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ID   PMMB67HE   preliminary; circular DNA; SYN; 8842 BP.
XX
AC   ATCC37623;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Broad host range/E.coli plasmid vector pMMB67HE - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pJF118EH, pJF118HE from pMMB22 & pKK223-3
RC   pMMB66EH from RSF1010 & pJF118EH
RC   pMMB66HE from pMMB66EH & pJF118HE
RC   pJF119EH from pJF118EH & M13mp18
RC   pJF119HE from pJF118HE & M13mp18
RC   pMMB67EH from pMMB66EH & M13mp18
RC   pMMB67HE from pMMB66HE & M13mp18
RC   pMS324a from R300B & RP4
RC   pFG101 series from pJF118EH & pMS324a
RC   pFG102 series from pJF118HE & pMS324a
RC   pWP101 series from pMMB66EH & pMS324a
RC   pWP102 series from pMMB66HE & pMS324a
RA   Furste J.P., Pansegrau W., Frank R., Blocker H., Scholz P.,
RA   Bagdasarian M., Lanka E.;
RT   "Molecular cloning of the plasmid RP4 primase region in a
RT   multi-host-range tacP expression vector";
RL   Gene 48:119-131(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Restriction digests of the clone give the following sizes (kb):
CC   AccI--3.9, 3.0, 1.9; BamHI--8.8; HindIII/PvuII--7.6, 1.1. (ATCC
CC   staff)
CC   Contains the following sites separated by (bp): EcoRI - 739 - AhaIII
CC   - 97 - ScaI - 112 - PvuI - 2101 - AccI - 1848 - ScaI - 55 - AccI -
CC   2039 - BstEII - 372 - PvuII - 93 - PvuII - 94 - HpaI - 320 - BstEII
CC   - 182 - MluI - 788 - EcoRI. (personal communication)
CC   The M13mp9 polylinker of pMMB66HE (ATCC 37621) was replaced with the
CC   M13mp19 polylinker.
CC   In the pMMB66 (ATCC 37620, 37621) and pMMB67 (ATCC 37622, 37623)
CC   vector series,the EH and HE in the name designate the orientation of
CC   the multiple cloning site to the tac promoter. (personal
CC   communication)
CC   An autoregulated high-level expression vector utilizing the tac
CC   promoter, rrnB terminators and lacIq for lac repression. Concentration
CC   of induced gene product may be regulated by varying the lac inducer
CC   concentration.
CC   Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013
CC   (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis,
CC   Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative
CC   bacteria.
CC   Belongs to a group of vectors (ATCC 37622-37623, 77287-77292)
CC   differing only in antibiotic resistance and polylinker orientation.
CC   (submitted, 1992)
CC   Compatible with common E.coli cloning vectors, as well as vectors
CC   derived from IncP and IncW plasmids. (submitted, 1992)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pMMB67HE)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMMB66HE remove HindIII-EcoRI 30bp
FT                   \ 6..36, MCS/8791bp
FT                   2. M13mp19 HindIII-EcoRI 51bp 6235..6286, MCS
FT                   -> pMMB67HE 8840bp"
FT   -               1..5
FT                   /note="pMMB66HE 1..5 5bp
FT                   HindIII = A^AGCTT"
FT   -               6..56
FT                   /note="M13mp19 6235..6285 51bp
FT                   EcoRI = G^AATTC"
FT   -               57..8842
FT                   /note="pMMB66HE 36..8821 8786bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-SalI-AccI-HincII-XbaI-
FT                   BamHI-SmaI-XmaI-KpnI-SacI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-SphI-PstI-SalI-AccI-
FT                   HincII-XbaI-BamHI-SmaI-XmaI-KpnI-SacI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli IncQ plasmid RSF1010 oriT and oriV"
FT   promoter        0..0
FT                   /note="PRO E. coli tac"
FT   CDS             0..0
FT                   /note="REP E. coli lacIq repressor gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 8842 BP; 1914 A; 2583 C; 2639 G; 1706 T; 0 other;
     aattaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt
     cagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa
     cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct
     gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc
     catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
     ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
     ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt
     tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     ggtaactgtc agaccaagtt tactcatata tactttagat tgatttctga aagcgaccag
