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ID   PPD2128    preliminary; circular DNA; SYN; 6563 BP.
XX
AC   M34302;
XX
DT   03-JUL-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pPD21.28 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6563
RC   pPD1.27 from pAST19b & pCH110 & oligo
RC   pPD8.02 from pPD1.27 & oligo
RC   pPD8.33 from pPD8.02 & C.elegans unc-54 gene
RC   pPD15.08 from pPD8.02
RC   pPD16.43 from pPD8.02 & pPD15.08
RC   pPD16.51 from pPD8.33 & pPD15.08
RC   pPD18.32 from pPD16.51 & oligo
RC   pPD21.28 from pPD18.32 & pPD16.43
RC   pPD22.11 from pPD21.28
RC   pPD22.04 from pPD21.28
RC   pPD16.01 from pPD16.43 & pPD18.32
RC   pPD25.47 from pBluescript KS+ & glp-1 gene
RC   pPD26.77 from pPD16.43 & pPD25.47
RC   pPD34.110 from pPD21.28 & oligo
RA   Fire A.Z., Harrison S., Dixon D.;
RT   "A modular set of lacZ fusion vectors for studying gene expression
RT   in Caenorhabditis elegans";
RL   Gene 93:189-198(1990).
XX
RN   [2]
RC   pPD21.28
RA   Fire A.Z.;
RT   ;
RL   Submitted (11-MAY-1990) to GenBank on computer-readable media by:
RL   Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL   115 West University Parkway, Baltimore MD 21210, USA.
XX
CC   NM (pPD21.28)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pBR322)
CC   BR (pPD16.01)(pPD16.43)(pPD16.51)(pPD18.32)(pPD1.27)(pPD22.04)
CC   BR (pPD22.11)(pPD26.14)(pPD26.77)(pPD34.110)(pPD8.02)(pPD8.33)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pPD18.32 HindIII-DraI 4059bp 19..4078,
FT                   \ lacZ gene
FT                   \ DraI = 4078 4117 5282 5974 5993
FT                   \ HindIII = 19
FT                   2. pPD16.43 remove HindIII-StuI 3226bp 19..3245,
FT                   \ MCS/2504bp
FT                   -> pPD21.28 6563bp"
FT   misc_recomb     57..58
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     180..181
FT                   /note="synthetic end/E. coli trpS start"
FT   misc_recomb     264..265
FT                   /note="E. coli trpS end/synthetic start"
FT   misc_recomb     268..269
FT                   /note="synthetic end/E. coli lacZ start"
FT   misc_recomb     3262..3263
FT                   /note="E. coli lacZ end/synthetic start"
FT   misc_recomb     3322..3323
FT                   /note="synthetic end/unknown DNA start"
FT   misc_recomb     4077..4078
FT                   /note="unknown DNA end/synthetic start"
FT   misc_recomb     4097..4098
FT                   /note="synthetic end/pUC19 start"
FT   misc_recomb     4346..4347
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     4357..4358
FT                   /note="synthetic end/pUC19 start"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 6563 BP; 1587 A; 1650 C; 1723 G; 1603 T; 0 other;
     atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggat
     tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
     gaggaccctt ggagggtacc gagctcagaa aaaatgactg ctccaaagaa gaagcgtaag
     gtaccggtgg gtgaagacca gaaacagcac ctcgaactga gccgcgatat tgcccagcgt
     ttcaacgcgc tgtatggcga gatcgatccc gtcgttttac aacgtcgtga ctgggaaaac
     cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt
     cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc
     atctacacca acgtaaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat
     ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag
     acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg
     gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc
     gccggagaaa accgcctcgc ggtgatggtg ctgcgttgga gtgacggcag ttatctggaa
     gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg
     actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct
     gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt
     tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt
     atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg
     aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc
     gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa
     aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag
     catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg
     atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg
     tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac
     ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa
     cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg
     gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc
     gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt
     atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg
     tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac
     gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag
     tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat
     gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat
     cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg
     gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg
     accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg
     gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag
     ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta
     cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcctggcag
     cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca cgccatcccg
     catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg ttggcaattt
     aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg
     ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg
     acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc
     gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct gattacgacc
     gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt
     gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga tacaccgcat
     ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt aaactggctc
     ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg
     gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc
     tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac
     atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg
     gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc
     tggagcccgt cagtatcggc ggaattccaa ctgagcgccg gtcgctacca ttaccaactt
     gtctggtgtc aaaaataata ggggccgctg tcatcagatc gccatctcgc gcccgtgcct
     ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc
     accccctatt tttgttatta tcaaaaaaac ttcttcttaa tttctttgtt ttttagcttc
     ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata
     aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta
     cacaatgttc tgtgtacact tcttatgttt tttttacttc tgataaattt tttttgaaac
     atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg
     caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa
     aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa attaattttc
     ctgcttttgc tttttggggg tttcccctat tgtttgtcaa gagtttcgag gacggcgttt
     ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg
     ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta
     tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct
     actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
     ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     acaatttcac acaggaaaca gct
//