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ID   PPD1832    preliminary; circular DNA; SYN; 7088 BP.
XX
AC   M34301;
XX
DT   03-JUL-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pPD18.32 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-7088
RC   pPD1.27 from pAST19b & pCH110 & oligo
RC   pPD8.02 from pPD1.27 & oligo
RC   pPD8.33 from pPD8.02 & C.elegans unc-54 gene
RC   pPD15.08 from pPD8.02
RC   pPD16.43 from pPD8.02 & pPD15.08
RC   pPD16.51 from pPD8.33 & pPD15.08
RC   pPD18.32 from pPD16.51 & oligo
RC   pPD21.28 from pPD18.32 & pPD16.43
RC   pPD22.11 from pPD21.28
RC   pPD22.04 from pPD21.28
RC   pPD16.01 from pPD16.43 & pPD18.32
RC   pPD25.47 from pBluescript KS+ & glp-1 gene
RC   pPD26.77 from pPD16.43 & pPD25.47
RC   pPD34.110 from pPD21.28 & oligo
RA   Fire A.Z., Harrison S., Dixon D.;
RT   "A modular set of lacZ fusion vectors for studying gene expression
RT   in Caenorhabditis elegans";
RL   Gene 93:189-198(1990).
XX
RN   [2]
RC   pPD18.32
RA   Fire A.Z.;
RT   ;
RL   Submitted (11-MAY-1990) to GenBank on computer-readable media by:
RL   Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL   115 West University Parkway, Baltimore MD 21210, USA.
XX
CC   NM (pPD18.32)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pBR322)
CC   BR (pPD16.01)(pPD16.43)(pPD16.51)(pPD1.27)(pPD21.28)(pPD22.04)
CC   BR (pPD22.11)(pPD26.14)(pPD26.77)(pPD34.110)(pPD8.02)(pPD8.33)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pPD16.51 SmaI 7010bp 56..56, MCS
FT                   2. oligo SmaI-SmaI 78bp
FT                   -> pPD18.32 7088bp"
FT   misc_recomb     57..58
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     180..181
FT                   /note="synthetic end/E. coli trpS start"
FT   misc_recomb     264..265
FT                   /note="E. coli trpS end/synthetic start"
FT   misc_recomb     268..269
FT                   /note="synthetic end/E. coli lacZ start"
FT   misc_recomb     3262..3263
FT                   /note="E. coli lacZ end/synthetic start"
FT   misc_recomb     3322..3323
FT                   /note="synthetic end/unknown DNA start"
FT   misc_recomb     4602..4603
FT                   /note="unknown DNA end/synthetic start"
FT   misc_recomb     4622..4623
FT                   /note="synthetic end/pUC19 start"
FT   misc_recomb     4871..4872
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     4882..4883
FT                   /note="synthetic end/pUC19 start"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 7088 BP; 1780 A; 1724 C; 1797 G; 1787 T; 0 other;
     atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggat
     tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
     gaggaccctt ggagggtacc gagctcagaa aaaatgactg ctccaaagaa gaagcgtaag
     gtaccggtgg gtgaagacca gaaacagcac ctcgaactga gccgcgatat tgcccagcgt
     ttcaacgcgc tgtatggcga gatcgatccc gtcgttttac aacgtcgtga ctgggaaaac
     cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt
     cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc
     atctacacca acgtaaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat
     ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag
     acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg
     gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc
     gccggagaaa accgcctcgc ggtgatggtg ctgcgttgga gtgacggcag ttatctggaa
     gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg
     actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct
     gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt
     tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt
     atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg
     aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc
     gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa
     aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag
     catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg
     atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg
     tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac
     ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa
     cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg
     gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc
     gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt
     atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg
     tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac
     gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag
     tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat
     gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat
     cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg
     gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg
     accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg
     gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag
     ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta
     cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcctggcag
     cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca cgccatcccg
     catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg ttggcaattt
     aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg
     ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg
     acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc
     gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct gattacgacc
     gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt
     gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga tacaccgcat
     ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt aaactggctc
     ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg
     gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc
     tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac
     atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg
     gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc
     tggagcccgt cagtatcggc ggaattccaa ctgagcgccg gtcgctacca ttaccaactt
     gtctggtgtc aaaaataata ggggccgctg tcatcagatc gccatctcgc gcccgtgcct
     ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc
     accccctatt tttgttatta tcaaaaaaac ttcttcttaa tttctttgtt ttttagcttc
     ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata
     aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta
     cacaatgttc tgtgtacact tcttatgttt tttttacttc tgataaattt tttttgaaac
     atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg
     caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa
     aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa attaattttc
     ctgcttttgc tttttggggg tttcccctat tgtttgtcaa gagtttcgag gacggcgttt
     ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg
     ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta
     tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata actgtttaaa
     attaaacatt tttctaaatt ttatatgatt tcttttaaat ttgcaaaaat tacttaaatt
     tgaattcccg cgcaaatgag tgacttcatt ttctgcatta ttgtgttttc cggctatatt
     aataggtatt tgtttgtgtt tttctttatt ttatgattcg aactccaatt tgtaaatttt
     cgaacatatt tccctaaaga aaaaatatga ttaatctgga aaaattggaa aattattttt
     caaataaaaa acaaagaaaa aaatgaagaa aaacctatta gtttggccat aaaacgcaaa
     aatgtcgaaa atgacgtcac tcatctgcgc gggaaatcaa gaataattcg gcctttttta
     tttttttgga aaatcgtaaa acatttagaa aaatttttta atagttatag tgggactgta
     ttctgtcatt tagggcaaaa gccagagacg ctactccacc gttaacatga attatgaata
     ttattgcgac aagacccaaa cattgataaa ccgcaaatct agcctactag tcggccgtac
     gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     cgcatcaggc ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     aaacagct
//