     gtgctcggcg tggcaagact cgcagcgaac ccgtagaaag ccatgctcca gccgcccgca
     ttggagaaat tcttcaaatt cccgttgcac atagcccggc aattcctttc cctgctctgc
     cataagcgca gcgaatgccg ggtaatactc gtcaacgatc tgatagagaa gggtttgctc
     gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg ctggccgtcc tggccgccac
     atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat acagtctatc gcttagcgga
     aagttctttt accctcagcc gaaatgcctg ccgttgctag acattgccag ccagtgcccg
     tcactcccgt actaactgtc acgaacccct gcaataactg tcacgccccc ctgcaataac
     tgtcacgaac ccctgcaata actgtcacgc ccccaaacct gcaaacccag caggggcggg
     ggctggcggg gtgttggaaa aatccatcca tgattatcta agaataatcc actaggcgcg
     gttatcagcg cccttgtggg gcgctgctgc ccttgcccaa tatgcccggc cagaggccgg
     atagctggtc tattcgctgc gctaggctac acaccgcccc accgctgcgc ggcaggggga
     aaggcgggca aagcccgcta aaccccacac caaaccccgc agaaatacgc tggagcgctt
     ttagccgctt tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt gcagccccgc
     agggcctgtc tcggtcgatc attcagcccg gctcatcctt ctggcgtggc ggcagaccga
     acaaggcgcg gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg agccgatcct
     ccggccactc gctgctgttc accttggcca aaatcatggc ccccaccagc accttgcgcc
     ttgtttcgtt cttgcgctct tgctgctgtt cccttgcccg ctcccgctga atttcggcat
     tgattcgcgc tcgttgttct tcgagcttgg ccagccgatc cgccgccttg ttgctcccct
     taaccatctt gacaccccat tgttaatgtg ctgtctcgta ggctatcatg gaggcacagc
     ggcggcaatc ccgaccctac tttgtagggg agggcgcact taccggtttc tcttcgagaa
     actggcctaa cggccaccct tcgggcggtg cgctctccga gggccattgc atggagccga
     aaagcaaaag caacagcgag gcagcatggc gatttatcac cttacggcga aaaccggcag
     caggtcgggc ggccaatcgg ccagggccaa ggccgactac atccagcgcg aaggcaagta
     tgcccgcgac atggatgaag tcttgcacgc cgaatccggg cacatgccgg agttcgtcga
     gcggcccgcc gactactggg atgctgccga cctgtatgaa cgcgccaatg ggcggctgtt
     caaggaggtc gaatttgccc tgccggtcga gctgaccctc gaccagcaga aggcgctggc
     gtccgagttc gcccagcacc tgaccggtgc cgagcgcctg ccgtatacgc tggccatcca
     tgccggtggc ggcgagaacc cgcactgcca cctgatgatc tccgagcgga tcaatgacgg
     catcgagcgg cccgccgctc agtggttcaa gcggtacaac ggcaagaccc cggagaaggg
     cggggcacag aagaccgaag cgctcaagcc caaggcatgg cttgagcaga cccgcgaggc
     atgggccgac catgccaacc gggcattaga gcgggctggc cacgacgccc gcattgacca
     cagaacactt gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg ggccgaacgt
     ggtggagatg gaaggccggg gcatccgcac cgaccgggca gacgtggccc tgaacatcga
     caccgccaac gcccagatca tcgacttaca ggaataccgg gaggcaatag accatgaacg
     caatcgacag agtgaagaaa tccagaggca tcaacgagtt agcggagcag atcgaaccgc
     tggcccagag catggcgaca ctggccgacg aagcccggca ggtcatgagc cagaccaagc
     aggccagcga ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca ggggcggcat
     gggtggagct ggccaaagag ttgcgggagg tagccgccga ggtgagcagc gccgcgcaga
     gcgcccggag cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg atgctggctt
     ccatgatgcc tacggtggtg ctgctgatcg catcgttgct cttgctcgac ctgacgccac
     tgacaaccga ggacggctcg atctggctgc gcttggtggc ccgatgaaga acgacaggac
     tttgcaggcc ataggccgac agctcaaggc catgggctgt gagcgcttcg atatcggcgt
     cagggacgca cccaccggcc agatgatgaa ccgggaatgg tcagccgccg aagtgctcca
     gaacacgcca tggctcaagc ggatgaatgc ccagggcaat gacgtgtata tcaggcccgc
     cgagcaggag cggcatggtc tggtgctggt ggacgacctc agcgagtttg acctggatga
     catgaaagcc gagggccggg agcctgccct ggtagtggaa accagcccga agaactatca
     ggcatgggtc aaggtggccg acgccgcagg cggtgaactt cgggggcaga ttgcccggac
     gctggccagc gagtacgacg ccgacccggc cagcgccgac agccgccact atggccgctt
     ggcgggcttc accaaccgca aggacaagca caccacccgc gccggttatc agccgtgggt
     gctgctgcgt gaatccaagg gcaagaccgc caccgctggc ccggcgctgg tgcagcaggc
     tggccagcag atcgagcagg cccagcggca gcaggagaag gcccgcaggc tggccagcct
     cgaactgccc gagcggcagc ttagccgcca ccggcgcacg gcgctggacg agtaccgcag
     cgagatggcc gggctggtca agcgcttcgg tcatgacctc agcaagtgcg actttatcgc
     cgcgcagaag ctggccagcc ggggccgcag tgccgaggaa atcggcaagg ccatggccga
     ggccagccca gcgctggcag agcgcaagcc cggccacgaa gcggattaca tcgagcgcac
     cgtcagcaag gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc tggcacgggc
     accggcaccc cgccagcgag gcatggacag gggcgggcca gatttcagca tgtagtgctt
     gcgttggtac tcacgcctgt tatactatga gtactcacgc acagaagggg gttttatgga
