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ID   PNS1       preliminary; circular DNA; PRO; 3879 BP.
XX
AC   M16217;
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DT   02-APR-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus plasmid vector pNS1 - complete.
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KW   cloning vector.
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OS   Bacillus subtilis
OC   Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
OC   Micrococcaceae; Bacillaceae; Bacillus.
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RN   [1]
RP   1-3879
RC   pTP5 from Staphylococcus
RC   pNS1 from pTP5
RA   Noguchi N., Aoki T., Sasatsu M., Kono M., Shishido K., Ando T.;
RT   "Determination of the complete nucleotide sequence of pNS1, a
RT   staphylococcal tetracycline-resistance plasmid propagated in
RT   Bacillus subtilis";
RL   FEMS Microbiol. Lett. 37:283-288(1986).
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RN   [2]
RC   pNS1
RA   Kono M.;
RT   ;
RL   Submitted (23-JUN-1987) to GenBank on computer-readable media by:
RL   Kono M., .
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CC   NM (pNS1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (B.subtilis)(Staphylococcus)(Bacillus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pTP5)
CC   BR ()
CC   OF ()
CC   OR (XbaI site)
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Staphylococcus aureus
FT                   -> pTP5
FT                   1. pTP5 remove HindIII-HindIII 600bp, C fragment
FT                   -> pNS1 3879bp"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI"
FT   misc_binding    0..0
FT                   /note="SIT unique KpnI"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuI"
FT   CDS             305..1684
FT                   /note="ANT Staphylococcus tetracycline resistance gene
FT                   (tet); TTG start codon"
FT   rep_origin      0..0
FT                   /note="ORI Staphylococcus pTP5 (similar to pT181)"
XX
SQ   Sequence 3879 BP; 1391 A; 471 C; 688 G; 1329 T; 0 other;
     ctagaacaaa atataagaat ttgataaaag aaatttcgcc agtcgattta acggacttaa
     tgaaatcgac tttaaaagcg aacgaaaaac aattgcaaaa acaaatcgat ttttggcaac
     atgaatttaa attttggaaa tagtgtacat attaatatta ctgaacaaaa atgatatatt
     taaactattc taatttagga ggattttttt atgaagtgtc tatttaaaaa tttggggaat
     ttatatgagg tgaaagaata atttacccct ataaacttta gtcacctcaa gtaaagaggt
     aaaattgttt agtttatata aaaaatttaa aggtttgttt tatagcgttt tattttggct
     ttgtattctt tcatttttta gtgtattaaa tgaaatggtt ttaaatgttt ctttacctga
     tattgcaaat cattttaata ctactcctgg aattacaaac tgggtaaaca ctgcatatat
     gttaactttt tcgataggaa cagcagtata tggaaaatta tctgattata taaatataaa
     aaaattgtta attattggta ttagtttgag ctgtcttggt tcattgattg cttttattgg
     tcacaatcac ttttttattt tgatttttgg taggttagta caaggagtag gatctgctgc
     attcccttca ctgattatgg tggttgtagc tagaaatatt acaagaaaaa aacaaggcaa
     agcctttggt tttataggat caattgtagc tttaggtgaa gggttaggtc cttcaatagg
     gggaataata gcacattata ttcattggtc ttacctactt atacttccta tgattacaat
     agtaactata ccttttctta ttaaagtaat ggtacctggt aaatcaacaa aaaatacatt
     agatatcgta ggtattgttt taatgtctat aagtattata tgttttatgt tatttacgac
     aaattataat tggacttttt taatactctt cacaatcttt tttgtgattt ttattaaaca
     tatttcaaga gtttctaacc cttttattaa tcctaaacta gggaaaaaca ttccgtttat
