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ID   PPD2204    preliminary; circular DNA; SYN; 6562 BP.
XX
AC   M34303;
XX
DT   03-JUL-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pPD22.04 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6562
RC   pPD1.27 from pAST19b & pCH110 & oligo
RC   pPD8.02 from pPD1.27 & oligo
RC   pPD8.33 from pPD8.02 & C.elegans unc-54 gene
RC   pPD15.08 from pPD8.02
RC   pPD16.43 from pPD8.02 & pPD15.08
RC   pPD16.51 from pPD8.33 & pPD15.08
RC   pPD18.32 from pPD16.51 & oligo
RC   pPD21.28 from pPD18.32 & pPD16.43
RC   pPD22.11 from pPD21.28
RC   pPD22.04 from pPD21.28
RC   pPD16.01 from pPD16.43 & pPD18.32
RC   pPD25.47 from pBluescript KS+ & glp-1 gene
RC   pPD26.77 from pPD16.43 & pPD25.47
RC   pPD34.110 from pPD21.28 & oligo
RA   Fire A.Z., Harrison S., Dixon D.;
RT   "A modular set of lacZ fusion vectors for studying gene expression
RT   in Caenorhabditis elegans";
RL   Gene 93:189-198(1990).
XX
RN   [2]
RC   pPD22.04
RA   Fire A.Z.;
RT   ;
RL   Submitted (11-MAY-1990) to GenBank on computer-readable media by:
RL   Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL   115 West University Parkway, Baltimore MD 21210, USA.
XX
CC   NM (pPD22.04)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pBR322)
CC   BR (pPD16.01)(pPD16.43)(pPD16.51)(pPD18.32)(pPD21.28)(pPD1.27)
CC   BR (pPD22.11)(pPD26.14)(pPD26.77)(pPD34.110)(pPD8.02)(pPD8.33)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pPD21.28 StyI 6563bp 128..128
FT                   T4 DNA polymerase
FT                   -> pPD22.04 6562bp"
FT   misc_recomb     57..58
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     179..180
FT                   /note="synthetic end/E. coli trpS start"
FT   misc_recomb     263..264
FT                   /note="E. coli trpS end/synthetic start"
FT   misc_recomb     267..268
FT                   /note="synthetic end/E. coli lacZ start"
FT   misc_recomb     3261..3262
FT                   /note="E. coli lacZ end/synthetic start"
FT   misc_recomb     3321..3322
FT                   /note="synthetic end/unknown DNA start"
FT   misc_recomb     4076..4077
FT                   /note="unknown DNA end/synthetic start"
FT   misc_recomb     4096..4097
FT                   /note="synthetic end/pUC19 start"
FT   misc_recomb     4345..4346
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     4356..4357
FT                   /note="synthetic end/pUC19 start"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 6562 BP; 1587 A; 1650 C; 1722 G; 1603 T; 0 other;
     atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggat
     tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
     gaggaccctt gagggtaccg agctcagaaa aaatgactgc tccaaagaag aagcgtaagg
     taccggtggg tgaagaccag aaacagcacc tcgaactgag ccgcgatatt gcccagcgtt
     tcaacgcgct gtatggcgag atcgatcccg tcgttttaca acgtcgtgac tgggaaaacc
     ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc
     ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca
     tctacaccaa cgtaacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc
     cgacgggttg ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga
     cgcgaattat ttttgatggc gttaactcgg cgtttcatct gtggtgcaac gggcgctggg
     tcggttacgg ccaggacagt cgtttgccgt ctgaatttga cctgagcgca tttttacgcg
     ccggagaaaa ccgcctcgcg gtgatggtgc tgcgttggag tgacggcagt tatctggaag
     atcaggatat gtggcggatg agcggcattt tccgtgacgt ctcgttgctg cataaaccga
     ctacacaaat cagcgatttc catgttgcca ctcgctttaa tgatgatttc agccgcgctg
     tactggaggc tgaagttcag atgtgcggcg agttgcgtga ctacctacgg gtaacagttt
     ctttatggca gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta
     tcgatgagcg tggtggttat gccgatcgcg tcacactacg tctgaacgtc gaaaacccga
     aactgtggag cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg cacaccgccg
     acggcacgct gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa
     atggtctgct gctgctgaac ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc
     atcatcctct gcatggtcag gtcatggatg agcagacgat ggtgcaggat atcctgctga
     tgaagcagaa caactttaac gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt
     acacgctgtg cgaccgctac ggcctgtatg tggtggatga agccaatatt gaaacccacg
     gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg gctaccggcg atgagcgaac
     gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg
     ggaatgaatc aggccacggc gctaatcacg acgcgctgta tcgctggatc aaatctgtcg
     atccttcccg cccggtgcag tatgaaggcg gcggagccga caccacggcc accgatatta
     tttgcccgat gtacgcgcgc gtggatgaag accagccctt cccggctgtg ccgaaatggt
     ccatcaaaaa atggctttcg ctacctggag agacgcgccc gctgatcctt tgcgaatacg
     cccacgcgat gggtaacagt cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt
     atccccgttt acagggcggc ttcgtctggg actgggtgga tcagtcgctg attaaatatg
     atgaaaacgg caacccgtgg tcggcttacg gcggtgattt tggcgatacg ccgaacgatc
     gccagttctg tatgaacggt ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg
     aagcaaaaca ccagcagcag tttttccagt tccgtttatc cgggcaaacc atcgaagtga
     ccagcgaata cctgttccgt catagcgata acgagctcct gcactggatg gtggcgctgg
     atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt
     tgattgaact gcctgaacta ccgcagccgg agagcgccgg gcaactctgg ctcacagtac
     gcgtagtgca accgaacgcg accgcatggt cagaagccgg gcacatcagc gcctggcagc
     agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc
     atctgaccac cagcgaaatg gatttttgca tcgagctggg taataagcgt tggcaattta
     accgccagtc aggctttctt tcacagatgt ggattggcga taaaaaacaa ctgctgacgc
     cgctgcgcga tcagttcacc cgtgcaccgc tggataacga cattggcgta agtgaagcga
     cccgcattga ccctaacgcc tgggtcgaac gctggaaggc ggcgggccat taccaggccg
     aagcagcgtt gttgcagtgc acggcagata cacttgctga tgcggtgctg attacgaccg
     ctcacgcgtg gcagcatcag gggaaaacct tatttatcag ccggaaaacc taccggattg
     atggtagtgg tcaaatggcg attaccgttg atgttgaagt ggcgagcgat acaccgcatc
     cggcgcggat tggcctgaac tgccagctgg cgcaggtagc agagcgggta aactggctcg
     gattagggcc gcaagaaaac tatcccgacc gccttactgc cgcctgtttt gaccgctggg
     atctgccatt gtcagacatg tataccccgt acgtcttccc gagcgaaaac ggtctgcgct
     gcgggacgcg cgaattgaat tatggcccac accagtggcg cggcgacttc cagttcaaca
     tcagccgcta cagtcaacag caactgatgg aaaccagcca tcgccatctg ctgcacgcgg
     aagaaggcac atggctgaat atcgacggtt tccatatggg gattggtggc gacgactcct
     ggagcccgtc agtatcggcg gaattccaac tgagcgccgg tcgctaccat taccaacttg
     tctggtgtca aaaataatag gggccgctgt catcagatcg ccatctcgcg cccgtgcctc
     tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca
     ccccctattt ttgttattat caaaaaaact tcttcttaat ttctttgttt tttagcttct
     tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa
     aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac
     acaatgttct gtgtacactt cttatgtttt ttttacttct gataaatttt ttttgaaaca
     tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc
     aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa
     aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc
     tgcttttgct ttttgggggt ttcccctatt gtttgtcaag agtttcgagg acggcgtttt
     tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg
     gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat
     ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttccta
     ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag
     gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     caatttcaca caggaaacag ct
//