Return to this vector's summary.
ID PMMB67HE preliminary; circular DNA; SYN; 8842 BP.
XX
AC ATCC37623;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Broad host range/E.coli plasmid vector pMMB67HE - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pJF118EH, pJF118HE from pMMB22 & pKK223-3
RC pMMB66EH from RSF1010 & pJF118EH
RC pMMB66HE from pMMB66EH & pJF118HE
RC pJF119EH from pJF118EH & M13mp18
RC pJF119HE from pJF118HE & M13mp18
RC pMMB67EH from pMMB66EH & M13mp18
RC pMMB67HE from pMMB66HE & M13mp18
RC pMS324a from R300B & RP4
RC pFG101 series from pJF118EH & pMS324a
RC pFG102 series from pJF118HE & pMS324a
RC pWP101 series from pMMB66EH & pMS324a
RC pWP102 series from pMMB66HE & pMS324a
RA Furste J.P., Pansegrau W., Frank R., Blocker H., Scholz P.,
RA Bagdasarian M., Lanka E.;
RT "Molecular cloning of the plasmid RP4 primase region in a
RT multi-host-range tacP expression vector";
RL Gene 48:119-131(1986).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC Restriction digests of the clone give the following sizes (kb):
CC AccI--3.9, 3.0, 1.9; BamHI--8.8; HindIII/PvuII--7.6, 1.1. (ATCC
CC staff)
CC Contains the following sites separated by (bp): EcoRI - 739 - AhaIII
CC - 97 - ScaI - 112 - PvuI - 2101 - AccI - 1848 - ScaI - 55 - AccI -
CC 2039 - BstEII - 372 - PvuII - 93 - PvuII - 94 - HpaI - 320 - BstEII
CC - 182 - MluI - 788 - EcoRI. (personal communication)
CC The M13mp9 polylinker of pMMB66HE (ATCC 37621) was replaced with the
CC M13mp19 polylinker.
CC In the pMMB66 (ATCC 37620, 37621) and pMMB67 (ATCC 37622, 37623)
CC vector series,the EH and HE in the name designate the orientation of
CC the multiple cloning site to the tac promoter. (personal
CC communication)
CC An autoregulated high-level expression vector utilizing the tac
CC promoter, rrnB terminators and lacIq for lac repression. Concentration
CC of induced gene product may be regulated by varying the lac inducer
CC concentration.
CC Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013
CC (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis,
CC Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative
CC bacteria.
CC Belongs to a group of vectors (ATCC 37622-37623, 77287-77292)
CC differing only in antibiotic resistance and polylinker orientation.
CC (submitted, 1992)
CC Compatible with common E.coli cloning vectors, as well as vectors
CC derived from IncP and IncW plasmids. (submitted, 1992)
CC Medium is 1227 LB plus ampicillin.
CC NM (pMMB67HE)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli HB101)(broad host range)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pMMB66HE remove HindIII-EcoRI 30bp
FT \ 6..36, MCS/8791bp
FT 2. M13mp19 HindIII-EcoRI 51bp 6235..6286, MCS
FT -> pMMB67HE 8840bp"
FT - 1..5
FT /note="pMMB66HE 1..5 5bp
FT HindIII = A^AGCTT"
FT - 6..56
FT /note="M13mp19 6235..6285 51bp
FT EcoRI = G^AATTC"
FT - 57..8842
FT /note="pMMB66HE 36..8821 8786bp"
FT misc_binding 0..0
FT /note="MCS HindIII-SphI-PstI-SalI-AccI-HincII-XbaI-
FT BamHI-SmaI-XmaI-KpnI-SacI-EcoRI"
FT misc_binding 0..0
FT /note="SIT unique HindIII-SphI-PstI-SalI-AccI-
FT HincII-XbaI-BamHI-SmaI-XmaI-KpnI-SacI-EcoRI"
FT rep_origin 0..0
FT /note="ORI E. coli IncQ plasmid RSF1010 oriT and oriV"
FT promoter 0..0
FT /note="PRO E. coli tac"
FT CDS 0..0
FT /note="REP E. coli lacIq repressor gene"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
XX
SQ Sequence 8842 BP; 1914 A; 2583 C; 2639 G; 1706 T; 0 other;
aattaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt
cagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa
cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct
gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc
catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt
tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
ggtaactgtc agaccaagtt tactcatata tactttagat tgatttctga aagcgaccag
gtgctcggcg tggcaagact cgcagcgaac ccgtagaaag ccatgctcca gccgcccgca
ttggagaaat tcttcaaatt cccgttgcac atagcccggc aattcctttc cctgctctgc
cataagcgca gcgaatgccg ggtaatactc gtcaacgatc tgatagagaa gggtttgctc
gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg ctggccgtcc tggccgccac
atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat acagtctatc gcttagcgga
aagttctttt accctcagcc gaaatgcctg ccgttgctag acattgccag ccagtgcccg
tcactcccgt actaactgtc acgaacccct gcaataactg tcacgccccc ctgcaataac
tgtcacgaac ccctgcaata actgtcacgc ccccaaacct gcaaacccag caggggcggg
ggctggcggg gtgttggaaa aatccatcca tgattatcta agaataatcc actaggcgcg
gttatcagcg cccttgtggg gcgctgctgc ccttgcccaa tatgcccggc cagaggccgg
atagctggtc tattcgctgc gctaggctac acaccgcccc accgctgcgc ggcaggggga
aaggcgggca aagcccgcta aaccccacac caaaccccgc agaaatacgc tggagcgctt
ttagccgctt tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt gcagccccgc
agggcctgtc tcggtcgatc attcagcccg gctcatcctt ctggcgtggc ggcagaccga
acaaggcgcg gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg agccgatcct
ccggccactc gctgctgttc accttggcca aaatcatggc ccccaccagc accttgcgcc
ttgtttcgtt cttgcgctct tgctgctgtt cccttgcccg ctcccgctga atttcggcat
tgattcgcgc tcgttgttct tcgagcttgg ccagccgatc cgccgccttg ttgctcccct
taaccatctt gacaccccat tgttaatgtg ctgtctcgta ggctatcatg gaggcacagc
ggcggcaatc ccgaccctac tttgtagggg agggcgcact taccggtttc tcttcgagaa
actggcctaa cggccaccct tcgggcggtg cgctctccga gggccattgc atggagccga
aaagcaaaag caacagcgag gcagcatggc gatttatcac cttacggcga aaaccggcag
caggtcgggc ggccaatcgg ccagggccaa ggccgactac atccagcgcg aaggcaagta
tgcccgcgac atggatgaag tcttgcacgc cgaatccggg cacatgccgg agttcgtcga
gcggcccgcc gactactggg atgctgccga cctgtatgaa cgcgccaatg ggcggctgtt
caaggaggtc gaatttgccc tgccggtcga gctgaccctc gaccagcaga aggcgctggc
gtccgagttc gcccagcacc tgaccggtgc cgagcgcctg ccgtatacgc tggccatcca
tgccggtggc ggcgagaacc cgcactgcca cctgatgatc tccgagcgga tcaatgacgg
catcgagcgg cccgccgctc agtggttcaa gcggtacaac ggcaagaccc cggagaaggg
cggggcacag aagaccgaag cgctcaagcc caaggcatgg cttgagcaga cccgcgaggc
atgggccgac catgccaacc gggcattaga gcgggctggc cacgacgccc gcattgacca
cagaacactt gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg ggccgaacgt
ggtggagatg gaaggccggg gcatccgcac cgaccgggca gacgtggccc tgaacatcga
caccgccaac gcccagatca tcgacttaca ggaataccgg gaggcaatag accatgaacg
caatcgacag agtgaagaaa tccagaggca tcaacgagtt agcggagcag atcgaaccgc
tggcccagag catggcgaca ctggccgacg aagcccggca ggtcatgagc cagaccaagc
aggccagcga ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca ggggcggcat
gggtggagct ggccaaagag ttgcgggagg tagccgccga ggtgagcagc gccgcgcaga
gcgcccggag cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg atgctggctt
ccatgatgcc tacggtggtg ctgctgatcg catcgttgct cttgctcgac ctgacgccac
tgacaaccga ggacggctcg atctggctgc gcttggtggc ccgatgaaga acgacaggac
tttgcaggcc ataggccgac agctcaaggc catgggctgt gagcgcttcg atatcggcgt
cagggacgca cccaccggcc agatgatgaa ccgggaatgg tcagccgccg aagtgctcca
gaacacgcca tggctcaagc ggatgaatgc ccagggcaat gacgtgtata tcaggcccgc
cgagcaggag cggcatggtc tggtgctggt ggacgacctc agcgagtttg acctggatga
catgaaagcc gagggccggg agcctgccct ggtagtggaa accagcccga agaactatca
ggcatgggtc aaggtggccg acgccgcagg cggtgaactt cgggggcaga ttgcccggac
gctggccagc gagtacgacg ccgacccggc cagcgccgac agccgccact atggccgctt
ggcgggcttc accaaccgca aggacaagca caccacccgc gccggttatc agccgtgggt
gctgctgcgt gaatccaagg gcaagaccgc caccgctggc ccggcgctgg tgcagcaggc
tggccagcag atcgagcagg cccagcggca gcaggagaag gcccgcaggc tggccagcct
cgaactgccc gagcggcagc ttagccgcca ccggcgcacg gcgctggacg agtaccgcag
