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ID   POM8       preliminary; circular DNA; SYN; 2704 BP.
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AC   L08941; VB0062; ATCC77224;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pOM8 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pUC830 from pUC8 & M13tg131
RC   pUC931 from pUC9 & M13tg131
RC   pOM1 from pUC8 & M13tg131
RC   pOM2 from pUC9 & M13tg131
RC   pOM3 from pUC8 & M13tg131
RC   pOM4 from pUC9 & M13tg131
RC   pOM8 from pUC8 & M13tg131
RC   pOM9 from pUC9 & M13tg131
RA   Oberbaumer I.;
RT   "New pUC-derived expression vectors for rapid construction of cDNA
RT   libraries";
RL   Gene 49:81-91(1986).
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RN   [2]
RC   from pOM series
RA   ;
RT   ;
RL   Focus 14:72-72(1992).
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RN   [3]
RP   1-2704
RC   pOM8
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled by F. Pfeiffer, MPI Biochemie, Martinsried
CC   Expression vector useful for construction of cDNA libraries and
CC   containing priming sites useful for sequencing.  Permits visual
CC   detection of recombinants by beta-galactosidase alpha peptide
CC   complementation. [1]
CC   pOM8 (ATCC 77224) and pOM9 (ATCC 77225) differ in the orientation of
CC   the polylinker. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   PstI--2.7; HindIII--2.7; EcoRI--2.7; MunI--2.7. (ATCC staff)
CC   E.coli transformed with this plasmid produce larger colonies
CC   when an insert is present in the polylinker than when there is no
CC   insert. (personal communication)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pOM8)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (M13tg130)(pUC8)(pUC830)(bGal130)
CC   BR (pOM1)(pOM2)(pOM4)(pOM5)(pOM6)(pOM7)(pOM3)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 HindIII 2665bp 261..261
FT                   Klenow, [1 bp]
FT                   mung bean nuclease [3bp]
FT                   remove EcoRI-PstI 26bp 231..257, MCS/2650bp
FT                   2. M13tg131 PstI-EcoRI 59bp 6242..6301, MCS
FT                   -> pOM8 2704bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             972..1760
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    1..240
FT                   /note="from pUC8"
FT   misc_feature    230..298
FT                   /note="MCS EcoRI-SmaI-SacI-EcoRV-SphI-KpnI-XbaI-
FT                   HindIII-BamHI-SalI-PstI; M13tg130-Polylinker"
FT   misc_feature    303..2704
FT                   /note="from pUC8"
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SQ   Sequence 2704 BP; 665 A; 688 C; 681 G; 670 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcccggg
     agagctcgat atcgcatgcg gtacctctag aagaagcttg ggatccgtcg acctgcagcc
     aattggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     aaga
//