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ID   PON3       preliminary; circular DNA; SYN; 3647 BP.
XX
AC   IG9943;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pON3 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pON3 from pBR322 & pON1
RC   pON5 from pON3 & lambda
RC   pON163 from pON5 & ARS1
RC   pDW222, pDW225 from HIS2 gene & URA gene
RC   pDW227 from pGEM-3 & pON163
RC   pDW229 from pGEM-3 & pON163
RC   pDW232 from pGEM-3 & pDW227 & pDW229
RA   Weilguny D., Praetorius M., Carr A.M., Egel R., Nielsen O.;
RT   "New vectors in fission yeast: application for cloning the his2
RT   gene";
RL   Gene 99:47-54(1991).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pON3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(Tn5)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 SalI 4361bp 652..652
FT                   2. Tn5 XhoI-XhoI 2347bp 486..2833, neo gene
FT                   -> pON1 6708bp [6400bp]
FT                   1. pBR322 remove HindIII-PvuII 2037bp 30..2067,
FT                   \ tet gene/2324bp
FT                   2. pON1 HindIII-SmaI 1323bp 1196..2519, Tn5 neo gene
FT                   -> pON3 3647bp"
FT   -               1..29
FT                   /note="pBR322 1..29 29bp
FT                   HindIII = A^AGCTT"
FT   -               30..1352
FT                   /note="Tn5 1196..2518 1323bp
FT                   SmaI = XmaI = CCC^GGG
FT                   PvuII =       CAG^CTG"
FT   -               1353..3647
FT                   /note="pBR322 2067..4361 2295bp"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3647 BP; 860 A; 948 C; 986 G; 853 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gcttcacgct gccgcaagca ctcagggcgc
     aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc
     cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
     caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
     agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
     aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa
     gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     gggatctcat gctggagttc ttcgcccacc ccctgcctcg cgcgtttcgg tgatgacggt
     gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaa
//