     gcttggtttg ttttctggtg ggctaatatt ttctatagta gctggtttta tatcaatggt
     gccttatatg atgaaaacta tttatcatgt aaatgtagcg acaataggta atagtgttat
     ttttcctgga accatgagtg ttattgtttt tggttatttt ggtggttttt tagtggatag
     aaaaggatca ttatttgttt ttattttagg atcattgtct atctctataa gttttttaac
     tattgcattt tttgttgagt ttagtatgtg gttgactact tttatgttta tatttgttat
     gggcggatta tcttttacta aaacagttat atcaaaaata gtatcaagta gtctttctga
     agaagaagtt gcttctggaa tgagtttgct aaatttcaca agttttttat cagagggaac
     aggtatagca attgtaggag gtttattgtc actacaattg attaatcgta aactagttct
     ggaatttata aattattctt ctggagtgta tagtaatatt cttgtagcca tggctatcct
     tattatttta tgttgtcttt tgacgattat tgtatttaaa cgttctgaaa agcagtttga
     atagttatat tatattttgg tttagaacta tgagtggcta gcattttgcc actcattttt
     tgcgttagca aaaacaggtt taagcctcgc agagcacacg tattaacgac ttattaaaaa
     taagtctagt gtgttagact taaactatta aatacacatg aaacctttgt gcttaggagt
     gatttttata tgtcttattc cattgttaga gtttcaaaag ttaaatctgg aacaaataca
     acgggcatac aaaaacatgt tcaaagagaa aataataatt atgaaaatga agatatagac
     catagtaaaa cttacttaaa ttatgatttg gtaaatgcta ataaacagaa ttttaataac
     ttgattgatg aaaaaatcga acagaattat acaggcaaaa gaaaaattag aacagacgcg
     attaaacaca ttgatggttt aattacatca gacaatgatt tctttgataa tcaaacgcca
     gaagatacaa agcagttttt tgaatatgct aaagagtttt tagaacaaga atacggtaaa
     gataatttat tatatgcaac agttcacatg gacgaaaaaa caccacatat gcattatggc
     gttgttccaa taactgatga tggtcgttta agtgctaaag aagttgtagg taataaaaaa
     gcttttaaaa atcttactaa aagagttaga acgagtttta ggaagaaata cctttgcgga
     aagagttaat aagttaacag aagatgaacc aaaactaaat ggtttagcag gaaacttaga
     taaaaaaatg aatccagaat tatattcaga acaggaacag caacaagaac aacaaaagaa
     tcaaaaacga gatagaggta tgcacttata gaacatgcat ttatgccgag aaaacttatt
     ggttggaatg ggctatgtgt tagctaactt gttagcgagt tggttggact tgaattggga
     ttaatcccaa gaaagtacca acccaacaac acataaagcc ctgtaggttc cgaccaataa
     ggaaattgga ataaagcaat aaaaggagtt gaagaaatga aattcagaga agcctttgag
     aattttataa caagtaagta tgtacttggt gttttagtag ttttaactgt ttaccagata
     atacaaatgc ttaaataaaa aaagacttga tctgattaga ccaagtcttt tgatagtgtt
     atattaataa caaaataaaa aggagtcgct cacgccttga ccaaagtttg tgaacgacat
     cattcaaaga aaaaaacact gagttgtttt tataatcttg tatatttaga tattaaacga
     tatttaaata tacatcaaga tatatatttg ggtgagcgat tccttaaacg aaattgagat
     taaggagtcg attttttatg tataaaaaca atcatgcaaa tcattcaaat catttggaaa
     atcacgattt agacaatttt tctaaaaccg gctactctaa tagccggttg gacgcacata
     ctgtgtgcat atctgatcca aaattaagtt ttgatgcaat gacgatcgtt ggaaatctca
     accgagacaa cgctcaagcc ctttctaaat ttatgagtgt agagccccaa ataagacttt
     gggatattct tcaaacaaag tttaaagcta aagcacttca agaaaaagtt tatattgaat
     atgacaaagt gaaagcagat agttgggata gacgtaatat gcgtattgaa tttaatccaa
     acaaacttac acgagatgaa atgatttggt taaaacaaaa tataataagc tacatggaag
     atgacggttt tacaagatta gatttagcct ttgattttga agatgatttg agtgactact
     atgcaatgtc tgataaagca gttaagaaaa ctatttttta tggtcgtaat ggtaagccag
     aaacaaaata ttttggcgtg agagatagta atagatttat tagaatttat aataaaaagc
     aagaacgtaa agataatgca gatgctgaag ttatgtctga acatttatgg cgtgtagaaa
     tcgaacttaa aagagatatg gtggattact ggaatgattg ctttagtgat ttacatatct
     tgcaaccaga ttggaaaact atccaacgca ctgcggatag agcaatagtt tttatgttat
     tgagtgatga agaagaatgg ggaaagcttc acagaaatt
//