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ID   PMMB67EH   preliminary; circular DNA; SYN; 8828 BP.
XX
AC   ATCC37622;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Broad host range/E.coli plasmid vector pMMB67EH - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pJF118EH, pJF118HE from pMMB22 & pKK223-3
RC   pMMB66EH from RSF1010 & pJF118EH
RC   pMMB66HE from pMMB66EH & pJF118HE
RC   pJF119EH from pJF118EH & M13mp18
RC   pJF119HE from pJF118HE & M13mp18
RC   pMMB67EH from pMMB66EH & M13mp18
RC   pMMB67HE from pMMB66HE & M13mp18
RC   pMS324a from R300B & RP4
RC   pFG101 series from pJF118EH & pMS324a
RC   pFG102 series from pJF118HE & pMS324a
RC   pWP101 series from pMMB66EH & pMS324a
RC   pWP102 series from pMMB66HE & pMS324a
RA   Furste J.P., Pansegrau W., Frank R., Blocker H., Scholz P.,
RA   Bagdasarian M., Lanka E.;
RT   "Molecular cloning of the plasmid RP4 primase region in a
RT   multi-host-range tacP expression vector";
RL   Gene 48:119-131(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Restriction digests of the clone give the following sizes (kb):
CC   AccI--3.7, 3.1, 1.8; BamHI--8.8; HindIII/PvuII--7.2, 1.3. (ATCC
CC   staff)
CC   Contains the following sites separated by (bp): EcoRI - 785 - AhaIII
CC   - 97 - ScaI - 112 - PvuI - 2101 - AccI - 1848 - ScaI - 55 - AccI -
CC   2039 - BstEII - 372 - PvuII - 93 - PvuII - 94 - HpaI - 320 - BstEII
CC   - 182 - MluI - 732 - EcoRI. (personal communication)
CC   The M13mp8 polylinker of pMMB66EH (ATCC 37620) was replaced with the
CC   M13mp18 polylinker.
CC   In the pMMB66 (ATCC 37620, 37621) and pMMB67 (ATCC 37622, 37623)
CC   vector series,the EH and HE in the name designate the orientation of
CC   the multiple cloning site to the tac promoter. (personal
CC   communication)
CC   Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013
CC   (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis,
CC   Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative
CC   bacteria.
CC   An autoregulated high-level expression vector utilizing the tac
CC   promoter, rrnB terminators and lacIq for lac repression. Concentration
CC   of induced gene product may be regulated by varying the lac inducer
CC   concentration.
CC   Belongs to a group of vectors (ATCC 37622-37623, 77287-77292)
CC   differing only in antibiotic resistance and polylinker orientation.
CC   (submitted, 1992)
CC   Compatible with common E.coli cloning vectors, as well as vectors
CC   derived from IncP and IncW plasmids. (submitted, 1992)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pMMB67EH)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pMMB66EH)(M13mp18)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMMB66EH remove EcoRI-HindIII 30bp
FT                   \ 8806..8807..29, MCS/8777bp
FT                   2. M13mp18 EcoRI-HindIII 51bp 6231..6282, MCS
FT                   -> pMMB67EH 8828bp"
FT   -               1..8777
FT                   /note="pMMB66EH 29..8805 8777bp
FT                   EcoRI = G^AATTC"
FT   -               8778..8828
FT                   /note="M13mp18 6231..6281 51bp
FT                   HindIII = A^AGCTT"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-XmaI-BamHI-XbaI-
FT                   SalI-AccI-HincII-PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SacI-KpnI-SmaI-XmaI-BamHI-
FT                   XbaI-SalI-AccI-HincII-PstI-SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli IncQ plasmid RSF1010 oriT and oriV"
FT   promoter        0..0
FT                   /note="PRO E. coli tac"
FT   CDS             0..0
FT                   /note="REP E. coli lacIq repressor gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 8828 BP; 1909 A; 2581 C; 2638 G; 1700 T; 0 other;
     agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac
     gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg
     accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc
     atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
     taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
     ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     gtaactgtca gaccaagttt actcatatat actttagatt gatttctgaa agcgaccagg
     tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag ccgcccgcat
     tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc ctgctctgcc
     ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag ggtttgctcg
     ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct ggccgccaca
     tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg cttagcggaa
     agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt
     cactcccgta ctaactgtca cgaacccctg caataactgt cacgcccccc tgcaataact
     gtcacgaacc cctgcaataa ctgtcacgcc cccaaacctg caaacccagc aggggcgggg
     gctggcgggg tgttggaaaa atccatccat gattatctaa gaataatcca ctaggcgcgg
     ttatcagcgc ccttgtgggg cgctgctgcc cttgcccaat atgcccggcc agaggccgga
     tagctggtct attcgctgcg ctaggctaca caccgcccca ccgctgcgcg gcagggggaa
     aggcgggcaa agcccgctaa accccacacc aaaccccgca gaaatacgct ggagcgcttt
     tagccgcttt agcggccttt ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca
     gggcctgtct cggtcgatca ttcagcccgg ctcatccttc tggcgtggcg gcagaccgaa
     caaggcgcgg tcgtggtcgc gttcaaggta cgcatccatt gccgccatga gccgatcctc
     cggccactcg ctgctgttca ccttggccaa aatcatggcc cccaccagca ccttgcgcct
     tgtttcgttc ttgcgctctt gctgctgttc ccttgcccgc tcccgctgaa tttcggcatt
     gattcgcgct cgttgttctt cgagcttggc cagccgatcc gccgccttgt tgctcccctt
     aaccatcttg acaccccatt gttaatgtgc tgtctcgtag gctatcatgg aggcacagcg
     gcggcaatcc cgaccctact ttgtagggga gggcgcactt accggtttct cttcgagaaa
     ctggcctaac ggccaccctt cgggcggtgc gctctccgag ggccattgca tggagccgaa
     aagcaaaagc aacagcgagg cagcatggcg atttatcacc ttacggcgaa aaccggcagc
     aggtcgggcg gccaatcggc cagggccaag gccgactaca tccagcgcga aggcaagtat
     gcccgcgaca tggatgaagt cttgcacgcc gaatccgggc acatgccgga gttcgtcgag
     cggcccgccg actactggga tgctgccgac ctgtatgaac gcgccaatgg gcggctgttc
     aaggaggtcg aatttgccct gccggtcgag ctgaccctcg accagcagaa ggcgctggcg
     tccgagttcg cccagcacct gaccggtgcc gagcgcctgc cgtatacgct ggccatccat
     gccggtggcg gcgagaaccc gcactgccac ctgatgatct ccgagcggat caatgacggc
     atcgagcggc ccgccgctca gtggttcaag cggtacaacg gcaagacccc ggagaagggc
     ggggcacaga agaccgaagc gctcaagccc aaggcatggc ttgagcagac ccgcgaggca
     tgggccgacc atgccaaccg ggcattagag cgggctggcc acgacgcccg cattgaccac
     agaacacttg aggcgcaggg catcgagcgc ctgcccggtg ttcacctggg gccgaacgtg
     gtggagatgg aaggccgggg catccgcacc gaccgggcag acgtggccct gaacatcgac
     accgccaacg cccagatcat cgacttacag gaataccggg aggcaataga ccatgaacgc
     aatcgacaga gtgaagaaat ccagaggcat caacgagtta gcggagcaga tcgaaccgct
     ggcccagagc atggcgacac tggccgacga agcccggcag gtcatgagcc agaccaagca
     ggccagcgag gcgcaggcgg cggagtggct gaaagcccag cgccagacag gggcggcatg
     ggtggagctg gccaaagagt tgcgggaggt agccgccgag gtgagcagcg ccgcgcagag
     cgcccggagc gcgtcgcggg ggtggcactg gaagctatgg ctaaccgtga tgctggcttc
     catgatgcct acggtggtgc tgctgatcgc atcgttgctc ttgctcgacc tgacgccact
     gacaaccgag gacggctcga tctggctgcg cttggtggcc cgatgaagaa cgacaggact
     ttgcaggcca taggccgaca gctcaaggcc atgggctgtg agcgcttcga tatcggcgtc
     agggacgcac ccaccggcca gatgatgaac cgggaatggt cagccgccga agtgctccag
     aacacgccat ggctcaagcg gatgaatgcc cagggcaatg acgtgtatat caggcccgcc
     gagcaggagc ggcatggtct ggtgctggtg gacgacctca gcgagtttga cctggatgac
     atgaaagccg agggccggga gcctgccctg gtagtggaaa ccagcccgaa gaactatcag
     gcatgggtca aggtggccga cgccgcaggc ggtgaacttc gggggcagat tgcccggacg
     ctggccagcg agtacgacgc cgacccggcc agcgccgaca gccgccacta tggccgcttg
     gcgggcttca ccaaccgcaa ggacaagcac accacccgcg ccggttatca gccgtgggtg
     ctgctgcgtg aatccaaggg caagaccgcc accgctggcc cggcgctggt gcagcaggct
     ggccagcaga tcgagcaggc ccagcggcag caggagaagg cccgcaggct ggccagcctc
     gaactgcccg agcggcagct tagccgccac cggcgcacgg cgctggacga gtaccgcagc
     gagatggccg ggctggtcaa gcgcttcggt catgacctca gcaagtgcga ctttatcgcc
     gcgcagaagc tggccagccg gggccgcagt gccgaggaaa tcggcaaggc catggccgag
     gccagcccag cgctggcaga gcgcaagccc ggccacgaag cggattacat cgagcgcacc
     gtcagcaagg tcatgggtct gcccagcgtc cagcttgcgc gggccgagct ggcacgggca
     ccggcacccc gccagcgagg catggacagg ggcgggccag atttcagcat gtagtgcttg
     cgttggtact cacgcctgtt atactatgag tactcacgca cagaaggggg ttttatggaa
     tacgaaaaaa gcgcttcagg gtcggtctac ctgatcaaaa gtgacaaggg ctattggttg
     cccggtggct ttggttatac gtcaaacaag gccgaggctg gccgcttttc agtcgctgat
