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ID PNN414 preliminary; circular DNA; SYN; 15809 BP.
XX
AC ATCC37682;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Saccharomyces/vertebrate/E.coli YAC vector pNN414 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pYACneo from pYAC4 & pSV2neo
RC hX1 from pYACneo & human genome
RC pNN415 from hX1
RA Traver C.N., Klapholz S., Hyman R.W., Davis R.W.;
RT "Rapid screening of a human genomic library in yeast artificial
RT chromosomes for single-copy sequences";
RL Proc. Natl. Acad. Sci. U.S.A. 86:5898-5902(1989).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC Restriction digests of the clone give the following sizes (kb):
CC EcoRI--15.9; XhoI--11.0, 3.4, 1.0; (ATCC staff)
CC BamHI--14.0, 1.85; BamHI/EcoRI--8.2, 5.8, 1.85; SalI/BamHI--7.4, 6.6,
CC 1.85. (ATCC staff)
CC XhoI linkers were ligated to the blunt ended 4.2 kb PvuI/BamHI
CC fragment of pSV2neo (with neomycin resistance and ColE1 origin). This
CC was then cloned into the SalI site of pYAC4. Contains telomer
CC elements from Tetrahymena.
CC To facilitate chromosome walking, the ends of the insert can be
CC recovered in E.coli. The artificial chromosomal DNA can be digested
CC with XhoI, ligated, and used to transform E.coli to amp or kan
CC resistance.
CC Cloning into the EcoRI site insertionally inactivates SUP4.
CC G418 resistance is expressed in mammalian cell lines to permit genetic
CC complementation experiments. Constructed by Christopher Traver.
CC Medium is 1227 LB plus ampicillin.
CC NM (pNN414)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (yeast artificial chromosome)
CC SP (ATCC)
CC HO (E.coli DH1)(Saccharomyces cerevisiae)(vertebrate cells)(E.coli)
CC HO (Saccharomyces cerevisiae AB1380)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pSV2-neo)(pYAC4)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pSV2-neo BamHI-PvuI 4355bp 752..5107,
FT \ ori/neo gene
FT blunt end:blunt end
FT XhoI linker 6bp ctcgag:XhoI linker 6bp ctcgag
FT XhoI-XhoI 4360bp, ori/neo gene
FT 2. pYAC4 SalI 11454bp 944..944
FT -> pNN414 15700bp [EcoRI in SUP4]"
FT - 1..943
FT /note="pYAC4 1..943 943bp
FT SalI = G^TCGAC
FT XhoI = C^TCGAG
FT \ tcgag"
FT - 944..948
FT /note="tcgag 5bp
FT \ tcgag
FT XhoI = C^TCGAG
FT BamHI = G^GATC C
FT - 949..5297
FT /note="pSV2-neo 756..5104 4349bp
FT PvuI = CG AT^CG
FT XhoI = C^TCGAG
FT \ c"
FT - 5298..5298
FT /note="c 1bp
FT \ c
FT XhoI = C^TCGAG
FT SalI = G^TCGAC"
FT - 5299..15809
FT /note="pYAC4 944..11454 10511bp"
FT misc_binding 0..0
FT /note="SIT unique EcoRI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 0..0
FT /note="ORI yeast ARS1"
FT misc_feature 0..0
FT /note="yeast centromere CEN4"
FT promoter 0..0
FT /note="PRO yeast SUP4 gene"
FT CDS 0..0
FT /note="ANT E. coli neomycin phosphotransferase gene
FT (NPT); neomycin resistance gene (neo)"
FT CDS 0..0
FT /note="ANT yeast TRP1 gene"
FT CDS 0..0
FT /note="ANT yeast HIS3 gene"
FT CDS 0..0
FT /note="ANT yeast URA3 gene"
FT CDS 0..0
FT /note="ANT yeast SUP4 gene (ochre suppressor)"
XX
SQ Sequence 15809 BP; 4215 A; 3690 C; 3668 G; 4236 T; 0 other;
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
cacacccgtc ctgtggatca attcccttta gtataaattt cactctgaac catcttggaa
ggaccggtaa ttatttcaaa tctctttttc aattgtatat gtgttatgtt atgtagtata
ctctttcttc aacaattaaa tactctcggt agccaagttg gtttaaggcg caagacttta
atttatcact acggaattcc gtaatcttga gatcgggcgt tcgatcgccc cgggagattt
ttttgttttt tatgtcttcc attcacttcc cagacttgca agttgaaata tttctttcaa
gggaattgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagca gacatgataa
gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctctag tcaaggcact
atacatcaaa tattccttat taaccccttt acaaattaaa aagctaaagg tacacaattt
ttgagcatag ttattaatag cagacactct atgcctgtgt ggagtaagaa aaaacagtat
gttatgatta taactgttat gcctacttat aaaggttaca gaatattttt ccataatttt
cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag caagagttct
attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt ccttggggtc
ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag tagcctcatc
atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg cattccacca
ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa ttagaatcag
tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt aaatctctgt
aggtagtttg tccaattatg tcacaccaca gaagtaaggt tccttcacaa agatccggga
ccaaagcggc catcgtgcct ccccactcct gcagttcggg ggcatggatg cgcggatagc
cgctgctggt ttcctggatg ccgacggatt tgcactgccg gtagaactcc gcgaggtcgt
ccagcctcag gcagcagctg aaccaactcg cgaggggatc gagcccgggg tgggcgaaga
actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc
cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg
gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag
gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc
attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag
cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca
gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg
cttcctttag cagcccttgc gccctgagtg cttgcggcag cgtgaagctt tttgcaaaag
cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
ccacacccta actgacacac attccacagc tgcctcgcgc gtttcggtga tgacggtgaa
aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
gctccttcgg tcctccgctc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg
gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca
agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg
catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc
cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat
gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct
taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc
gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc
cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag ccggcggcac
ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg gagaactgtg
aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc cagcagccgc
acgcggcgca tccccccccc cctttcaatt caattcatca tttttttttt attctttttt
ttgatttcgg tttctttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg
aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg
aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga aacgaagata
aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc
caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg
taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa
aacacatgtg gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc
attatccgcc aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa
tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac
gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga
agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct
atctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt
tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat
tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac
cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag gactatttgc
aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata
tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact
aaactcacaa attagagctt caatttaatt atatcagtta ttactcgggc gtaatgattt
