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ID   PNN414     preliminary; circular DNA; SYN; 15809 BP.
XX
AC   ATCC37682;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/vertebrate/E.coli YAC vector pNN414 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pYACneo from pYAC4 & pSV2neo
RC   hX1 from pYACneo & human genome
RC   pNN415 from hX1
RA   Traver C.N., Klapholz S., Hyman R.W., Davis R.W.;
RT   "Rapid screening of a human genomic library in yeast artificial
RT   chromosomes for single-copy sequences";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:5898-5902(1989).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--15.9; XhoI--11.0, 3.4, 1.0; (ATCC staff)
CC   BamHI--14.0, 1.85; BamHI/EcoRI--8.2, 5.8, 1.85; SalI/BamHI--7.4, 6.6,
CC   1.85. (ATCC staff)
CC   XhoI linkers were ligated to the blunt ended 4.2 kb PvuI/BamHI
CC   fragment of pSV2neo (with neomycin resistance and ColE1 origin). This
CC   was then cloned into the SalI site of pYAC4. Contains telomer
CC   elements from Tetrahymena.
CC   To facilitate chromosome walking, the ends of the insert can be
CC   recovered in E.coli. The artificial chromosomal DNA can be digested
CC   with XhoI, ligated, and used to transform E.coli to amp or kan
CC   resistance.
CC   Cloning into the EcoRI site insertionally inactivates SUP4.
CC   G418 resistance is expressed in mammalian cell lines to permit genetic
CC   complementation experiments. Constructed by Christopher Traver.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pNN414)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (yeast artificial chromosome)
CC   SP (ATCC)
CC   HO (E.coli DH1)(Saccharomyces cerevisiae)(vertebrate cells)(E.coli)
CC   HO (Saccharomyces cerevisiae AB1380)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pSV2-neo)(pYAC4)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSV2-neo BamHI-PvuI 4355bp 752..5107,
FT                   \ ori/neo gene
FT                   blunt end:blunt end
FT                   XhoI linker 6bp ctcgag:XhoI linker 6bp ctcgag
FT                   XhoI-XhoI 4360bp, ori/neo gene
FT                   2. pYAC4 SalI 11454bp 944..944
FT                   -> pNN414 15700bp [EcoRI in SUP4]"
FT   -               1..943
FT                   /note="pYAC4 1..943 943bp
FT                   SalI = G^TCGAC
FT                   XhoI = C^TCGAG
FT                   \        tcgag"
FT   -               944..948
FT                   /note="tcgag 5bp
FT                   \         tcgag
FT                   XhoI =  C^TCGAG
FT                   BamHI =  G^GATC C
FT   -               949..5297
FT                   /note="pSV2-neo 756..5104 4349bp
FT                   PvuI =  CG AT^CG
FT                   XhoI =     C^TCGAG
FT                   \          c"
FT   -               5298..5298
FT                   /note="c 1bp
FT                   \      c
FT                   XhoI = C^TCGAG
FT                   SalI = G^TCGAC"
FT   -               5299..15809
FT                   /note="pYAC4 944..11454 10511bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN4"
FT   promoter        0..0
FT                   /note="PRO yeast SUP4 gene"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   CDS             0..0
FT                   /note="ANT yeast HIS3 gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   CDS             0..0
FT                   /note="ANT yeast SUP4 gene (ochre suppressor)"
XX
SQ   Sequence 15809 BP; 4215 A; 3690 C; 3668 G; 4236 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatca attcccttta gtataaattt cactctgaac catcttggaa
     ggaccggtaa ttatttcaaa tctctttttc aattgtatat gtgttatgtt atgtagtata
     ctctttcttc aacaattaaa tactctcggt agccaagttg gtttaaggcg caagacttta
     atttatcact acggaattcc gtaatcttga gatcgggcgt tcgatcgccc cgggagattt
