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ID   PMTL3      preliminary; circular DNA; SYN; 2246 BP.
XX
AC   IG9897;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMTL3 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pMTL1 from pBR322
RC   pMTL2 from pMTL1
RC   pMTL3 from pMTL2 & pUC8
RC   pMTL4 from pMTL3
RC   [pMTL18 from M13mp18]
RC   [pMTL19 from M13mp19]
RC   pMTL20 from pMTL4 & M13mp18 & oligo
RC   pMTL21 from pMTL4 & M13mp19 & oligo
RC   pMTL22 from pMTL4 & M13mp18 & oligo
RC   pMTL23 from pMTL4 & M13mp19 & oligo
RC   pMTL24 from pMTL20 & pUC18
RC   pMTL25 from pMTL21 & pUC19
RC   pMTL26 from pMTL4 & M13mp19 & oligo
RC   pMTL20P from pMTL20 & pSC101, par
RC   pMTL21P from pMTL21 & pSC101, par
RC   pMTL18P from pMTL18 & pSC101, par
RC   pMTL19P from pMTL19 & pSC101, par
RC   pMTL22P from pMTL22 & pSC101, par
RC   pMTL23P from pMTL23 & pSC101, par
RC   pMTL24P from pMTL24 & pSC101, par
RC   pMTL25P from pMTL25 & pSC101, par
RC   pMTL26P from pMTL26 & pSC101, par
RA   Chambers S.P., Prior S.E., Barstow D.A., Minton N.P.;
RT   "The pMTL nic- cloning vectors, I. Improved pUC polylinker regions to
RT   facilitate the generation of sonicated DNA for nucleotide sequencing";
RL   Gene 68:139-149(1988).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pMTL3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(pUC8)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove BamHI-PvuII 1691bp 376..2067,
FT                   \ tet/2670bp
FT                   Klenow:
FT                   -> pMTL1 2670bp [unique BamHI]
FT                   1. pMTL1 remove HaeII-HaeII, rop/nic
FT                   \ pBR322 237..376..-2067..2352
FT                   -> pMTL2 2048bp [no NdeI at 2297]
FT                   1. pMTL2 remove TaqI-TaqI 1444bp, PstI/HincII in amp
FT                   \ pBR322 2574..4018
FT                   2. pUC8 TaqI-TaqI 1444bp 998..2442, amp gene
FT                   -> pMTL3 2250bp"
FT   -               1..236
FT                   /note="pBR322 1..236 236bp
FT                   HaeII = RGCGC^Y"
FT   -               237..458
FT                   /note="pBR322 2352..2573 222bp
FT                   TaqI = T^CGA"
FT   -               459..1902
FT                   /note="pUC8 998..2441 1444bp
FT                   TaqI = T^CGA"
FT   -               1903..2246
FT                   /note="pBR322 4018..4361 344bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2246 BP; 598 A; 551 C; 549 G; 548 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctctt
     ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     tccataggct ccgcccccct gacgagcatc acaaaaatcg aactggatct caacagcggt
     aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgatgtaa cccactcgtg
     cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     tcacgaggcc ctttcgtctt caagaa
//