     atacgaaaaa agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg gctattggtt
     gcccggtggc tttggttata cgtcaaacaa ggccgaggct ggccgctttt cagtcgctga
     tatggccagc cttaaccttg acggctgcac cttgtccttg ttccgcgaag acaagccttt
     cggccccggc aagtttctcg gtgactgata tgaaagacca aaaggacaag cagaccggcg
     acctgctggc cagccctgac gctgtacgcc aagcgcgata tgccgagcgc atgaaggcca
     aagggatgcg tcagcgcaag ttctggctga ccgacgacga atacgaggcg ctgcgcgagt
     gcctggaaga actcagagcg gcgcagggcg ggggtagtga ccccgccagc gcctaaccac
     caactgcctg caaaggaggc aatcaatggc tacccataag cctatcaata ttctggaggc
     gttcgcagca gcgccgccac cgctggacta cgttttgccc aacatggtgg ccggtacggt
     cggggcgctg gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc aactggccgc
     acagattgca ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg gcccggtgat
     ctacctgccc gccgaagacc cgcccaccgc cattcatcac cgcctgcacg cccttggggc
     gcacctcagc gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc agccgctgat
     cggcagcctg cccaacatca tggccccgga gtggttcgac ggcctcaagc gcgccgccga
     gggccgccgc ctgatggtgc tggacacgct gcgccggttc cacatcgagg aagaaaacgc
     cagcggcccc atggcccagg tcatcggtcg catggaggcc atcgccgccg ataccgggtg
     ctctatcgtg ttcctgcacc atgccagcaa gggcgcggcc atgatgggcg caggcgacca
     gcagcaggcc agccggggca gctcggtact ggtcgataac atccgctggc agtcctacct
     gtcgagcatg accagcgccg aggccgagga atggggtgtg gacgacgacc agcgccggtt
     cttcgtccgc ttcggtgtga gcaaggccaa ctatggcgca ccgttcgctg atcggtggtt
     caggcggcat gacggcgggg tgctcaagcc cgccgtgctg gagaggcagc gcaagagcaa
     gggggtgccc cgtggtgaag cctaagaaca agcacagcct cagccacgtc cggcacgacc
     cggcgcactg tctggccccc ggcctgttcc gtgccctcaa gcggggcgag cgcaagcgca
     gcaagctgga cgtgacgtat gactacggcg acggcaagcg gatcgagttc agcggcccgg
     agccgctggg cgctgatgat ctgcgcatcc tgcaagggct ggtggccatg gctgggccta
     atggcctagt gcttggcccg gaacccaaga ccgaaggcgg acggcagctc cggctgttcc
     tggaacccaa gtgggaggcc gtcaccgctg aatgccatgt ggtcaaaggt agctatcggg
     cgctggcaaa ggaaatcggg gcagaggtcg atagtggtgg ggcgctcaag cacatacagg
     actgcatcga gcgcctttgg aaggtatcca tcatcgccca gaatggccgc aagcggcagg
     ggtttcggct gctgtcggag tacgccagcg acgaggcgga cgggcgcctg tacgtggccc
     tgaacccctt gatcgcgcag gccgtcatgg gtggcggcca gcatgtgcgc atcagcatgg
     acgaggtgcg ggcgctggac agcgaaaccg cccgcctgct gcaccagcgg ctgtgtggct
     ggatcgaccc cggcaaaacc ggcaaggctt ccatagatac cttgtgcggc tatgtctggc
     cgtcagaggc cagtggttcg accatgcgca agcgccgcaa gcgggtgcgc gaggcgttgc
     cggagctggt cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag tacgacatca
     cccggcccaa ggcggcaggc tgaccccccc cactctattg taaacaagac atttttatct
     tttatattca atggcttatt ttcctgctaa ttggtaatac catgaaaaat accatgctca
     gaaaaggctt aacaatattt tgaaaaattg cctactgagc gctgccgcac agctccatag
     gccgctttcc tggctttgct tccagatgta tgctcttctg ctcccgaacg ccagcaagac
     gtagcccagc gcgtcggcca gcttgcaatt cgcgctaact tacattaatt gcgttgcgct
     cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag
     acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc
     acgtggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
     atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc
     cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
     tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
     agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
     aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
     tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
     cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
     gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
     cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
     actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
     ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
     aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
     cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
     tgcaccattc gatggtgtca acgtaaatgc cgcttcgcct tcgcgcgcga attgcaagct
     gatccgggct tatcgactgc acggtgcacc aatgcttctg gcgtcaggca gccatcggaa
     gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac
     tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa atattctgaa
     atgagctgtt gacaattaat catcggctcg tataatgtgt ggaattgtga gcggataaca
     atttcacaca ggaaacagaa tt
//