back Return to this vector's summary.
ID   PPD1651    preliminary; circular DNA; SYN; 7010 BP.
XX
AC   M34300;
XX
DT   03-JUL-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pPD16.51 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-7010
RC   pPD1.27 from pAST19b & pCH110 & oligo
RC   pPD8.02 from pPD1.27 & oligo
RC   pPD8.33 from pPD8.02 & C.elegans unc-54 gene
RC   pPD15.08 from pPD8.02
RC   pPD16.43 from pPD8.02 & pPD15.08
RC   pPD16.51 from pPD8.33 & pPD15.08
RC   pPD18.32 from pPD16.51 & oligo
RC   pPD21.28 from pPD18.32 & pPD16.43
RC   pPD22.11 from pPD21.28
RC   pPD22.04 from pPD21.28
RC   pPD16.01 from pPD16.43 & pPD18.32
RC   pPD25.47 from pBluescript KS+ & glp-1 gene
RC   pPD26.77 from pPD16.43 & pPD25.47
RC   pPD34.110 from pPD21.28 & oligo
RA   Fire A.Z., Harrison S., Dixon D.;
RT   "A modular set of lacZ fusion vectors for studying gene expression
RT   in Caenorhabditis elegans";
RL   Gene 93:189-198(1990).
XX
RN   [2]
RC   pPD16.51
RA   Fire A.Z.;
RT   ;
RL   Submitted (11-MAY-1990) to GenBank on computer-readable media by:
RL   Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL   115 West University Parkway, Baltimore MD 21210, USA.
XX
CC   NM (pPD16.51)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pBR322)
CC   BR (pPD16.01)(pPD16.43)(pPD1.27)(pPD18.32)(pPD21.28)(pPD22.04)
CC   BR (pPD22.11)(pPD26.14)(pPD26.77)(pPD34.110)(pPD8.02)(pPD8.33)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pPD8.02 remove ?RsaI-HincII 366bp, sup7 gene
FT                   \ 5730bp
FT                   \ HincII = 39 607 1231 3059
FT                   -> pPD15.08 5730bp
FT                   1. pPD8.33 remove SspI-SspI 3071bp 1411..4482,
FT                   \  sup7 gene/4305bp
FT                   2. pPD15.08 SspI-SspI 1411..1411,
FT                   \ 5' MCS/3'MCS/no sup7 gene
FT                   -> pPD16.51 7010bp"
FT   misc_recomb     57..58
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     102..103
FT                   /note="synthetic end/E. coli trpS start"
FT   misc_recomb     186..187
FT                   /note="E. coli trpS end/synthetic start"
FT   misc_recomb     190..191
FT                   /note="synthetic end/E. coli lacZ start"
FT   misc_recomb     3184..3185
FT                   /note="E. coli lacZ end/synthetic start"
FT   misc_recomb     3244..3245
FT                   /note="synthetic end/unknown DNA start"
FT   misc_recomb     4524..4525
FT                   /note="unknown DNA end/synthetic start"
FT   misc_recomb     4544..4545
FT                   /note="synthetic end/pUC19 start"
FT   misc_recomb     4793..4794
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     4804..4805
FT                   /note="synthetic end/pUC19 start"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 7010 BP; 1753 A; 1710 C; 1778 G; 1769 T; 0 other;
     atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta
     ccgagctcag aaaaaatgac tgctccaaag aagaagcgta aggtaccggt gggtgaagac
     cagaaacagc acctcgaact gagccgcgat attgcccagc gtttcaacgc gctgtatggc
     gagatcgatc ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
     gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc
     gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc
     tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
     ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
     ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac
     agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc
     gcggtgatgg tgctgcgttg gagtgacggc agttatctgg aagatcagga tatgtggcgg
     atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat
     ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt
     cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa
     acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt
     tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa
     atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa
     gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg
     aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt
     caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt
     aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc
     tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat
     cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg
     cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac
     ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg
     cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg
     cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt
     tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac
     agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc
     ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg
     tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac
     ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag
     cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc
     cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca
     agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa
     ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac
     gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa
     aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa
     atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt
     ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc
     acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac
     gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag
     tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat
     caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg
     gcgattaccg ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg
     aactgccagc tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa
     aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac
     atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg
     aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa
     cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg
     aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg
     gcggaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa
     taggggccgc tgtcatcaga tcgccatctc gcgcccgtgc ctctgacttc taagtccaat
     tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat
     tatcaaaaaa acttcttctt aatttctttg ttttttagct tcttttaagt cacctctaac
     aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg
     tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca
     cttcttatgt tttttttact tctgataaat tttttttgaa acatcataga aaaaaccgca
     cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc
     acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt
     tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg
     ggtttcccct attgtttgtc aagagtttcg aggacggcgt ttttcttgct aaaatcacaa
     gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
     aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
     tgacagaata cattcccaat ataccaaaca taactgttta aaattaaaca tttttctaaa
     ttttatatga tttcttttaa atttgcaaaa attacttaaa tttgaattcc cgcgcaaatg
     agtgacttca ttttctgcat tattgtgttt tccggctata ttaataggta tttgtttgtg
     tttttcttta ttttatgatt cgaactccaa tttgtaaatt ttcgaacata tttccctaaa
     gaaaaaatat gattaatctg gaaaaattgg aaaattattt ttcaaataaa aaacaaagaa
     aaaaatgaag aaaaacctat tagtttggcc ataaaacgca aaaatgtcga aaatgacgtc
     actcatctgc gcgggaaatc aagaataatt cggccttttt tatttttttg gaaaatcgta
     aaacatttag aaaaattttt taatagttat agtgggactg tattctgtca tttagggcaa
     aagccagaga cgctactcca ccgttaacat gaattatgaa tattattgcg acaagaccca
     aacattgata aaccgcaaat ctagcctact agtcggccgt acgggccctt tcgtctcgcg
     cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa
     gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg
     agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
//