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ID   PPD127     preliminary; circular DNA; SYN; 6476 BP.
XX
AC   M34296;
XX
DT   03-JUL-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pPD1.27 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6476
RC   pPD1.27 from pAST19b & pCH110 & oligo
RC   pPD8.02 from pPD1.27 & oligo
RC   pPD8.33 from pPD8.02 & C.elegans unc-54 gene
RC   pPD15.08 from pPD8.02
RC   pPD16.43 from pPD8.02 & pPD15.08
RC   pPD16.51 from pPD8.33 & pPD15.08
RC   pPD18.32 from pPD16.51 & oligo
RC   pPD21.28 from pPD18.32 & pPD16.43
RC   pPD22.11 from pPD21.28
RC   pPD22.04 from pPD21.28
RC   pPD16.01 from pPD16.43 & pPD18.32
RC   pPD25.47 from pBluescript KS+ & glp-1 gene
RC   pPD26.77 from pPD16.43 & pPD25.47
RC   pPD34.110 from pPD21.28 & oligo
RA   Fire A.Z., Harrison S., Dixon D.;
RT   "A modular set of lacZ fusion vectors for studying gene expression
RT   in Caenorhabditis elegans";
RL   Gene 93:189-198(1990).
XX
RN   [2]
RC   pPD1.27
RA   Fire A.Z.;
RT   ;
RL   Submitted (11-MAY-1990) to GenBank on computer-readable media by:
RL   Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL   115 West University Parkway, Baltimore MD 21210, USA.
XX
CC   NM (pPD1.27)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pBR322)
CC   BR (pPPD16.01)(pPPD16.43)(pPPD16.51)(pPPD18.32)(pPPD21.28)(pPPD22.04)
CC   BR (pPPD22.11)(pPPD26.14)(pPPD26.77)(pPPD34.110)(pPPD8.02)(pPPD8.33)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pAST19b remove NarI-SacI 70bp 237..407,
FT                   \ 3432bp
FT                   2. oligo SacI-KpnI
FT                   3. pCH110 remove KpnI-BamHI 3528bp 209..3737,
FT                   \ lacZ gene/3600bp
FT                   4. oligo BamHI-NarI
FT                   -> pPD1.27 6476bp"
FT   misc_recomb     57..58
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     102..103
FT                   /note="synthetic end/E. coli trpS start"
FT   misc_recomb     186..187
FT                   /note="E. coli trpS end/synthetic start"
FT   misc_recomb     190..191
FT                   /note="synthetic end/E. coli lacZ start"
FT   misc_recomb     3500..3501
FT                   /note="E. coli lacZ end/SV40 start"
FT   misc_recomb     3634..3635
FT                   /note="SV40 end/synthetic start"
FT   misc_recomb     3657..3658
FT                   /note="synthetic end/pUC19 start"
FT   misc_recomb     4076..4077
FT                   /note="pUC19 end/C. elegans sup-7 start"
FT   misc_recomb     4442..4443
FT                   /note="C. elegans sup-7 end/pUC19 start"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 6476 BP; 1560 A; 1646 C; 1676 G; 1594 T; 0 other;
     atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta
     ccgagctcag aaaaaatgac tgctccaaag aagaagcgta aggtaccggt gggtgaagac
     cagaaacagc acctcgaact gagccgcgat attgcccagc gtttcaacgc gctgtatggc
     gagatcgatc ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
     gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc
     gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc
     tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
     ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
     ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac
     agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc
     gcggtgatgg tgctgcgttg gagtgacggc agttatctgg aagatcagga tatgtggcgg
     atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat
     ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt
     cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa
     acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt
     tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa
     atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa
     gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg
     aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt
     caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt
     aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc
     tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat
     cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg
     cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac
     ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg
     cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg
     cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt
     tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac
     agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc
     ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg
     tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac
     ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag
     cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc
     cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca
     agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa
     ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac
     gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa
     aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa
     atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt
     ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc
     acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac
     gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag
     tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat
     caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg
     gcgattaccg ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg
     aactgccagc tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa
     aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac
     atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg
     aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa
     cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg
     aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg
     gcggaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa
     taataaccgg gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat tatgtactat
     ttaaaaaaca caaacttttg gatgttcggt ttattctttt tcttttactt ttttatcatg
     ggagcctact tcccgttttt cccgatttgg ctacatgaca tcaaccatat cagcaaaagt
     gatacgggta ttatttttgc cgctatttct ctgttctcgc tattattcca accgctgttt
     ggtctgcttt ctgacaaact cggaacttgt ttattgcagc ttataatggt tacaaataaa
     gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     tgtccaaact catcaatgta tcttatcatg tctggatcga caaagtcaaa gcggccgcct
     gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatacaa
     ttttcagaat acgttttttg tgggcttggg tatattgttt ttaatgttat acttgcagtc
     gtgaaatttg attttcaaat ttgtagaaaa atcaagaaaa taattgcaac attcgcttgt
     gtcaaaaacc aatttcaaca aattttcgtg tgagaaatac attaccagaa ggcatttttt
     cacacgatta gcattttgga ctactttatt aaatttttgc gtgtaatttt gaattaaatt
     gtattatatt actacttaaa aaacaaaaaa tttgaccact gagcggatcg aacgcccaac
     ctttcgatct agagtcgaac gcgctaccat tgcgccaagc agtcatgtta ttctctcttg
     tcattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagct
//