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ID   PON163     preliminary; circular DNA; SYN; 7420 BP.
XX
AC   IG1097;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pON163 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pON3 from pBR322 & pON1
RC   pON5 from pON3 & lambda
RC   pON163 from pON5 & ARS1
RC   pDW222, pDW225 from HIS2 gene & URA gene
RC   pDW227 from pGEM-3 & pON163
RC   pDW229 from pGEM-3 & pON163
RC   pDW232 from pGEM-3 & pDW227 & pDW229
RA   Weilguny D., Praetorius M., Carr A.M., Egel R., Nielsen O.;
RT   "New vectors in fission yeast: application for cloning the his2
RT   gene";
RL   Gene 99:47-54(1991).
XX
RN   [2]
RC   pON1 from pBR322 & Tn5 neo gene
RA   Nielsen O.;
RT   ;
RL   Unpublished (1991).
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CC   NM (pON163)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli)(S.cerevisiae)(S.pombe)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 SalI 4361bp 652..652
FT                   2. Tn5 XhoI-XhoI 2347bp 486..2833, neo gene
FT                   -> pON1 6400bp
FT                   1. pBR322 remove HindIII-PvuII 2037bp 30..2067,
FT                   \ tet gene/2324bp
FT                   2. pON1 HindIII-SmaI 1323bp 1196..2519, Tn5 neo gene
FT                   -> pON3 3647bp
FT                   1. pON3 remove small ClaI-BclI 1100bp,
FT                   \ 25..30/1774..2768
FT                   \ pBR322 ClaI 25/pSV2neo BclI 2768/2200bp
FT                   2. lambda BglII-BglII 2393bp, rexAB/cI gene/pR prom
FT                   ClaI-BglII, cI gene/pR promoter
FT                   -> pON5 4600bp
FT                   1. pON5 EcoRI 4600bp
FT                   2. yeast EcoRI-EcoRI 1100bp, ARS1
FT                   -> plasmid 5700bp
FT                   1. plasmid ClaI 5700bp
FT                   2. S. pombe HindIII-HindIII 1700bp, URA4 gene
FT                   Klenow:Klenow
FT                   ClaI linker 6bp atcgat:ClaI linker 6bp atcgat
FT                   ClaI-ClaI 1700bp, URA4 gene
FT                   -> pON163 7420bp"
FT   CDS             complement(0..0)
FT                   /note="REP bacteriophage lambda cI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT BclI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PR; rightward"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   CDS             complement(0..0)
FT                   /note="ANT yeast URA4 gene"
XX
SQ   Sequence 7420 BP; 2122 A; 1657 C; 1562 G; 2079 T; 0 other;
     attttatgaa tatacaaata attggagcca acctgcaggt gatgattatc agccagcaga
     gaattaagga aaacagacag gtttattgag cgcttatctt tccctttatt tttgctgcgg
     taagtcgcat aaaaaccatt cttcataatt caatccattt actatgttat gttctgaggg
     gagtgaaaat tcccctaatt cgatgaagat tcttgctcaa ttgttatcag ctatgcgccg
     accagaacac cttgccgatc agccaaacgt ctcttcaggc cactgactag cgataacttt
     ccccacaacg gaacaactct cattgcatgg gatcattggg tactgtgggt ttagtggttg
     taaaaacacc tgaccgctat ccctgatcag tttcttgaag gtaaactcat cacccccaag
     tctggctatg cagaaatcac ctggctcaac agcctgctca gggtcaacga gaattaacat
     tccgtcagga aagcttggct tggagcctgt tggtgcggtc atggaattac cttcaacctc
     aagccagaat gcagaatcac tggctttttt ggttgtgctt acccatctct ccgcatcacc
     tttggtaaag gttctaagct caggtgagaa catccctgcc tgaacatgag aaaaaacagg
     gtactcatac tcacttctaa gtgacggctg catactaacc gcttcataca tctcgtagat
     ttctctggcg attgaagggc taaattcttc aacgctaact ttgagaattt ttgcaagcaa
     tgcggcgtta taagcattta atgcattgat gccattaaat aaagcaccaa cgcctgactg
     ccccatcccc atcttgtctg cgacagattc ctgggataag ccaagttcat ttttcttttt
     ttcataaatt gctttaaggc gacgtgcgtc ctcaagctgc tcttgtgtta atggtttctt
     ttttgtgctc atacgttaaa tctatcaccg caagggataa atatctaaca ccgtgcgtgt
     tgactatttt acctctggcg gtgataatgg ttgcatgtac taaggaggtt gtatggaaca
