Return to this vector's summary.
ID PMMB208 preliminary; circular DNA; SYN; 9089 BP.
XX
AC M37847; M28994; M37852; M37846; M28993; M37851; M37850; M37849;
AC M37848; ATCC37810;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Broad host range/E.coli plasmid vector pMMB208 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC [pMMB98 from pMMB series]
RC pMMB190 from pUC8 & pMMB66EH
RC pMMB206 from pUC8CM & pMMB190
RC pMMB207 from pMMB66EH & pMMB206
RC pMMB208 from pMMB66HE & pMMB206
RC pMMB213 from pAC1 & pMMB206
RC pMMB271 from pMMB98 & pMMB206
RC pMMB274 from pCS13 & pMMB213
RC pMMB276 from pCS13 & pMMB213
RC pMMB277 from pMMB206
RC pMMB278 from pMMB190
RC pMMB311 from pMMB271
RC pMMB313 from pMMB213 & pMMB311
RA Morales V.M., Baeckman A., Bagdasarian M.;
RT "A series of wide-host-range low-copy-number vectors that allow
RT direct screening for recombinants";
RL Gene 97:39-47(1991).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC pMMB207 (ATCC 37809) and pMMB208 (ATCC 37810) differ in the
CC orientation of the multiple cloning site with pMMB207 having the EcoRI
CC site near the promoter and pMMB208 having the HindIII site nearer.
CC Does not have a start codon at a suitable distance after the
CC ribosome-binding site, so translation will be efficient only if the
CC insert contains an RBS followed appropriately by a start codon.
CC Restriction digests of the clone give the following sizes (kb):
CC EcoRI--9.1; EcoRV--5.3, 3.9; SspI--4.1, 1.8, 1.6, 1.0, 0.7;
CC EcoRI/MluI--8.3, 0.8. (ATCC staff)
CC Medium is 1675 LB plus chloramphenicol (10 ug/ml).
CC NM (pMMB208)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli TB1)(broad host range)(E.coli)(E.coli DH5alphaF')
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pMMB67HE)(pMMB206)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pMMB67HE MluI-XmnI 827bp
FT \ 8074..8821..6..51bp..36..59
FT \ [802bp]
FT 2. pMMB206 remove MluI-XmnI 1049bp 8477..9311..215,
FT \ 8262bp [944bp]
FT -> pMMB208 9082bp"
FT - 1..8262
FT /note="pMMB206 215..8476 8262bp
FT MluI = A^CGCGT"
FT - 8263..9089
FT /note="pMMB67HE 8107..8842..91 827bp
FT XmnI = GAANN^NNTTC"
FT misc_binding 0..0
FT /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-
FT KpnI-SacI-EcoRI"
FT misc_binding 0..0
FT /note="SIT unique HindIII-SphI-PstI-SalI-XbaI-BamHI-
FT SmaI-KpnI-SacI-EcoRI"
FT rep_origin 0..0
FT /note="ORI E. coli IncQ plasmid RSF1010 oriT and oriV"
FT promoter 0..0
FT /note="PRO E. coli tac"
FT CDS 0..0
FT /note="ANT E. coli chloramphenicol acetyltransferase
FT gene (cat); chloramphenicol resistance gene (cmr/cml)"
XX
SQ Sequence 9089 BP; 1993 A; 2622 C; 2673 G; 1801 T; 0 other;
ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc
ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc
gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa
ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat
acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
gagttttcgc cccgaagaac gtgatgtccg gcggtgcttt tgccgttacg caccaccccg
tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc aagcacctca
aaaacaccat catacactaa atcagtaagt tggcagcatc acccgacgca ctttgcgccg
aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg
ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag
gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
aattacgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattgtctt caaattcccg
ttgcacatag cccggcaatt cctttccctg ctctgccata agcgcagcga atgccgggta
