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ID   PON1       preliminary; circular DNA; SYN; 6708 BP.
XX
AC   IG9942;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pON1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pON1 from pBR322 & Tn5 neo gene
RA   Nielsen O.;
RT   ;
RL   Unpublished (1991).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pON1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(Tn5)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 SalI 4361bp 652..652
FT                   2. Tn5 XhoI-XhoI 2347bp 486..2833, neo gene
FT                   -> pON1 6708bp [6400bp]"
FT   -               1..651
FT                   /note="pBR322 1..651 651bp
FT                   SalI = G^TCGAC
FT                   XhoI = C^TCGAG"
FT   -               652..2998
FT                   /note="Tn5 486..2832 2347bp
FT                   XhoI = C^TCGAG
FT                   SalI = G^TCGAC"
FT   -               2999..6708
FT                   /note="pBR322 652..4361 3710bp"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 6708 BP; 1478 A; 1856 C; 1874 G; 1500 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaggcca
     ccacattccg caccgtagga ttactgcatc aggagtggtg gatgcgcccg gatgaccctg
     ccgatgcgga tgaaaaggag agtggcaaat ggctggcagc ggccgcaact agccggttac
     gcatgggcag catgatgagc aacgtgattg cggtctgtga ccgcgaagcc gatattcatg
     cttatctgca ggacaggctg gcgcataacg agcgcttcgt ggtgcgctcc aagcacccac
     gcaaggacgt agagtctggg ttgtatctga tcgaccatct gaagaaccaa ccggagttgg
     gtggctatca gatcagcatt ccgcaaaagg gcgtggtgga taaacgcggt aaacgtaaaa
     atcgaccagc ccgcaaggcg agcttgagcc tgcgcagtgg gcgcatcacg ctaaaacagg
     ggaatatcac gctcaacgcg gtgctggccg aggagattaa cccgcccaag ggtgagaccc
     cgttgaaatg gttgttgctg accggcgaac cggtcgagtc gctagcccaa gccttgcgcg
     tcatcgacat ttatacccat cgctggcgga tcgaggagtt ccataaggca tggaaaaccg
     gagcaggagc cgagaggcaa cgcatggagg agccggataa tctggagcgg atggtctcga
     tcctctcgtt tgttgcggtc aggctgttac agctcagaga aagcttcacg ctgccgcaag
     cactcagggc gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa
     cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc
     gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
     ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
     ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
     agatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     tcctccagcg cggggatctc atgctggagt tcttcgccca ccccgggctc gatcccctcg
     cgagttggtt cagctgctgc ctgaggctgg acgacctcgc ggagttctac cggcagtgca
     aatccgtcgg catccaggaa accagcagcg gctatccgcg catccatgcc cccgaactgc
     aggagtgggg aggcacgatg gccgctttgg tcgacccgga cgggacgctc ctgcgcctga
     tacagaacga attgcttgca ggcatctcat gagtgtgtct tcccgttttc cgcctgaggt
     cactgcgtgg atggagcgct ggcgcctgct gcgcgacggc gagctgctca ccacccactc
     gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
     ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
     catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac
     tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg
     gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag
     cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg
     ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag
     cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg
     tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg
     catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg
     gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc
     ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct
     ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc
     agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt
     aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc
     gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg
     tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaa
//