8074..8805..51bp..29..59
FT                   2. pMMB206 remove MluI-XmnI 1049bp 8477..9311..215,
FT                   \ 8262bp [944bp]
FT                   -> pMMB207 9072bp"
FT   -               1..8262
FT                   /note="pMMB206 215..8476 8262bp
FT                   MluI = A^CGCGT"
FT   -               8263..9075
FT                   /note="pMMB67EH 8046..8828..30 813bp
FT                   XmnI = GAANN^NNTTC"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-PstI-
FT                   SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-
FT                   SalI-PstI-SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli IncQ plasmid RSF1010 oriT and oriV"
FT   promoter        0..0
FT                   /note="PRO E. coli tac"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
XX
SQ   Sequence 9075 BP; 1988 A; 2620 C; 2672 G; 1795 T; 0 other;
     ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc
     ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc
     gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa
     ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
     cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
     gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat
     acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     gagttttcgc cccgaagaac gtgatgtccg gcggtgcttt tgccgttacg caccaccccg
     tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc aagcacctca
     aaaacaccat catacactaa atcagtaagt tggcagcatc acccgacgca ctttgcgccg
     aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg
     ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag
     gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
     gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     aattacgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattgtctt caaattcccg
     ttgcacatag cccggcaatt cctttccctg ctctgccata agcgcagcga atgccgggta
     atactcgtca acgatctgat agagaagggt ttgctcgggt cggtggctct ggtaacgacc
     agtatcccga tcccggctgg ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc
     aatactgtgt ttacatacag tctatcgctt agcggaaagt tcttttaccc tcagccgaaa
     tgcctgccgt tgctagacat tgccagccag tgcccgtcac tcccgtacta actgtcacga
     acccctgcaa taactgtcac gcccccctgc aataactgtc acgaacccct gcaataactg
     tcacgccccc aaacctgcaa acccagcagg ggcgggggct ggcggggtgt tggaaaaatc
     catccatgat tatctaagaa taatccacta ggcgcggtta tcagcgccct tgtggggcgc
     tgctgccctt gcccaatatg cccggccaga ggccggatag ctggtctatt cgctgcgcta
     ggctacacac cgccccaccg ctgcgcggca gggggaaagg cgggcaaagc ccgctaaacc
     ccacaccaaa ccccgcagaa atacgctgga gcgcttttag ccgctttagc ggcctttccc
     cctacccgaa gggtgggggc gcgtgtgcag ccccgcaggg cctgtctcgg tcgatcattc
     agcccggctc atccttctgg cgtggcggca gaccgaacaa ggcgcggtcg tggtcgcgtt
     caaggtacgc atccattgcc gccatgagcc gatcctccgg ccactcgctg ctgttcacct
     tggccaaaat catggccccc accagcacct tgcgccttgt ttcgttcttg cgctcttgct
     gctgttccct tgcccgctcc cgctgaattt cggcattgat tcgcgctcgt tgttcttcga
     gcttggccag ccgatccgcc gccttgttgc tccccttaac catcttgaca ccccattgtt
     aatgtgctgt ctcgtaggct atcatggagg cacagcggcg gcaatcccga ccctactttg
