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ID PMMB67EH preliminary; circular DNA; SYN; 8828 BP.
XX
AC ATCC37622;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Broad host range/E.coli plasmid vector pMMB67EH - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pJF118EH, pJF118HE from pMMB22 & pKK223-3
RC pMMB66EH from RSF1010 & pJF118EH
RC pMMB66HE from pMMB66EH & pJF118HE
RC pJF119EH from pJF118EH & M13mp18
RC pJF119HE from pJF118HE & M13mp18
RC pMMB67EH from pMMB66EH & M13mp18
RC pMMB67HE from pMMB66HE & M13mp18
RC pMS324a from R300B & RP4
RC pFG101 series from pJF118EH & pMS324a
RC pFG102 series from pJF118HE & pMS324a
RC pWP101 series from pMMB66EH & pMS324a
RC pWP102 series from pMMB66HE & pMS324a
RA Furste J.P., Pansegrau W., Frank R., Blocker H., Scholz P.,
RA Bagdasarian M., Lanka E.;
RT "Molecular cloning of the plasmid RP4 primase region in a
RT multi-host-range tacP expression vector";
RL Gene 48:119-131(1986).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC Restriction digests of the clone give the following sizes (kb):
CC AccI--3.7, 3.1, 1.8; BamHI--8.8; HindIII/PvuII--7.2, 1.3. (ATCC
CC staff)
CC Contains the following sites separated by (bp): EcoRI - 785 - AhaIII
CC - 97 - ScaI - 112 - PvuI - 2101 - AccI - 1848 - ScaI - 55 - AccI -
CC 2039 - BstEII - 372 - PvuII - 93 - PvuII - 94 - HpaI - 320 - BstEII
CC - 182 - MluI - 732 - EcoRI. (personal communication)
CC The M13mp8 polylinker of pMMB66EH (ATCC 37620) was replaced with the
CC M13mp18 polylinker.
CC In the pMMB66 (ATCC 37620, 37621) and pMMB67 (ATCC 37622, 37623)
CC vector series,the EH and HE in the name designate the orientation of
CC the multiple cloning site to the tac promoter. (personal
CC communication)
CC Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013
CC (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis,
CC Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative
CC bacteria.
CC An autoregulated high-level expression vector utilizing the tac
CC promoter, rrnB terminators and lacIq for lac repression. Concentration
CC of induced gene product may be regulated by varying the lac inducer
CC concentration.
CC Belongs to a group of vectors (ATCC 37622-37623, 77287-77292)
CC differing only in antibiotic resistance and polylinker orientation.
CC (submitted, 1992)
CC Compatible with common E.coli cloning vectors, as well as vectors
CC derived from IncP and IncW plasmids. (submitted, 1992)
CC Medium is 1227 LB plus ampicillin.
CC NM (pMMB67EH)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli HB101)(broad host range)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pMMB66EH)(M13mp18)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pMMB66EH remove EcoRI-HindIII 30bp
FT \ 8806..8807..29, MCS/8777bp
FT 2. M13mp18 EcoRI-HindIII 51bp 6231..6282, MCS
FT -> pMMB67EH 8828bp"
FT - 1..8777
FT /note="pMMB66EH 29..8805 8777bp
FT EcoRI = G^AATTC"
FT - 8778..8828
FT /note="M13mp18 6231..6281 51bp
FT HindIII = A^AGCTT"
FT misc_binding 0..0
FT /note="MCS EcoRI-SacI-KpnI-SmaI-XmaI-BamHI-XbaI-
FT SalI-AccI-HincII-PstI-SphI-HindIII"
FT misc_binding 0..0
FT /note="SIT unique EcoRI-SacI-KpnI-SmaI-XmaI-BamHI-
FT XbaI-SalI-AccI-HincII-PstI-SphI-HindIII"
FT rep_origin 0..0
FT /note="ORI E. coli IncQ plasmid RSF1010 oriT and oriV"
FT promoter 0..0
FT /note="PRO E. coli tac"
FT CDS 0..0
FT /note="REP E. coli lacIq repressor gene"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
XX
SQ Sequence 8828 BP; 1909 A; 2581 C; 2638 G; 1700 T; 0 other;
agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac
gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg
accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc
atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
gtaactgtca gaccaagttt actcatatat actttagatt gatttctgaa agcgaccagg
tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag ccgcccgcat
tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc ctgctctgcc
ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag ggtttgctcg
ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct ggccgccaca
tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg cttagcggaa
agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt
cactcccgta ctaactgtca cgaacccctg caataactgt cacgcccccc tgcaataact
gtcacgaacc cctgcaataa ctgtcacgcc cccaaacctg caaacccagc aggggcgggg
gctggcgggg tgttggaaaa atccatccat gattatctaa gaataatcca ctaggcgcgg
ttatcagcgc ccttgtgggg cgctgctgcc cttgcccaat atgcccggcc agaggccgga
tagctggtct attcgctgcg ctaggctaca caccgcccca ccgctgcgcg gcagggggaa
aggcgggcaa agcccgctaa accccacacc aaaccccgca gaaatacgct ggagcgcttt
tagccgcttt agcggccttt ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca
gggcctgtct cggtcgatca ttcagcccgg ctcatccttc tggcgtggcg gcagaccgaa
caaggcgcgg tcgtggtcgc gttcaaggta cgcatccatt gccgccatga gccgatcctc
cggccactcg ctgctgttca ccttggccaa aatcatggcc cccaccagca ccttgcgcct
tgtttcgttc ttgcgctctt gctgctgttc ccttgcccgc tcccgctgaa tttcggcatt
gattcgcgct cgttgttctt cgagcttggc cagccgatcc gccgccttgt tgctcccctt
aaccatcttg acaccccatt gttaatgtgc tgtctcgtag gctatcatgg aggcacagcg
gcggcaatcc cgaccctact ttgtagggga gggcgcactt accggtttct cttcgagaaa
ctggcctaac ggccaccctt cgggcggtgc gctctccgag ggccattgca tggagccgaa
aagcaaaagc aacagcgagg cagcatggcg atttatcacc ttacggcgaa aaccggcagc
aggtcgggcg gccaatcggc cagggccaag gccgactaca tccagcgcga aggcaagtat
gcccgcgaca tggatgaagt cttgcacgcc gaatccgggc acatgccgga gttcgtcgag
cggcccgccg actactggga tgctgccgac ctgtatgaac gcgccaatgg gcggctgttc
aaggaggtcg aatttgccct gccggtcgag ctgaccctcg accagcagaa ggcgctggcg
tccgagttcg cccagcacct gaccggtgcc gagcgcctgc cgtatacgct ggccatccat
gccggtggcg gcgagaaccc gcactgccac ctgatgatct ccgagcggat caatgacggc
atcgagcggc ccgccgctca gtggttcaag cggtacaacg gcaagacccc ggagaagggc
ggggcacaga agaccgaagc gctcaagccc aaggcatggc ttgagcagac ccgcgaggca
tgggccgacc atgccaaccg ggcattagag cgggctggcc acgacgcccg cattgaccac
agaacacttg aggcgcaggg catcgagcgc ctgcccggtg ttcacctggg gccgaacgtg
gtggagatgg aaggccgggg catccgcacc gaccgggcag acgtggccct gaacatcgac
accgccaacg cccagatcat cgacttacag gaataccggg aggcaataga ccatgaacgc
aatcgacaga gtgaagaaat ccagaggcat caacgagtta gcggagcaga tcgaaccgct
ggcccagagc atggcgacac tggccgacga agcccggcag gtcatgagcc agaccaagca
ggccagcgag gcgcaggcgg cggagtggct gaaagcccag cgccagacag gggcggcatg
ggtggagctg gccaaagagt tgcgggaggt agccgccgag gtgagcagcg ccgcgcagag
cgcccggagc gcgtcgcggg ggtggcactg gaagctatgg ctaaccgtga tgctggcttc
catgatgcct acggtggtgc tgctgatcgc atcgttgctc ttgctcgacc tgacgccact
gacaaccgag gacggctcga tctggctgcg cttggtggcc cgatgaagaa cgacaggact
ttgcaggcca taggccgaca gctcaaggcc atgggctgtg agcgcttcga tatcggcgtc
agggacgcac ccaccggcca gatgatgaac cgggaatggt cagccgccga agtgctccag
aacacgccat ggctcaagcg gatgaatgcc cagggcaatg acgtgtatat caggcccgcc
gagcaggagc ggcatggtct ggtgctggtg gacgacctca gcgagtttga cctggatgac
atgaaagccg agggccggga gcctgccctg gtagtggaaa ccagcccgaa gaactatcag
gcatgggtca aggtggccga cgccgcaggc ggtgaacttc gggggcagat tgcccggacg
ctggccagcg agtacgacgc cgacccggcc agcgccgaca gccgccacta tggccgcttg
gcgggcttca ccaaccgcaa ggacaagcac accacccgcg ccggttatca gccgtgggtg
ctgctgcgtg aatccaaggg caagaccgcc accgctggcc cggcgctggt gcagcaggct
ggccagcaga tcgagcaggc ccagcggcag caggagaagg cccgcaggct ggccagcctc
gaactgcccg agcggcagct tagccgccac cggcgcacgg cgctggacga gtaccgcagc
gagatggccg ggctggtcaa gcgcttcggt catgacctca gcaagtgcga ctttatcgcc
gcgcagaagc tggccagccg gggccgcagt gccgaggaaa tcggcaaggc catggccgag
gccagcccag cgctggcaga gcgcaagccc ggccacgaag cggattacat cgagcgcacc
gtcagcaagg tcatgggtct gcccagcgtc cagcttgcgc gggccgagct ggcacgggca
ccggcacccc gccagcgagg catggacagg ggcgggccag atttcagcat gtagtgcttg
cgttggtact cacgcctgtt atactatgag tactcacgca cagaaggggg ttttatggaa
tacgaaaaaa gcgcttcagg gtcggtctac ctgatcaaaa gtgacaaggg ctattggttg
cccggtggct ttggttatac gtcaaacaag gccgaggctg gccgcttttc agtcgctgat