cgagatggcc gggctggtca agcgcttcgg tcatgacctc agcaagtgcg actttatcgc
cgcgcagaag ctggccagcc ggggccgcag tgccgaggaa atcggcaagg ccatggccga
ggccagccca gcgctggcag agcgcaagcc cggccacgaa gcggattaca tcgagcgcac
cgtcagcaag gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc tggcacgggc
accggcaccc cgccagcgag gcatggacag gggcgggcca gatttcagca tgtagtgctt
gcgttggtac tcacgcctgt tatactatga gtactcacgc acagaagggg gttttatgga
atacgaaaaa agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg gctattggtt
gcccggtggc tttggttata cgtcaaacaa ggccgaggct ggccgctttt cagtcgctga
tatggccagc cttaaccttg acggctgcac cttgtccttg ttccgcgaag acaagccttt
cggccccggc aagtttctcg gtgactgata tgaaagacca aaaggacaag cagaccggcg
acctgctggc cagccctgac gctgtacgcc aagcgcgata tgccgagcgc atgaaggcca
aagggatgcg tcagcgcaag ttctggctga ccgacgacga atacgaggcg ctgcgcgagt
gcctggaaga actcagagcg gcgcagggcg ggggtagtga ccccgccagc gcctaaccac
caactgcctg caaaggaggc aatcaatggc tacccataag cctatcaata ttctggaggc
gttcgcagca gcgccgccac cgctggacta cgttttgccc aacatggtgg ccggtacggt
cggggcgctg gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc aactggccgc
acagattgca ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg gcccggtgat
ctacctgccc gccgaagacc cgcccaccgc cattcatcac cgcctgcacg cccttggggc
gcacctcagc gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc agccgctgat
cggcagcctg cccaacatca tggccccgga gtggttcgac ggcctcaagc gcgccgccga
gggccgccgc ctgatggtgc tggacacgct gcgccggttc cacatcgagg aagaaaacgc
cagcggcccc atggcccagg tcatcggtcg catggaggcc atcgccgccg ataccgggtg
ctctatcgtg ttcctgcacc atgccagcaa gggcgcggcc atgatgggcg caggcgacca
gcagcaggcc agccggggca gctcggtact ggtcgataac atccgctggc agtcctacct
gtcgagcatg accagcgccg aggccgagga atggggtgtg gacgacgacc agcgccggtt
cttcgtccgc ttcggtgtga gcaaggccaa ctatggcgca ccgttcgctg atcggtggtt
caggcggcat gacggcgggg tgctcaagcc cgccgtgctg gagaggcagc gcaagagcaa
gggggtgccc cgtggtgaag cctaagaaca agcacagcct cagccacgtc cggcacgacc
cggcgcactg tctggccccc ggcctgttcc gtgccctcaa gcggggcgag cgcaagcgca
gcaagctgga cgtgacgtat gactacggcg acggcaagcg gatcgagttc agcggcccgg
agccgctggg cgctgatgat ctgcgcatcc tgcaagggct ggtggccatg gctgggccta
atggcctagt gcttggcccg gaacccaaga ccgaaggcgg acggcagctc cggctgttcc
tggaacccaa gtgggaggcc gtcaccgctg aatgccatgt ggtcaaaggt agctatcggg
cgctggcaaa ggaaatcggg gcagaggtcg atagtggtgg ggcgctcaag cacatacagg
actgcatcga gcgcctttgg aaggtatcca tcatcgccca gaatggccgc aagcggcagg
ggtttcggct gctgtcggag tacgccagcg acgaggcgga cgggcgcctg tacgtggccc
tgaacccctt gatcgcgcag gccgtcatgg gtggcggcca gcatgtgcgc atcagcatgg
acgaggtgcg ggcgctggac agcgaaaccg cccgcctgct gcaccagcgg ctgtgtggct
ggatcgaccc cggcaaaacc ggcaaggctt ccatagatac cttgtgcggc tatgtctggc
cgtcagaggc cagtggttcg accatgcgca agcgccgcaa gcgggtgcgc gaggcgttgc
cggagctggt cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag tacgacatca
cccggcccaa ggcggcaggc tgaccccccc cactctattg taaacaagac atttttatct
tttatattca atggcttatt ttcctgctaa ttggtaatac catgaaaaat accatgctca
gaaaaggctt aacaatattt tgaaaaattg cctactgagc gctgccgcac agctccatag
gccgctttcc tggctttgct tccagatgta tgctcttctg ctcccgaacg ccagcaagac
gtagcccagc gcgtcggcca gcttgcaatt cgcgctaact tacattaatt gcgttgcgct
cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag
acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc
acgtggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc
cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
tgcaccattc gatggtgtca acgtaaatgc cgcttcgcct tcgcgcgcga attgcaagct
gatccgggct tatcgactgc acggtgcacc aatgcttctg gcgtcaggca gccatcggaa
gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac
tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa atattctgaa
atgagctgtt gacaattaat catcggctcg tataatgtgt ggaattgtga gcggataaca
atttcacaca ggaaacagaa tt
//