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ID   PMTL20P    preliminary; circular DNA; SYN; 2854 BP.
XX
AC   M21875;
XX
DT   15-JUN-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pMTL20P - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-2854
RC   pMTL1 from pBR322
RC   pMTL2 from pMTL1
RC   pMTL3 from pMTL2 & pUC8
RC   pMTL4 from pMTL3
RC   [pMTL18 from M13mp18]
RC   [pMTL19 from M13mp19]
RC   pMTL20 from pMTL4 & M13mp18 & oligo
RC   pMTL21 from pMTL4 & M13mp19 & oligo
RC   pMTL22 from pMTL4 & M13mp18 & oligo
RC   pMTL23 from pMTL4 & M13mp19 & oligo
RC   pMTL24 from pMTL20 & pUC18
RC   pMTL25 from pMTL21 & pUC19
RC   pMTL26 from pMTL4 & M13mp19 & oligo
RC   pMTL20P from pMTL20 & pSC101, par
RC   pMTL21P from pMTL21 & pSC101, par
RC   pMTL18P from pMTL18 & pSC101, par
RC   pMTL19P from pMTL19 & pSC101, par
RC   pMTL22P from pMTL22 & pSC101, par
RC   pMTL23P from pMTL23 & pSC101, par
RC   pMTL24P from pMTL24 & pSC101, par
RC   pMTL25P from pMTL25 & pSC101, par
RC   pMTL26P from pMTL26 & pSC101, par
RA   Chambers S.P., Prior S.E., Barstow D.A., Minton N.P.;
RT   "The pMTL nic- cloning vectors, I. Improved pUC polylinker regions to
RT   facilitate the generation of sonicated DNA for nucleotide sequencing";
RL   Gene 68:139-149(1988).
XX
CC   NCBI gi: 209094
CC   NM (pMTL20P)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pMTL nic- from pUC)
CC   BR ()
CC   OF ()
CC   OR (HaeII site)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMTL20 EcoRV 2400..2400
FT                   2. pSC101 385bp, par
FT                   Klenow:Klenow
FT                   -> pMTL20P 2854bp"
FT   CDS             complement(1..259)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      complement(652..657)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1416..2276)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             complement(2850..2854)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_feature    0..0
FT                   /note="from pUC"
XX
SQ   Sequence 2854 BP; 683 A; 735 C; 710 G; 726 T; 0 other;
     gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagcttgca tgcctgcagg
     cctcgagatc tccatggacg cgtgacgtcg actctagagg atccccgggt accgagctcg
     aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     ccacctgact gccgggcctc ttgcgggata attctcgggc aaatcgctga atattccttt
     tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc
     tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg
     caaggaaact acccataata caagaaaagc ccgtcacggg ctctcagggc gttttatggc
     gggtctgcta tgtggtgcta tctgactttt tgctgttcgc agttcctgcc ctctgatttt
     ccagtctgac cacttcggat tatcccgtga caggtcattc agactggcta atgcacccag
     taaggcagcg gtatcatcaa caggcttacc cgtcttactg tcgaattatc gtccattccg
     acagcatcgc cagtcactat ggcgtgctgc tagc
//