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ID   PMTL4      preliminary; circular DNA; SYN; 2010 BP.
XX
AC   IG9898;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pMTL4 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pMTL1 from pBR322
RC   pMTL2 from pMTL1
RC   pMTL3 from pMTL2 & pUC8
RC   pMTL4 from pMTL3
RC   [pMTL18 from M13mp18]
RC   [pMTL19 from M13mp19]
RC   pMTL20 from pMTL4 & M13mp18 & oligo
RC   pMTL21 from pMTL4 & M13mp19 & oligo
RC   pMTL22 from pMTL4 & M13mp18 & oligo
RC   pMTL23 from pMTL4 & M13mp19 & oligo
RC   pMTL24 from pMTL20 & pUC18
RC   pMTL25 from pMTL21 & pUC19
RC   pMTL26 from pMTL4 & M13mp19 & oligo
RC   pMTL20P from pMTL20 & pSC101, par
RC   pMTL21P from pMTL21 & pSC101, par
RC   pMTL18P from pMTL18 & pSC101, par
RC   pMTL19P from pMTL19 & pSC101, par
RC   pMTL22P from pMTL22 & pSC101, par
RC   pMTL23P from pMTL23 & pSC101, par
RC   pMTL24P from pMTL24 & pSC101, par
RC   pMTL25P from pMTL25 & pSC101, par
RC   pMTL26P from pMTL26 & pSC101, par
RA   Chambers S.P., Prior S.E., Barstow D.A., Minton N.P.;
RT   "The pMTL nic- cloning vectors, I. Improved pUC polylinker regions to
RT   facilitate the generation of sonicated DNA for nucleotide sequencing";
RL   Gene 68:139-149(1988).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pMTL4)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(pUC8)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove BamHI-PvuII 1691bp 376..2067,
FT                   \ tet/2670bp
FT                   Klenow:
FT                   -> pMTL1 2670bp [unique BamHI]
FT                   1. pMTL1 remove HaeII-HaeII, rop/nic
FT                   \ pBR322 237..376..-2067..2352
FT                   -> pMTL2 2048bp [no NdeI at 2297]
FT                   1. pMTL2 remove TaqI-TaqI 1444bp, PstI/HincII in amp
FT                   \ pBR322 2574..4018
FT                   2. pUC8 TaqI-TaqI 1444bp 998..2442, amp gene
FT                   -> pMTL3 2250bp
FT                   1. pMTL3 remove AatII-RsaI 238bp, pBR322 4289..164
FT                   T4 DNA polymerase:
FT                   -> pMTL4 1900bp"
FT   -               1..73
FT                   /note="pBR322 164..236 73bp
FT                   HaeII = RGCGC^Y"
FT   -               74..295
FT                   /note="pBR322 2352..2573 222bp
FT                   TaqI = T^CGA"
FT   -               296..1739
FT                   /note="pUC8 998..2441 1444bp
FT                   TaqI = T^CGA"
FT   -               1740..2010
FT                   /note="pBR322 4018..4288 271bp
FT                   AatII = GACGT^C
FT                   RsaI =     GT^AC"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2010 BP; 532 A; 496 C; 503 G; 479 T; 0 other;
     gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc
     gtgctgctag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgaac
     tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     cacatttccc cgaaaagtgc cacctgacgt
//