     ttttgttttt tatgtcttcc attcacttcc cagacttgca agttgaaata tttctttcaa
     gggaattgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagca gacatgataa
     gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctctag tcaaggcact
     atacatcaaa tattccttat taaccccttt acaaattaaa aagctaaagg tacacaattt
     ttgagcatag ttattaatag cagacactct atgcctgtgt ggagtaagaa aaaacagtat
     gttatgatta taactgttat gcctacttat aaaggttaca gaatattttt ccataatttt
     cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag caagagttct
     attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt ccttggggtc
     ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag tagcctcatc
     atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg cattccacca
     ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa ttagaatcag
     tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt aaatctctgt
     aggtagtttg tccaattatg tcacaccaca gaagtaaggt tccttcacaa agatccggga
     ccaaagcggc catcgtgcct ccccactcct gcagttcggg ggcatggatg cgcggatagc
     cgctgctggt ttcctggatg ccgacggatt tgcactgccg gtagaactcc gcgaggtcgt
     ccagcctcag gcagcagctg aaccaactcg cgaggggatc gagcccgggg tgggcgaaga
     actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc
     cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg
     gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc
     attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
     caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag
     cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca
     gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg
     cttcctttag cagcccttgc gccctgagtg cttgcggcag cgtgaagctt tttgcaaaag
     cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
     tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     ccacacccta actgacacac attccacagc tgcctcgcgc gtttcggtga tgacggtgaa
     aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     gctccttcgg tcctccgctc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg
     gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca
     agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg
     catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc
     cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat
     gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct
     taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc
     gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc
     cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag ccggcggcac
     ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg gagaactgtg
     aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc cagcagccgc
     acgcggcgca tccccccccc cctttcaatt caattcatca tttttttttt attctttttt
     ttgatttcgg tttctttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg
     aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg
     aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga aacgaagata
     aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc
     caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg
     taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa
     aacacatgtg gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc
     attatccgcc aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa
     tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac
     gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga
     agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct
     atctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt
     tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat
     tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac
     cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag gactatttgc
     aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata
     tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact
