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ID   PNO1523    preliminary; circular DNA; SYN; 4799 BP.
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AC   IG0092; ATCC37095; J01686; V00357; J01688; V00354; M12490; M12432;
AC   M12489;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pNO1523 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pNO1517 from lambda & pBR322
RC   pNO1523 from pNO1517
RA   Dean D.;
RT   "A plasmid cloning vector for the direct selection of strains carrying
RT   recombinant plasmids";
RL   Gene 15:99-102(1981).
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RN   [2]
RC   pNO2005 from lambda fus3 & pVH51
RA   Post L.E., Nomura M.;
RT   "DNA sequences from the str operon of E. coli";
RL   J. Biol. Chem. 255:4660-4666(1980).
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RN   [3]
RC   [lambda spc2delta9 from lambda spc2]
RC   from lambda spc2delta9
RC   from phage Ch3S45i lambda
RA   Post L.E., Arfsten A.E., Davis G.R., Nomura M.;
RT   "DNA sequence of the promoter region for the alpha ribosomal protein
RT   operon in E. coli";
RL   J. Biol. Chem. 255:4653-4659(1980).
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RN   [4]
RC   lambda fus3 from lambda
RA   Post L.E., Arfsten A.E., Reusser F., Nomura M.;
RT   "DNA sequences of promoter regions for the str and spc ribosomal
RT   protein operons in E.coli";
RL   Cell 15:215-229(1978).
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CC   ATCC size is 5200 bp.
CC   old Pharmacia vector.
CC   A vector for positive selection of clones with inserts based on the
CC   dominance of the plasmid coded rpsL+ gene. [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pNO1523)
CC   NM (pNO1523)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli MC1009)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pNO1517)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pNO1517 remove BstEII-SalI, S12
FT                   DNA polymerase I
FT                   -> pNO1523 4799bp [no SalI, no BstEII]"
FT   promoter        complement(518..891)
FT                   /note="PRO E. coli S12 gene"
FT   promoter        complement(894..937)
FT                   /note="PRO E. coli rpsL+ reporter gene"
FT   misc_binding    0..0
FT                   /note="SIT unique SmaI"
FT   misc_binding    0..0
FT                   /note="SIT unique HpaI"
FT   misc_binding    0..0
FT                   /note="SIT unique SstII/SacII"
FT   rep_origin      3043..3043
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(3801..4661)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   promoter        0..0
FT                   /note="PRO E. coli streptomycin resistance gene"
FT   CDS             0..0
FT                   /note="ANT E. coli streptomycin resistance gene (str)"
FT   CDS             0..0
FT                   /note="GEN E. coli rpsL+ reporter gene"
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SQ   Sequence 4799 BP; 1130 A; 1293 C; 1225 G; 1151 T; 0 other;
     agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     ctgtggatcc ggcagaattt tacgctgacc aatgacgcga cgacgtggca tggaaatact
     ccgttgttaa ttcaggattg tccaaaactc tacgagttta gtttgacatt taagttaaaa
     cgtttggcct tacttaacgg agaaccatta agccttagga cgcttcacgc catacttgga
     acgagcctgc ttacggtctt taacgccgga gcagtcaagc gcaccacgta cggtgtggta
     acgaacaccc gggaggtctt taacacgacc gccacggatc aggatcacgg agtgctcctg
     caggttgtga ccttcaccac cgatgtagga agtcacttcg aaaccgttag tcagacgaac
     acggcatact ttacgcagcg cggagttcgg ttttttagga gtggtagtat atacacgagt
     acatacgcca cgtttttgcg ggcatgcttc cagcgcaggc acgttgcttt tcgcaacttt
     gcgagcacgt ggttgcgtac cagctggtta actgttgcca ttaaatagct cctggtttta
     gcttttgctt cgtaaacacg taataaaacg tcctcacaca atatgaggac gccgaatttt
     agggcgatgc cgaaaaggtg tcaagaaata tacaacgatc ccgccatcat caccaggcca
     tctggctggg gtgcttaacc gtaagtctga cgaaatcagt atagtcaatg agaatgatgt
     cgttcgaaat ttgaccagtc aaaccgcggg caataaggtc ttcgttcagg gcatagacct
     taatgggggc attacgcaga ctttcaaggt agcggttact cagctccttc cggtgggcgc
     ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
     acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
     tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
     aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa
     cttcgatcac tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga
     acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc
     gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg
     attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac
     caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat
     ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac
     ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg
     aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt
     cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt
     ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac
     gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag
     ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt
     gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatt cccccttaca
     cggaggcatc aagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga
     agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac
     atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc
     ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//