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ID PMSGCAT preliminary; circular DNA; SYN; 8405 BP.
XX
AC IG1094;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pMSG-CAT - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC plasmid from pBR322 & adenovirus 5 DBP gene
RC pMSG-DBP1 from pMSG or pMDSG & plasmid, DBP gene
RA Klessig D.F., Brough D.E., Cleghon V.;
RT "Introduction, stable integration, and controlled expression of a
RT chimeric adenovirus gene whose product is toxic to the recipient
RT human cell";
RL Mol. Cell. Biol. 4:1354-1362(1984).
XX
CC mouse mammary tumor virus long terminal repeat is a glucocorticoid
CC -inducible promoter, e.g., by dexamethasone.
CC uses first ATG in inserted DNA.
CC NM (pMSG-CAT)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Pharmacia)
CC HO (E.coli HB101)(hormonally responsive mammal)
CC HO (Chinese hamster ovary CHO)(human HeLa)(mouse Ltk-)(mouse 3T6)
CC CP ()
CC FN (expression shuttle)(expression inducible)
CC SE ()
CC PA (pMSG)(Tn9)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pMSG SalI 7626bp 13..13
FT 2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT -> pMSG-CAT 8405bp"
FT misc_binding 1..6
FT /note="MCS unique NheI-SmaI"
FT misc_binding 12..12
FT /note="SIT double SalI"
FT CDS 12..791
FT /note="ANT E. coli chloramphenicol acetyltransferase
FT gene (CAT) chloramphenicol resistance gene (cmr/cml)"
FT misc_binding 791..791
FT /note="SIT double SalI"
FT misc_binding 797..797
FT /note="SIT double XhoI"
FT misc_feature >797..<1517
FT /note="SPL SV40 early genes"
FT polyA_signal >1517..<1650
FT /note="PLA SV40 early genes"
FT rep_origin 1650..1996
FT /note="ORI SV40"
FT promoter 1650..1996
FT /note="PRO SV40 early genes"
FT CDS 1996..<3767
FT /note="GEN E. coli xanthine-guanine
FT phosphoribosyl-transferase (gpt) gene"
FT misc_binding 2116..2116
FT /note="SIT unique BglII"
FT misc_binding 2314..2314
FT /note="SIT unique KpnI"
FT misc_binding 2576..2576
FT /note="SIT unique KpnI"
FT misc_feature 0..0
FT /note="SPL SV40 early genes"
FT polyA_signal 0..0
FT /note="PLA SV40 early genes"
FT CDS >4651..<6100
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 5277..5277
FT /note="SIT unique PvuI"
FT rep_origin >5500..<6950
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_binding 6950..6950
FT /note="SIT HindIII"
FT promoter 6950..8405
FT /note="PRO mouse mammary tumor virus (MMTV)
FT long terminal repeat (LTR), PstI-PstI fragment"
FT misc_binding 7277..7277
FT /note="SIT unique ClaI"
FT misc_binding 8030..8030
FT /note="SIT unique SacI"
XX
SQ Sequence 8405 BP; 2293 A; 1899 C; 1876 G; 2337 T; 0 other;
gctagcccgg ggtcgacgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt
aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
tattggtgcc cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaatg
gcagaaattc gtcgacctcg agggatcttt gtgaaggaac cttacttctg tggtgtgaca
taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
caaactcatc aatgtatctt atcatgtctg gatccgctgt ggaatgtgtg tcagttaggg
tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc
actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact
ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt
ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg
ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg
gaaaggcatt attgccgtaa gccgtggcgg tctggtaccg ggtgcgttac tggcgcgtga
actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
gatccccagg aagctcctct gtgtcctcat aaaccctaac ctcctctact tgagaggaca
ttccaatcat aggctgccca tccaccctct gtgtcctcct gttaattagg tcacttaaca
aaaaggaaat tgggtagggg tttttcacag accgctttct aagggtaatt ttaaaatatc
tgggaagtcc cttccactgc tgtgttccag aagtgttggt aaacagccca caaatgtcaa
cagcagaaac atacaagctg tcagctttgc acaagggccc aacaccctgc tcatcaagaa
gcactgtggt tgctgtgtta gtaatgtgca aaacaggagg cacattttcc ccacctgtgt
aggttccaaa atatctagtg ttttcatttt tacttggatc aggaacccag cactccactg
gataagcatt atccttatcc aaaacagcct tgtggtcagt gttcatctgc tgactgtcaa
ctgtagcatt ttttggggtt acagtttgag caggatattt ggtcctgtag tttgctaaca
caccctgcag ctccaaaggt tccccaccaa cagcaaaaaa atgaaaattt gacccttgaa
tgggttttcc agcaccattt tcatgagttt tttgtgtccc tgaatgcaag tttaacatag
cagttacccc aataacctca gttttaacag taacagcttc ccacatcaaa atatttccac
aggttaagtc ctcatttaaa ttaggcaaag gaattcttga agacgaaagg gcctcgtgat
acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagca agcttggcag
aaatggttga actcccgaga gtgtcctaca cctaggggag aagcagccaa ggggttgttt
cccaccaagg acgacccgtc tgcgcacaaa cggatgagcc catcagacaa agacatattc
attctctgct gcaaacttgg catagctctg ctttgcctgg ggctattggg ggaagttgcg
gttcgtgctc gcagggctct cacccttgac tctttcaata ataactcttc tgtgcaagat
tacaatctaa acaattcgga gaactcgacc ttcctcctga ggcaaggacc acagccaact
tcctcttaca agccgcatcg attttgtcct tcagaaatag aaataagaat gcttgctaaa
aattatattt ttaccaataa gaccaatcca ataggtagat tattagttac tatgttaaga
aatgaatcat tatcttttag tactattttt actcaaattc agaagttaga aatgggaata
gaaaatagaa agagacgctc aacctcaatt gaagaacagg tgcaaggact attgaccaca
ggcctagaag taaaaaaggg aaaaaagagt gtttttgtca aaataggaga caggtggtgg
caaccaggga cttatagggg accttacatc tacagaccaa cagatgcccc cttaccatat
acaggaagat atgacttaaa ttgggatagg tgggttacag tcaatggcta taaagtgtta
tatagatccc tcccctttcg tgaaagactc gccagagcta gacctccttg gtgtatgttg
tctcaagaaa agaaagacga catgaaacaa caggtacatg attatattta tctaggaaca
ggaatgcact tttggggaaa gattttccat accaaggagg ggacagtggc tggactaata
gaacattatt ctgcaaaaac ttatggcatg agttattatg attagccttg atttgcccaa
ccttgcggtt cccaaggctt aagtaagttt ttggttacaa actgttctta aaacaaggat
gtgagacaag tggtttcctg acttggtttg gtatcaaagg ttctgatctg agctctgagt
gttctatttt cctatgttct tttggaattt atccaaatct tatgtaaatg cttatgtaaa
ccaagatata aaagagtgct gattttttga gtaaacttgc aacagtccta acattcacct
cttgtgtgtt tgtgtctgtt cgccatcccg tctccgctcg tcacttatcc ttcactttcc
agagggtccc cccgcagacc ccggcgaccc tcaggtcggc cgactgcggc agctggcgcc
cgaacaggga ccctcggata agtgaccctt gtctttattt ctactatttt gtgttcgtct
tgttttgtct ctatcttgtc tggctatcat cacaagagcg gaacggactc acctcaggga
accaa
//