ttataatgac gaaaaaaaaa aaattggaaa gaaaaggggg ggggggcagc gttgggtcct
ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt
gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct
gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa
gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat
gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct
gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt
ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca
tcggtatcat tacccccatg aacagaaatt cccccttaca cggaggcatc aagtgaccaa
acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat taacgcttct
ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga
ccacgctgat gagctttacc gcagccctcg agggataagc ttcattttta gataaaattt
attaatcatc attaatttct tgaaaaacat tttatttatt gatcttttat aacaaaaaac
ccttctaaaa gtttattttt gaatgaaaaa cttataaaaa tttatgaaaa ctacaaaaaa
taaaattttt aattaaaata attttgataa gaacttcaat ctttgactag ctagcttagt
catttttgag atttaattaa tattttatgt ttattcatat ataaactatt caaaatatta
tagaatttaa acattttaac atcttaatca ttcataaata actaaaaatc aaagtattac
atcaataaat aacttttact caatgtcaaa gaattattgg ggttggggtt ggggttgggg
ttggggttgg ggttggggtt ggggttgggg ttggggttgg ggttggggtt ggggttgggg
ttggggttgg ggttggggtt ggggttgggg ttggggttgg ggttggggtt ggggttgggg
ttggggttgg ggttggggtt ggggttgggg ttggggttgg ggttggggtt ggggttgggg
ttggggttgg ggttggggtt ggggttgggg ttggggttgg ggttggggtt ggggttgggg
ttggggtggg aaaacagcat tcaggtatta gaagaatatc ctgattcagg tgaaaatatt
gttgatgcgc gggatcctcg gggacaccaa atatggcgat ctcggccttt tcgtttcttg
gagctgggac atgtttgcca tcgatccatc taccaccaga acggccgtta gatctgctgc
caccgttgtt tccaccgaag aaaccaccgt tgccgtaacc accacgacgg ttgttgctaa
agaagctgcc accgccacgg ccaccgttgt agccgccgtt gttgttattg tagttgctca
tgttatttct ggcacttctt ggttttcctc ttaagtgagg aggaacataa ccattctcgt
tgttgtcgtt gatgcttaaa ttttgcactt gttcgctcag ttcagccata atatgaaatg
cttttcttgt tgttcttacg gaataccact tgccacctat caccacaact aactttttcc
cgttcctcca tctcttttat attttttttc tcgatcgagt tcaagagaaa aaaaaagaaa
aagcaaaaag aaaaaaggaa agcgcgcctc gttcagaatg acacgtatag aatgatgcat
taccttgtca tcttcagtat catactgttc gtatacatac ttactgacat tcataggtat
acatatatac acatgtatat atatcgtatg ctgcagcttt aaataatcgg tgtcactaca
taagaacacc tttggtggag ggaacatcgt tggtaccatt gggcgaggtg gcttctctta
tggcaaccgc aagagccttg aacgcactct cactacggtg atgatcattc ttgcctcgca
gacaatcaac gtggagggta attctgctag cctctgcaaa gctttcaaga aaatgcggga
tcatctcgca agagagatct cctactttct ccctttgcaa accaagttcg acaactgcgt
acggcctgtt cgaaagatct accaccgctc tggaaagtgc ctcatccaaa ggcgcaaatc
ctgatccaaa cctttttact ccacgcgcca gtagggcctc tttaaaagct tgaccgagag
caatcccgca gtcttcagtg gtgtgatggt cgtctatgtg taagtcacca atgcactcaa
cgattagcga ccagccggaa tgcttggcca gagcatgtat catatggtcc agaaacccta
tacctgtgtg gacgttaatc acttgcgatt gtgtggcctg ttctgctact gcttctgcct
ctttttctgg gaagatcgag tgctctatcg ctaggggacc accctttaaa gagatcgcaa
tctgaatctt ggtttcattt gtaatacgct ttactagggc tttctgctct gtcatctttg
ccttcgttta tcttgcctgc tcatttttta gtatattctt cgaagaaatc acattacttt
atataatgta taattcatta tgtgataatg ccaatcgcta agaaaaaaaa agagtcatcc
gctaggtgga aaaaaaaaaa tgaaaatcat taccgaggca taaaaaaata tagagtgtac
tagaggaggc caagagtaat agaaaaagaa aattgcggga aaggactgtg ttatgacttc
cctgactaat gccgtgttca aacgatacct ggcagtgact cctagcgctc accaagctct
taaaacgaga attaagaaaa agtcgtcatc tttcgataag tttttcccac agcaaagcaa
tagtagaaaa aaacaatggg aaacgttgaa tgaagacaaa gcgtcgtggt ttaaaaggaa
atacgctcac gtacatgcta gggaacagga ccgtgcagcg gatcccgcgc atcaacaata
ttttcacctg aatcaggata ttcttctaat acctgaatgc tgttttccca ccccaacccc
aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa ccccaacccc
aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa ccccaacccc
aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa ccccaacccc
aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa ccccaacccc
aaccccaacc ccaaccccaa taattctttg acattgagta aaagttattt attgatgtaa
tactttgatt tttagttatt tatgaatgat taagatgtta aaatgtttaa attctataat
attttgaata gtttatatat gaataaacat aaaatattaa ttaaatctca aaaatgacta
agctagctag tcaaagattg aagttcttat caaaattatt ttaattaaaa attttatttt
ttgtagtttt cataaatttt tataagtttt tcattcaaaa ataaactttt agaagggttt
tttgttataa aagatcaata aataaaatgt ttttcaagaa attaatgatg attaataaat
tttatctaaa aatgaagctt atccctcgag ggctgcctcg cgcgtttcgg tgatgacggt
gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatta attcggtcga
aaaaagaaaa ggagagggcc aagagggagg gcattggtga ctattgagca cgtgagtata
cgtgattaag cacacaaagg cagcttggag tatgtctgtt attaatttca caggtagttc
tggtccattg gtgaaagttt gcggcttgca gagcacagag gccgcagaat gtgctctaga
ttccgatgct gacttgctgg gtattatatg tgtgcccaat agaaagagaa caattgaccc
ggttattgca aggaaaattt caagtcttgt aaaagcatat aaaaatagtt caggcactcc
gaaatacttg gttggcgtgt ttcgtaatca acctaaggag gatgttttgg ctctggtcaa
tgattacggc attgatatcg tccaactgca tggagatgag tcgtggcaag aataccaaga
gttcctcggt ttgccagtta ttaaaagact cgtatttcca aaagactgca acatactact
cagtgcagct tcacagaaac ctcattcgtt tattcccttg tttgattcag aagcaggtgg
gacaggtgaa cttttggatt ggaactcgat ttctgactgg gttggaaggc aagagagccc
cgaaagctta cattttatgt tagctggtgg actgacgcca gaaaatgttg gtgatgcgct
tagattaaat ggcgttattg gtgttgatgt aagcggaggt gtggagacaa atggtgtaaa
agactctaac aaaatagcaa atttcgtcaa aaatgctaag aaataggtta ttactgagta
gtatttattt aagtattgtt tgtgcacttg cctgcaggcc ttttgaaaag caagcataaa
agatctaaac ataaaatctg taaaataaca agatgtaaag ataatgctaa atcatttggc
tttttgattg attgtacagg aaaatataca tcgcaggggg ttgactttta ccatttcacc
gcaatggaat caaacttgtt gaagagaatg ttcacaggcg catacgctac aatgacccga
ttcttgctag ccttttctcg gtcttgcaaa caaccgccgg cagcttagta tataaataca
catgtacata cctctctccg tatcctcgta atcattttct tgtatttatc gtcttttcgc
tgtaaaaact ttatcacact tatctcaaat acacttatta accgctttta ctattatctt
ctacgctgac agtaatatca aacagtgaca catattaaac acagtggttt ctttgcataa
acaccatcag cctcaagtcg tcaagtaaag atttcgtgtt catgcagata gataacaatc
tatatgttga taattagcgt tgcctcatca atgcgagatc cgtttaaccg gaccctagtg
cacttacccc acgttcggtc cactgtgtgc cgaacatgct ccttcactat tttaacatgt
ggaattaatt ctaaatcctc tttatatgat ctgccgatag atagttctaa gtcattgagg
ttcatcaaca attggatttt ctgtttactc gacttcaggt aaatgaaatg agatgatact
tgcttatctc atagttaact ctaagaggtg atacttattt actgtaaaac tgtgacgata
aaaccggaag gaagaataag aaaactcgaa ctgatctata atgcctattt tctgtaaaga
gtttaagcta tgaaagcctc ggcattttgg ccgctcctag gtagtgcttt ttttccaagg
acaaaacagt ttctttttct tgagcaggtt ttatgtttcg gtaatcataa acaataaata
aattatttca tttatgttta aaaataaaaa ataaaaaagt attttaaatt tttaaaaaag
ttgattataa gcatgtgacc ttttgcaagc aattaaattt tgcaatttgt gattttaggc
aaaagttaca atttctggct cgtgtaatat atgtatgcta aagtgaactt ttacaaagtc
gatatggact tagtcaaaag aaattttctt aaaaatatat agcactagcc aatttagcac
ttctttatga gatatattat agactttatt aagccagatt tgtgtattat atgtatttac
ccggcgaatc atggacatac attctgaaat aggtaatatt ctctatggtg agacagcata
gataacctag gatacaagtt aaaagctagt actgttttgc agtaattttt ttctttttta
taagaatgtt accacctaaa taagttataa agtcaatagt taagtttgat atttgattgt
aaaataccgt aatatatttg catgatcaaa aggctcaatg ttgactagcc agcatgtcaa
ccactatatt gatcaccgat atatggactt ccacaccaac tagtaatatg acaataaatt
caagatattc ttcatgagaa tggcccaga
//