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ID   PNEB193    preliminary; circular DNA; SYN; 2713 BP.
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AC   IG2000; X02514; X02513;
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DT   01-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pNEB193 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pNEB193 from pUC19
RA   Guan C.D.;
RT   ;
RL   Unpublished (1993).
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CC   NM (pNEB193)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (NEB)
CC   HO (E.coli JM103)(E.coli DH5alpha)(E.coli DH10B)(E.coli TB1)
CC   HO (E.coli JM83)(E.coli JM105)(E.coli JM109)(E.coli NM522)
CC   CP (high)
CC   FN (cloning general)(sequencing dideoxy)
CC   SE (color blue/white)
CC   PA (pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp
FT                   -> pNEB193 2713bp"
FT   misc_binding    179..179
FT                   /note="SIT unique ApaBI"
FT   misc_binding    183..183
FT                   /note="SIT unique NdeI"
FT   misc_binding    235..235
FT                   /note="SIT unique NarI"
FT   CDS             complement(236..469)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    396..479
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-AscI-BamHI-PacI-
FT                   XbaI-SalI-PmeI-BspMI-PstI-SphI-HindIII"
FT   misc_binding    710..710
FT                   /note="SIT unique SapI"
FT   misc_binding    833..833
FT                   /note="SIT unique AflIII"
FT   rep_origin      complement(888..1414)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    1244..1244
FT                   /note="SIT unique AlwNI"
FT   CDS             complement(1653..2513)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1721..1721
FT                   /note="SIT unique Eam1105I"
FT   misc_binding    1742..1742
FT                   /note="SIT unique Pfl108I"
FT   misc_binding    1793..1793
FT                   /note="SIT unique BsaI"
FT   misc_binding    1806..1806
FT                   /note="SIT unique BsrFI"
FT   misc_binding    1811..1811
FT                   /note="SIT unique BpmI"
FT   misc_binding    2204..2204
FT                   /note="SIT unique ScaI"
FT   misc_binding    2242..2242
FT                   /note="SIT unique BcgI"
FT   misc_binding    2321..2321
FT                   /note="SIT unique XmnI"
FT   misc_binding    2528..2528
FT                   /note="SIT unique SspI"
FT   misc_binding    2644..2644
FT                   /note="SIT unique AatII"
FT   misc_binding    2701..2701
FT                   /note="SIT unique EcoO109I"
XX
SQ   Sequence 2713 BP; 675 A; 680 C; 692 G; 666 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccgggggc
     gcgccggatc cttaattaag tctagagtcg actgtttaaa cctgcaggca tgcaagcttg
     gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     ctttctcaaa gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     aggccctttc gtc
//