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ID   PPD2211    preliminary; circular DNA; SYN; 6567 BP.
XX
AC   M34304;
XX
DT   03-JUL-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pPD22.11 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6567
RC   pPD1.27 from pAST19b & pCH110 & oligo
RC   pPD8.02 from pPD1.27 & oligo
RC   pPD8.33 from pPD8.02 & C.elegans unc-54 gene
RC   pPD15.08 from pPD8.02
RC   pPD16.43 from pPD8.02 & pPD15.08
RC   pPD16.51 from pPD8.33 & pPD15.08
RC   pPD18.32 from pPD16.51 & oligo
RC   pPD21.28 from pPD18.32 & pPD16.43
RC   pPD22.11 from pPD21.28
RC   pPD22.04 from pPD21.28
RC   pPD16.01 from pPD16.43 & pPD18.32
RC   pPD25.47 from pBluescript KS+ & glp-1 gene
RC   pPD26.77 from pPD16.43 & pPD25.47
RC   pPD34.110 from pPD21.28 & oligo
RA   Fire A.Z., Harrison S., Dixon D.;
RT   "A modular set of lacZ fusion vectors for studying gene expression
RT   in Caenorhabditis elegans";
RL   Gene 93:189-198(1990).
XX
RN   [2]
RC   pPD22.11
RA   Fire A.Z.;
RT   ;
RL   Submitted (11-MAY-1990) to GenBank on computer-readable media by:
RL   Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL   115 West University Parkway, Baltimore MD 21210, USA.
XX
CC   NM (pPD22.11)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pBR322)
CC   BR (pPD16.01)(pPD16.43)(pPD16.51)(pPD18.32)(pPD21.28)(pPD22.04)
CC   BR (pPD1.27)(pPD26.14)(pPD26.77)(pPD34.110)(pPD8.02)(pPD8.33)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pPD21.28 StyI 6563bp 128..128
FT                   mung bean nuclease
FT                   -> pPD22.11 6567bp"
FT   misc_recomb     57..58
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     184..185
FT                   /note="synthetic end/E. coli trpS start"
FT   misc_recomb     268..269
FT                   /note="E. coli trpS end/synthetic start"
FT   misc_recomb     272..273
FT                   /note="synthetic end/E. coli lacZ start"
FT   misc_recomb     3266..3267
FT                   /note="E. coli lacZ end/synthetic start"
FT   misc_recomb     3326..3327
FT                   /note="synthetic end/unknown DNA start"
FT   misc_recomb     4081..4082
FT                   /note="unknown DNA end/synthetic start"
FT   misc_recomb     4101..4102
FT                   /note="synthetic end/pUC19 start"
FT   misc_recomb     4350..4351
FT                   /note="pUC19 end/synthetic start"
FT   misc_recomb     4361..4362
FT                   /note="synthetic end/pUC19 start"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 6567 BP; 1587 A; 1651 C; 1724 G; 1605 T; 0 other;
     atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggat
     tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
     gaggaccctt gcttggaggg taccgagctc agaaaaaatg actgctccaa agaagaagcg
     taaggtaccg gtgggtgaag accagaaaca gcacctcgaa ctgagccgcg atattgccca
     gcgtttcaac gcgctgtatg gcgagatcga tcccgtcgtt ttacaacgtc gtgactggga
     aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
     tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
     gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga
     gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
     ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg
     ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt
     acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct
     ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa
     accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg
     cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac
     agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga
     aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa
     cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac
     cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat
     tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca
     cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct
     gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct
     gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac
     ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag
     cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc
     gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc
     tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga
     tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa
     atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga
     atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg
     tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa
     atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa
     cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct
     gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga
     agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc
     gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa
     acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac
     agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg
     gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat
     cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca
     atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct
     gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga
     agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca
     ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac
     gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg
     gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc
     gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg
     gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg
     ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct
     gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt
     caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca
     cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga
     ctcctggagc ccgtcagtat cggcggaatt ccaactgagc gccggtcgct accattacca
     acttgtctgg tgtcaaaaat aataggggcc gctgtcatca gatcgccatc tcgcgcccgt
     gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc
     tcccaccccc tatttttgtt attatcaaaa aaacttcttc ttaatttctt tgttttttag
     cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt
     aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa
     tctacacaat gttctgtgta cacttcttat gtttttttta cttctgataa attttttttg
     aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg
     accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg
     tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat
     tttcctgctt ttgctttttg ggggtttccc ctattgtttg tcaagagttt cgaggacggc
     gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg
     tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg
     tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt
     tcctactagt cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg cctcgtgata cgcctatttt
     tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     gataacaatt tcacacagga aacagct
//