back Return to this vector's summary.
ID   PNOT19     preliminary; circular DNA; SYN; 2694 BP.
XX
AC   ATCC77278;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pNOT19 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pMOB1 from pHSS21 & pEMR2, oriT
RC   pMOB2 from pMOB1 & pMH1801, sacBR genes
RC   pMOB3 from pMOB2 & pBR322::Cm
RC   pMOB4 from pMOB3
RC   pSP108 from pUCP19 & pMOB3
RC   pSP114 from pUCP19 & pMOB1
RC   pNOT19 from pUC19 & linker
RC   pNOT322 from pBR322 & linker
RC   pNOT322deltaAP from pNOT322
RC   pBR322deltaAP from pBR322
RC   pPS106 from pPS102 & pBR322deltaAP
RC   pPS106::omegaTc1 from pPS106 & pHP45omega-Tc
RC   pPS112 from pPS106::omegaTc1 & pMOB3
RA   Schweizer H.P.;
RT   "Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type
RT   vectors and a family of cassettes containing a portable oriT and
RT   the counter-selectable Bacillus subtilis sacB marker";
RL   Mol. Microbiol. 6:1195-1204(1992).
XX
RN   [2]
RC   pHCm1 from pHC79 & pSF3, Pseudomonas oriT
RC   pEMR2 from pBR322 & pHCm1
RC   pHIS1 from pEMR2 & hisI gene
RC   pHIS9 from pHIS1
RC   pJF4214 from pEMR2 & Pseudomonas algS(On)T
RC   pJF4221 from pEMR2 & Pseudomonas algS(Off)T
RC   pJF104 from pLAFR1 & Pseudomonas algS(Off)T
RA   Flynn J.L., Ohman D.E.;
RT   "Use of a gene replacement cosmid vetcor for cloning alginate
RT   conversion genes from mucoid and nonmucoid Pseudomonas aeruginosa
RT   strains: algS controls expression of algT";
RL   J. Bacteriol. 170:3228-3236(1988).
XX
RN   [3]
RC   pUC19(Xho) from pUC19 & linker
RC   pUC19(Bgl) from pUC19 & linker
RC   pHSS8deltalacZ from pHSS8
RC   pHSS9 from pHSS8 & pHSS8deltalacZ
RC   pHSS19 from pUC19 & pHSS9
RC   pHSS20 from pUC19(Bgl) & pHSS19
RC   pHSS21 from pUC19(Xho) & pHSS19
RC   pUraB(P) from pUC19 & linker & URA3 gene
RC   plasmid from pHSS19 & GAL1-10 promoter
RC   pUCGalUra from pUraB(P) & plasmid
RA   Nickoloff J.A., Reynolds R.J.;
RT   "Subcloning with new ampicillin- and kanmycin-resistant analogs of
RT   pUC19";
RL   Biotechniques 10:469-472(1991).
XX
RN   [4]
RC   pMH1701 from pUM24 & pJQ17
RC   pMH1801 from pUM24 & pJQ18
RA   Hynes M.F., Quandt J., O'Connell M.P., Puhler A.;
RT   "Direct selection for curing and deletion of Rhizobium plasmids
RT   using transposons carrying the Bacillus subtilis sacB gene";
RL   Gene 78:111-120(1989).
XX
RN   [5]
RC   pBR322::Cm from pBR322 & cat gene
RA   Pato M.;
RT   ;
RL   Unpublished (1992).
XX
RN   [6]
RC   pR#1, pR#2, pR#3, pR#4, pR#5 from phage D3112 & P.aeruginosa chromo
RC   pPS100 from pUCP19 & pR#1
RC   pPS101 from pUCP19 & pR#1
RC   pPS102 from pUCP19 & pR#5, Pseudomonas agmR gene
RC   pPS103 from pUCP19 & pR#1
RC   pEVS2.2 from pPS102 & pUCP18
RC   pSph series from pPS102
RC   pdeltaExo series from pPS102
RC   pUC12-2.2 from pUC12 & pPS102
RC   pGZ1 from pPZ1 & pUC12-2.2
RC   pGZ2 from pPZ2 & pUC12-2.2
RC   pGZ3 from pPZ3 & pUC12-2.2
RC   pT7-5(2.2) from pT7-5 & pEVS2.2
RC   pT7-6(2.2) from pT7-6 & pEVS2.2
RA   Schweizer H.P.;
RT   "The agmR gene, an environmentally responsive gene, complements
RT   defective glpR, encoding the putative activator for glycerol
RT   metabolism in Pseudomonas aeruginosa";
RL   J. Bacteriol. 173:6798-6806(1991).