     taggggaggg cgcacttacc ggtttctctt cgagaaactg gcctaacggc cacccttcgg
     gcggtgcgct ctccgagggc cattgcatgg agccgaaaag caaaagcaac agcgaggcag
     catggcgatt tatcacctta cggcgaaaac cggcagcagg tcgggcggcc aatcggccag
     ggccaaggcc gactacatcc agcgcgaagg caagtatgcc cgcgacatgg atgaagtctt
     gcacgccgaa tccgggcaca tgccggagtt cgtcgagcgg cccgccgact actgggatgc
     tgccgacctg tatgaacgcg ccaatgggcg gctgttcaag gaggtcgaat ttgccctgcc
     ggtcgagctg accctcgacc agcagaaggc gctggcgtcc gagttcgccc agcacctgac
     cggtgccgag cgcctgccgt atacgctggc catccatgcc ggtggcggcg agaacccgca
     ctgccacctg atgatctccg agcggatcaa tgacggcatc gagcggcccg ccgctcagtg
     gttcaagcgg tacaacggca agaccccgga gaagggcggg gcacagaaga ccgaagcgct
     caagcccaag gcatggcttg agcagacccg cgaggcatgg gccgaccatg ccaaccgggc
     attagagcgg gctggccacg acgcccgcat tgaccacaga acacttgagg cgcagggcat
     cgagcgcctg cccggtgttc acctggggcc gaacgtggtg gagatggaag gccggggcat
     ccgcaccgac cgggcagacg tggccctgaa catcgacacc gccaacgccc agatcatcga
     cttacaggaa taccgggagg caatagacca tgaacgcaat cgacagagtg aagaaatcca
     gaggcatcaa cgagttagcg gagcagatcg aaccgctggc ccagagcatg gcgacactgg
     ccgacgaagc ccggcaggtc atgagccaga ccaagcaggc cagcgaggcg caggcggcgg
     agtggctgaa agcccagcgc cagacagggg cggcatgggt ggagctggcc aaagagttgc
     gggaggtagc cgccgaggtg agcagcgccg cgcagagcgc ccggagcgcg tcgcgggggt
     ggcactggaa gctatggcta accgtgatgc tggcttccat gatgcctacg gtggtgctgc
     tgatcgcatc gttgctcttg ctcgacctga cgccactgac aaccgaggac ggctcgatct
     ggctgcgctt ggtggcccga tgaagaacga caggactttg caggccatag gccgacagct
     caaggccatg ggctgtgagc gcttcgatat cggcgtcagg gacgcaccca ccggccagat
     gatgaaccgg gaatggtcag ccgccgaagt gctccagaac acgccatggc tcaagcggat
     gaatgcccag ggcaatgacg tgtatatcag gcccgccgag caggagcggc atggtctggt
     gctggtggac gacctcagcg agtttgacct ggatgacatg aaagccgagg gccgggagcc
     tgccctggta gtggaaacca gcccgaagaa ctatcaggca tgggtcaagg tggccgacgc
     cgcaggcggt gaacttcggg ggcagattgc ccggacgctg gccagcgagt acgacgccga
     cccggccagc gccgacagcc gccactatgg ccgcttggcg ggcttcacca accgcaagga
     caagcacacc acccgcgccg gttatcagcc gtgggtgctg ctgcgtgaat ccaagggcaa
     gaccgccacc gctggcccgg cgctggtgca gcaggctggc cagcagatcg agcaggccca
     gcggcagcag gagaaggccc gcaggctggc cagcctcgaa ctgcccgagc ggcagcttag
     ccgccaccgg cgcacggcgc tggacgagta ccgcagcgag atggccgggc tggtcaagcg
     cttcggtcat gacctcagca agtgcgactt tatcgccgcg cagaagctgg ccagccgggg
     ccgcagtgcc gaggaaatcg gcaaggccat ggccgaggcc agcccagcgc tggcagagcg
     caagcccggc cacgaagcgg attacatcga gcgcaccgtc agcaaggtca tgggtctgcc
     cagcgtccag cttgcgcggg ccgagctggc acgggcaccg gcaccccgcc agcgaggcat
     ggacaggggc gggccagatt tcagcatgta gtgcttgcgt tggtactcac gcctgttata
     ctatgagtac tcacgcacag aagggggttt tatggaatac gaaaaaagcg cttcagggtc
     ggtctacctg atcaaaagtg acaagggcta ttggttgccc ggtggctttg gttatacgtc
     aaacaaggcc gaggctggcc gcttttcagt cgctgatatg gccagcctta accttgacgg
     ctgcaccttg tccttgttcc gcgaagacaa gcctttcggc cccggcaagt ttctcggtga
     ctgatatgaa agaccaaaag gacaagcaga ccggcgacct gctggccagc cctgacgctg
     tacgccaagc gcgatatgcc gagcgcatga aggccaaagg gatgcgtcag cgcaagttct