     acgcataacc ctgaaagatt atgcaatgcg ctttgggcaa accaagacag ctaaagatca
     agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     ggggatctca tgctggagtt cttcgcccac cccctgcctc gcgcgtttcg gtgatgacgg
     tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt cgagtctaac
     tccttaacca ctcggacata gtgacttatc tgacactcat tgtaaattaa tatatatata
     ggcattttgt ttagttaaag gtacttaagt aattagtata aacgaaccaa ttttataatc
     aggaagttaa gtgaatggta gcacatgtcg taaaaattgt gaatttttat tgaataatat
     tttaaataca agcctttcta gactaggtat actcataaac atatgagcaa aaggatagag
     gagattacat tgcatcttct acaaatttat ttattgccct ttactgaaaa attaaataat
     gagtactaaa tgataaaaag cgctcagtac aagaaagatt gcaaaaatat tgcattcttc
     atgaattaaa gttgcatata aggcatattg aaagtaatag tactaaaaca gcagttagcg
     aaaattaata gaattatatt cgcaagacaa ttgtgacatt aaattaaaaa attgtagaat
     ttttactatc ctctttaacg ccatgagcct ttataaaaag gttaaattag ttttaacatt
     ctttttttga gtaagagttt acaatttatc aaaacctgtg ttattatatt cattagtttc
     aatttattag catctagaga aaaatcaatt ggcagttaca ttgttggaat ttatgaagaa
     aaagaatcta caacggagaa taagttgctg atcgctttcc ctaaaattgt atattttgct
     gagcttattt tgacatttcg ttgaagtttt ctctaattcg cattcatttt aagtaaaaca
     atgagaaata aaattacaaa aaatacaaat taaaatacaa tttttagcta taattataga
     cgatgccctt gtatcccatt ctgtctcgct tgcccctact ttttatcttt tatataccat
     aatgaacgct gccgctacta accatacccc gattttacat ttcggactcc caaggacgta
     caaaatagaa aactatagaa aaaaataatc agaaaatagc atgtcatctc tttgtaaaac
     gcgtttgcaa gaagaaagga aacaatggag aagagatcat ccatttgtat gtaaatttta
     gtaaacttga agaaatcact aacaacttct cttacttagg gattctatgc aaaaccttgt
     aaatcatctg atggaggact cgatttaatg aattggaagg ttggaattct catgtttgac
     agcttatcat cgatagcttg tgatattgac gaaacttttt gacatctaat ttattctgtt
     ccaacaccaa tgtttataac caagttttat cttgtttgtc tacatggtat tttacattca
     tctacataca tctttcattg gctttgtaca tagttatcat tacaagtcta aaaaaattca
     ctcttttctt attcaatgtc aatccaagag aaaagattgt ggtaatgttg taggagcatg
     tttaataaat tactatagca aattactttt tattcccaag gtgtttatct ataatagtta
     atattttagt cgctacataa aattttacca aagagtactt gtatactaat tctaaatgcc
     ttctgacata aaacgcctag gaaaacaaac gcaaacaagg catcgacttt ttcaataacc
     aaccaaaaaa attttacatt agtctttttt taatgctgag aaagtctttg ctgatatgcc
     ttccaaccag cttctctata tctcttggct tcgacaacag gattacgacc agctccatag
     actccacgac caacaatgat gatatcgcta ccgcagttta caatcacttc ttcaggagta
     cgatattgct gtcccagccc gtctccttta acatccaagc cgataccagg ggacatagtt
     atgtagtcgc tttgaaggtt aggaaatcga cgaccagcta taaagccaaa gcaaaaatcg
     gtatgcttct caaaccattc taaggttttc tctgtgtagg aaccagtagc caaagagcct
     ttggaagaca tttcagccaa aagcaagaga ccacgtccca aaggtaaacc aacttctttg
     aggccttgta taataccctc gcctggcact gtatggcaat ttgtgatatg agcccaagaa
     gcaattttgt acacaccaga tgcatattgt agcttgacgg tatttccaat gtctgcgaat
     ttgcgatcct caaagataag aaaacgatgc tttttaccta aggccaccag tttttctacc
     atatcctggt cgaaatcctc gacaacgtca atatgtgtct tgataacaca gacatagggt
     ccaattttat ctaccaattc taagatttcg gatttcttcg tcaaatcgac cgcgactgac
     aagttgcttt gcttttcttc catcaaagcc aacaattcct tggcaatggg atttttcatc
     ccctcagctc tagctgaata gctttgaaat actctagcat ccataacttt gcttttaaac
     ctttaatttc gatccaagca aaaaagaggt tcttggtagg acaatacggt aagaaaacac
     gacatgtgca gagatgccga cgaagcatag ttaaactggg atggtaaaat caattaagaa
     tttataaaga caaaattgta taagtctcta aaacatctta attatacctc acagaactat
     ctaaaatata ttcacaaagt gcaaacatta tcatgaaaaa gaaccatttt aatttaaagc
     aagggcatta aggcttattt acagaatttc ttacttttgt aaagattata aggctgatta
     tctttttcac catgccaaaa attacacaag atagaatgga tgtttgaaat taaacgtgag
     tatacaaaca aatacactag gtaaatcgaa acattttttt ctccattaag taacaaattc
     ctatttagag aaagaatgct gagtagatta aataatctat acaaactttt ttaacacaaa
     tgcatacata tagccagtgg gatttgtagc taagctatcg
//