back Return to this vector's summary.
ID   PMMB7      preliminary; circular DNA; SYN; 8693 BP.
XX
AC   IG9893;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Broad host range/E.coli cosmid vector pMMB7 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pMMB7 from RSF1010 & linker
RC   pMMB31 from pKT231 & pHC79
RC   pMMB33, pMMB34 from pMMB7 & pMMB31
RA   Frey J., Bagdasarian M., Feiss D., Franklin F.C., Deshusses J.;
RT   "Stable cosmid vectors that enable the introduction of cloned
RT   fragments into a wide range of gram-negative bacteria";
RL   Gene 24:299-308(1983).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pMMB7)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli C600)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (RSF1010)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. RSF1010 HpaI 8684bp 1..1
FT                   2. linker 9bp ccggatcgg
FT                   -> pMMB7 8693bp [BamHI in sulf/spec genes]"
FT   -               1..9
FT                   /note="ccggatcgg 9bp
FT                   \ ccggatcgg
FT                   HpaI =  GTT^AAC"
FT   -               10..8693
FT                   /note="RSF1010 1..8684 8684bp
FT                   HpaI = GTT^AAC
FT                   \          ccggatcgg"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-SstI-EcoRI-HpaI"
FT   rep_origin      0..0
FT                   /note="ORI Pseudomonas R300B"
FT   CDS             0..0
FT                   /note="ANT Pseudomonas kanamycin resistance gene
FT                   (kan)"
XX
SQ   Sequence 8693 BP; 1758 A; 2630 C; 2671 G; 1634 T; 0 other;
     ccggatcgga actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct gactggttgc
     ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg
     cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt
     ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg
     gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg
     atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg
     ttgatcaatg tccgtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg
     tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgctgc
     acgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg
     atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc
     ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt
     tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg
     ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct
     tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc
     tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa
     agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat
     tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg
     gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc
     tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc
     gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg
     ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta
     cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct
     acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga
     tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc
     tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc
     tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct
     ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc
     gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct atcccctggg
     aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg attccgcccc
     ttcccgcaaa cgaccccaaa caggaaacgc agctgaaacg ggaagctcaa cacccactga
     cgcatgggtt gttcaggcag tacttcatca accagcaagg cggcactttc ggccatccgc
     cgcgccccac agctcgggca gaaaccgcga cgcttacagc tgaaagcgac caggtgctcg
     gcgtggcaag actcgcagcg aacccgtaga aagccatgct ccagccgccc gcattggaga
     aattcttcaa attcccgttg cacatagccc ggcaattcct ttccctgctc tgccataagc
     gcagcgaatg ccgggtaata ctcgtcaacg atctgataga gaagggtttg ctcgggtcgg