     ggctgaccga cgacgaatac gaggcgctgc gcgagtgcct ggaagaactc agagcggcgc
     agggcggggg tagtgacccc gccagcgcct aaccaccaac tgcctgcaaa ggaggcaatc
     aatggctacc cataagccta tcaatattct ggaggcgttc gcagcagcgc cgccaccgct
     ggactacgtt ttgcccaaca tggtggccgg tacggtcggg gcgctggtgt cgcccggtgg
     tgccggtaaa tccatgctgg ccctgcaact ggccgcacag attgcaggcg ggccggatct
     gctggaggtg ggcgaactgc ccaccggccc ggtgatctac ctgcccgccg aagacccgcc
     caccgccatt catcaccgcc tgcacgccct tggggcgcac ctcagcgccg aggaacggca
     agccgtggct gacggcctgc tgatccagcc gctgatcggc agcctgccca acatcatggc
     cccggagtgg ttcgacggcc tcaagcgcgc cgccgagggc cgccgcctga tggtgctgga
     cacgctgcgc cggttccaca tcgaggaaga aaacgccagc ggccccatgg cccaggtcat
     cggtcgcatg gaggccatcg ccgccgatac cgggtgctct atcgtgttcc tgcaccatgc
     cagcaagggc gcggccatga tgggcgcagg cgaccagcag caggccagcc ggggcagctc
     ggtactggtc gataacatcc gctggcagtc ctacctgtcg agcatgacca gcgccgaggc
     cgaggaatgg ggtgtggacg acgaccagcg ccggttcttc gtccgcttcg gtgtgagcaa
     ggccaactat ggcgcaccgt tcgctgatcg gtggttcagg cggcatgacg gcggggtgct
     caagcccgcc gtgctggaga ggcagcgcaa gagcaagggg gtgccccgtg gtgaagccta
     agaacaagca cagcctcagc cacgtccggc acgacccggc gcactgtctg gcccccggcc
     tgttccgtgc cctcaagcgg ggcgagcgca agcgcagcaa gctggacgtg acgtatgact
     acggcgacgg caagcggatc gagttcagcg gcccggagcc gctgggcgct gatgatctgc
     gcatcctgca agggctggtg gccatggctg ggcctaatgg cctagtgctt ggcccggaac
     ccaagaccga aggcggacgg cagctccggc tgttcctgga acccaagtgg gaggccgtca
     ccgctgaatg ccatgtggtc aaaggtagct atcgggcgct ggcaaaggaa atcggggcag
     aggtcgatag tggtggggcg ctcaagcaca tacaggactg catcgagcgc ctttggaagg
     tatccatcat cgcccagaat ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg
     ccagcgacga ggcggacggg cgcctgtacg tggccctgaa ccccttgatc gcgcaggccg
     tcatgggtgg cggccagcat gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg
     aaaccgcccg cctgctgcac cagcggctgt gtggctggat cgaccccggc aaaaccggca
     aggcttccat agataccttg tgcggctatg tctggccgtc agaggccagt ggttcgacca
     tgcgcaagcg ccgcaagcgg gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga
     cggtaaccga gttcgcggcg ggcaagtacg acatcacccg gcccaaggcg gcaggctgac
     cccccccact ctattgtaaa caagacattt ttatctttta tattcaatgg cttattttcc
     tgctaattgg taataccatg aaaaatacca tgctcagaaa aggcttaaca atattttgaa
     aaattgccta ctgagcgctg ccgcacagct ccataggccg ctttcctggc tttgcttcca
     gatgtatgct cttctgctcc cgaacgccag caagacgtag cccagcgcgt cggccagctt
     gcaattcgcg ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     accgcctggc cctgagagag ttgcagcaag cggtccacgt ggtttgcccc agcaggcgaa
     aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg gtgtcaacgt
     aaatgccgct tcgccttcgc gcgcgaattg caagctgatc cgggcttatc gactgcacgg
     tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg
     taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg
     cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc
     ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagaattcg
     agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggctgttttg
     gcggatgaga gaaga
//