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ID   POM3       preliminary; circular DNA; SYN; 2713 BP.
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AC   L08939; VB0060; ATCC77222;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pOM3 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pUC830 from pUC8 & M13tg131
RC   pUC931 from pUC9 & M13tg131
RC   pOM1 from pUC8 & M13tg131
RC   pOM2 from pUC9 & M13tg131
RC   pOM3 from pUC8 & M13tg131
RC   pOM4 from pUC9 & M13tg131
RC   pOM8 from pUC8 & M13tg131
RC   pOM9 from pUC9 & M13tg131
RA   Oberbaumer I.;
RT   "New pUC-derived expression vectors for rapid construction of cDNA
RT   libraries";
RL   Gene 49:81-91(1986).
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RN   [2]
RC   from pOM series
RA   ;
RT   ;
RL   Focus 14:72-72(1992).
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RN   [3]
RP   1-2713
RC   pOM3
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled by F. Pfeiffer, MPI Biochemie, Martinsried
CC   Expression vector useful for construction of cDNA libraries and
CC   containing priming sites useful for sequencing.  Permits visual
CC   detection of recombinants by beta-galactosidase alpha peptide
CC   complementation. [1]
CC   pOM3 (ATCC 77222) and pOM4 (ATCC 77223) differ in the orientation of
CC   the polylinker. [1]
CC   Contains an additional ATG in the inserted ClaI linker in a different
CC   reading frame which might also function as start codon for expression.
CC   [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   PstI--2.7; HindIII--2.7; EcoRI--2.7. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pOM3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (M13tg131)(pUC9)(pUC931)(bGal131)
CC   BR (pOM1)(pOM2)(pOM4)(pOM5)(pOM6)(pOM7)(pOM8)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC9 HindIII 2665bp 234..234
FT                   mung bean nuclease
FT                   ClaI linker 8bp natcgatn
FT                   remove PstI-EcoRI 18bp 246..264, MCS/2650bp
FT                   2. M13tg131 PstI-EcoRI 59bp 6242..6301, MCS
FT                   -> pOM3 2713bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             981..1769
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    1..233
FT                   /note="from pUC9"
FT   misc_feature    235..240
FT                   /note="SIT ClaI linker"
FT   misc_feature    248..316
FT                   /note="MCS ClaI-PstI-SalI-BamHI-HindIII-XbaI-KpnI-
FT                   SphI-EcoRV-SstI-SmaI-EcoRI; M13tg131-Polylinker"
FT   misc_feature    306..2713
FT                   /note="from pUC9"
XX
SQ   Sequence 2713 BP; 664 A; 693 C; 682 G; 674 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccacatcgat
     ggcttggctg caggtcgacg gatcccaagc ttcttctaga ggtaccgcat gcgatatcga
     gctctcccgg gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     aggaagcgga aga
//