     aaactcacaa attagagctt caatttaatt atatcagtta ttactcgggc gtaatgattt
     ttataatgac gaaaaaaaaa aaattggaaa gaaaaggggg ggggggcagc gttgggtcct
     ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt
     gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct
     gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa
     gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat
     gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct
     gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt
     ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca
     tcggtatcat tacccccatg aacagaaatt cccccttaca cggaggcatc aagtgaccaa
     acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat taacgcttct
     ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga
     ccacgctgat gagctttacc gcagccctcg agggataagc ttcattttta gataaaattt
     attaatcatc attaatttct tgaaaaacat tttatttatt gatcttttat aacaaaaaac
     ccttctaaaa gtttattttt gaatgaaaaa cttataaaaa tttatgaaaa ctacaaaaaa
     taaaattttt aattaaaata attttgataa gaacttcaat ctttgactag ctagcttagt
     catttttgag atttaattaa tattttatgt ttattcatat ataaactatt caaaatatta
     tagaatttaa acattttaac atcttaatca ttcataaata actaaaaatc aaagtattac
     atcaataaat aacttttact caatgtcaaa gaattattgg ggttggggtt ggggttgggg
     ttggggttgg ggttggggtt ggggttgggg ttggggttgg ggttggggtt ggggttgggg
     ttggggttgg ggttggggtt ggggttgggg ttggggttgg ggttggggtt ggggttgggg
     ttggggttgg ggttggggtt ggggttgggg ttggggttgg ggttggggtt ggggttgggg
     ttggggttgg ggttggggtt ggggttgggg ttggggttgg ggttggggtt ggggttgggg
     ttggggtggg aaaacagcat tcaggtatta gaagaatatc ctgattcagg tgaaaatatt
     gttgatgcgc gggatcctcg gggacaccaa atatggcgat ctcggccttt tcgtttcttg
     gagctgggac atgtttgcca tcgatccatc taccaccaga acggccgtta gatctgctgc
     caccgttgtt tccaccgaag aaaccaccgt tgccgtaacc accacgacgg ttgttgctaa
     agaagctgcc accgccacgg ccaccgttgt agccgccgtt gttgttattg tagttgctca
     tgttatttct ggcacttctt ggttttcctc ttaagtgagg aggaacataa ccattctcgt
     tgttgtcgtt gatgcttaaa ttttgcactt gttcgctcag ttcagccata atatgaaatg
     cttttcttgt tgttcttacg gaataccact tgccacctat caccacaact aactttttcc
     cgttcctcca tctcttttat attttttttc tcgatcgagt tcaagagaaa aaaaaagaaa
     aagcaaaaag aaaaaaggaa agcgcgcctc gttcagaatg acacgtatag aatgatgcat
     taccttgtca tcttcagtat catactgttc gtatacatac ttactgacat tcataggtat
     acatatatac acatgtatat atatcgtatg ctgcagcttt aaataatcgg tgtcactaca
     taagaacacc tttggtggag ggaacatcgt tggtaccatt gggcgaggtg gcttctctta
     tggcaaccgc aagagccttg aacgcactct cactacggtg atgatcattc ttgcctcgca
     gacaatcaac gtggagggta attctgctag cctctgcaaa gctttcaaga aaatgcggga
     tcatctcgca agagagatct cctactttct ccctttgcaa accaagttcg acaactgcgt
     acggcctgtt cgaaagatct accaccgctc tggaaagtgc ctcatccaaa ggcgcaaatc
     ctgatccaaa cctttttact ccacgcgcca gtagggcctc tttaaaagct tgaccgagag
     caatcccgca gtcttcagtg gtgtgatggt cgtctatgtg taagtcacca atgcactcaa
     cgattagcga ccagccggaa tgcttggcca gagcatgtat catatggtcc agaaacccta
     tacctgtgtg gacgttaatc acttgcgatt gtgtggcctg ttctgctact gcttctgcct
     ctttttctgg gaagatcgag tgctctatcg ctaggggacc accctttaaa gagatcgcaa
     tctgaatctt ggtttcattt gtaatacgct ttactagggc tttctgctct gtcatctttg
     ccttcgttta tcttgcctgc tcatttttta gtatattctt cgaagaaatc acattacttt
     atataatgta taattcatta tgtgataatg ccaatcgcta agaaaaaaaa agagtcatcc
     gctaggtgga aaaaaaaaaa tgaaaatcat taccgaggca taaaaaaata tagagtgtac
     tagaggaggc caagagtaat agaaaaagaa aattgcggga aaggactgtg ttatgacttc
     cctgactaat gccgtgttca aacgatacct ggcagtgact cctagcgctc accaagctct
     taaaacgaga attaagaaaa agtcgtcatc tttcgataag tttttcccac agcaaagcaa
     tagtagaaaa aaacaatggg aaacgttgaa tgaagacaaa gcgtcgtggt ttaaaaggaa
     atacgctcac gtacatgcta gggaacagga ccgtgcagcg gatcccgcgc atcaacaata
