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ID   PNKY2003   preliminary; circular DNA; SYN; 6603 BP.
XX
AC   IG9899;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector pNKY2003 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pNKY2003 from YEp24
RC   pNKY2005 from pNKY2003 & B54
RC   pNKY2010 from pNKY2005 & YEp13
RC   pNKY2009 from pNKY2010 & YEp24
RC   pNKY2022 from pNKY2009 & linker
RC   pNKY2024 from pNKY2022
RC   B184 from B115 & yeast TD50
RC   pNKY2028 from pNKY2024 & B184
RC   pNKY2029 from pNKY2024 & linker
RC   plasmid from pNKY2029 & YRp7
RC   pNKY54 from plasmid & pBR322
RC   pNKY47 from pGC1 & linker
RC   pNKY48 from pNKY2029 & pNKY47
RC   pNKY59, pNKY60 from pNKY48 & pUR290
RC   pNKY70 from pNKY48 & linker
RC   pNKY71 from pNKY70 & pRY116
RC   pNKY1069 from pNKY71 & YRp7
RA   Alani E., Kleckner N.;
RT   "A new type of fusion analysis applicable to many organisms:
RT   protein fusions to the URA3 gene of yeast";
RL   Genetics 117:5-12(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pNKY2003)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (YEp24)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp24 remove HindIII-HindIII 1166bp
FT                   \ 2273..3439, URA3 gene/6603bp
FT                   -> pNKY2003 6603bp"
FT   -               1..6603
FT                   /note="YEp24 3439..7769..2272 6603bp
FT                   HindIII = A^AGCTT"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
XX
SQ   Sequence 6603 BP; 1720 A; 1644 C; 1574 G; 1665 T; 0 other;
     agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga
     aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag
     gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg
     ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg
     ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg
     gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg
     gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
     tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
     cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
     aggagtcgca taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct
     ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
     gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
     ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg
     ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga
     tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg
     ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg
     ctcttaccag cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct
     cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc
     tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg
     gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac
     tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag
     ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct
     cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc
     accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac
     aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc
     ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc
     tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg
     catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc
     agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag
     aaattccccc ttacacggag gcatcaagtg accaaacagg aaaaaaccgc ccttaacatg
     gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg
     gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc
     tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     ttcgtcttca agaattctga accagtccta aaacgagtaa ataggaccgg caattcttca
     agcaataaac aggaatacca attattaaaa gataacttag tcagatcgta caataaagct
     ttgaagaaaa atgcgcctta ttcaatcttt gctataaaaa atggcccaaa atctcacatt
     ggaagacatt tgatgacctc atttctttca atgaagggcc taacggagtt gactaatgtt
     gtgggaaatt ggagcgataa gcgtgcttct gccgtggcca ggacaacgta tactcatcag
     ataacagcaa tacctgatca ctacttcgca ctagtttctc ggtactatgc atatgatcca
     atatcaaagg aaatgatagc attgaaggat gagactaatc caattgagga gtggcagcat
     atagaacagc taaagggtag tgctgaagga agcatacgat accccgcatg gaatgggata
     atatcacagg aggtactaga ctacctttca tcctacataa atagacgcat ataagtacgc
     atttaagcat aaacacgcac tatgccgttc ttctcatgta tatatatata caggcaacac
     gcagatatag gtgcgacgtg aacagtgagc tgtatgtgcg cagctcgcgt tgcattttcg
     gaagcgctcg ttttcggaaa cgctttgaag ttcctattcc gaagttccta ttctctagaa
     agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc tctgaagacg cactttcaaa
     aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata ttgcgaatac cgcttccaca
     aacattgctc aaaagtatct ctttgctata tatctctgtg ctatatccct atataaccta
     cccatccacc tttcgctcct tgaacttgca tctaaactcg acctctacat tttttatgtt
     tatctctagt attactcttt agacaaaaaa attgtagtaa gaactattca tagagtgaat
     cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt atatagagac aaaatagaag
     aaaccgttca taattttctg accaatgaag aatcatcaac gctatcactt tctgttcaca
     aagtatgcgc aatccacatc ggtatagaat ataatcgggg atgcctttat cttgaaaaaa
     tgcacccgca gcttcgctag taatcagtaa acgcgggaag tggagtcagg ctttttttat
     ggaagagaaa atagacacca aagtagcctt cttctaacct taacggacct acagtgcaaa
     aagttatcaa gagactgcat tatagagcgc acaaaggaga aaaaaagtaa tctaagatgc
     tttgttagaa aaatagcgct ctcgggatgc atttttgtag aacaaaaaag aagtatagat
     tctttgttgg taaaatagcg ctctcgcgtt gcatttctgt tctgtaaaaa tgcagctcag
     attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttttacaa aaatgaagca
     cagattcttc gttggtaaaa tagcgctttc gcgttgcatt tctgttctgt aaaaatgcag
     ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttc tacaaaatga
     agcacagatg cttcgttaac aaagatatgc tattgaagtg caagatggaa acgcagaaaa
     tgaaccgggg atgcgacgtg caagattacc tatgcaatag atgcaatagt ttctccagga
     accgaaatac atacattgtc ttccgtaaag cgctagacta tatattatta tacaggttca
     aatatactat ctgtttcagg gaaaactccc aggttcggat gttcaaaatt caatgatggg
     taacaagtac gatcgtaaat ctgtaaaaca gtttgtcgga tattaggctg tatctcctca
     aagcgtattc gaatatcatt gagaagctgc agcgtcacat cggataataa tgatggcagc
     cattgtagaa gtgccttttg catttctagt ctctttctcg gtctagctag ttttactaca
     tcgcgaagat agaatcttag atcacactgc ctttgctgag ctggatcaat agagtaacaa
     aagagtggta aggcctcgtt aaaggacaag gacctgagcg gaagtgtatc gtacagtaga
     cggagtatac tagtatagtc tatagtccgt ggaattctca tgtttgacag cttatcatcg
     ata
//