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ID   PNKY3      preliminary; circular DNA; SYN; 4088 BP.
XX
AC   IG9970;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector pNKY3 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pRB91 from Salmonella hisOGD region
RC   pNK294 from pRB91, Salmonella hisOGD region
RC   pNKY3 from YEp24 & linker
RC   pNKY49 from pNK294 & pNKY3
RC   pNKY50 from pNKY49 & pNK294
RC   pNKY51 from pNKY50
RC   pNKY1006 from YRp7 & linker
RC   pNKY1009 from pNKY1006 & pNKY51
RA   Alani E., Cao L., Kleckner N.;
RT   "A method for gene disruption that allows repeated use of URA3
RT   selection in the construction of multiply disrupted yeast
RT   strains";
RL   Genetics 116:541-545(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pNKY3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (YEp24)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp24 remove EcoRI-EcoRI 2240bp 2..2242,
FT                   \ yeast 2 micron ori/4078bp
FT                   BglII linker 6bp agatct
FT                   -> pNKY3 5530bp [unique EcoRI]"
FT   -               1..5
FT                   /note="YEp24 1..5 5bp
FT                   EcoRI = G^AATT C
FT                   \              agatct"
FT   -               6..11
FT                   /note="agatct 6bp
FT                   \  agatct
FT                   EcoRI = G^AATTC"
FT   -               12..4088
FT                   /note="YEp24 2242..6318 4077bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
XX
SQ   Sequence 4088 BP; 976 A; 1064 C; 1075 G; 973 T; 0 other;
     gaattagatc taattctcat gtttgacagc ttatcatcga taagcttttc aattcaattc
     atcatttttt ttttattctt ttttttgatt tcggtttctt tgaaattttt ttgattcggt
     aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg tatatatacg
     catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac tgcacagaac
     aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg aacgtgctgc
     tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa agcaaacaaa
     cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag ttgaagcatt
     aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt tttccatgga
     gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac tcttcgaaga
     cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg gtgtatacag
     aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag gtattgttag
     cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt tgatgttagc
     agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta ctgttgacat
     tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca tgggtggaag
     agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg acaagggaga
     cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat ctgacattat
     tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta
     cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact aaaaaactgt
     attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt aattatatca
     gttattaccc gggaatctcg gtcgtaatga tttttataat gacgaaaaaa aaaaaattgg
     aaagaaaaag ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc
     gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt
     aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga
     cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg
     cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc
     gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcct
     ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag ccttcaaccc
     agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
     ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
     cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
     cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
     aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
     gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc
     gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta
     tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct
     tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga
     agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg
     cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc
     tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg
     atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag
     aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc
     tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag
     tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt
     ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg
     tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt
     tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc
     atgaacagaa attccccctt acacggaggc atcaagtgac caaacaggaa aaaaccgccc
     ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc
     tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt
     accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     gattagca
//