atactcgtca acgatctgat agagaagggt ttgctcgggt cggtggctct ggtaacgacc
agtatcccga tcccggctgg ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc
aatactgtgt ttacatacag tctatcgctt agcggaaagt tcttttaccc tcagccgaaa
tgcctgccgt tgctagacat tgccagccag tgcccgtcac tcccgtacta actgtcacga
acccctgcaa taactgtcac gcccccctgc aataactgtc acgaacccct gcaataactg
tcacgccccc aaacctgcaa acccagcagg ggcgggggct ggcggggtgt tggaaaaatc
catccatgat tatctaagaa taatccacta ggcgcggtta tcagcgccct tgtggggcgc
tgctgccctt gcccaatatg cccggccaga ggccggatag ctggtctatt cgctgcgcta
ggctacacac cgccccaccg ctgcgcggca gggggaaagg cgggcaaagc ccgctaaacc
ccacaccaaa ccccgcagaa atacgctgga gcgcttttag ccgctttagc ggcctttccc
cctacccgaa gggtgggggc gcgtgtgcag ccccgcaggg cctgtctcgg tcgatcattc
agcccggctc atccttctgg cgtggcggca gaccgaacaa ggcgcggtcg tggtcgcgtt
caaggtacgc atccattgcc gccatgagcc gatcctccgg ccactcgctg ctgttcacct
tggccaaaat catggccccc accagcacct tgcgccttgt ttcgttcttg cgctcttgct
gctgttccct tgcccgctcc cgctgaattt cggcattgat tcgcgctcgt tgttcttcga
gcttggccag ccgatccgcc gccttgttgc tccccttaac catcttgaca ccccattgtt
aatgtgctgt ctcgtaggct atcatggagg cacagcggcg gcaatcccga ccctactttg
taggggaggg cgcacttacc ggtttctctt cgagaaactg gcctaacggc cacccttcgg
gcggtgcgct ctccgagggc cattgcatgg agccgaaaag caaaagcaac agcgaggcag
catggcgatt tatcacctta cggcgaaaac cggcagcagg tcgggcggcc aatcggccag
ggccaaggcc gactacatcc agcgcgaagg caagtatgcc cgcgacatgg atgaagtctt
gcacgccgaa tccgggcaca tgccggagtt cgtcgagcgg cccgccgact actgggatgc
tgccgacctg tatgaacgcg ccaatgggcg gctgttcaag gaggtcgaat ttgccctgcc
ggtcgagctg accctcgacc agcagaaggc gctggcgtcc gagttcgccc agcacctgac
cggtgccgag cgcctgccgt atacgctggc catccatgcc ggtggcggcg agaacccgca
ctgccacctg atgatctccg agcggatcaa tgacggcatc gagcggcccg ccgctcagtg
gttcaagcgg tacaacggca agaccccgga gaagggcggg gcacagaaga ccgaagcgct
caagcccaag gcatggcttg agcagacccg cgaggcatgg gccgaccatg ccaaccgggc
attagagcgg gctggccacg acgcccgcat tgaccacaga acacttgagg cgcagggcat
cgagcgcctg cccggtgttc acctggggcc gaacgtggtg gagatggaag gccggggcat
ccgcaccgac cgggcagacg tggccctgaa catcgacacc gccaacgccc agatcatcga
cttacaggaa taccgggagg caatagacca tgaacgcaat cgacagagtg aagaaatcca
gaggcatcaa cgagttagcg gagcagatcg aaccgctggc ccagagcatg gcgacactgg
ccgacgaagc ccggcaggtc atgagccaga ccaagcaggc cagcgaggcg caggcggcgg
agtggctgaa agcccagcgc cagacagggg cggcatgggt ggagctggcc aaagagttgc
gggaggtagc cgccgaggtg agcagcgccg cgcagagcgc ccggagcgcg tcgcgggggt
ggcactggaa gctatggcta accgtgatgc tggcttccat gatgcctacg gtggtgctgc
tgatcgcatc gttgctcttg ctcgacctga cgccactgac aaccgaggac ggctcgatct
ggctgcgctt ggtggcccga tgaagaacga caggactttg caggccatag gccgacagct
caaggccatg ggctgtgagc gcttcgatat cggcgtcagg gacgcaccca ccggccagat
gatgaaccgg gaatggtcag ccgccgaagt gctccagaac acgccatggc tcaagcggat
gaatgcccag ggcaatgacg tgtatatcag gcccgccgag caggagcggc atggtctggt
gctggtggac gacctcagcg agtttgacct ggatgacatg aaagccgagg gccgggagcc
tgccctggta gtggaaacca gcccgaagaa ctatcaggca tgggtcaagg tggccgacgc
cgcaggcggt gaacttcggg ggcagattgc ccggacgctg gccagcgagt acgacgccga
cccggccagc gccgacagcc gccactatgg ccgcttggcg ggcttcacca accgcaagga
caagcacacc acccgcgccg gttatcagcc gtgggtgctg ctgcgtgaat ccaagggcaa
gaccgccacc gctggcccgg cgctggtgca gcaggctggc cagcagatcg agcaggccca
gcggcagcag gagaaggccc gcaggctggc cagcctcgaa ctgcccgagc ggcagcttag
ccgccaccgg cgcacggcgc tggacgagta ccgcagcgag atggccgggc tggtcaagcg
cttcggtcat gacctcagca agtgcgactt tatcgccgcg cagaagctgg ccagccgggg
ccgcagtgcc gaggaaatcg gcaaggccat ggccgaggcc agcccagcgc tggcagagcg