atggccagcc ttaaccttga cggctgcacc ttgtccttgt tccgcgaaga caagcctttc
ggccccggca agtttctcgg tgactgatat gaaagaccaa aaggacaagc agaccggcga
cctgctggcc agccctgacg ctgtacgcca agcgcgatat gccgagcgca tgaaggccaa
agggatgcgt cagcgcaagt tctggctgac cgacgacgaa tacgaggcgc tgcgcgagtg
cctggaagaa ctcagagcgg cgcagggcgg gggtagtgac cccgccagcg cctaaccacc
aactgcctgc aaaggaggca atcaatggct acccataagc ctatcaatat tctggaggcg
ttcgcagcag cgccgccacc gctggactac gttttgccca acatggtggc cggtacggtc
ggggcgctgg tgtcgcccgg tggtgccggt aaatccatgc tggccctgca actggccgca
cagattgcag gcgggccgga tctgctggag gtgggcgaac tgcccaccgg cccggtgatc
tacctgcccg ccgaagaccc gcccaccgcc attcatcacc gcctgcacgc ccttggggcg
cacctcagcg ccgaggaacg gcaagccgtg gctgacggcc tgctgatcca gccgctgatc
ggcagcctgc ccaacatcat ggccccggag tggttcgacg gcctcaagcg cgccgccgag
ggccgccgcc tgatggtgct ggacacgctg cgccggttcc acatcgagga agaaaacgcc
agcggcccca tggcccaggt catcggtcgc atggaggcca tcgccgccga taccgggtgc
tctatcgtgt tcctgcacca tgccagcaag ggcgcggcca tgatgggcgc aggcgaccag
cagcaggcca gccggggcag ctcggtactg gtcgataaca tccgctggca gtcctacctg
tcgagcatga ccagcgccga ggccgaggaa tggggtgtgg acgacgacca gcgccggttc
ttcgtccgct tcggtgtgag caaggccaac tatggcgcac cgttcgctga tcggtggttc
aggcggcatg acggcggggt gctcaagccc gccgtgctgg agaggcagcg caagagcaag
ggggtgcccc gtggtgaagc ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc
ggcgcactgt ctggcccccg gcctgttccg tgccctcaag cggggcgagc gcaagcgcag
caagctggac gtgacgtatg actacggcga cggcaagcgg atcgagttca gcggcccgga
gccgctgggc gctgatgatc tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa
tggcctagtg cttggcccgg aacccaagac cgaaggcgga cggcagctcc ggctgttcct
ggaacccaag tgggaggccg tcaccgctga atgccatgtg gtcaaaggta gctatcgggc
gctggcaaag gaaatcgggg cagaggtcga tagtggtggg gcgctcaagc acatacagga
ctgcatcgag cgcctttgga aggtatccat catcgcccag aatggccgca agcggcaggg
gtttcggctg ctgtcggagt acgccagcga cgaggcggac gggcgcctgt acgtggccct
gaaccccttg atcgcgcagg ccgtcatggg tggcggccag catgtgcgca tcagcatgga
cgaggtgcgg gcgctggaca gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg
gatcgacccc ggcaaaaccg gcaaggcttc catagatacc ttgtgcggct atgtctggcc
gtcagaggcc agtggttcga ccatgcgcaa gcgccgcaag cgggtgcgcg aggcgttgcc
ggagctggtc gcgctgggct ggacggtaac cgagttcgcg gcgggcaagt acgacatcac
ccggcccaag gcggcaggct gacccccccc actctattgt aaacaagaca tttttatctt
ttatattcaa tggcttattt tcctgctaat tggtaatacc atgaaaaata ccatgctcag
aaaaggctta acaatatttt gaaaaattgc ctactgagcg ctgccgcaca gctccatagg
ccgctttcct ggctttgctt ccagatgtat gctcttctgc tcccgaacgc cagcaagacg
tagcccagcg cgtcggccag cttgcaattc gcgctaactt acattaattg cgttgcgctc
actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
cgtggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca
tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc
ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc
agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact
ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca
gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg
ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa
aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt
gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc
actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg
ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc
gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga
ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc
cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga
aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac
attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
gcaccattcg atggtgtcaa cgtaaatgcc gcttcgcctt cgcgcgcgaa ttgcaagctg
atccgggctt atcgactgca cggtgcacca atgcttctgg cgtcaggcag ccatcggaag
ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact
cccgttctgg ataatgtttt ttgcgccgac atcataacgg ttctggcaaa tattctgaaa
tgagctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag cggataacaa
tttcacacag gaaacagaat tcgagctcgg tacccgggga tcctctagag tcgacctgca
ggcatgca
//