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ID   POM2       preliminary; circular DNA; SYN; 2716 BP.
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AC   L08938; VB0059; ATCC77221;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pOM2 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pUC830 from pUC8 & M13tg131
RC   pUC931 from pUC9 & M13tg131
RC   pOM1 from pUC8 & M13tg131
RC   pOM2 from pUC9 & M13tg131
RC   pOM3 from pUC8 & M13tg131
RC   pOM4 from pUC9 & M13tg131
RC   pOM8 from pUC8 & M13tg131
RC   pOM9 from pUC9 & M13tg131
RA   Oberbaumer I.;
RT   "New pUC-derived expression vectors for rapid construction of cDNA
RT   libraries";
RL   Gene 49:81-91(1986).
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RN   [2]
RC   from pOM series
RA   ;
RT   ;
RL   Focus 14:72-72(1992).
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RN   [3]
RP   1-2716
RC   pOM2
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled by F. Pfeiffer, MPI Biochemie, Martinsried
CC   Expression vector useful for construction of cDNA libraries and
CC   containing priming sites useful for sequencing.  Permits visual
CC   detection of recombinants by beta-galactosidase alpha peptide
CC   complementation. [1]
CC   pOM1 (ATCC 77220) and pOM2 (ATCC 77221) differ in the orientation of
CC   the polylinker. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   PstI--2.7; HindIII--2.7; EcoRI--2.7; XhoI--2.7. (ATCC staff)
CC   E.coli transformed with this plasmid produce larger colonies
CC   when an insert is present in the polylinker than when there is no
CC   insert. (personal communication)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pOM2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (M13tg130)(pUC8)(pUC830)(bGal130)
CC   BR (pOM1)(pOM3)(pOM4)(pOM5)(pOM6)(pOM7)(pOM8)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 HindIII 2665bp 261..261
FT                   Klenow
FT                   XhoI linker 6bp ctcgag
FT                   remove EcoRI-PstI 26bp 231..257, MCS/2650bp
FT                   2. M13tg131 PstI-EcoRI 59bp 6242..6301, MCS
FT                   -> pOM2 2716bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             984..1772
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    1..240
FT                   /note="from pUC8"
FT   misc_feature    230..298
FT                   /note="MCS EcoRI-SmaI-SacI-EcoRV-SphI-KpnI-XbaI-
FT                   HindIII-BamHI-SalI-PstI-XhoI; M13tg130-Polylinker"
FT   misc_feature    306..311
FT                   /note="SIT XhoI linker"
FT   misc_feature    312..2716
FT                   /note="from pUC8"
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SQ   Sequence 2716 BP; 667 A; 692 C; 685 G; 672 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcccggg
     agagctcgat atcgcatgcg gtacctctag aagaagcttg ggatccgtcg acctgcagcc
     aagctctcga gagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     gcgaggaagc ggaaga
//