caagcccggc cacgaagcgg attacatcga gcgcaccgtc agcaaggtca tgggtctgcc
cagcgtccag cttgcgcggg ccgagctggc acgggcaccg gcaccccgcc agcgaggcat
ggacaggggc gggccagatt tcagcatgta gtgcttgcgt tggtactcac gcctgttata
ctatgagtac tcacgcacag aagggggttt tatggaatac gaaaaaagcg cttcagggtc
ggtctacctg atcaaaagtg acaagggcta ttggttgccc ggtggctttg gttatacgtc
aaacaaggcc gaggctggcc gcttttcagt cgctgatatg gccagcctta accttgacgg
ctgcaccttg tccttgttcc gcgaagacaa gcctttcggc cccggcaagt ttctcggtga
ctgatatgaa agaccaaaag gacaagcaga ccggcgacct gctggccagc cctgacgctg
tacgccaagc gcgatatgcc gagcgcatga aggccaaagg gatgcgtcag cgcaagttct
ggctgaccga cgacgaatac gaggcgctgc gcgagtgcct ggaagaactc agagcggcgc
agggcggggg tagtgacccc gccagcgcct aaccaccaac tgcctgcaaa ggaggcaatc
aatggctacc cataagccta tcaatattct ggaggcgttc gcagcagcgc cgccaccgct
ggactacgtt ttgcccaaca tggtggccgg tacggtcggg gcgctggtgt cgcccggtgg
tgccggtaaa tccatgctgg ccctgcaact ggccgcacag attgcaggcg ggccggatct
gctggaggtg ggcgaactgc ccaccggccc ggtgatctac ctgcccgccg aagacccgcc
caccgccatt catcaccgcc tgcacgccct tggggcgcac ctcagcgccg aggaacggca
agccgtggct gacggcctgc tgatccagcc gctgatcggc agcctgccca acatcatggc
cccggagtgg ttcgacggcc tcaagcgcgc cgccgagggc cgccgcctga tggtgctgga
cacgctgcgc cggttccaca tcgaggaaga aaacgccagc ggccccatgg cccaggtcat
cggtcgcatg gaggccatcg ccgccgatac cgggtgctct atcgtgttcc tgcaccatgc
cagcaagggc gcggccatga tgggcgcagg cgaccagcag caggccagcc ggggcagctc
ggtactggtc gataacatcc gctggcagtc ctacctgtcg agcatgacca gcgccgaggc
cgaggaatgg ggtgtggacg acgaccagcg ccggttcttc gtccgcttcg gtgtgagcaa
ggccaactat ggcgcaccgt tcgctgatcg gtggttcagg cggcatgacg gcggggtgct
caagcccgcc gtgctggaga ggcagcgcaa gagcaagggg gtgccccgtg gtgaagccta
agaacaagca cagcctcagc cacgtccggc acgacccggc gcactgtctg gcccccggcc
tgttccgtgc cctcaagcgg ggcgagcgca agcgcagcaa gctggacgtg acgtatgact
acggcgacgg caagcggatc gagttcagcg gcccggagcc gctgggcgct gatgatctgc
gcatcctgca agggctggtg gccatggctg ggcctaatgg cctagtgctt ggcccggaac
ccaagaccga aggcggacgg cagctccggc tgttcctgga acccaagtgg gaggccgtca
ccgctgaatg ccatgtggtc aaaggtagct atcgggcgct ggcaaaggaa atcggggcag
aggtcgatag tggtggggcg ctcaagcaca tacaggactg catcgagcgc ctttggaagg
tatccatcat cgcccagaat ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg
ccagcgacga ggcggacggg cgcctgtacg tggccctgaa ccccttgatc gcgcaggccg
tcatgggtgg cggccagcat gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg
aaaccgcccg cctgctgcac cagcggctgt gtggctggat cgaccccggc aaaaccggca
aggcttccat agataccttg tgcggctatg tctggccgtc agaggccagt ggttcgacca
tgcgcaagcg ccgcaagcgg gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga
cggtaaccga gttcgcggcg ggcaagtacg acatcacccg gcccaaggcg gcaggctgac
cccccccact ctattgtaaa caagacattt ttatctttta tattcaatgg cttattttcc
tgctaattgg taataccatg aaaaatacca tgctcagaaa aggcttaaca atattttgaa
aaattgccta ctgagcgctg ccgcacagct ccataggccg ctttcctggc tttgcttcca
gatgtatgct cttctgctcc cgaacgccag caagacgtag cccagcgcgt cggccagctt
gcaattcgcg ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
accgcctggc cctgagagag ttgcagcaag cggtccacgt ggtttgcccc agcaggcgaa
aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg gtgtcaacgt
aaatgccgct tcgccttcgc gcgcgaattg caagctgatc cgggcttatc gactgcacgg
tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg
taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg
cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc
ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagaattaa
ttaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca
gcttggctgt tttggcggat gagagaaga
//