XX
RN   [7]
RC   pJF15 from pLAFR1 & Pseudomonas algS(On)T
RC   pJF20 from pJF15
RA   Flynn J.L., Ohman D.E.;
RT   "Cloning of genes from mucoid Pseudomonas aeruginosa which control
RT   spontaneous conversion to the alginate production phenotype";
RL   J. Bacteriol. 170:1452-1460(1988).
XX
RN   [8]
RC   pSF3 from pHC79 & RK2, Pseudomonas oriT
RA   Selvaraj G., Fong Y.C., Iyer V.N.;
RT   "A portable DNA sequence carrying the cohesive site (cos) of
RT   bacteriophage lambda and the mob (mobilization) region of the
RT   broad-host-range plasmid RK2: a module for the construction of
RT   new cosmids";
RL   Gene 32:235-241(1984).
XX
RN   [9]
RC   pADD948 from phage D3112
RA   Darzins A., Casadaban M.J.;
RT   "In vivo cloning of Pseudomonas aeruginosa genes with mini-D3112
RT   transposable bacteriophage";
RL   J. Bacteriol. 171:3917-3925(1989).
XX
RN   [10]
RC   Tn5-B12, Tn5-B13 from Tn5
RC   pJQ17 from pSUP5011 & Tn5-B12
RC   pJQ18 from pSUP5011 & Tn5-B13
RA   Quandt J.;
RT   ;
RL   Unpublished (1989).
XX
RN   [11]
RC   pRL6JI or pR1e3855a from Rhizobium
RC   pRL7JI from Rhizobium
RC   pRL8JI from Rhizobium
RC   pRL9JI from Rhizobium
RC   pRL10JI from Rhizobium
RC   pRL12JI from Rhizobium
RC   pRL13JI from Rhizobium
RA   Brewin N.J., Wood E.A., Johnston A.W., Dibb N.J., Hombrecher G.;
RT   "Recombinant nodulation plasmids in Rhizobium leguminosarum";
RL   J. Gen. Microbiol. 128:1817-1827(1982).
XX
RN   [12]
RC   pRme2011a, pRme2011b from Rhizobium
RA   Hynes M.F., Simon R., Muller P., Niehaus L., Labes M., Puhler A.;
RT   "The two megaplasmids of Rhizobium meliloti are involved in the
RT   effective nodulation of alfalfa";
RL   Mol. Gen. Genet. 202:356-362(1986).
XX
RN   [13]
RC   pR1eVF39a from Rhizobium
RA   Hynes M.F., Brucksch K., Priefer U.;
RT   "Melanin production encoded by a cryptic plasmid in a Rhizobium
RT   leguminosarum strain";
RL   Arch. Microbiol. 150:326-332(1988).
XX
RN   [14]
RC   pHSS8 from pHSS6 & M13
RA   Seifert H.S., So M., Heffron F.;
RT   "Shuttle mutagenesis: a method of introducing transposons into
RT   transformable organisms";
RL   Genet. Eng. 8:123-134(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   General purpose cloning vector containing a unique NotI site.
CC   Constructed for insertion of mobilization cassettes pMOB1-4
CC   (ATCC 77280-77283) to produce a vector useful for allele replacement
CC   in Pseudomonas aeruginosa.
CC   Also permits visual detection of recombinants by lacZalpha
CC   complementation.  The alpha peptide is 107 aa.
CC   The depositor requests that this plasmid be available only for
CC   non-commercial research purposes. (ATCC staff)
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--2.7; PvuI--1.75, 0.9; HindIII--2.7; NotI--2.7. (ATCC staff)
CC   Medium is 1315 1065 plus ampicillin (100 ug/ul).
CC   NM (pNOT19)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli DH5alphaF')(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 NdeI 2686bp 185..185, lacZalpha
FT                   blunt end:blunt end
FT                   2. NdeI-NotI linker 10bp gcggccgcta
FT                   -> pNOT19 2694bp"
FT   -               1..184
FT                   /note="pUC19 1..184 184bp
FT                   NdeI = CA^TATG
FT                   \         gcggccgcta"
FT   -               185..194
FT                   /note="gcggccgcta 10bp
FT                   \ gcggccgcta
FT                   NdeI = CA^TA TG"
FT   -               195..2694
FT                   /note="pUC19 187..2686 2500bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-XmaI-BamHI-SalI-
FT                   AccI-HincII-PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique NarI-NotI-PvuII-EcoRI-SacI-KpnI-
FT                   SmaI-XmaI-BamHI-SalI-AccI-HincII-PstI-SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
XX
SQ   Sequence 2694 BP; 666 A; 679 C; 690 G; 659 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accagcggcc gctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg agctcggtac
     ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc
     tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//