     tggctctggt aacgaccagt atcccgatcc cggctggccg tcctggccgc cacatgaggc
     atgttccgcg tccttgcaat actgtgttta catacagtct atcgcttagc ggaaagttct
     tttaccctca gccgaaatgc ctgccgttgc tagacattgc cagccagtgc ccgtcactcc
     cgtactaact gtcacgaacc cctgcaataa ctgtcacgcc cccctgcaat aactgtcacg
     aacccctgca ataactgtca cgcccccaaa cctgcaaacc cagcaggggc gggggctggc
     ggggtgttgg aaaaatccat ccatgattat ctaagaataa tccactaggc gcggttatca
     gcgcccttgt ggggcgctgc tgcccttgcc caatatgccc ggccagaggc cggatagctg
     gtctattcgc tgcgctaggc tacacaccgc cccaccgctg cgcggcaggg ggaaaggcgg
     gcaaagcccg ctaaacccca caccaaaccc cgcagaaata cgctggagcg cttttagccg
     ctttagcggc ctttccccct acccgaaggg tgggggcgcg tgtgcagccc cgcagggcct
     gtctcggtcg atcattcagc ccggctcatc cttctggcgt ggcggcagac cgaacaaggc
     gcggtcgtgg tcgcgttcaa ggtacgcatc cattgccgcc atgagccgat cctccggcca
     ctcgctgctg ttcaccttgg ccaaaatcat ggcccccacc agcaccttgc gccttgtttc
     gttcttgcgc tcttgctgct gttcccttgc ccgcacccgc tgaatttcgg cattgattcg
     cgctcgttgt tcttcgagct tggccagccg atccgccgcc ttgttgctcc ccttaaccat
     cttgacaccc cattgttaat gtgctgtctc gtaggctatc atggaggcac agcggcggca
     atcccgaccc tactttgtag gggagggcgc acttaccggt ttctcttcga gaaactggcc
     taacggccac ccttcgggcg gtgcgctctc cgagggccat tgcatggagc cgaaaagcaa
     aagcaacagc gaggcagcat ggcgatttat caccttacgg cgaaaaccgg cagcaggtcg
     ggcggccaat cggccagggc caaggccgac tacatccagc gcgaaggcaa gtatgcccgc
     gacatggatg aagtcttgca cgccgaatcc gggcacatgc cggagttcgt cgagcggccc
     gccgactact gggatgctgc cgacctgtat gaacgcgcca atgggcggct gttcaaggag
     gtcgaatttg ccctgccggt cgagctgacc ctcgaccagc agaaggcgct ggcgtccgag
     ttcgcccagc acctgaccgg tgccgagcgc ctgccgtata cgctggccat ccatgccggt
     ggcggcgaga acccgcactg ccacctgatg atctccgagc ggatcaatga cggcatcgag
     cggcccgccg ctcagtggtt caagcggtac aacggcaaga ccccggagaa gggcggggca
     cagaagaccg aagcgctcaa gcccaaggca tggcttgagc agacccgcga ggcatgggcc
     gaccatgcca accgggcatt agagcgggct ggccacgacg cccgcattga ccacagaaca
     cttgaggcgc agggcatcga gcgcctgccc ggtgttcacc tggggccgaa cgtggtggag
     atggaaggcc ggggcatccg caccgaccgg gcagacgtgg ccctgaacat cgacaccgcc
     aacgcccaga tcatcgactt acaggaatac cgggaggcaa tagaccatga acgcaatcga
     cagagtgaag aaatccagag gcatcaacga gttagcggag cagatcgaac cgctggccca
     gagcatggcg acactggccg acgaagcccg gcaggtcatg agccagaccc agcaggccag
     cgaggcgcag gcggcggagt ggctgaaagc ccagcgccag acaggggcgg catgggtgga
     gctggccaaa gagttgcggg aggtagccgc cgaggtgagc agcgccgcgc agagcgcccg
     gagcgcgtcg cgggggtggc actggaagct atggctaacc gtgatgctgg cttccatgat
     gcctacggtg gtgctgctga tcgcatcgtt gctcttgctc gacctgacgc cactgacaac
     cgaggacggc tcgatctggc tgcgcttggt ggcccgatga agaacgacag gactttgcag
     gccataggcc gacagctcaa ggccatgggc tgtgagcgct tcgatatcgg cgtcagggac
     gccaccaccg gccagatgat gaaccgggaa tggtcagccg ccgaagtgct ccagaacacg
     ccatggctca agcggatgaa tgcccagggc aatgacgtgt atatcaggcc cgccgagcag
     gagcggcatg gtctggtgct ggtggacgac ctcagcgagt ttgacctgga tgacatgaaa
     gccgagggcc gggagcctgc cctggtagtg gaaaccagcc cgaagaacta tcaggcatgg
     gtcaaggtgg ccgacgccgc aggcggtgaa cttcgggggc agattgcccg gacgctggcc
     agcgagtacg acgccgaccc ggccagcgcc gacagccgcc actatggccg cttggcgggc
     ttcaccaacc gcaaggacaa gcacaccacc cgcgccggtt atcagccgtg ggtgctgctg
     cgtgaatcca agggcaagac cgccaccgct ggcccggcgc tggtgcagca ggctggccag
     cagatcgagc aggcccagcg gcagcaggag aaggcccgca ggctggccag cctcgaactg
     cccgagcggc agcttagccg ccaccggcgc acggcgctgg acgagtaccg cagcgagatg
     gccgggctgg tcaagcgctt cggtgatgac ctcagcaagt gcgactttat cgccgcgcag
     aagctggcca gccggggccg cagtgccgag gaaatcggca aggccatggc cgaggccagc
     ccagcgctgg cagagcgcaa gcccggccac gaagcggatt acatcgagcg caccgtcagc
     aaggtcatgg gtctgcccag cgtccagctt gcgcgggccg agctggcacg ggcaccggca
     ccccgccagc gaggcatgga caggggcggg ccagatttca gcatgtagtg cttgcgttgg
