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ID   PMSGCAT    preliminary; circular DNA; SYN; 8405 BP.
XX
AC   IG1094;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMSG-CAT - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from pBR322 & adenovirus 5 DBP gene
RC   pMSG-DBP1 from pMSG or pMDSG & plasmid, DBP gene
RA   Klessig D.F., Brough D.E., Cleghon V.;
RT   "Introduction, stable integration, and controlled expression of a
RT   chimeric adenovirus gene whose product is toxic to the recipient
RT   human cell";
RL   Mol. Cell. Biol. 4:1354-1362(1984).
XX
CC   mouse mammary tumor virus long terminal repeat is a glucocorticoid
CC   -inducible promoter, e.g., by dexamethasone.
CC   uses first ATG in inserted DNA.
CC   NM (pMSG-CAT)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli HB101)(hormonally responsive mammal)
CC   HO (Chinese hamster ovary CHO)(human HeLa)(mouse Ltk-)(mouse 3T6)
CC   CP ()
CC   FN (expression shuttle)(expression inducible)
CC   SE ()
CC   PA (pMSG)(Tn9)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMSG SalI 7626bp 13..13
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pMSG-CAT 8405bp"
FT   misc_binding    1..6
FT                   /note="MCS unique NheI-SmaI"
FT   misc_binding    12..12
FT                   /note="SIT double SalI"
FT   CDS             12..791
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (cmr/cml)"
FT   misc_binding    791..791
FT                   /note="SIT double SalI"
FT   misc_binding    797..797
FT                   /note="SIT double XhoI"
FT   misc_feature    >797..<1517
FT                   /note="SPL SV40 early genes"
FT   polyA_signal    >1517..<1650
FT                   /note="PLA SV40 early genes"
FT   rep_origin      1650..1996
FT                   /note="ORI SV40"
FT   promoter        1650..1996
FT                   /note="PRO SV40 early genes"
FT   CDS             1996..<3767
FT                   /note="GEN E. coli xanthine-guanine
FT                   phosphoribosyl-transferase (gpt) gene"
FT   misc_binding    2116..2116
FT                   /note="SIT unique BglII"
FT   misc_binding    2314..2314
FT                   /note="SIT unique KpnI"
FT   misc_binding    2576..2576
FT                   /note="SIT unique KpnI"
FT   misc_feature    0..0
FT                   /note="SPL SV40 early genes"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   CDS             >4651..<6100
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    5277..5277
FT                   /note="SIT unique PvuI"
FT   rep_origin      >5500..<6950
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    6950..6950
FT                   /note="SIT HindIII"
FT   promoter        6950..8405
FT                   /note="PRO mouse mammary tumor virus (MMTV)
FT                   long terminal repeat (LTR), PstI-PstI fragment"
FT   misc_binding    7277..7277
FT                   /note="SIT unique ClaI"
FT   misc_binding    8030..8030
FT                   /note="SIT unique SacI"
XX
SQ   Sequence 8405 BP; 2293 A; 1899 C; 1876 G; 2337 T; 0 other;
     gctagcccgg ggtcgacgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt
     aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     tattggtgcc cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaatg
     gcagaaattc gtcgacctcg agggatcttt gtgaaggaac cttacttctg tggtgtgaca
     taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
     aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
     attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
     aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
     agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     caaactcatc aatgtatctt atcatgtctg gatccgctgt ggaatgtgtg tcagttaggg
     tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc
     actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact
     ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt
     ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg
     ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg
     gaaaggcatt attgccgtaa gccgtggcgg tctggtaccg ggtgcgttac tggcgcgtga
     actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
     cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
     tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
     cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
     tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
     acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
     gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     gatccccagg aagctcctct gtgtcctcat aaaccctaac ctcctctact tgagaggaca
     ttccaatcat aggctgccca tccaccctct gtgtcctcct gttaattagg tcacttaaca
     aaaaggaaat tgggtagggg tttttcacag accgctttct aagggtaatt ttaaaatatc
     tgggaagtcc cttccactgc tgtgttccag aagtgttggt aaacagccca caaatgtcaa
     cagcagaaac atacaagctg tcagctttgc acaagggccc aacaccctgc tcatcaagaa
     gcactgtggt tgctgtgtta gtaatgtgca aaacaggagg cacattttcc ccacctgtgt
     aggttccaaa atatctagtg ttttcatttt tacttggatc aggaacccag cactccactg
     gataagcatt atccttatcc aaaacagcct tgtggtcagt gttcatctgc tgactgtcaa
     ctgtagcatt ttttggggtt acagtttgag caggatattt ggtcctgtag tttgctaaca
     caccctgcag ctccaaaggt tccccaccaa cagcaaaaaa atgaaaattt gacccttgaa
     tgggttttcc agcaccattt tcatgagttt tttgtgtccc tgaatgcaag tttaacatag
     cagttacccc aataacctca gttttaacag taacagcttc ccacatcaaa atatttccac
     aggttaagtc ctcatttaaa ttaggcaaag gaattcttga agacgaaagg gcctcgtgat
     acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagca agcttggcag
     aaatggttga actcccgaga gtgtcctaca cctaggggag aagcagccaa ggggttgttt
     cccaccaagg acgacccgtc tgcgcacaaa cggatgagcc catcagacaa agacatattc
     attctctgct gcaaacttgg catagctctg ctttgcctgg ggctattggg ggaagttgcg
     gttcgtgctc gcagggctct cacccttgac tctttcaata ataactcttc tgtgcaagat
     tacaatctaa acaattcgga gaactcgacc ttcctcctga ggcaaggacc acagccaact
     tcctcttaca agccgcatcg attttgtcct tcagaaatag aaataagaat gcttgctaaa
     aattatattt ttaccaataa gaccaatcca ataggtagat tattagttac tatgttaaga
     aatgaatcat tatcttttag tactattttt actcaaattc agaagttaga aatgggaata
     gaaaatagaa agagacgctc aacctcaatt gaagaacagg tgcaaggact attgaccaca
     ggcctagaag taaaaaaggg aaaaaagagt gtttttgtca aaataggaga caggtggtgg
     caaccaggga cttatagggg accttacatc tacagaccaa cagatgcccc cttaccatat
     acaggaagat atgacttaaa ttgggatagg tgggttacag tcaatggcta taaagtgtta
     tatagatccc tcccctttcg tgaaagactc gccagagcta gacctccttg gtgtatgttg
     tctcaagaaa agaaagacga catgaaacaa caggtacatg attatattta tctaggaaca
     ggaatgcact tttggggaaa gattttccat accaaggagg ggacagtggc tggactaata
     gaacattatt ctgcaaaaac ttatggcatg agttattatg attagccttg atttgcccaa
     ccttgcggtt cccaaggctt aagtaagttt ttggttacaa actgttctta aaacaaggat
     gtgagacaag tggtttcctg acttggtttg gtatcaaagg ttctgatctg agctctgagt
     gttctatttt cctatgttct tttggaattt atccaaatct tatgtaaatg cttatgtaaa
     ccaagatata aaagagtgct gattttttga gtaaacttgc aacagtccta acattcacct
     cttgtgtgtt tgtgtctgtt cgccatcccg tctccgctcg tcacttatcc ttcactttcc
     agagggtccc cccgcagacc ccggcgaccc tcaggtcggc cgactgcggc agctggcgcc
     cgaacaggga ccctcggata agtgaccctt gtctttattt ctactatttt gtgttcgtct
     tgttttgtct ctatcttgtc tggctatcat cacaagagcg gaacggactc acctcaggga
     accaa
//