     ttttcacctg aatcaggata ttcttctaat acctgaatgc tgttttccca ccccaacccc
     aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa ccccaacccc
     aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa ccccaacccc
     aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa ccccaacccc
     aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa ccccaacccc
     aaccccaacc ccaaccccaa taattctttg acattgagta aaagttattt attgatgtaa
     tactttgatt tttagttatt tatgaatgat taagatgtta aaatgtttaa attctataat
     attttgaata gtttatatat gaataaacat aaaatattaa ttaaatctca aaaatgacta
     agctagctag tcaaagattg aagttcttat caaaattatt ttaattaaaa attttatttt
     ttgtagtttt cataaatttt tataagtttt tcattcaaaa ataaactttt agaagggttt
     tttgttataa aagatcaata aataaaatgt ttttcaagaa attaatgatg attaataaat
     tttatctaaa aatgaagctt atccctcgag ggctgcctcg cgcgtttcgg tgatgacggt
     gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatta attcggtcga
     aaaaagaaaa ggagagggcc aagagggagg gcattggtga ctattgagca cgtgagtata
     cgtgattaag cacacaaagg cagcttggag tatgtctgtt attaatttca caggtagttc
     tggtccattg gtgaaagttt gcggcttgca gagcacagag gccgcagaat gtgctctaga
     ttccgatgct gacttgctgg gtattatatg tgtgcccaat agaaagagaa caattgaccc
     ggttattgca aggaaaattt caagtcttgt aaaagcatat aaaaatagtt caggcactcc
     gaaatacttg gttggcgtgt ttcgtaatca acctaaggag gatgttttgg ctctggtcaa
     tgattacggc attgatatcg tccaactgca tggagatgag tcgtggcaag aataccaaga
     gttcctcggt ttgccagtta ttaaaagact cgtatttcca aaagactgca acatactact
     cagtgcagct tcacagaaac ctcattcgtt tattcccttg tttgattcag aagcaggtgg
     gacaggtgaa cttttggatt ggaactcgat ttctgactgg gttggaaggc aagagagccc
     cgaaagctta cattttatgt tagctggtgg actgacgcca gaaaatgttg gtgatgcgct
     tagattaaat ggcgttattg gtgttgatgt aagcggaggt gtggagacaa atggtgtaaa
     agactctaac aaaatagcaa atttcgtcaa aaatgctaag aaataggtta ttactgagta
     gtatttattt aagtattgtt tgtgcacttg cctgcaggcc ttttgaaaag caagcataaa
     agatctaaac ataaaatctg taaaataaca agatgtaaag ataatgctaa atcatttggc
     tttttgattg attgtacagg aaaatataca tcgcaggggg ttgactttta ccatttcacc
     gcaatggaat caaacttgtt gaagagaatg ttcacaggcg catacgctac aatgacccga
     ttcttgctag ccttttctcg gtcttgcaaa caaccgccgg cagcttagta tataaataca
     catgtacata cctctctccg tatcctcgta atcattttct tgtatttatc gtcttttcgc
     tgtaaaaact ttatcacact tatctcaaat acacttatta accgctttta ctattatctt
     ctacgctgac agtaatatca aacagtgaca catattaaac acagtggttt ctttgcataa
     acaccatcag cctcaagtcg tcaagtaaag atttcgtgtt catgcagata gataacaatc
     tatatgttga taattagcgt tgcctcatca atgcgagatc cgtttaaccg gaccctagtg
     cacttacccc acgttcggtc cactgtgtgc cgaacatgct ccttcactat tttaacatgt
     ggaattaatt ctaaatcctc tttatatgat ctgccgatag atagttctaa gtcattgagg
     ttcatcaaca attggatttt ctgtttactc gacttcaggt aaatgaaatg agatgatact
     tgcttatctc atagttaact ctaagaggtg atacttattt actgtaaaac tgtgacgata
     aaaccggaag gaagaataag aaaactcgaa ctgatctata atgcctattt tctgtaaaga
     gtttaagcta tgaaagcctc ggcattttgg ccgctcctag gtagtgcttt ttttccaagg
     acaaaacagt ttctttttct tgagcaggtt ttatgtttcg gtaatcataa acaataaata
     aattatttca tttatgttta aaaataaaaa ataaaaaagt attttaaatt tttaaaaaag
     ttgattataa gcatgtgacc ttttgcaagc aattaaattt tgcaatttgt gattttaggc
     aaaagttaca atttctggct cgtgtaatat atgtatgcta aagtgaactt ttacaaagtc
     gatatggact tagtcaaaag aaattttctt aaaaatatat agcactagcc aatttagcac
     ttctttatga gatatattat agactttatt aagccagatt tgtgtattat atgtatttac
     ccggcgaatc atggacatac attctgaaat aggtaatatt ctctatggtg agacagcata
     gataacctag gatacaagtt aaaagctagt actgttttgc agtaattttt ttctttttta
     taagaatgtt accacctaaa taagttataa agtcaatagt taagtttgat atttgattgt
     aaaataccgt aatatatttg catgatcaaa aggctcaatg ttgactagcc agcatgtcaa
     ccactatatt gatcaccgat atatggactt ccacaccaac tagtaatatg acaataaatt
     caagatattc ttcatgagaa tggcccaga
//