     tactcacgcc tgttatacta tgagtactca cgcacagaag ggggttttat ggaatacgaa
     aaaagcgctt cagggtcggt ctacctgatc aaaagtgaca agggctattg gttgcccggt
     ggctttggtt atacgtcaaa caaggccgag gctggccgct tttcagtcgc tgatatggcc
     agccttaacc ttgacggctg caccttgtcc ttgttccgcg aagacaagcc tttcggcccc
     ggcaagtttc tcggtgactg atatgaaaga ccaaaaggac aagcagaccg gcgacctgct
     ggccagccct gacgctgtac gccaagcgcg atatgccgag cgcatgaagg ccaaagggat
     gcgtcagcgc aagttctggc tgaccgacga cgaatacgag gcgctgcgcg agtgcctgga
     agaactcaga gcggcgcagg gcgggggtag tgaccccgcc agcgcctaac caccaactgc
     ctgcaaagga ggcaatcaat ggctacccat aagcctatca atattctgga ggcgttcgca
     gcagcgccgc caccgctgga ctacgttttg cccaacatgg tggccggtac ggtcggggcg
     ctggtgtcgc ccggtggtgc cggtaaatcc atgctggccc tgcaactggc cgcacagatt
     gcaggcgggc cggatctgct ggaggtgggc gaactgccca ccggcccggt gatctacctg
     cccgccgaag acccgcccac cgccattcat caccgcctgc acgcccttgg ggcgcacctc
     agcgccgagg aacggcaagc cgtggctgac ggcctgctga tccagccgct gatcggcagc
     ctgcccaaca tcatggcccc ggagtggttc gacggcctca agcgcgccgc cgagggccgc
     cgcctgatgg tgctggacac gctgcgccgg ttccacatcg aggaagaaaa cgccagcggc
     cccatggccc aggtcatcgg tcgcatggag gccatcgccg ccgataccgg gtgctctatc
     gtgttcctgc accatgccag caagggcgcg gccatgatgg gcgcaggcga ccagcagcag
     gccagccggg gcagctcggt actggtcgat aacatccgct ggcagtccta cctgtcgagc
     atgaccagcg ccgaggccga ggaatggggt gtggacgacg accagcgccg gttcttcgtc
     cgcttcggtg tgagcaaggc caactatggc gcaccgttcg ctgatcggtg gttcaggcgg
     catgacggcg gggtgctcaa gcccgccgtg ctggagaggc agcgcaagag caagggggtg
     ccccgtggtg aagcctaaga acaagcacag cctcagccac gtccggcacg acccggcgca
     ctgtctggcc cccggcctgt tccgtgccct caagcggggc gagcgcaagc gcagcaagct
     ggacgtgacg tatgactacg gcgacggcaa gcggatcgag ttcagcggcc cggagccgct
     gggcgctgat gatctgcgca tcctgcaagg gctggtggcc atggctgggc ctaatggcct
     agtgcttggc ccggaaccca agaccgaagg cggacggcag ctccggctgt tcctggaacc
     caagtgggag gccgtcaccg ctgaatgcca tgtggtcaaa ggtagctatc gggcgctggc
     aaaggaaatc ggggcagagg tcgatagtgg tggggcgctc aagcacatac aggactgcat
     cgagcgcctt tggaaggtat ccatcatcgc ccagaatggc cgcaagcggc aggggtttcg
     gctgctgtcg gagtacgcca gcgacgaggc ggacgggcgc ctgtacgtgg ccctgaaccc
     cttgatcgcg caggccgtca tgggtggcgg ccagcatgtg cgcatcagca tggacgaggt
     gcgggcgctg gacagcgaaa ccgcccgcct gctgcaccag cggctgtgtg gctggatcga
     ccccggcaaa accggcaagg cttccataga taccttgtgc ggctatgtct ggccgtcaga
     ggccagtggt tcgaccatgc gcaagcgccg ccagcgggtg cgcgaggcgt tgccggagct
     ggtcgcgctg ggctggacgg taaccgagtt cgcggcgggc aagtacgaca tcacccggcc
     caaggcggca ggctgacccc ccccactcta ttgtaaacaa gacattttta tcttttatat
     tcaatggctt attttcctgc taattggtaa taccatgaaa aataccatgc tcagaaaagg
     cttaacaata ttttgaaaaa ttgcctactg agcgctgccg cacagctcca taggccgctt
     tcctggcttt gcttccagat gtatgctctt ctgctcctgc agctaatgga tcaccgcaaa
     caggttactc gcctggggat tccctttcga cccgagcatc cgtatgatac tcatgctcga
     ttattattat tatagaagcc cccatgaata aatcgctcat cattttcggc atcgtcaaca
     taacctcgga cagtttctcc gatggaggcc ggtatctggc gccagacgca gccattgcgc
     aggcgcgtaa gctgatggcc gagggggcag atgtgatcga cctggtccgg catccagcaa
     tcccgacgcc gcgcctgttt cgtccgacac agaaatcgcg cgtatgcgcc ggtgctggac
     gcgctcaggc agatggcatt cccgtctcgc tcgacagtta tcaacccgcg acgcaagcct
     atgccttgtc gcgtggtgtg gcctatctca atgatattcg cggttttcca gacgctgcgt
     tctatccgca attggcgaaa tcatctgcca aactcgtcgt tatgcattcg gtgcaagacg
     ggcaggcaga tcggcgcgag gcacccgctg gcgacatcat ggatcacatt gcggcgttct
     ttgacgcgcg catcgcggcg ctgacgggtg ccggtatcaa acgcaaccgc cttgtccttg
     atcccggcat ggggtttttt ctgggggctg ctcccgaaac ctcgctctcg gtgctggcgc
     ggttcgatga attgcggctg cgcttcgatt tgccggtgct tctgtctgtt tcgcgcaaat
     cctttctgcg cgcgctcaca ggccgtggtc cgggggtgtc ggggccgcga cactcgctgc
     agagcttgcc gccgccgcag gtggagctga cttcatccgc acacacgagc cgcgcccctt
     gcgcgacggg ctggcggtat tggcggcgct gaaagaaacc gcaagaattc gtt
//