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ID   PPB1       preliminary; circular DNA; SYN; 2666 BP.
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AC   IG5182; ATCC77473;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pPB1 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pPB1 from pKK233-2 & pUC19
RC   pPB14 from pDT14 & pPB1
RC   pAXB from pKK233-2 & pEE605 & pHL413
RC   pXPB from pAXB & pPB1
RA   Beernink P.T., Tolan D.R.;
RT   "Construction of a high-copy ATG vector for expression in
RT   Escherichia coli";
RL   Protein Expr. Purif. 3:332-336(1992).
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RN   [2]
RC   pDT14 from trc promoter & aldolase A gene
RA   Morris A., Tolan D.;
RT   "Site-directed mutagenesis identifies aspartate 33 as a previously
RT   unidentified critical residue in the catalytic mechanism of
RT   rabbit aldolase A";
RL   J. Biol. Chem. 268:1095-1100(1992).
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RN   [3]
RC   pEE313, pEE201, pEE605, pSE605 from human aldolase A gene
RC   pPE313, pEB313, pEB201, pBE313 from human aldolase A gene
RC   pXE313, pXX313, pSK313, pSS405, pKK607 from aldolase A gene
RC   p6-OPP403, p54PP403 from aldolase A gene
RA   Tolan D.R., Penhoet E.E.;
RT   "Characterization of the human aldolase B gene";
RL   Mol. Biol. Med. 3:245-264(1986).
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RN   [4]
RC   pHL413, pHL216, pHL201 from pBR322 & Charon 4A & aldolase A gene
RC   lambda HG313 from Charon 4A & human liver DNA aldolase B gene
RA   Rottman W.H., Tolan D.R., Penhoet E.E.;
RT   "Complete amino acid sequence for human aldolase B derived from
RT   cDNA and genomic clones";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:2738-2742(1984).
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RN   [5]
RC   pGJ9 from pACYC184 & pLC15-48, mtlA gene
RC   pGJ9-deltaSnaBI from pGJ9
RC   pPG1 from pCD7.5
RA   Grisafi P.L., Scholle A., Sugiyama J., Briggs C., Jacobson G.J.,
RA   Lengeler J.W.;
RT   "Deletion mutants of the Escherichia coli K-12 mannitol permease:
RT   dissection of transport-phosphorylation, phospho-exchange, and
RT   mannitol-binding activities";
RL   J. Bacteriol. 171:2719-2727(1989).
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RN   [6]
RC   pCD7.5 from mannitol operon
RA   Lee C.A., Saier M.H.;
RT   "Use of cloned mtl genes of Escherichia coli to induce mtl deletion
RT   mutations into the chromosome";
RL   J. Bacteriol. 153:685-692(1983).
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RN   [7]
RC   pLC15-48 from ColE1 & mtl genes
RA   Lee C.A., Jacobson G.R., Saier M.H.;
RT   "Plasmid-directed synthesis of enzymes required for D-mannitol
RT   transport and utilization in Escherichia coli";
RL   Proc. Natl. Acad. Sci. U.S.A. 78:7336-7340(1981).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   Expression vector. Permits high copy number and production of
CC   proteins initiated at the ATG of beta-galactosidase.
CC   The order of the major features in the plasmid is:  pMB1 ori -
CC   EcoRI - trc promoter - lacZ'/NcoI - PstI - HindIII - ampR. [1]
CC   Deposited by: D.R.Tolan
CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   NM (pPB1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA (pUC19)(pKK233-2)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKK233-2 ScaI-EcoRI 1113bp 3477..4590
FT                   :Klenow
FT                   2. pUC19 PvuII-ScaI 1549bp 631..2180
FT                   -> pPB1 2662bp"
FT   -               1..1117
FT                   /note="pKK233-2 3477..4593 1117bp
FT                   EcoRI = G^AATT C
FT                   PvuII =    CAG^CTG"
FT   -               1118..2666
FT                   /note="pUC19 631..2179 1549bp
FT                   ScaI = AGT^ACT"
FT   promoter        0..0
FT                   /note="PRO trc"
FT   terminator      0..0
FT                   /note="TER rrnB"
FT   misc_binding    0..0
FT                   /note="SIT NcoI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ORI E. coli amp gene"
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SQ   Sequence 2666 BP; 663 A; 687 C; 641 G; 675 T; 0 other;
     actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     gcggatacat atttgaatgt atttagaaaa ataaacaaaa agagtttgta gaaacgcaaa
     aaggccatcc gtcaggatgg ccttctgctt aatttgatgc ctggcagttt atggcgggcg
     tcctgcccgc caccctccgg gccgttgctt cgcaacgttc aaatccgctc ccggcggatt
     tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
     cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgca tggggagacc
     ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga
     ccaccgcgct actgccgcca ggcaaactgt tttatcagac cgcttctgcg ttctgattta
     atctgtatca ggctgaaaat cttctctcat ccgccaaaac agccaagctt ggctgcagcc
     atggtctgtt tcctgtgtga aattgttatc cgctcacaat tccacacatt atacgagccg
     gatgattaat tgtcaacagc tcatttcaga atatttgcca gaaccgttta tatgtcggcg
     caaaaaacat tatccagaac gggagtgcgc cttgagcgac acgaattatg cagtgattta
     cgacctgcac agccaatcca cagcttccga tggctgcctg acgccagaag cattggtgca
     ccgtgcagtc gatgataagc tgtcaaacat gagaattctg cattaatgaa tcggccaacg
     cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     aagatgcttt tctgtgactg gtgagt
//