     atggccagcc ttaaccttga cggctgcacc ttgtccttgt tccgcgaaga caagcctttc
     ggccccggca agtttctcgg tgactgatat gaaagaccaa aaggacaagc agaccggcga
     cctgctggcc agccctgacg ctgtacgcca agcgcgatat gccgagcgca tgaaggccaa
     agggatgcgt cagcgcaagt tctggctgac cgacgacgaa tacgaggcgc tgcgcgagtg
     cctggaagaa ctcagagcgg cgcagggcgg gggtagtgac cccgccagcg cctaaccacc
     aactgcctgc aaaggaggca atcaatggct acccataagc ctatcaatat tctggaggcg
     ttcgcagcag cgccgccacc gctggactac gttttgccca acatggtggc cggtacggtc
     ggggcgctgg tgtcgcccgg tggtgccggt aaatccatgc tggccctgca actggccgca
     cagattgcag gcgggccgga tctgctggag gtgggcgaac tgcccaccgg cccggtgatc
     tacctgcccg ccgaagaccc gcccaccgcc attcatcacc gcctgcacgc ccttggggcg
     cacctcagcg ccgaggaacg gcaagccgtg gctgacggcc tgctgatcca gccgctgatc
     ggcagcctgc ccaacatcat ggccccggag tggttcgacg gcctcaagcg cgccgccgag
     ggccgccgcc tgatggtgct ggacacgctg cgccggttcc acatcgagga agaaaacgcc
     agcggcccca tggcccaggt catcggtcgc atggaggcca tcgccgccga taccgggtgc
     tctatcgtgt tcctgcacca tgccagcaag ggcgcggcca tgatgggcgc aggcgaccag
     cagcaggcca gccggggcag ctcggtactg gtcgataaca tccgctggca gtcctacctg
     tcgagcatga ccagcgccga ggccgaggaa tggggtgtgg acgacgacca gcgccggttc
     ttcgtccgct tcggtgtgag caaggccaac tatggcgcac cgttcgctga tcggtggttc
     aggcggcatg acggcggggt gctcaagccc gccgtgctgg agaggcagcg caagagcaag
     ggggtgcccc gtggtgaagc ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc
     ggcgcactgt ctggcccccg gcctgttccg tgccctcaag cggggcgagc gcaagcgcag
     caagctggac gtgacgtatg actacggcga cggcaagcgg atcgagttca gcggcccgga
     gccgctgggc gctgatgatc tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa
     tggcctagtg cttggcccgg aacccaagac cgaaggcgga cggcagctcc ggctgttcct
     ggaacccaag tgggaggccg tcaccgctga atgccatgtg gtcaaaggta gctatcgggc
     gctggcaaag gaaatcgggg cagaggtcga tagtggtggg gcgctcaagc acatacagga
     ctgcatcgag cgcctttgga aggtatccat catcgcccag aatggccgca agcggcaggg
     gtttcggctg ctgtcggagt acgccagcga cgaggcggac gggcgcctgt acgtggccct
     gaaccccttg atcgcgcagg ccgtcatggg tggcggccag catgtgcgca tcagcatgga
     cgaggtgcgg gcgctggaca gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg
     gatcgacccc ggcaaaaccg gcaaggcttc catagatacc ttgtgcggct atgtctggcc
     gtcagaggcc agtggttcga ccatgcgcaa gcgccgcaag cgggtgcgcg aggcgttgcc
     ggagctggtc gcgctgggct ggacggtaac cgagttcgcg gcgggcaagt acgacatcac
     ccggcccaag gcggcaggct gacccccccc actctattgt aaacaagaca tttttatctt
     ttatattcaa tggcttattt tcctgctaat tggtaatacc atgaaaaata ccatgctcag
     aaaaggctta acaatatttt gaaaaattgc ctactgagcg ctgccgcaca gctccatagg
     ccgctttcct ggctttgctt ccagatgtat gctcttctgc tcccgaacgc cagcaagacg
     tagcccagcg cgtcggccag cttgcaattc gcgctaactt acattaattg cgttgcgctc
     actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     cgtggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca
     tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc
     ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc
     agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact
     ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca
     gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg
     ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa
     aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt
     gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc
     actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg
     ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc
     gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga
     ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc
     cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga
     aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac
     attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
     gcaccattcg atggtgtcaa cgtaaatgcc gcttcgcctt cgcgcgcgaa ttgcaagctg
     atccgggctt atcgactgca cggtgcacca atgcttctgg cgtcaggcag ccatcggaag
     ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact
     cccgttctgg ataatgtttt ttgcgccgac atcataacgg ttctggcaaa tattctgaaa
     tgagctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag cggataacaa
     tttcacacag gaaacagaat tcgagctcgg tacccgggga tcctctagag tcgacctgca
     ggcatgca
//