back Return to this vector's summary.
ID   PNEO       preliminary; circular DNA; SYN; 5508 BP.
XX
AC   IG1096; V00618; J01834; U00004; L19385; M83744;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pNEO - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   F' pro lac from F' factor
RC   F' pro lac::Tn5 from F' pro lac & Tn5
RC   pRZ102, pRZ104 from F' pro lac::Tn5 & ColE1
RC   pRZ111, pRZ112 from pRZ102
RC   pRZ135 from pRZ112 & lambda::Tn10(1)
RA   Jorgensen R.A., Rothstein S.J., Reznikoff W.S.;
RT   "A restriction enzyme cleavage map of Tn5 and location of a region
RT   encoding neomycin resistance";
RL   Mol. Gen. Genet. 177:65-72(1979).
XX
RN   [2]
RC   [pNEO from pBR322 & Tn5, neo gene]
RC   pAG40, pAG45 from pAG0 & Tn5
RC   pAG50, pAG55 from pAG40 & pAG0
RC   pAG60 from pAG50
RC   pFG22, pFG24 from pAG0 & HSV ori
RC   pAG70 from pAG60 & pFG24
RA   Colbere-Garapin F., Horodniceanu F., Kourilsky P., Garapin A.C.;
RT   "A new dominant hybrid selective marker for higher eukaryotic
RT   cells";
RL   J. Mol. Biol. 150:1-14(1981).
XX
RN   [3]
RC   pEX205 from pBR322
RA   Reiss B.;
RT   "The neomycin phosphotransferase gene as an indicator for gene
RT   expression signals";
RL   Thesis [Univ. of Heidelberg] 0:0-0(1982).
XX
CC   Excise neomycin with HindIII and BamHI and insert 3' to
CC   eukaryotic promoter.
CC   NM (pNEO)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli)(plant)(mammal)
CC   CP ()
CC   FN (cloning)(marker dominant selectable)
CC   SE ()
CC   PA (pBR322)(Tn5)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   transposition
FT                   2. Tn5 5818bp
FT                   -> pRZ102 10179bp
FT                   1. pRZ102 remove pBR322 sequences
FT                   -> pRZ112
FT                   1. pRZ112 HincII-HincII 2499bp 188..2687, neo gene
FT                   BamHI linker 10bp nnggatccnn:BamHI linker 10bp
FT                   \ nnggatccnn
FT                   HindIII-BamHI 1493bp 1196..2689, neo/kan gene
FT                   2. pBR322 remove HindIII-BamHI 346bp 30..376, 4015bp
FT                   -> pNEO 5508bp"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo),
FT                   kanamycin resistance gene (kan),
FT                   aminoglycoside 3'-phosphotransferase (APH)"
FT   misc_binding    0..0
FT                   /note="SIT unique BclI"
FT   misc_binding    0..0
FT                   /note="SIT unique BglII"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuI"
FT   misc_binding    0..0
FT                   /note="SIT unique ScaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
XX
SQ   Sequence 5508 BP; 1218 A; 1521 C; 1518 G; 1251 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gcttcacgct gccgcaagca ctcagggcgc
     aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc
     cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
     caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
     agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
     aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa
     gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     gggatctcat gctggagttc ttcgcccacc ccgggctcga tcccctcgcg agttggttca
     gctgctgcct gaggctggac gacctcgcgg agttctaccg gcagtgcaaa tccgtcggca
     tccaggaaac cagcagcggc tatccgcgca tccatgcccc cgaactgcag gagtggggag
     gcacgatggc cgctttggtc ccggatcctc tacgccggac gcatcgtggc cggcatcacc
     ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggcccgtgg
     ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
     ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
     catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac
     tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg
     gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag
     cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg
     ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag
     cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg
     tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg
     catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg
     gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc
     ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct
     ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc
     agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt
     aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc
     gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg
     tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaa
//